CN211814400U

CN211814400U - A reagent pipe support for peripheral blood DNA draws

Info

Publication number: CN211814400U
Application number: CN202020119954.5U
Authority: CN
Inventors: 刘晓丽; 王敬仁
Original assignee: Liuzhou Maternity and Child Healthcare Hospital
Current assignee: Liuzhou Maternity and Child Healthcare Hospital
Priority date: 2020-01-19
Filing date: 2020-01-19
Publication date: 2020-10-30
Anticipated expiration: 2030-01-19

Abstract

The utility model belongs to the technical field of the experimental articles for use technique and specifically relates to a reagent pipe support for extraction of peripheral blood DNA, including fixed plate, shielding plate and the mechanism that opens and shuts, the fixed plate has been laid a plurality of mouths of placing of two sides about running through, the fixed plate be equipped with four dead levers all around at least, the dead lever is all fixed to be worn to locate the fixed plate, the shielding plate sets up the top of fixed plate, and sliding sleeve locates on the dead lever, the shielding plate be equipped with place the sample addition mouth that the mouth was aimed at, be equipped with the cavity in the shielding plate, the quantity of the mechanism that opens and shuts with place the quantity of mouth the same, and locate in the shielding plate, the mechanism that opens and shuts is used for control opening and shutting of sample addition mouth. The utility model discloses can prevent effectively that the DNA of peripheral blood from drawing the pollution of in-process peripheral blood to the reagent, guarantee the accuracy of testing result.

Description

A reagent pipe support for peripheral blood DNA draws

Technical Field

The utility model belongs to the technical field of the experimental articles for use technique and specifically relates to a reagent pipe support for peripheral blood DNA draws.

Background

Peripheral blood is blood except bone marrow, and some methods are commonly used clinically to release hematopoietic stem cells in the bone marrow into the blood, and then hematopoietic stem cells are extracted and separated from the blood, and the stem cells obtained in this way are called peripheral blood stem cells, and the new concept of peripheral blood is proposed for the first time in the life ark program started in humans in the early twenty-first century.

In the process of extracting the DNA of the peripheral blood, the reagent on the reagent rack is opened, the peripheral blood is easy to splash when being added to the reagent hole, and the residual peripheral blood in the suction head is easy to drip down in the process of throwing the suction head to the waste liquid waste gas barrel after the sample addition is finished, thereby polluting the reagent on the reagent rack. Therefore, the reagent is easily polluted by the peripheral blood in the process of extracting the DNA of the peripheral blood, and the accuracy of subsequent experiments and detection results is influenced.

SUMMERY OF THE UTILITY MODEL

In order to solve the problem, the utility model provides a reagent pipe support for peripheral blood DNA draws can prevent effectively that the DNA of peripheral blood from drawing the pollution of in-process peripheral blood to the reagent, guarantees the accuracy of testing result.

In order to realize the purpose, the utility model discloses a technical scheme be:

a reagent tube rack for extracting peripheral blood DNA, which comprises a fixing plate, a baffle plate and an opening and closing mechanism,

the fixing plate is provided with a plurality of placing openings penetrating through the upper surface and the lower surface, at least four fixing rods are arranged around the fixing plate, the fixing rods are fixedly arranged on the fixing plate in a penetrating manner, the shielding plate is arranged above the fixing plate and is sleeved on the fixing rods in a sliding manner, a cavity is arranged in the shielding plate, the shielding plate is provided with a sample adding opening aligned with the placing openings, and the sample adding opening is communicated with the cavity of the shielding plate;

the number of the opening and closing mechanisms is the same as that of the placing openings and is located in the shielding plates, each opening and closing mechanism comprises two shielding pieces, a rack and a knob, the two shielding pieces are located on the shielding plates and are oppositely arranged on two sides of the sample adding opening, the two shielding pieces are connected with the shielding plates in a sliding mode, and the sample adding opening can be closed and opened by sliding the two shielding pieces; the two racks are positioned in the shielding plate, the two racks are parallel to the sliding direction of the shielding piece and are positioned on one side of the sample adding port, one end of each rack is fixedly connected with the two shielding pieces, and the other end of each rack faces the same direction; a gear is arranged between the two racks, the gear is meshed with the two racks, and two ends of the gear are respectively in rotating connection with the upper inner wall and the lower inner wall in the shielding plate; the knob is arranged on the shielding plate, and one end of the knob is rotatably arranged in the shielding plate in a penetrating mode and fixedly connected with the gear.

Furthermore, a base is arranged on one side, back to the shielding plate, of the fixing plate, and the base is fixedly connected with the fixing rod.

Furthermore, a plurality of fixing grooves are concavely arranged on one surface of the base facing the fixing plate, and the fixing grooves are aligned with the placing openings.

