CN211785091U - Optical fiber biochemical sensor - Google Patents
Optical fiber biochemical sensor Download PDFInfo
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- CN211785091U CN211785091U CN202020202585.6U CN202020202585U CN211785091U CN 211785091 U CN211785091 U CN 211785091U CN 202020202585 U CN202020202585 U CN 202020202585U CN 211785091 U CN211785091 U CN 211785091U
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Abstract
The utility model discloses an optical fiber biochemical sensor, which relates to the technical field of optical fiber sensing, and comprises a single mode optical fiber and a functional group, wherein the single mode optical fiber comprises a fiber core and a cladding, and the fiber core is carved with a Bragg fiber grating which is distributed along the axial direction of the optical fiber; the cladding corresponding to the Bragg fiber grating is partially or completely etched until the fiber core forms at least one groove; the fiber Bragg grating forms a phase-shift fiber grating under the modulation action of the at least one groove; the functional groups are laid at the bottom of the groove; the functionalized group is combined with a biological recognition object or a chemical recognition object. The utility model provides a detection of biochemical substance concentration can be realized to optic fibre biochemical sensor. The optical fiber biochemical sensor can eliminate the temperature crosstalk of the sensor and improve the detection precision.
Description
Technical Field
The utility model belongs to the technical field of the optical fiber sensing, in particular to optical fiber biochemical sensor.
Background
A biochemical sensor refers to a device or apparatus that can sense (or respond to) biological and chemical quantities and convert them into usable signals (including electrical signals, optical signals, etc.) according to a certain rule for output. Biochemical sensors are typically the product of a cross-discipline of many disciplines, involving a number of fields, biological, chemical, medical, physical, electronic technologies, and so on. At present, biochemical sensors are widely applied in the fields of biomedicine, clinical diagnosis, drug analysis, food detection, environmental monitoring, military biochemical detection and the like.
The optical fiber sensing technology is a novel sensing technology which is rapidly developed in the last 70 th century along with the optical fiber technology and the optical communication technology. At present, the optical fiber biochemical sensor is in a preliminary research and development stage. Compared with other traditional biochemical sensors, the optical fiber biochemical sensor has the following unique advantages: the volume is small, the flexibility is good, the electromagnetic interference is resisted, the high temperature resistance, the corrosion resistance, the strong multiplexing capability and the high information transmission capacity are realized, the multi-parameter and multi-channel measurement can be realized, and the cost is favorably reduced. However, the existing optical fiber biochemical sensor is easily interfered by the external environment temperature in the actual biochemical detection application, and has the problems of low detection precision and the like.
Disclosure of Invention
In order to overcome the defects of the prior art, the utility model aims to provide an optical fiber biochemical sensor which can eliminate temperature crosstalk and improve detection precision.
The utility model provides an optical fiber biochemical sensor, which comprises a single mode optical fiber and a functional group, wherein the single mode optical fiber comprises a fiber core and a cladding, and the fiber core is carved with a Bragg fiber grating which is distributed along the axial direction of the optical fiber; the cladding corresponding to the Bragg fiber grating is partially or completely etched until the fiber core forms at least one groove; the fiber Bragg grating forms a phase-shift fiber grating under the modulation action of the at least one groove; the functional groups are laid at the bottom of the groove; the functionalized group is combined with a biological recognition object or a chemical recognition object.
When the fiber biochemical sensor works, when an object to be detected in an external environment is specifically combined with a functional group and a biochemical identification object on the surface of the bottom of the groove of the fiber biochemical sensor, the refractive index outside the fiber core in the groove is changed, and further the effective refractive index of a mode in the fiber core is changed, so that the phase shift amount of the phase shift fiber grating is changed, and the central wavelength of a defect mode in the reflection spectrum or the transmission spectrum of the phase shift fiber grating is shifted. The concentration of the object to be detected and the drift amount of the central wavelength of the defect mode are in a monotonic relation, so that the detection of the concentration of the biochemical substances can be realized by calculating the corresponding drift amount.
