CN211741077U - Fluorescence detection circuit and fluorescence detection equipment - Google Patents
Fluorescence detection circuit and fluorescence detection equipment Download PDFInfo
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- CN211741077U CN211741077U CN202020349821.7U CN202020349821U CN211741077U CN 211741077 U CN211741077 U CN 211741077U CN 202020349821 U CN202020349821 U CN 202020349821U CN 211741077 U CN211741077 U CN 211741077U
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Abstract
The embodiment of the utility model discloses fluorescence detection circuitry and fluorescence detection equipment, this circuit include photodiode, current-voltage conversion module, filtering module and detection module, and filtering module includes first operational amplifier, and photodiode's negative pole is connected with current-voltage conversion module's input electricity, and photodiode's positive pole ground connection, current-voltage conversion module's output is connected with filtering module's input electricity, and filtering module's output is connected with detection module's input electricity. The circuit collects fluorescent signals through the photodiode and converts the fluorescent signals into current signals, the current signals are converted into voltage signals through the current-voltage conversion module and amplified, the voltage signals are filtered through the filtering module and then input into the detection module, so that the detection module determines the intensity of fluorescence according to the acquired voltage signals, and the circuit has the advantages of high detection precision, small size, low power consumption and good filtering performance and has good practicability.
Description
Technical Field
The embodiment of the utility model provides a relate to fluorescence detection area, especially relate to a fluorescence detection circuitry and fluorescence detection equipment.
Background
The measurement of the components and the content of oil in water has important significance for protecting the natural environment and reducing the influence of pollution on the production and the life of human beings.
When the ultraviolet light source with specific wavelength is used for irradiating the pretreated water sample, the oil in the water sample can be excited to generate fluorescence with specific wavelength, and the fluorescence intensity is in direct proportion to the content of the oil in the water sample. Using this principle, the amount of oil in the water can be calculated by measuring the intensity of the fluorescence produced.
Because the generated fluorescence is weak, the conventional fluorescence detection circuit usually uses a photomultiplier tube to convert the optical signal into an electrical signal. However, the photomultiplier has the disadvantages of large volume, high price, complex peripheral circuit, susceptibility to external environmental factors and the like, and does not meet the requirements of low cost, small volume and high detection precision of a fluorescence detection circuit.
SUMMERY OF THE UTILITY MODEL
An object of the embodiment of the utility model is to provide a fluorescence detection circuitry and fluorescence check out test set, this circuit can be used to detect the content of oil in the water, and has advantages such as the precision is high, with low costs and the consumption is little.
To achieve the purpose, the embodiment of the present invention adopts the following technical solutions:
a fluorescence detection circuit, comprising: the device comprises a photodiode, a current-voltage conversion module, a filtering module and a detection module; the filtering module comprises a first operational amplifier;
the cathode of the photodiode is electrically connected with the input end of the current-voltage conversion module, and the anode of the photodiode is grounded;
the output end of the current-voltage conversion module is electrically connected with the input end of the filtering module;
the output end of the filtering module is electrically connected with the input end of the detection module.
Optionally, the filtering module further includes: the circuit comprises a first resistor, a second resistor, a first capacitor and a second capacitor;
the first end of the first resistor is the input end of the filtering module, the output end of the first operational amplifier is the output end of the filtering module, and the second end of the first resistor is electrically connected with the positive input end of the first operational amplifier through the second resistor;
the first capacitor is electrically connected between the second end of the first resistor and the output end of the first operational amplifier;
the inverting input end of the first operational amplifier is electrically connected with the output end of the first operational amplifier, and the forward input end of the first operational amplifier is grounded through a second capacitor.
Optionally, the fluorescence detection circuit further comprises an isolation module;
the isolation module is electrically connected between the filtering module and the detection module.