Furthermore, at least four suckers are arranged on one surface of the base, which faces away from the fixing plate.

The shielding plate fixing device further comprises a locking nut, wherein the locking nut is in threaded connection with the fixing rod and can be abutted against one surface, back to the fixing plate, of the shielding plate, so that the shielding plate can be fixed above the fixing plate.

Furthermore, two the shielding piece all is equipped with the slider, the shielding plate be equipped with slider sliding fit's spout, the shielding piece pass through the slider with spout sliding connection.

Furthermore, two the shielding piece all is equipped with the bayonet socket, the bayonet socket can be followed the perpendicular to shielding piece slip direction mutual joint cooperation.

Furthermore, two the shielding part joint complex one end all coats and has sealed glue.

The utility model has the advantages that:

1. inserting the reagent tube into the placing opening, so that the periphery of the opening end of the reagent tube can be abutted against the peripheral opening of the placing opening, and the reagent tube can be placed on the fixing plate; the shielding plate is sleeved on the fixing rod, so that the shielding plate can be arranged above the fixing plate. Under the effect of mechanism that opens and shuts, through twisting the knob, the gear is driven, because the gear setting is between two racks, under the drive of gear, two racks move along opposite direction to make two racks can drive the shielding piece that corresponds respectively and slide along opposite direction, realized the closure or the opening of application of sample mouth. When the experimenter need add peripheral blood in the suction head to the reagent intraductal, the accessible is twisted and is moved the knob that corresponds with this reagent pipe for this sample addition port is opened, and all the other sample addition ports are sealed by the shielding piece, thereby prevented that the unexpected drippage of peripheral blood of suction head is in all the other reagent pipes, can guarantee that the reagent in the reagent pipe can not receive the pollution, in order to ensure testing result's accuracy.

2. Under the action of the base, the reagent tube can be protected, and the reagent tube is prevented from accidentally sliding out of the placing opening; and in reagent pipe can imbed the fixed slot, prevent that reagent pipe from appearing rocking and leading to the intraductal reagent of reagent to spill, guaranteed the cleanness of laboratory bench.

3. Under the effect of bayonet socket, can improve the leakproofness between two shielding parts, prevent that the peripheral blood that the suction head dripped from oozing to the reagent intraductal from between two shielding parts. Under the action of the sealant, the sealing performance of the two shielding pieces when the two shielding pieces are closed can be further improved.

Drawings

FIG. 1 is a schematic diagram of the structure of a reagent tube rack for peripheral blood DNA extraction according to a preferred embodiment of the present invention.

FIG. 2 is a schematic structural view of the opening and closing mechanism of the reagent tube holder for peripheral blood DNA extraction according to a preferred embodiment of the present invention.

FIG. 3 is a schematic view of the shielding plate structure of the reagent tube holder for peripheral blood DNA extraction according to a preferred embodiment of the present invention.

In the figure, 1-a fixing plate, 11-a placing port, 12-a fixing rod, 2-a baffle plate, 201-a sliding chute, 21-a sample adding port, 22-a locking nut, 31-a baffle piece, 311-a sliding piece, 312-a bayonet, 313-sealant, 32-a rack, 33-a knob, 34-a gear, 4-a base, 41-a fixing slot, 42-a sucker and 5-a reagent tube.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When a component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components may also be present. When a component is referred to as being "disposed on" another component, it can be directly on the other component or intervening components may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Referring to fig. 1 to 3, the reagent tube rack for peripheral blood DNA extraction according to a preferred embodiment of the present invention includes a fixing plate 1, a shielding plate 2 and an opening and closing mechanism.

Fixed plate 1 has laid a plurality of openings 11 of placing that run through the upper and lower two sides, and fixed plate 1 is equipped with four dead levers 12 all around at least, and fixed plate 1 is worn to locate all fixed rods 12, and shielding plate 2 sets up in fixed plate 1's top, and sliding sleeve locates on dead lever 12, is equipped with the cavity in shielding plate 2, and shielding plate 2 set up and place the sample port 21 that opening 11 was aimed at, and sample port 21 switches on with sample port 21.

The reagent tube 5 is inserted into the placing port so that the peripheral edge of the open end of the reagent tube 5 can abut against the peripheral edge of the placing port 11, thereby allowing the reagent tube 5 to be placed on the fixing plate 1.

In this embodiment, a base 4 is disposed on a surface of the fixing plate 1 opposite to the shielding plate 2, and the base 4 is fixedly connected to the fixing rod 12. Under the action of the base 4, the reagent tube 5 can be protected, and the reagent tube 5 is prevented from accidentally sliding out of the placing opening 11.

