CN211734363U - Reaction well, perforated plate and culture bed suitable for cell culture and biochemical reaction - Google Patents

Abstract

The utility model relates to the field of cell biology and biochemistry, in particular to a reaction well, a porous plate and a culture bed which are suitable for cell culture and biochemical reaction, wherein the reaction well comprises hydrophilic cotton and a holding well body, the hydrophilic cotton is used for implanting microorganisms and keeping absorbed culture solution for the growth of the microorganisms, and has a microporous structure for the adsorption of the microorganisms; the accommodating well body is provided with an accommodating cavity, and the hydrophilic cotton is fixed in the accommodating cavity; the utility model discloses a be provided with aforementioned reaction well on the perforated plate, the culture bed includes perforated plate, first baffle, solution diffusion layer, second baffle, microorganism detection portion and base plate. The utility model has the advantages of simple structure, be convenient for operation and detection cycle are short.

Description

Reaction well, perforated plate and culture bed suitable for cell culture and biochemical reaction

Technical Field

The utility model relates to a cell biology and biochemistry field are particularly a reaction well, perforated plate and culture bed suitable for cell culture and biochemical reaction.

Background

The existing biochemical reaction wells are hollow structures with only one opening at the top, and the multi-well plates are generally used for disposable articles in biological, biomedical and biochemical laboratories. The material channels in the perforated plate have different volume capacities, good shape, colour and material. The selection of a particular multi-well plate depends on the experiment or the procedure performed: certain procedures require specific pore shapes, volumes, or materials.

Common biochemical techniques and biochemical reactions are carried out in liquid form. The liquid medium is a nutrient source and a suitable environment for microorganisms or biochemical reactions. Therefore, when injecting a liquid into a conventional reaction well, it is necessary to deposit/store the medium from an opening at the top and then the liquid covers the bottom of the reaction well. The liquid is easily spilled and/or evaporated, causing unnecessary interference with the biochemical processes in the reaction mixture. To prevent leakage, conventional reaction wells are often not completely filled with reaction mixture. Conventional reaction wells must also be maintained in an orientation to prevent the flow of fluids from the reaction well. In addition, conventional reaction wells may be closed with a lid or sealed with a seal to prevent loss due to liquid leakage. This also prevents contamination from entering the reaction well. However, evaporation control is still very difficult, and when conventional reaction wells are covered with a cover or seal, it is not uncommon for droplets of liquid evaporated from the reaction mixture to settle on the seal or seal in the form of tiny droplets. In this case, the reaction mixture is efficiently reduced, which adversely affects the biochemical reactions occurring in the reaction mixture.

SUMMERY OF THE UTILITY MODEL

In order to overcome the defects existing in the prior art, the utility model provides a method for detecting the activity of microorganisms in reaction wells, perforated plates, culture beds and solutions, which has the advantages of simple structure, convenient operation and ideal biochemical reaction effect and is suitable for cell culture and biochemical reaction.

In order to solve the technical problem, the utility model adopts the following technical scheme:

the utility model relates to a reaction well suitable for cell culture and biochemical reaction, which comprises a hydrophilic cotton and a holding well body; the hydrophilic cotton is used for implanting microorganisms and maintaining absorbed culture solution for the growth of the microorganisms, and has a micropore structure for the adsorption of the microorganisms, and the pore diameter of the micropore is 0.05-50 microns; the accommodating well body is provided with an accommodating cavity, and the hydrophilic cotton is embedded in the accommodating cavity;

as a further improvement, the accommodating cavity is communicated up and down, part of the hydrophilic cotton is embedded in the accommodating cavity, and the other part of the hydrophilic cotton protrudes out of the accommodating cavity downwards.

As a further improvement, the accommodating cavity comprises an upper cavity and a lower cavity, wherein one part of the hydrophilic cotton is embedded in the lower cavity, and the other part of the hydrophilic cotton protrudes downwards out of the outer side of the lower cavity.

As a further improvement, the hydrophilic cotton is blocky cotton made of high water absorption polymer fibers.

The utility model also provides a perforated plate suitable for cell culture and biochemical reaction has the reaction well that a plurality of arrays were arranged, the aforementioned reaction well of reaction well.

As a further improvement, the porous plate is square and has 4-64 reaction wells.