Because the central wavelength of the envelope of the reflection spectrum or the transmission spectrum of the whole phase-shift fiber grating is not influenced by the object to be detected, the monitoring of the environment temperature variation can be realized by measuring the drift amount of the central wavelength of the envelope of the reflection spectrum or the transmission spectrum of the whole phase-shift fiber grating and according to the monotonous relation between the drift amount and the environment temperature variation, thereby eliminating the temperature crosstalk of the sensor and improving the detection precision.
In an alternative embodiment, the single mode fiber is a photothermal single mode fiber.
In an alternative embodiment, the bottom of the groove is etched to the surface or inside of the core; when the number of the grooves is plural, the size and/or etching depth of the groove opening may be the same or different.
In an alternative embodiment, the functionalized group is chemically bonded or electrostatically adsorbed onto the bottom of the groove.
Compared with the prior art, the utility model provides an optical fiber biochemical sensor, wherein, optical fiber biochemical sensor can realize the detection of biochemical substance concentration, uses this optical fiber biochemical sensor, can eliminate the temperature crosstalk of sensor to improve detection accuracy; in addition, through at least one recess of preparation on fiber grating, if the quantity of recess is a plurality of, then can realize the simultaneous detection of a plurality of passageways on the single fiber probe, detect for the single channel, can realize higher relevance ratio through comparing the testing result of a plurality of passageways.
Drawings
Fig. 1 is a schematic structural diagram of an optical fiber biochemical sensor according to an embodiment of the present invention;
FIG. 2 is a simplified diagram of the reflection spectrum of an optical fiber biochemical sensor according to an embodiment of the present invention;
FIG. 3 is a schematic structural diagram of an optical fiber biochemical sensor according to another embodiment of the present invention;
FIG. 4 is a simplified schematic diagram of the reflection spectrum of a fiber optic biochemical sensor according to yet another embodiment of the present invention;
fig. 5 is a flowchart of a method for manufacturing an optical fiber biochemical sensor according to an embodiment of the present invention.
Detailed Description
The invention is further explained below with reference to the drawings and examples.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
Please refer to fig. 1, which is a schematic structural diagram of an optical fiber biochemical sensor according to an embodiment of the present invention.
As shown in fig. 1, the fiber optic biochemical sensor includes a single mode fiber 1 and a functional group 3. The single mode optical fiber 1 includes a core 12 and a cladding 11. The core 12 of the single mode fiber 1 is inscribed with a bragg fiber grating 13 distributed along the fiber axis. The bragg fiber grating 13 may be processed using an optical system based on an excimer laser or an optical system based on a femtosecond laser.
The cladding 11 corresponding to the bragg fiber grating 13 is partially or completely etched to the core 12 to form at least one groove 14. The bragg fiber grating 13 forms a phase-shifted fiber grating under the modulation of the grooves 14. Specifically, the bottom of the groove 14 is etched to the surface or inside of the core. The grooves 14 may be etched using a focused ion beam processing system or a femtosecond laser based processing system.
The functionalized groups 3 are laid on the bottom of the grooves 14. Specifically, the functional group is laid on the bottom of the groove 14 by chemical bond bonding or electrostatic adsorption.
The functional group 3 is bound with a biological or chemical identifier 5.
The functional group 3 can enhance the influence of an external environment medium on an optical fiber evanescent field, and on the other hand, the functional group 3 can also provide a larger attachment area for the biological recognition object or the chemical recognition object 5, so that the probability of combining the biological recognition object or the chemical recognition object 5 with a substance to be detected is increased, and the sensitivity of the sensor is improved from the two aspects. The biological recognition substance or the chemical recognition substance 5 is bonded to the functional group 3 by chemical bond bonding or electrostatic adsorption.
As shown in fig. 2, the solid line is the reflection spectrum of the fiber optic biochemical sensor according to an embodiment of the present invention, for the phase shift fiber grating formed by the bragg fiber grating 13 under the modulation effect of the groove 14, the reflection spectrum (or the transmission spectrum) thereof can generate the defect mode with a very narrow line width, the central wavelength of the resonance valley corresponding to the defect mode is sensitive to the change of the external refractive index, and the narrow line width has a very high sensing accuracy. In fig. 2, the dotted line is the reflection spectrum envelope of the reflection spectrum of the fiber optic biochemical sensor.