Preferably, a plurality of fixing grooves 41 are concavely formed on a surface of the base 4 facing the fixing plate 1, and the fixing grooves 41 are aligned with the placing hole 11. Reagent pipe 5 can be embedded into fixed slot 41 in, prevent that reagent pipe 5 from appearing rocking and leading to the reagent effluvium in the reagent pipe 5, has guaranteed the cleanness of laboratory bench.

The surface of the base 4 opposite to the fixing plate 1 is provided with four suckers 42. Under the effect of sucking disc 42, can fix base 4 on the laboratory bench, prevent the unexpected slip of in-process base 4 of experiment, guarantee going on smoothly of experiment.

The number of the opening and closing mechanisms is the same as that of the placing openings 11, and the opening and closing mechanisms are all located in the shielding plate 2, and each opening and closing mechanism comprises a shielding piece 31, a rack 32 and a knob 33.

The shielding pieces 31 are provided with two shielding plates 2, the two shielding pieces 31 are oppositely arranged on two sides of the sample adding port 21, the two shielding pieces 31 are connected with the shielding plates 2 in a sliding mode, and the sample adding port 21 can be closed and opened through sliding the two shielding pieces 31. Two racks 32 are arranged in the shielding plate 2, the two racks 32 are parallel to the sliding direction of the shielding piece 31 and are both arranged at one side of the sample addition port 21, one end of each rack 32 is fixedly connected with the two shielding pieces 31, and the other ends of the racks face the same direction. A gear 34 is arranged between the two racks 32, the gear 34 is meshed with the two racks 32, and two ends of the gear are respectively connected with the upper inner wall and the lower inner wall of the shielding plate 2 in a rotating way; the knob 33 is disposed on the shielding plate 2, and one end of the knob 33 is rotatably disposed through the shielding plate 2 and fixedly connected to the gear 34.

Under the effect of the opening and closing mechanism, the knob 33 is screwed, the gear 34 is driven, and the gear 34 is arranged between the two racks 32, and the two racks 32 move along opposite directions under the driving of the gear 34, so that the two racks 32 can respectively drive the corresponding shielding pieces 31 to slide along opposite directions, and the closing or opening of the sample addition port 21 is realized.

In this embodiment, when rotating knob 33 clockwise, two shielding parts 31 move towards the direction of keeping away from sample addition port 21 center for sample addition port 21 can communicate with fixed mouthful 11, the experimenter can add the peripheral blood in the tip into corresponding reagent tube 5, and shielding part 31 in remaining sample addition port 21 is closed state, thereby realized preventing that the peripheral blood of tip from accidentally dropping into in remaining reagent tube 5, can guarantee that the reagent in the reagent tube can not receive the pollution, in order to ensure the accuracy of testing result. After the extraction of the peripheral blood DNA is completed, the knob 33 is rotated counterclockwise, and the two shutters 31 move toward the center of the sample addition port 21, so that the sample addition port 21 can be closed by the two shutters 31.

In this embodiment, the two shielding members 31 are both provided with a sliding member 311, the shielding plate 2 is provided with a sliding slot 201 in sliding fit with the sliding member 311, and the shielding members 31 are slidably connected with the sliding slot 201 through the sliding member 311. Under the action of the slider 311 and the slide groove 201, both the racks 32 can be kept in a meshed state with the racks 32, and the opening and closing of the sample addition port 21 can be ensured to be smoothly performed.

Preferably, both the shields 31 are provided with a bayonet 312, the bayonet 312 being snap-fittable to each other in a direction perpendicular to the sliding direction of the shields 31. By the bayonet 312, the sealing performance between the two shields 31 can be improved, and peripheral blood dripping from the tip can be prevented from leaking into the reagent tube 5 from between the two shields 31. One end of each of the two blocking pieces 31 in clamping fit is coated with a sealant 313. The sealing performance of the both shutters 31 at the time of closing can be further improved by the sealant 313.

The present embodiment further includes a locking nut 22, wherein the locking nut 22 is screwed to the fixing rod 12 and can abut against a surface of the shielding plate 2 opposite to the fixing plate 1, so that the shielding plate 2 can be fixed above the fixing plate 1. When the shielding plate 2 is sleeved on the fixing rod 12, the locking nut 22 is screwed, so that the shielding plate 2 can be fixed above the fixing plate 1, and the shielding plate 2 is prevented from being overturned due to mistaken touch in the experiment process.

When the reagent tube holder for peripheral blood DNA extraction of the present embodiment is used, the blocking plate 2 is detached from the fixing plate 1 after the locking nut 22 is screwed out of the fixing rod 12; then, the reagent vessel 5 is inserted into the fixing groove 41 of the base 4 through the placing port 11, and the reagent is added to the reagent vessel 5. After the reagent is added, the shutter 2 can be fixed above the fixing plate 1 by screwing the lock nut 22.