Optionally, the isolation module includes: a second operational amplifier and a third resistor;
the output end of the filtering module is electrically connected with the forward input end of the second operational amplifier through a third resistor, the output end of the second operational amplifier is electrically connected with the input end of the detection module, and the reverse input end of the second operational amplifier is electrically connected with the output end of the second operational amplifier.
Optionally, the detection module includes: a processor;
the processor is electrically connected with the output end of the filtering module.
Optionally, the detection module further includes: an AD converter;
the AD converter is electrically connected between the filtering module and the processor.
Optionally, the current-voltage conversion module includes: a third operational amplifier and a fourth resistor;
the reverse input end of the third operational amplifier is the input end of the current-voltage conversion module, the output end of the third operational amplifier is the output end of the current-voltage conversion module, and the forward input end of the third operational amplifier is grounded;
the fourth resistor is electrically connected between the inverting input terminal of the third operational amplifier and the output terminal of the third operational amplifier.
Optionally, the current-voltage conversion module further includes: a third capacitor;
the third capacitor is connected in parallel with the fourth resistor.
Optionally, the current-voltage conversion module further includes: a fifth resistor;
the output end of the third operational amplifier is grounded through a fifth resistor.
The embodiment of the utility model provides a fluorescence check out test set is still provided, this equipment includes: casing, display screen and above-mentioned fluorescence detection circuitry.
The embodiment of the utility model provides a gather fluorescence signal and convert it into current signal through photodiode, convert current signal into voltage signal and enlarge through current-voltage conversion module, input to detection module behind the high frequency interference signal through among the filtering module filtering voltage signal to make detection module confirm fluorescent intensity according to the voltage signal who acquires. In the embodiment, the photodiode has the advantages of high detection precision, small size, low power consumption and the like, the practicability of the fluorescence detection circuit is improved, in addition, the filtering effect of the filtering module formed by the operational amplifier is better, and the accuracy of the detection result is improved. When the fluorescence detection circuit is used for detecting the content of oil in water, the content of oil in water can be determined according to the intensity of fluorescence, and the fluorescence detection circuit has good practicability.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural diagram of a fluorescence detection circuit according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of another fluorescence detection circuit provided in an embodiment of the present invention;
FIG. 3 is a schematic diagram of a fluorescence detection circuit according to an embodiment of the present invention;
fig. 4 is a schematic structural diagram of a fluorescence detection apparatus according to an embodiment of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention. It should be further noted that, for the convenience of description, only some of the structures related to the present invention are shown in the drawings, not all of the structures.
Fig. 1 is a schematic structural diagram of a fluorescence detection circuit according to an embodiment of the present invention, which can be used for detecting fluorescence intensity. For example, the circuit can detect the content of oil in water based on the principle that the fluorescence intensity is proportional to the content of oil in a water sample. Referring to fig. 1, the fluorescence detection circuit 10 includes: a photodiode 110, a current-voltage conversion module 120, a filtering module 130, and a detection module 140; the filtering module 130 includes a first operational amplifier (not shown); the cathode of the photodiode 110 is electrically connected to the input terminal of the current-voltage conversion module 120, and the anode of the photodiode 110 is grounded; the output end of the current-voltage conversion module 120 is electrically connected with the input end of the filtering module 130; the output terminal of the filtering module 130 is electrically connected to the input terminal of the detecting module 140.
By way of example, a photodiode of type S1337-66BQ may be used, which has a simple circuit structure, high fluorescence detection accuracy, a small circuit board area, and low power consumption, and can improve the practicability of the fluorescence detection circuit 10 compared to a photomultiplier tube. Taking the detection of the oil content in water as an example, when the oil in water is excited by the ultraviolet light irradiation to generate weak fluorescence, the fluorescence can be received by the photodiode 110 and generate a weak current signal, and the current intensity is proportional to the received fluorescence intensity. The fluorescence intensity can be determined by determining the current intensity.
