CN211672135U - Chip carrier for single sperm or very small amount of sperm - Google Patents
Chip carrier for single sperm or very small amount of sperm Download PDFInfo
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- CN211672135U CN211672135U CN201921236047.2U CN201921236047U CN211672135U CN 211672135 U CN211672135 U CN 211672135U CN 201921236047 U CN201921236047 U CN 201921236047U CN 211672135 U CN211672135 U CN 211672135U
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Abstract
The utility model discloses a chip carrier for single sperm or few sperm, indicate axle and microarray including chip carrier, X, Y, the chip carrier comprises coating, poly material layer and the specific antibody of absorption on the coating. The polymer material layer is positioned at the bottommost part of the chip carrier, the upper surface of the polymer material layer is attached to the lower surface of the coating, and the coating is provided with a plurality of microarrays. The utility model has the advantages that: the utility model discloses a put the single sperm of choosing under the microscope on the microarray on the chip carrier, then put into with the chip and freeze and carry out the conventional storage that freezes and can accomplish single sperm in depositing the pipe, realize the effective storage of single sperm or few sperm, avoid cross contamination, can realize the accurate access of sperm again, maneuverability is very strong, solves few sperm patient sperm and freezes the demand of depositing.
Description
The technical field is as follows:
the utility model belongs to the technical field of the chip carrier, in particular to a chip carrier that is used for single sperm or few sperm.
Background art:
at present, the number of families with infertility worldwide accounts for about 40% -50% due to male reasons, wherein azoospermia affects 1% of male population worldwide, and is a significant problem for human survival and multiplication. According to the research data of an international research group, the number of male sperms in the countries in progress is also reduced from 1973 to 2011, including north america, australia, new zealand and parts of european countries, by 52%, from about 9900 million sperms per ml to 4700 million sperms per ml, at a slower rate than in developed countries. This report has raised worldwide concerns and therefore the preservation of male fertility will become a major public health problem in the near future. Male infertility can be classified into primary infertility and secondary infertility. The research shows that genetic factors are important influencing factors, and the genetic mutation or the genetic deletion of methionine synthetase reductase gene, CLOCK gene and the like, SOHLH1 and the like are all reasons of primary male sterility. Secondary male infertility is mainly caused by environmental pollution, bad living habits, harmful exposure and the like, and with the continuous increase of environmental pollution, age and living pressure, more and more males suffer from abnormal sperm quality and incapability of bearing. With the release of the national second birth policy, the nation encourages people to breed more offspring, and the need of people for breeding is more and more urgent. In summary, the incidence of male infertility is gradually increasing and attracting human attention, and fertility preservation is an urgent clinical need. For patients with oligoasthenospermia, a small amount of high-quality sperms obtained by manual screening are very precious, and for patients with azoospermia, a very small amount of sperms obtained by puncturing or operation are more hoped for the sperm to grow, so the preservation of the sperms is very important, and therefore, the oligospermia or poor sperm quality caused by environment, living habits or unknown reasons exist in clinic, many patients have fertility requirements, and an effective method for freezing and storing few sperms is urgently needed.
At present, due to the environment, living habits or poor sperm quality caused by unknown reasons, many patients have fertility requirements clinically, and an effective method for freezing and storing few sperms is urgently needed.
The utility model has the following contents:
the present invention is directed to provide a chip carrier for single sperm or very small amount of sperm in order to solve the above problems.
In order to solve the above problem, the utility model provides a technical scheme:
the utility model provides a storage method and chip carrier that is used for single sperm or few sperm, includes chip carrier, X instruction axle, Y instruction axle and microarray, the chip carrier comprises coating and poly material layer, the poly material layer is located the chip carrier bottommost, poly material layer upper surface laminating is in the lower surface of coating, be equipped with a plurality of microarrays on the coating.
Preferably, an X indicating shaft and a Y indicating shaft are respectively arranged on two adjacent sides of the coating, capital letters which are the same as the number of the microarray rows and are arranged in sequence are arranged on the Y indicating shaft, and lowercase letters which are the same as the number of the microarray rows and are arranged in sequence are arranged on the polymer material layer.
Preferably, the microarray is in the form of a specific antibody.
Preferably, the coating is one of polylysine, protamine and avidin biotin.