The utility model further provides a culture bed suitable for cell culture and biochemical reaction, which comprises a porous plate and a base plate; the porous plate is mounted on the substrate and has the reaction well.

As a further improvement, the substrate is provided with a microorganism detection part for detecting a microorganism survival index, and the microorganism detection part is provided with resazurin (C12H7NO 4).

As a further improvement, the substrate is provided with an indication bulge corresponding to the reaction well, and the microorganism detection part is arranged on the indication bulge.

As a further improvement, a first clapboard is arranged between the substrate and the porous plate to separate the substrate and the porous plate, and after biochemical reaction is carried out in the reaction well, the first clapboard is drawn out to enable the microorganism detection part to be in contact with the reaction well so as to judge the biochemical reaction condition in the corresponding reaction well.

As a further improvement, a solution diffusion layer is arranged above the first partition plate to uniformly diffuse the microorganism culture solution to each reaction well, and the solution diffusion layer is a porous fabric sheet.

As a further improvement, a second clapboard is arranged between the porous plate and the solution diffusion layer to separate the porous plate and the solution diffusion layer, and the second clapboard is drawn out after the solution in the solution diffusion layer is uniformly diffused or is gradually dissolved in the solution diffusion process so as to enable the solution diffusion layer to be in contact with the reaction well and enable the reaction well to obtain the solution.

As a further improvement, one corner of the porous plate is provided with a liquid injection notch for injecting a microorganism culture solution, and part of the solution diffusion layer is exposed in the liquid injection notch.

As a further improvement, a shielding cover is arranged above the porous plate to prevent the solution in the hydrophilic cotton from volatilizing.

The utility model discloses a detection method of microbial activity in solution is applied to aforementioned a culture bed who is applicable to cell culture and biochemical reaction, its characterized in that, including following step:

s01, adding a solution to be detected into the solution diffusion layer, and standing for t1 to enable the solution to be detected to be uniformly distributed on the solution diffusion layer;

s02, drawing out the second partition plate, and standing for t2 to enable the hydrophilic cotton implanted with the microorganisms to absorb the solution to be tested from the solution diffusion layer for the microorganisms to grow;

s03, drawing out the first partition plate, and standing for t3 to enable microorganisms in the hydrophilic cotton to react with resazurin (C12H7NO4) on the microorganism detection part;

s04, checking the color change of the Resazurin (C12H7NO4) to judge the microbial activity index in the solution to be detected.

As a further improvement, the standing time t1 is 5-10 minutes, the standing time t2 is 30-210 minutes, and the standing time t3 is 5-45 minutes.

Owing to adopted above technical scheme, the utility model discloses following technological effect has been produced:

the utility model is used for reaction well, perforated plate and the culture bed that the microorganism was cultivateed, the structure is comparatively simple, adopts with the hydrophilic cotton cost of little biocompatibility lower, the utility model discloses simple operation, detection cycle are short, the reproducibility is good, small, light in weight, transportation and convenient to use detect when can satisfying big batch sample with low costs, improve work efficiency.

The utility model provides a little biological activity detection method in solution can realize the little biological activity who detects solution fast, if solution has the bacterinertness, then explains to contain certain or some antibiotic in this solution, and is corresponding, and this solution has the inhibitory action to the growth of certain or some microorganism, consequently, for the antibiotic kind that contains in detecting this solution, then implant different microorganism in the reaction well can. In practical tests, the solution can be milk, beverage and the like, particularly milk, and can quickly judge whether antibiotics are contained or not, so that the solution is more beneficial to the health of people and can also be used for industrial application.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art are briefly described as follows:

FIG. 1 is an exploded view of a reaction well according to a first embodiment of the present invention.

Fig. 2 is a structural view of a multi-well plate according to a first embodiment of the present invention.

Fig. 3 is a structural view of the other side of the porous plate of fig. 2.

Fig. 4 is a sectional structure view of the perforated plate in fig. 2.

FIG. 5 is an exploded view of a culture bed according to the first embodiment of the present invention.

Fig. 6 is a structural view of a base according to a first embodiment of the present invention.

FIG. 7 is an exploded view of a culture bed according to another embodiment of the present invention.

Fig. 8 is a schematic diagram of a detection method according to a first embodiment of the present invention.