However, the current signal is weak, which affects the accuracy of the detection result, and therefore, the current signal needs to be further processed. In this embodiment, the current signal is first converted into a voltage signal by the current-voltage conversion module 120 and then amplified, and then the high-frequency interference signal in the voltage signal is filtered by the filtering module 130, so as to improve the accuracy of the detection result. The accuracy of the detection result can be improved by converting the weak current signal into the amplified voltage signal, however, some high-frequency interference signals may be introduced into the circuit due to power supply, radiation, etc., and these high-frequency interference signals may affect the effective fluorescent signal after being amplified by the current-voltage conversion module 120, and therefore, need to be filtered.
Thus, after the weak current signal is processed as described above, the detection module 140 can determine the intensity of the fluorescence signal according to the magnitude of the voltage signal, so as to characterize the content of oil in water. It should be noted that the detection module 140 may be any device known to those skilled in the art with a data processing function, and the detection module 140 may determine the magnitude of the voltage signal and determine the intensity of the fluorescence signal according to the magnitude of the voltage signal. The following description will be made for the exemplary structure of the detection module 140, and will not be repeated herein.
It should be noted that fig. 1 does not show the first operational amplifier structure in the filtering module 130, and those skilled in the art can adopt any filtering circuit including an operational amplifier as the filtering module 130, and the embodiment of the present invention does not limit this, and will be exemplified in the following. The filter circuit formed by the operational amplifier and other devices can ensure good filtering effect, and further improve the accuracy of the detection result.
The embodiment of the utility model provides a gather fluorescence signal and convert it into current signal through photodiode, convert current signal into voltage signal and enlarge through current-voltage conversion module, input to detection module behind the high frequency interference signal through among the filtering module filtering voltage signal to make detection module confirm fluorescence signal's intensity according to the voltage signal who acquires. In the embodiment, the photodiode has the advantages of high detection precision, small size, low power consumption and the like, the practicability of the fluorescence detection circuit is improved, in addition, the filtering effect of the filtering module formed by the operational amplifier is better, and the accuracy of the detection result is improved. When the fluorescence detection circuit is used for detecting the content of oil in water, the content of oil in water can be determined according to the intensity of fluorescence, and the fluorescence detection circuit has good practicability.
Fig. 2 is a schematic structural diagram of another fluorescence detection circuit provided in the embodiment of the present invention, which is further optimized to improve circuit performance.
Referring to fig. 2, optionally, the fluorescence detection circuit 10 further includes an isolation module 150, and the isolation module 150 is electrically connected between the filtering module 130 and the detection module 140.
By providing the isolation module 150 between the filtering module 130 and the detection module 140, the influence between the filtering module 130 and the detection module 140 can be isolated, and the stability of the circuit can be improved. The specific structure of the isolation module 150 will be described in detail later, and will not be described herein.
Fig. 3 is a schematic structural diagram of another fluorescence detection circuit provided in an embodiment of the present invention, and on the basis of the above embodiment, a specific structure of the fluorescence detection circuit is exemplarily described below with reference to fig. 3.
Referring to fig. 3, optionally, the filter module 130 further includes a first resistor R1, a second resistor R2, a first capacitor C1, and a second capacitor C2, a first end of the first resistor R1 is an input end of the filter module 130, an output end of the first operational amplifier U1 is an output end of the filter module 130, a second end of the first resistor R1 is electrically connected to a positive input end of the first operational amplifier U1 through the second resistor R2, a first capacitor C1 is electrically connected between a second end of the first resistor R1 and an output end of the first operational amplifier U1, a negative input end of the first operational amplifier U1 is electrically connected to an output end of the first operational amplifier U1, and the positive input end of the first operational amplifier U1 is further grounded through the second capacitor C2.