Preferably, the array number of the microarray is determined by the areas of the polymer material layer and the coating, and the spacing between two adjacent grooves in the microarray is less than 50 nm; alternatively, the microarray is randomly distributed on the coating.
Preferably, a gray mark line is arranged between two adjacent grooves in the microarray.
Preferably, the size of the chip carrier is less than or equal to 1mm x 1mm, and a pull wire is arranged at one corner of the chip carrier 1.
Preferably, the polymeric material in the polymeric material layer is selected from one of polystyrene, nitrocellulose, polyvinyl chloride and polyethylene.
The utility model has the advantages that:
the utility model discloses a put the microarray on the chip carrier with the single sperm of choosing under the microscope, then put into with the chip and freeze and deposit and carry out the conventional storage that can accomplish single or a small amount of sperm in managing, realize the effective storage of single sperm or few sperm, avoid cross contamination, can realize the accurate access of sperm again, maneuverability is very strong, solves few sperm patient sperm and freezes the demand of depositing.
Description of the drawings:
for ease of illustration, the invention is described in detail by the following detailed description and accompanying drawings.
Fig. 1 is a schematic structural view of the present invention;
fig. 2 is a schematic diagram of the X indicating axis and the Y indicating axis of the present invention.
In the figure: 1. a chip carrier; 2. coating; 3. a microarray; 4. a layer of polymeric material; 5. x indicates an axis; 6. y indicates an axis; 7. and (6) pulling the wire.
The specific implementation mode is as follows:
as shown in fig. 1-2, the following technical solutions are adopted in the present embodiment: a storage method for single sperms or very small amount of sperms and a chip carrier comprise a chip carrier 1, an X indicating shaft 5, a Y indicating shaft 6 and a microarray 3, wherein the chip carrier 1 is composed of a coating layer 2 and a polymer material layer 4, the polymer material layer 4 is positioned at the bottommost part of the chip carrier 1, the upper surface of the polymer material layer 4 is attached to the lower surface of the coating layer 2, and the coating layer 2 is provided with a plurality of microarrays 3.
Furthermore, an X indicating shaft 5 and a Y indicating shaft 6 are respectively arranged on two adjacent sides of the coating layer 2, capital letters which are the same as the number of columns of the microarray 3 and are arranged in sequence are arranged on the Y indicating shaft 6, lowercase letters which are the same as the number of rows of the microarray 3 and are arranged in sequence are arranged on the polymeric material layer 4, and the serial number of each single sperm is recorded in a coordinate mode.
Further, the microarray 3 is a specific antibody type microarray 3, and the sperm head is fixed on the microarray 3 by realizing precise recognition.
Further, the coating 2 is one of polylysine, protamine, and avidin biotin, which facilitates immobilization of specific antibodies of the microarray 3 on a polymeric material, and then adsorption of sperm using the antibodies.
Furthermore, the array number of the microarray 3 is determined by the areas of the polymer material layer 4 and the coating 2, and the distance between two adjacent grooves in the microarray 3 is less than 50 nm; or randomly distributed over the coating 2 to prevent cross-contamination.
Furthermore, a gray mark line is arranged between two adjacent grooves in the microarray 3, so that the coordinates can be observed and identified conveniently.
Furthermore, the size of the chip carrier 1 is smaller than or equal to 1mm x 1mm, and one corner of the chip carrier 1 is provided with a traction wire 7, so that the chip carrier 1 can be taken out conveniently.
Further, the polymeric material in the polymeric material layer 4 is selected from one of polystyrene, nitrocellulose, polyvinyl chloride and polyethylene.
Further, the specific antibodies on the microarray 3 are immobilized on the polymeric material layer 4 by the adsorption of the coating layer 2.