Description of reference numerals:

1. a reaction well; 101. hydrophilic cotton; 102. accommodating the well body; 1021. an upper chamber; 1022. a lower chamber; 1023. a step portion; 2. a perforated plate; 201. a liquid injection notch; 3. a second separator; 4. a solution diffusion layer; 5. a first separator; 6. a microorganism detection unit; 7. a substrate; 701. an indication projection; 8. a shielding cover.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the present invention will be described in further detail with reference to the accompanying drawings. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Some embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Features in the embodiments described below may be combined with each other without conflict.

The first embodiment is as follows:

as shown in fig. 1 and 4, the reaction well 1 of the present invention comprises a hydrophilic cotton 101 and a containing well, wherein the hydrophilic cotton 101 is used for implanting microorganisms and maintaining absorbed culture solution for microorganism growth, and has a microporous structure for microorganism adsorption, the pore size of the microporous structure is in the range of 0.05 μm to 50 μm, preferably 0.5 to 25 μm, the pore size of the microporous structure is not a fixed value, but is different, but the minimum pore size is not less than 0.05 μm, and the maximum pore size is not more than 50 μm. The accommodating well 102 is provided with an accommodating cavity, and the hydrophilic cotton 101 is embedded in the accommodating cavity. In this example, nutrients and enzymes were added to the culture solution to promote the growth of the corresponding microorganisms. The containing cavity is communicated up and down, part of the hydrophilic cotton 101 is embedded in the containing cavity, and the other part of the hydrophilic cotton protrudes out of the containing cavity. The accommodating cavity comprises an upper cavity 1021 and a lower cavity 1022, wherein the upper cavity 1021 is circular, the lower cavity 1022 is square (or rectangular), part of the hydrophilic cotton 101 is embedded in the lower cavity 1022, the other part of the hydrophilic cotton protrudes downwards to the outside of the lower cavity 1022, a stepped part 1023 is arranged in the lower cavity 1022, and the stepped part 1023 protrudes from the side wall of the lower cavity 102 to clamp the hydrophilic surface 101 so as to keep the hydrophilic surface at a proper position. The diameter of the upper chamber 1021 is smaller than the width (side length of the square) of the lower chamber 1022, the block-shaped cotton made of the hydrophilic cotton 101 is embedded in the lower chamber 1022, and the upper chamber 1021 can be used for injecting a culture solution or implanting microorganism cells. In other embodiments, the accommodating cavity may have only one communicating chamber, and the hydrophilic cotton may be embedded in the accommodating cavity. In addition, the top of the accommodating cavity also can be provided with no opening, and the accommodating cavity with the structure needs to pre-plant microorganisms in the hydrophilic cotton when in operation and then install the hydrophilic cotton in the accommodating cavity. In summary, the accommodation chamber may have various structures as necessary to solve the technical problems of the present invention, which do not require creative efforts.

In the present embodiment, the hydrophilic cotton 101 is block cotton made of hydrophilic fibers. The hydrophilic fiber is a common oil filter felt, the felt is made of one or more polymer fibers, and comprises polymer fibers made of polyether, polyethylene or polypropylene, the material has high hydrophilicity and compatibility with microorganisms, cannot inhibit the growth of the microorganisms, and provides a good carrier for the growth of the microorganisms. The pore diameter of the oil filter felt is in the micron range, the pore diameter of the micropores is 0.05-50 microns, and the pore diameter is preferably 0.5-25 microns. In the prior art the application is to filter oils. The use of such filter felt polymer fibers as reaction wells for biochemical reactions is new and new uses for the filter felt have been developed and are unexpected in the art.

In other embodiments, the hydrophilic cotton 101 is a block cotton made of super absorbent polymer fibers. The high water absorption fiber comprises natural fiber and two artificial fibers (viscose fiber and acetate fiber), and mainly comprises cellulose fiber, polyacrylic acid (salt) fiber, polyvinyl alcohol fiber, vinyl acetate copolymer fiber, polyurethane fiber, polyethylene oxide fiber or starch graft copolymer fiber. Similarly, the hydrophilic cotton 101 is biocompatible, i.e., does not inhibit the growth of microorganisms, and is also generally referred to as a cell-compatible fiber.