In this embodiment, the first operational amplifier U1, the first resistor R1, the second resistor R2, the first capacitor C1 and the second capacitor C2 may form a second-order low-pass filter circuit to filter out high-frequency interference signals. By adjusting the resistance values of the first resistor R1 and the second resistor R2 and the capacitance values of the first capacitor C1 and the second capacitor C2, the low-pass frequency can be adjusted. The filter module 130 has a cutoff frequency of
For example, C1 ═ 4C2, R1 ═ R2 can be used, and then
Referring to fig. 3, optionally, the isolation module 150 includes a second operational amplifier U2 and a third resistor R3, the output terminal of the filtering module 130 is electrically connected to the positive input terminal of the second operational amplifier U2 through the third resistor R3, the output terminal of the second operational amplifier U2 is electrically connected to the input terminal of the detection module 140, and the negative input terminal of the second operational amplifier U2 is electrically connected to the output terminal of the second operational amplifier U2.
The second operational amplifier U2 can form a voltage follower, thereby playing the roles of isolation and buffering and improving the load capacity of the circuit. The third resistor R3 is provided to protect the input signal of the second operational amplifier U2.
Referring to fig. 3, optionally, the detection module 140 includes a processor 141, and the processor 141 is electrically connected to the output of the filtering module 130.
The processor 141 has a data processing function, and can calculate the intensity of the fluorescence signal according to the acquired voltage signal, thereby determining the content of oil in the water.
Since most of the existing processors can process digital signals, and the voltage signal is an analog signal, in order to improve the practicability of the fluorescence detection circuit 10, the detection module 140 further includes an AD converter 142, and the AD converter 142 is electrically connected between the filtering module 130 and the processor 141.
With this arrangement, the voltage analog signal can be converted into a digital signal by the AD converter 142, and the intensity of the fluorescence signal can be determined by the processor 141 from the acquired digital signal.
It should be noted that fig. 3 only shows a preferred circuit structure of the detection module 140 connected to the filtering module 130 through the isolation module 150, and does not show a specific circuit structure of the detection module 140 directly electrically connected to the filtering module 130.
With continued reference to fig. 3, optionally, the current-voltage conversion module 120 includes a third operational amplifier U3 and a fourth resistor R4, the inverting input terminal of the third operational amplifier U3 is the input terminal of the current-voltage conversion module 120, the output terminal of the third operational amplifier U3 is the output terminal of the current-voltage conversion module 120, the forward input terminal of the third operational amplifier U3 is grounded, and the fourth resistor R4 is electrically connected between the inverting input terminal of the third operational amplifier U3 and the output terminal of the third operational amplifier U3.
The weak current signal can be converted into an amplified voltage signal by the current-voltage conversion module 120, and the amplification factor can be changed by adjusting the resistance of the fourth resistor R4. The resistance of the fourth resistor R4 can be set by one skilled in the art according to the requirement, and is not limited herein.
With continued reference to fig. 3, further optionally, the current-voltage conversion module 120 further includes a third capacitor C3, and the third capacitor C3 is connected in parallel with the fourth resistor R4.
With such an arrangement, the third capacitor C3 and the fourth resistor R4 form a first-order low-pass filter, which also functions to filter out high-frequency interference signals.
With continued reference to fig. 3, further optionally, the current-voltage conversion module 120 further includes a fifth resistor R5, and the output terminal of the third operational amplifier U3 is grounded through the fifth resistor R5.
The fifth resistor R5 is provided to reliably ground the voltage signal output by the third operational amplifier U3, thereby performing impedance matching.
The embodiment of the utility model provides a still provide a fluorescence detection equipment, this equipment includes at least the utility model provides a fluorescence detection circuit, consequently the utility model provides a fluorescence detection equipment possesses the utility model discloses the beneficial effect of fluorescence detection circuit that the embodiment provides, the same part can refer to the above-mentioned description to fluorescence detection circuit, no longer gives unnecessary details here.
Fig. 4 is a schematic structural diagram of a fluorescence detection apparatus provided in an embodiment of the present invention, which can be used for detecting the oil content in water. Referring to fig. 4, the fluorescence detecting apparatus 20 includes: a housing 210, a display screen 220, and the fluorescence detection circuitry (not shown) described above.