When the fiber grating sensor works, the fiber biochemical sensor can be connected to the fiber grating demodulator. When an object to be detected in an external environment is specifically combined with a specific functional group and a biochemical identifier on the surface of the bottom of the groove of the optical fiber biochemical sensor, the refractive index outside the fiber core in the groove is changed, and further the effective refractive index of a mode in the fiber core is changed, so that the phase shift amount of the phase-shifted optical fiber grating is changed, and the central wavelength of a defect mode in the reflection spectrum or the transmission spectrum of the phase-shifted optical fiber grating is shifted. The concentration of the object to be detected and the drift amount of the central wavelength of the defect mode are in a monotonic relation, so that the detection of the concentration of the biochemical substances can be realized by calculating the corresponding drift amount.
In addition, because the central wavelength of the envelope of the reflection spectrum or the transmission spectrum of the whole phase-shift fiber grating is not influenced by the object to be detected, the monitoring of the environment temperature variation can be realized by measuring the drift amount of the central wavelength of the envelope of the reflection spectrum or the transmission spectrum of the whole phase-shift fiber grating and according to the monotonic relation between the drift amount and the environment temperature variation, thereby eliminating the temperature crosstalk of the sensor and improving the detection precision.
On the other hand, the embodiment of the utility model provides a can optimize recess opening's size or degree of depth, for example prevent that great size nonspecific material from getting into the recess to resist biological nonspecific adsorption, allow simultaneously that soluble to be measured material granule enters into recess bottom and biological identification thing or chemical identification thing combination through the recess opening with minimum hindrance, with the sensitivity that improves the sensor, can reach the sensitivity that monomolecular detected even. It is obvious to a person skilled in the art that the optimization can be performed according to actual needs, such as the capacity of the grooves, the optical properties, etc.
The number of grooves may be plural. For example, the number of grooves may be 3, as shown in fig. 3. The optical fiber detection device can realize simultaneous detection of a plurality of channels (or a plurality of points) on a single optical fiber probe, and can realize higher detection rate by comparing detection results of the plurality of channels relative to single-channel detection. Fig. 4 is a simplified diagram of the reflection spectrum of the fiber optic biochemical sensor corresponding to 3 grooves.
Optionally, the grooves may be distributed at equal intervals, or may be distributed according to other rules.
Further, the single mode fiber 1 may be a photo-thermal single mode fiber.
The photothermal single mode fiber is also called a single mode photothermal fiber or a high attenuation fiber, and refers to a fiber that generates a thermal effect. The photothermal optical fiber can absorb light energy, convert the light energy into heat, and the amount of heat generated increases as the light energy increases. Photothermal fibers are typically implemented using doped fibers, such as cobalt-doped fibers, erbium ytterbium co-doped fibers, and the like. The photo-thermal single-mode fiber is a single-mode fiber which can generate thermal effect. Preferably, the single-mode photothermal fiber is a cobalt-doped fiber. Adopt light and heat single mode fiber, be convenient for after using optic fibre biochemical sensor to detect biochemical substance concentration, heat through light and heat single mode fiber and destroy biochemical substance, avoid this biochemical substance to cause the pollution, for example, if biochemical substance is the virus, then after detecting virus concentration, through heating the virus, can reach the effect of killing virus. When the disinfection result meets a certain condition, the sensor can be reused.
Please refer to fig. 5, which is a flowchart illustrating a method for manufacturing an optical fiber biochemical sensor according to an embodiment of the present invention. As shown in fig. 5, the manufacturing method includes:
step S201, a bragg fiber grating is etched on the single mode fiber along the fiber axis.
Specifically, step S201 includes:
and processing the Bragg fiber grating on the single-mode fiber along the fiber core direction by adopting an optical system based on an excimer laser or an optical system based on a femtosecond laser.