It should be noted that, to the selection of hydrophilic cotton 101 in the utility model, the ordinary skilled person in the art can be according to the utility model provides a guide select in current hydrophilic fiber can, certainly also can select in the cellucotton of future new production, as long as this fibre satisfies stronger hydrophilicity and little biocompatibility, and have appropriate aperture and can allow cell position relatively fixed, and nutrient or antibiotic can be absorbed easily and evenly distributed can, hydrophilicity is stronger indicates that this cellucotton can absorb the solution better and can avoid the volatilization of solution, little biocompatibility is better also indicates that it does not have the bacterinertness, also be favorable to the growth and reproduction of microorganism more, thereby improve experimental degree of accuracy.

In the present embodiment, the hydrophilic cotton 101 refers to a cotton-like object formed by interlacing hydrophilic fibers to facilitate absorption of a culture solution. To facilitate mounting and fixing, the hydrophilic cotton 101 is formed in a block shape and embedded in the lower chamber 1022 in this embodiment, while the hydrophilic cotton 101 has a portion protruding outside the lower chamber 1022, which is in contact with the solution diffusion layer 4 to absorb the culture solution. Since the hydrophilic cotton 101 has high water absorption, it can keep the absorbed solution from volatilizing quickly, thereby facilitating the growth of microorganisms.

Microorganisms, including bacteria, viruses, fungi, and some small protists, microscopic algae, are closely related to human life, although the individual is tiny, and the microorganisms cover many kinds of beneficial or harmful microorganisms, and widely relate to the fields of food, medicine, agriculture and industry, environmental protection, and science and technology. The microorganism mainly includes cell-like organisms, such as box-type Fischer-Tropsch cell microorganism, wherein the non-cell organism is composed of a few components such as nucleic acid and protein, but the survival of the non-cell organism is dependent on living cells such as virus, but the microorganism of the present invention mainly refers to cell-like organisms such as bacteria, fungi, etc.

Specifically, the receiving well 102 in this embodiment may have other structures, such as a circular shape in both the upper chamber and the lower chamber, but the central axes of the two chambers are staggered. It is also possible to provide a structure with a bottomed opening hole and further enlarge the size of the bottom portion for fixing the hydrophilic cotton 101. In summary, the accommodating well 102 of the present invention is used for installing or placing the hydrophilic cotton 101, and one of ordinary skill in the art can set the accommodating well 102 with a corresponding structure according to the shape of the hydrophilic cotton 101 or the specific installation requirement, and the size or shape of the accommodating well 102 can be changed in the same way, which should be within the protection scope of the present invention.

As shown in fig. 2, fig. 3 and fig. 4, the multi-well plate 2 suitable for cell culture and biochemical reaction of the present invention has a plurality of reaction wells 1 arranged in an array, the multi-well plate 2 is square and has 4 to 64 reaction wells 1, in this embodiment, there are 36 reaction wells 1 on the multi-well plate 2, the number of the reaction wells 1 can also be 4, 16, 25, 49, 64, etc., the larger the number of the reaction wells 1, the more test samples mean that more bacterial strains can be tested at a time, or more repeated test results, which results in more extensive and accurate results. The porous plate 2 is formed by injection molding of plastic such as polylactic acid and polystyrene (in other embodiments, 3D printing can be used), and the plastic material should not have a bacteria inhibiting effect to avoid adverse effects on the growth of microorganisms in the hydrophilic cotton 101. The perforated plate 2 in this embodiment is a square block of 45 × 5.5mm (length × width × height), and the change in the shape of the holes does not affect the solution of the technical problem.