Illustratively, the device may also include a number of function keys 230, such as a power key or the like. The function button 230 and the display screen 220 are electrically connected to the fluorescence detection circuit inside the housing 210, and are respectively used for operation of the fluorescence detection device and display of the detection result.
It should be noted that the foregoing is only a preferred embodiment of the present invention and the technical principles applied. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail with reference to the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the scope of the present invention.
Claims (10)
1. A fluorescence detection circuit, comprising: the device comprises a photodiode, a current-voltage conversion module, a filtering module and a detection module; the filtering module comprises a first operational amplifier;
the cathode of the photodiode is electrically connected with the input end of the current-voltage conversion module, and the anode of the photodiode is grounded;
the output end of the current-voltage conversion module is electrically connected with the input end of the filtering module;
and the output end of the filtering module is electrically connected with the input end of the detection module.
2. The fluorescence detection circuit of claim 1, wherein the filtering module further comprises: the circuit comprises a first resistor, a second resistor, a first capacitor and a second capacitor;
the first end of the first resistor is the input end of the filtering module, the output end of the first operational amplifier is the output end of the filtering module, and the second end of the first resistor is electrically connected with the positive input end of the first operational amplifier through the second resistor;
the first capacitor is electrically connected between the second end of the first resistor and the output end of the first operational amplifier;
the reverse input end of the first operational amplifier is electrically connected with the output end of the first operational amplifier, and the forward input end of the first operational amplifier is grounded through the second capacitor.
3. The fluorescence detection circuit of claim 1, further comprising an isolation module;
the isolation module is electrically connected between the filtering module and the detection module.
4. The fluorescence detection circuit of claim 3, wherein the isolation module comprises: a second operational amplifier and a third resistor;
the output end of the filtering module is electrically connected with the forward input end of the second operational amplifier through the third resistor, the output end of the second operational amplifier is electrically connected with the input end of the detection module, and the reverse input end of the second operational amplifier is electrically connected with the output end of the second operational amplifier.
5. The fluorescence detection circuit of claim 1, wherein the detection module comprises: a processor;
the processor is electrically connected with the output end of the filtering module.
6. The fluorescence detection circuit of claim 5, wherein the detection module further comprises: an AD converter;
the AD converter is electrically connected between the filtering module and the processor.
7. The fluorescence detection circuit of claim 1, wherein the current-to-voltage conversion module comprises: a third operational amplifier and a fourth resistor;
the reverse input end of the third operational amplifier is the input end of the current-voltage conversion module, the output end of the third operational amplifier is the output end of the current-voltage conversion module, and the positive input end of the third operational amplifier is grounded;
the fourth resistor is electrically connected between the inverting input terminal of the third operational amplifier and the output terminal of the third operational amplifier.
8. The fluorescence detection circuit of claim 7, wherein the current-to-voltage conversion module further comprises: a third capacitor;
the third capacitor is connected with the fourth resistor in parallel.
9. The fluorescence detection circuit of claim 7, wherein the current-to-voltage conversion module further comprises: a fifth resistor;
the output end of the third operational amplifier is grounded through the fifth resistor.
10. A fluorescence detection device, comprising: a housing, a display screen, and the fluorescence detection circuit of any of claims 1-9.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020349821.7U CN211741077U (en) | 2020-03-19 | 2020-03-19 | Fluorescence detection circuit and fluorescence detection equipment |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202020349821.7U CN211741077U (en) | 2020-03-19 | 2020-03-19 | Fluorescence detection circuit and fluorescence detection equipment |
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| CN211741077U true CN211741077U (en) | 2020-10-23 |
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| CN202020349821.7U Active CN211741077U (en) | 2020-03-19 | 2020-03-19 | Fluorescence detection circuit and fluorescence detection equipment |
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