Step S202, etching part or all of the cladding corresponding to the Bragg fiber grating to the fiber core to form at least one groove.
The fiber bragg grating forms a phase shifted fiber grating under the modulation of the at least one groove.
Specifically, step S202 includes:
and etching the position, corresponding to the Bragg fiber grating, of the single-mode fiber from the cladding to the fiber core by adopting a focused ion beam processing system or a processing system based on a femtosecond laser to form at least one groove.
And step S203, paving the functionalized groups at the bottom of the groove.
The functional group can be a polymorphic metal nano material, a multi-type metal nano hybrid, a polymorphic metal nano hybrid, a porous structure or a hybrid of a two-dimensional material and a metal nano material.
Specifically, step S203 includes:
and plating functional groups at the bottom of the groove by adopting a chemical bond combination or electrostatic adsorption mode.
Step S204, binding the biological recognition object or the chemical recognition object on the functional group.
The biorecognition agent can be an antibody, an antigen, an enzyme or a nucleic acid.
The chemical identifier is an identifier that chemically reacts with the substance to be detected.
Specifically, step S204 includes:
and binding the biological recognition object or the chemical recognition object on the functional group by adopting a chemical bond binding or electrostatic adsorption mode.
The optical fiber biochemical sensor obtained by the manufacturing method provided by the embodiment has the technical characteristics of the optical fiber biochemical sensor in the foregoing embodiments, and details are not repeated here. The optical fiber biochemical sensor is applied to realize the multiple measurement of the biochemical sample to be detected which is introduced once, and the temperature crosstalk of the sensor can be eliminated, thereby improving the detection precision; in addition, through at least one recess of preparation on fiber grating, if the quantity of recess is a plurality of, then can realize the simultaneous detection of a plurality of passageways on the single fiber probe, detect for the single channel, can realize higher relevance ratio through comparing the testing result of a plurality of passageways.
All the possible combinations of the technical features of the above embodiments are not described for the sake of brevity, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not construed as limiting the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. The protection scope of the present invention should be subject to the appended claims.
Claims (4)
1. The optical fiber biochemical sensor is characterized by comprising a single-mode optical fiber and a functional group, wherein the single-mode optical fiber comprises a fiber core and a cladding, and the fiber core is engraved with a Bragg fiber grating distributed along the axial direction of the optical fiber; the cladding corresponding to the Bragg fiber grating is partially or completely etched until the fiber core forms at least one groove; the fiber Bragg grating forms a phase-shift fiber grating under the modulation action of the at least one groove; the functional groups are laid at the bottom of the groove; the functionalized group is combined with a biological recognition object or a chemical recognition object.
2. The fiber optic biochemical sensor according to claim 1, wherein the single mode fiber is a photothermal single mode fiber.
3. The optical fiber biochemical sensor according to claim 1, wherein the bottom of the groove is etched to the surface or the inside of the fiber core; when the number of the grooves is plural, the sizes and/or etching depths of the openings of the grooves are the same or different.
4. The optical fiber biochemical sensor according to any one of claims 1 to 3, wherein the functional group is chemically bonded or electrostatically adsorbed at the bottom of the groove.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111208070A (en) * | 2020-02-24 | 2020-05-29 | 浙江大学 | Optical fiber biochemical sensor and manufacturing method thereof |
| CN114235743A (en) * | 2021-12-20 | 2022-03-25 | 武汉理工大学 | Hydrogen detection device based on phase shift grating temperature compensation technology |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111208070A (en) * | 2020-02-24 | 2020-05-29 | 浙江大学 | Optical fiber biochemical sensor and manufacturing method thereof |
| CN111208070B (en) * | 2020-02-24 | 2024-03-29 | 浙江大学 | Optical fiber biochemical sensor and manufacturing method thereof |
| CN114235743A (en) * | 2021-12-20 | 2022-03-25 | 武汉理工大学 | Hydrogen detection device based on phase shift grating temperature compensation technology |
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