As shown in FIGS. 5 and 6, the culture bed of the present invention for cell culture and biochemical reaction comprises a multi-well plate 2 and a base 7; the porous plate 2 is mounted on the base 7, and the porous plate 2 is provided with a reaction well 1. A microorganism detecting part 6 for detecting the survival index of the microorganism is arranged on the base 7 corresponding to the position of the reaction well 1, the microorganism detecting part 6 is provided with resazurin (C12H7NO4), and the resazurin is directly coated on the microorganism detecting part 6. The base 7 is provided with an indicating protrusion 701 corresponding to the reaction well 1, and the indicating protrusion 701 is integrally formed on the base 7. The microorganism detection part 6 is a translucent film adhered to the inside of the base 7 and covering the indication protrusion 701. The indicating protrusion 701 functions to bring the microorganism detecting part 6 into point-to-point contact with the hydrophilic cotton 101, not into surface contact, so that the resazurin (C12H7NO4) can accurately show the growth of microorganisms in each hydrophilic cotton 101 without mixing, and particularly when the solution diffusion layer 4 is present therebetween, the indicating protrusion 701 can lift up the solution diffusion layer 4 to prevent the solution diffusion layer 4 from absorbing the resazurin and diffusing the resazurin to the periphery when the first partition plate 5 is drawn out, thereby reducing the detection accuracy. And a second partition plate 3 is arranged between the solution diffusion layer 4 and the porous plate 2 to separate the solution diffusion layer and the porous plate 2, the second partition plate 3 can be inserted into or pulled out from the second partition plate 3, in practical use, the second partition plate 3 is inserted into or pulled out from the space between the solution diffusion layer 4 and the porous plate 2 in the production process, after the solution is uniformly diffused, the solution is pulled out from the space between the solution diffusion layer 4 and the porous plate 2 so that the solution diffusion layer 4 is contacted with the reaction well 1, and the reaction well 1 obtains the solution from the solution diffusion layer 4 for culturing microorganisms. The second partition plate 3 serves to prevent the solution diffusion layer 4 from contacting the reaction wells 1 before the injected solution is not uniformly diffused to prevent the reaction wells near the solution injection port from receiving more solution, while the reaction wells far from the solution injection port do not receive solution or receive less solution. The second partition 3 is a PC transparent sheet in this embodiment, but may be another plastic sheet. In addition, in other embodiments, the second separator 3 may also use a soluble paper that can be gradually dissolved during the solution diffusion process. The first partition plate 5 is interposed between the solution diffusion layer 4 and the microorganism detection section 6, and the first partition plate 5 is interposed between the solution diffusion layer 4 and the microorganism detection section 6 during the actual use, and is drawn out from between the solution diffusion layer 4 and the microorganism detection section 6 after the culture of the microorganisms is completed so that resazurin (C12H7NO4) in the microorganism detection section 6 reacts with the solution, since resazurin (C12H7NO4) is a ph indicator (ph3.8 (orange) to 6.5 (deep purple)) and is also an oxidation-reduction indicator, since the ph of the solution can be changed during the growth of the microorganisms and the change in color of resazurin (C12H7NO4) clearly reflects the growth of pink microorganisms, in this embodiment, resazurin is blue in the initial state, and after the reaction, resazurin changes to a color, the color change of the solution indicates that the cells in the solution breathe and is therefore an indirect indication of the number of cells. The shielding cover 8 is arranged above the porous plate 2 to prevent the solution in the hydrophilic cotton 101 from volatilizing, the shielding cover 8 can form a seal at the wellhead of the reaction well 1, when the solution volatilizes in the hydrophilic cotton 101, the liquid drops liquefied on the shielding cover 8 can be reabsorbed by the hydrophilic cotton 101 due to the effect of the shielding cover 8, so that an evaporation-liquefaction-absorption cycle is generated, and the biochemical reaction inside the hydrophilic cotton 101 is stabilized. In the present embodiment, the shielding cover 8 is made of a transparent material PC, but may be made of a non-transparent material.

In another embodiment, the solution diffusion layer 4 and the first separator 5 may be integrated, that is, the solution diffusion layer 4 may be attached to the first separator 5, and the solution diffusion layer 4 may be simultaneously extracted when the first separator 5 is extracted. In addition, resazurin can also be injected into the hydrophilic cotton 101 directly from above the reaction well 1.

As shown in fig. 2 to 4, in order to facilitate the injection of the solution into the solution diffusion layer 4, one corner of the porous plate 2 and one corner of the first partition plate 5 are each provided with an injection notch 201, and a part of the solution diffusion layer 4 is exposed in the injection notch 201.

In this embodiment, the solution diffusion layer 4 can uniformly diffuse the injected solution below the reaction well 1 for absorption by the hydrophilic cotton 101, and is in a thin film shape. The material of the solution diffusion layer 4 is the same as or similar to the material of the hydrophilic cotton 101, and both have hydrophilicity and microbial compatibility, but should not have antibacterial property and solution filterability, so as to avoid inhibiting the growth of microorganisms and also avoid filtering out components which are beneficial to the growth of microorganisms in the solution. However, in order to facilitate the hydrophilic cotton 101 to absorb the culture solution of the solution diffusion layer 4 more sufficiently, a hydrophilic fiber material having a water absorption performance not higher than that of the hydrophilic cotton 101 may be selected as the material of the solution diffusion layer 4. It should be noted that the first partition plate 5 and the second partition plate 3 are made of a non-water-absorbing plastic material, such as polylactic acid, polystyrene, etc., and in this embodiment, a PC plastic sheet is used, and is pre-assembled in the culture bed. After the injected solution is uniformly diffused on the solution diffusion plate, the second partition plate 3 is drawn out, and after the growth of the microorganisms is completed, the first partition plate 5 is drawn out.