When the experimenter needs to extract the peripheral blood DNA, the DNA to be extracted is absorbed by the suction head, the reagent tube 5 required to be used is rotated clockwise and corresponds to the corresponding knob 33, the gear 34 is driven, under the driving of the gear 34, the two racks 32 move in opposite directions, and the two shielding pieces 31 move towards the direction far away from the center of the sample adding port 21, so that the sample adding port 21 can be communicated with the fixed port 11, the experimenter can add the peripheral blood in the suction head into the corresponding reagent tube 5, and the shielding pieces 31 in the rest sample adding port 21 are in a closed state. After the extraction of the peripheral blood DNA is completed, the knob 33 is rotated counterclockwise, and the two shutters 31 move toward the center of the sample addition port 21, so that the sample addition port 21 can be closed by the two shutters 31.

Claims

1. A reagent tube rack for peripheral blood DNA extraction is characterized by comprising a fixing plate (1), a baffle plate (2) and an opening and closing mechanism,

the fixing plate (1) is provided with a plurality of placing openings (11) penetrating through the upper surface and the lower surface, at least four fixing rods (12) are arranged around the fixing plate (1), the fixing rods (12) are fixedly arranged on the fixing plate (1) in a penetrating manner, the shielding plate (2) is arranged above the fixing plate (1) and is sleeved on the fixing rods (12) in a sliding manner, a cavity is arranged in the shielding plate (2), the shielding plate (2) is provided with a sample adding opening (21) aligned with the placing openings (11), and the sample adding opening (21) is communicated with the cavity of the shielding plate (2);

the number of the opening and closing mechanisms is the same as that of the placing openings (11), and the opening and closing mechanisms are all located in the shielding plates (2), each opening and closing mechanism comprises shielding pieces (31), racks (32) and knobs (33), the two shielding pieces (31) are arranged and are located in the shielding plates (2), the two shielding pieces (31) are oppositely arranged on two sides of the sample adding opening (21), the two shielding pieces (31) are connected with the shielding plates (2) in a sliding mode, and the sample adding opening (21) can be closed and opened by sliding the two shielding pieces (31); the two racks (32) are positioned in the shielding plate (2), the two racks (32) are parallel to the sliding direction of the shielding piece (31) and are positioned on one side of the sample adding port (21), one ends of the two racks (32) are fixedly connected with the two shielding pieces (31) respectively, and the other ends of the two racks face the same direction; a gear (34) is arranged between the two racks (32), the gear (34) is meshed with the two racks (32), and two ends of the gear are respectively connected with the upper inner wall and the lower inner wall of the shielding plate (2) in a rotating manner; the knob (33) is arranged on the shielding plate (2), and one end of the knob (33) is rotatably arranged in the shielding plate (2) in a penetrating manner and is fixedly connected with the gear (34).

2. The reagent tube holder for peripheral blood DNA extraction according to claim 1, characterized in that: the fixing plate (1) is provided with a base (4) on one side back to the shielding plate (2), and the base (4) is fixedly connected with the fixing rod (12).

3. The reagent tube holder for peripheral blood DNA extraction according to claim 2, characterized in that: one surface of the base (4) facing the fixing plate (1) is concavely provided with a plurality of fixing grooves (41), and the fixing grooves (41) are aligned to the placing openings (11).

4. The reagent tube holder for peripheral blood DNA extraction according to claim 2, characterized in that: the base (4) is at least provided with four suckers (42) on one surface back to the fixing plate (1).

5. The reagent tube holder for peripheral blood DNA extraction according to claim 1, characterized in that: the fixing device is characterized by further comprising a locking nut (22), wherein the locking nut (22) is in threaded connection with the fixing rod (12) and can be abutted against one surface, back to the fixing plate (1), of the shielding plate (2), so that the shielding plate (2) can be fixed above the fixing plate (1).

6. The reagent tube holder for peripheral blood DNA extraction according to claim 1, characterized in that: the two shielding pieces (31) are respectively provided with a sliding piece (311), the shielding plate (2) is provided with a sliding groove (201) in sliding fit with the sliding piece (311), and the shielding pieces (31) are in sliding connection with the sliding grooves (201) through the sliding pieces (311).

7. The reagent tube holder for peripheral blood DNA extraction according to claim 1, characterized in that: two shielding piece (31) all is equipped with bayonet socket (312), bayonet socket (312) can be followed the perpendicular to shielding piece (31) slip direction mutual joint cooperation.

8. The reagent tube holder for peripheral blood DNA extraction according to claim 7, characterized in that: one end of the blocking piece (31) in clamping fit is coated with sealant (313).