Specifically, the method comprises the following steps: when the storage device is used, single sperms picked under a microscope are placed on the microarray 3 on the chip carrier 1, and then the chip is placed in a freezing tube to be frozen conventionally, so that the storage of the single or a small amount of sperms can be completed. Wherein, the adjacent two sides of the coating 2 are respectively provided with an X indicating shaft 5 and a Y indicating shaft 6, the Y indicating shaft 6 is provided with capital letters which have the same number as the columns of the microarray 3 and are arranged in sequence, the polymeric material layer 4 is provided with lowercase letters which have the same number as the rows of the microarray 3 and are arranged in sequence, and the serial number of each single sperm is recorded in a coordinate mode; the microarray 3 is a specific antibody type microarray 3, and the head of the sperm is fixed on the microarray 3 by realizing the precise recognition function; the coating 2 is one of polylysine, protamine and avidin biotin, so that a specific antibody is conveniently fixed on the polymer material layer 4, and then the antibody is used for adsorbing sperms; the array number of the microarray 3 is determined by the areas of the polymer material layer 4 and the coating 2, and the distance between two adjacent grooves in the microarray 3 is less than 50nm, so that cross contamination is prevented; or randomly distributed over the coating 2. And gray identification lines are arranged between two adjacent grooves in the microarray 3, so that the coordinates can be observed and identified conveniently.
The basic principles and main features of the present invention and the advantages of the present invention have been shown and described above, and it should be understood by those skilled in the art that the present invention is not limited by the above embodiments, which are only illustrative, but also various changes and modifications may be made without departing from the spirit and scope of the present invention, which fall within the scope of the present invention as claimed, which is defined by the appended claims and their equivalents.
Claims (9)
1. A chip carrier for single or very small amount of sperm, comprising a chip carrier (1), an X indicating axis (5), a Y indicating axis (6) and a microarray (3), characterized in that: the chip carrier (1) is composed of a coating (2) and a polymer material layer (4), the polymer material layer (4) is located at the bottommost part of the chip carrier (1), the upper surface of the polymer material layer (4) is attached to the lower surface of the coating (2), and a plurality of microarrays (3) are arranged on the coating (2).
2. A chip carrier for use with single or minimal sperm cells as claimed in claim 1, wherein said chip carrier comprises: the adjacent both sides of coating (2) are equipped with X respectively and indicate axle (5) and Y and indicate axle (6), be equipped with on Y and indicate axle (6) and arrange the capital letter the same with microarray (3) row number and in proper order, be equipped with the lowercase letter the same with microarray (3) row number and in proper order on the polymerized material layer (4).
3. A chip carrier for use with single or minimal sperm cells as claimed in claim 1, wherein said chip carrier comprises: the microarray (3) is a specific antibody type microarray (3).
4. A chip carrier for use with single or minimal sperm cells as claimed in claim 1, wherein said chip carrier comprises: the coating (2) is one of polylysine, protamine and avidin biotin.
5. A chip carrier for use with single or minimal sperm cells as claimed in claim 1, wherein said chip carrier comprises: the array number of the microarray (3) is determined by the area of the polymer material layer (4) and the coating (2), and the distance between two adjacent grooves in the microarray (3) is less than 50 nm.
6. A chip carrier for use with single or minimal sperm cells as claimed in claim 1, wherein said chip carrier comprises: and gray marking lines are arranged between two adjacent grooves in the microarray (3).
7. A chip carrier for use with single or minimal sperm cells as claimed in claim 1, wherein said chip carrier comprises: the size of the chip carrier (1) is less than or equal to 1mm x 1mm, and one corner of the chip carrier (1) is provided with a pull wire (7).
8. A chip carrier for use with single or minimal sperm cells as claimed in claim 1, wherein said chip carrier comprises: the polymer material in the polymer material layer (4) is selected from one of polystyrene, nitrocellulose, polyvinyl chloride and polyethylene.
9. A chip carrier for use with single or minimal sperm cells as claimed in claim 1, wherein said chip carrier comprises: the specific antibody on the microarray (3) is fixed on the polymer material layer (4) through the adsorption of the coating (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921236047.2U CN211672135U (en) | 2019-08-01 | 2019-08-01 | Chip carrier for single sperm or very small amount of sperm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921236047.2U CN211672135U (en) | 2019-08-01 | 2019-08-01 | Chip carrier for single sperm or very small amount of sperm |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211672135U true CN211672135U (en) | 2020-10-16 |
Family
ID=72770043
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921236047.2U Active CN211672135U (en) | 2019-08-01 | 2019-08-01 | Chip carrier for single sperm or very small amount of sperm |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211672135U (en) |
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2019
- 2019-08-01 CN CN201921236047.2U patent/CN211672135U/en active Active
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