Specifically, since the first partition plate 5 functions to form a partition between the solution diffusion layer 4 and the microorganism detection section 6 to prevent the microorganism detection section 6 from reacting with the solution prematurely, in fact, if the solution diffusion layer 4 is not provided, it is not necessary to provide the second partition plate 3, and only the first partition plate 5 needs to be provided. When the solution diffusion layer 4 is not provided, the solution may be directly dropped from the upper portion of the absorption well, and after the biochemical reaction of the microorganism is completed, the first partition plate 5 may be drawn out to bring the hydrophilic cotton 101 into contact with the microorganism detection part 6. In addition, as shown in FIG. 7, the microorganism detection part 6 of the present invention may be divided into a plurality of small sheets, and the sheet containing Resazurin (C12H7NO4) is directly attached to the indication protrusion 701 or the Resazurin is deposited on the indication protrusion 701, and the indication protrusions 701 are bosses directly injection-molded on the base 7, and the number of the bosses is the same as the number of the reaction wells 1.

As shown in fig. 5 and 8, the method for detecting the activity of microorganisms in solution in the present embodiment is applied to the culture bed suitable for cell culture and biochemical reaction, and comprises the following steps:

s01, injecting a solution to be detected into the solution diffusion layer 4, and standing for t1 to enable the solution to be detected to be uniformly distributed on the solution diffusion layer 4;

s02, the second partition plate 3 is pulled out, and the standing time is t2, so that the hydrophilic cotton 101 implanted with the microorganisms absorbs the solution to be tested from the solution diffusion layer 4 for the growth of the microorganisms;

s03, the first partition board 5 is drawn out and is kept still for a time t3, so that microorganisms in the hydrophilic cotton 101 react with the resazurin (C12H7NO4) on the microorganism detection part 6,

s04, checking the color change of the Resazurin (C12H7NO4) to judge the microbial activity index of the solution to be detected.

The standing time t1 is 5 to 10 minutes, the standing time t2 is 30 to 210 minutes, and the standing time t3 is 5 to 45 minutes. In this example, the standing time t1 was 8 minutes, t2 was 120 minutes, and t3 was 20 minutes. It should be noted that the setting of the rest times t1, t2 and t3 is not limited to the above time ranges, and can be adjusted according to the actual experimental requirements, the length of the rest time depends on the microorganism used, the solution or the expected antibiotic type tested, for example, the rest time t1 is to allow the solution to spread evenly on the solution spreading layer 4 without evaporating.

As shown in fig. 2, the porous plate 2 is a porous plate 2 made of hardened plastic or resin, and the hardened plastic may include polylactic acid and polystyrene.

In addition, it should be noted that the utility model still needs the conventional means to assist in the using process, but the effect is greatly improved compared with the prior art. For example, droplets on the internal seals of a conventional reaction well 1 do not spontaneously return to the reaction mixture.

The utility model discloses an operating principle will be at first hydrophilic cotton 101 is installed and is made absorption well 1 in the holding well body on perforated plate 2, then further assembles into the culture bed with first baffle 5, solution diffusion layer 4, second baffle 3, microorganism detection portion 6 and base 7 in proper order again, implants the cell in hydrophilic cotton 101 again, also can just implant hydrophilic cotton 101 with the cell between the assembly in, it is internal to embed it into the holding well again, can also implant the microbial cell again after hydrophilic cotton 101 accomplishes the absorption culture solution. After assembling the microorganism culture bed, the culture solution is transferred into the hydrophilic cotton 101 through the solution diffusion layer 4 by a pipette, then the hydrophilic cotton 101 is positioned in the reaction well 1, the whole reaction well 1 device is placed in a proper environment for reaction, in the process, as the hydrophilic cotton 101 locks the culture solution in the reaction well, any steps can be performed in any direction in the whole process, such as horizontal, vertical or upside down, an operator can completely freely orient, hatch and analyze, and a visual result can be obtained by capturing images.

The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications, additions and substitutions for the specific embodiments described herein may be made by those skilled in the art without departing from the spirit of the invention or exceeding the scope of the invention as defined in the accompanying claims.

Although 1. reaction well is used more herein; 101. hydrophilic cotton; 102. an accommodating cavity; 1021. an upper chamber; 1022. a lower chamber; 1023. a step portion; 2. a perforated plate; 201. a liquid injection notch; 3. a second separator; 4. a solution diffusion layer; 5. a first separator; 6. a microorganism detection unit; 7. a base; 701. an indication projection; 8. masking the lid, etc., but does not exclude the possibility of using other terms. These terms are used merely to more conveniently describe and explain the nature of the present invention; they are to be construed in a manner that is inconsistent with the spirit of the invention.

Claims (14)

1. A reaction well suitable for cell culture and biochemical reaction is characterized by comprising hydrophilic cotton and a holding well body;

the hydrophilic cotton is used for implanting microorganisms and maintaining absorbed culture solution for the growth of the microorganisms, and has a micropore structure for the adsorption of the microorganisms, and the pore diameter of the micropore is 0.05-50 microns;

the holding well is internally provided with a holding cavity, and the hydrophilic cotton is embedded in the holding cavity.

2. The reaction well according to claim 1, wherein the receiving cavity is vertically through, a part of the hydrophilic cotton is embedded in the receiving cavity, and another part of the hydrophilic cotton protrudes downward out of the receiving cavity.

3. The reaction well for cell culture and biochemical reactions according to claim 1 or 2, wherein the accommodating cavity comprises an upper cavity and a lower cavity, and a part of the hydrophilic cotton is embedded in the lower cavity, and the other part of the hydrophilic cotton protrudes downwards outside the lower cavity.

4. The reaction well for cell culture and biochemical reactions according to claim 1, wherein the hydrophilic cotton is bulk superabsorbent polymer cellucotton.

5. A multiwell plate suitable for cell culture and biochemical reactions, having a plurality of reaction wells arranged in an array, wherein the reaction wells are according to any one of claims 1 to 4.

6. The multi-well plate for cell culture and biochemical reaction according to claim 5, wherein the multi-well plate is square and has 4 to 64 reaction wells.

7. A culture bed suitable for cell culture and biochemical reaction is characterized by comprising a porous plate and a base plate; the perforated plate is mounted on a base plate and has a reaction well according to any one of claims 1 to 4.

8. The culture bed of claim 7, wherein the substrate is provided with a microorganism detection part for detecting a microorganism survival index, and the microorganism detection part comprises resazurin (C12H7NO 4).

9. A culture bed suitable for cell culture and biochemical reactions according to claim 8, wherein the substrate is provided with an indication protrusion corresponding to the reaction well, and the microorganism detection part is provided on the indication protrusion.

10. The culture bed for cell culture and biochemical reaction according to claim 8 or 9, wherein a first partition is disposed between the substrate and the porous plate to separate the substrate from the porous plate, and after the biochemical reaction is completed in the reaction well, the first partition is drawn out to contact the microorganism detection part with the reaction well to determine the biochemical reaction in the corresponding reaction well.

11. The culture bed for cell culture and biochemical reactions according to claim 10, wherein a solution diffusion layer is further disposed above the first partition plate to uniformly diffuse the microorganism culture solution to each reaction well position, and the solution diffusion layer is a porous fabric sheet.

12. The culture bed for cell culture and biochemical reactions according to claim 11, wherein a second partition is disposed between the porous plate and the solution diffusion layer to separate them, and the second partition is drawn out after the solution in the solution diffusion layer is uniformly diffused or gradually dissolved during the solution diffusion process to contact the solution diffusion layer with the reaction well so that the reaction well receives the solution.

13. A culture bed suitable for cell culture and biochemical reactions according to claim 11 or 12, wherein a corner of the multi-well plate has a filling notch for filling with a microbial culture solution, and a portion of the solution diffusion layer is exposed in the filling notch.

14. A culture bed suitable for cell culture and biochemical reactions according to claim 11 or 12, wherein a shielding cover is arranged above the porous plate to prevent the solution in the hydrophilic cotton from volatilizing.

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