CN211645266U

CN211645266U - Efficient immobilized enzyme electromagnetic recovery enzymolysis tank

Info

Publication number: CN211645266U
Application number: CN202020091606.1U
Authority: CN
Inventors: 毛相朝; 孙建安; 孟莹; 薛长湖
Original assignee: Ocean University of China
Current assignee: Ocean University of China
Priority date: 2020-01-15
Filing date: 2020-01-15
Publication date: 2020-10-09
Anticipated expiration: 2030-01-15

Abstract

The utility model relates to the technical field of food, feed and medicine processing equipment, and discloses an efficient immobilized enzyme electromagnetic recovery enzymolysis tank, which comprises an enzymolysis tank body, an electromagnetic recovery device and a stirring device, wherein the enzymolysis tank body comprises a tank wall, a temperature probe and a spiral pipe, the front side of the tank wall is fixedly provided with the temperature probe, the temperature probe is contacted with the inside of the tank wall, the spiral pipe is fixedly connected on the inner wall of the tank wall, the spiral pipe is contacted with the temperature probe, the upper surface of the tank wall is fixedly connected with a feed inlet, the feed inlet is communicated with the inside of the tank wall, the upper surface of the tank wall is provided with a round hole, the inside of the enzymolysis tank body is provided with the electromagnetic recovery device, the electromagnetic recovery device is positioned at the bottom part of the enzymolysis tank body, the electromagnetic recovery device comprises a discharge hole, a first electromagnetic recovery device, the efficient immobilized enzyme electromagnetic recovery enzymolysis tank has the performances of reducing cost, uniformly stirring and the like.

Description

Efficient immobilized enzyme electromagnetic recovery enzymolysis tank

Technical Field

The utility model relates to a food, fodder, medicine processing equipment technical field specifically are an enzymolysis tank is retrieved to efficient immobilized enzyme electromagnetism.

Background

The enzymolysis conversion is a common processing mode of raw materials such as food, feed, medicine and the like, and is widely applied due to the characteristics of mild reaction conditions, high efficiency of reaction process, high catalytic efficiency and the like, but a large amount of enzyme preparations are needed during the enzymolysis conversion, and the high cost of the enzyme preparations is a bottleneck problem of industrialization of the enzyme catalysis process, so that the immobilized enzyme is a feasible method for solving the problem, the stability of the enzyme can be enhanced after the enzyme is immobilized, and the enzyme can be recycled due to the increase of the volume or other characteristics, so that the cost of the enzyme preparation is greatly reduced, but how to efficiently recycle the immobilized enzyme in the enzyme catalysis process, and further efficiently recycle the immobilized enzyme, and the problem which needs to be solved for realizing the efficient catalysis of the immobilized enzyme is solved.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

To prior art not enough, the utility model provides an enzymolysis tank is retrieved to efficient immobilized enzyme electromagnetism possesses advantages such as reduce cost and stirring, has solved the problem that the enzyme preparation is with high costs.

(II) technical scheme

In order to achieve the above object, the utility model provides a following technical scheme: an efficient immobilized enzyme electromagnetic recovery enzymolysis tank comprises an enzymolysis tank body, an electromagnetic recovery device and a stirring device, wherein the enzymolysis tank body comprises a tank wall, a temperature probe and a spiral pipe, the temperature probe is fixedly mounted on the front side of the tank wall and is contacted with the inside of the tank wall, the spiral pipe is fixedly connected to the inner wall of the tank wall and is contacted with the temperature probe, a feed inlet is fixedly connected to the upper surface of the tank wall and is communicated with the inside of the tank wall, a round hole is formed in the upper surface of the tank wall, the electromagnetic recovery device is arranged inside the enzymolysis tank body and is located at the bottom of the enzymolysis tank body, the electromagnetic recovery device comprises a discharge port, a first electromagnetic recovery device, a second electromagnetic recovery device and a third electromagnetic recovery device, and the discharge port is arranged on the lower inner wall of the tank wall, electromagnetic recovery device one, electromagnetic recovery device two and three homodisperse of electromagnetic recovery device install at enzymolysis tank jar body tank bottoms, electromagnetic recovery device one, electromagnetic recovery device two and three surfaces of electromagnetic recovery device are the plane that has the radian a little, the lower extreme fixedly connected with drain hole of discharge gate, the drain hole communicates with each other with the inside of discharge gate, the inside of round hole is provided with agitating unit, round hole and agitating unit adaptation, agitating unit passes through the bearing with the round hole and links together.

Preferably, screw thread one has been seted up on the inner wall of feed inlet, the top of feed inlet is provided with the feed inlet lid, the lower fixed surface of feed inlet lid is connected with the stopper, stopper and feed inlet adaptation, screw thread two has been seted up in the outside of stopper, screw thread two and screw thread one adaptation, feed inlet lid and feed inlet link together through screw thread one and screw thread two.

Preferably, a first magnet cover, a second magnet cover and a third magnet cover are arranged above the discharge port, the first magnet cover, the second magnet cover and the third magnet cover are placed on the wall of the tank, the first magnet cover, the second magnet cover and the third magnet cover are attached to each other in pairs, the first magnet cover is fixedly connected with a first spring on the surface close to the first electromagnetic recovery device, the end, far away from the first magnet cover, of the first spring is fixedly connected to the first electromagnetic recovery device, the second magnet cover is fixedly connected with a second spring on the surface close to the second electromagnetic recovery device, the end, far away from the second magnet cover, of the second spring is fixedly connected to the second electromagnetic recovery device, the third magnet cover is fixedly connected to the surface close to the third electromagnetic recovery device, and the end, far away from the third magnet cover, of the third spring is fixedly connected to the third electromagnetic recovery device.

Preferably, agitating unit includes agitator motor, stirring post and stirring leaf one, agitator motor's output shaft fixedly connected with stirring post, the round hole is inserted to the stirring post lower extreme, the stirring post is in the inside of jar wall, the middle part of stirring post is provided with stirring leaf one, the lower part of stirring post is provided with stirring leaf two, stirring leaf one is the same with stirring leaf two.

Preferably, stirring leaf one includes column spinner one, column spinner two and acanthus leaf, the left side fixedly connected with column spinner one of column spinner, the right side fixedly connected with column spinner two of column spinner one and column spinner two's the outside is provided with the acanthus leaf, the opening has been seted up at the middle part of acanthus leaf, round groove one has been seted up on the left wall of opening, the round groove cup joints in the outside of column spinner one, round groove one and column spinner one adaptation, round groove two has been seted up on the right wall of opening, round groove cup joints in the outside of column spinner two, round groove two and column spinner two adaptations.

Preferably, the material of the tank body of the enzymolysis tank is hard plastic or glass fiber reinforced plastic.

Preferably, the heating or cooling mode of the tank body of the enzymolysis tank is spiral pipe heating or cooling.

Preferably, the coil tube material is a copper tube or an alloy material.

Preferably, the inner part of the tank of the stirring device is made of hard plastic or glass fiber reinforced plastic.

(III) advantageous effects

Compared with the prior art, the utility model provides an enzymolysis tank is retrieved to efficient immobilized enzyme electromagnetism possesses following beneficial effect:

1. the efficient immobilized enzyme electromagnetic recovery enzymolysis tank carries out effective recovery of magnetic immobilized enzyme materials through the electromagnetic recovery device, when the enzyme catalysis reaction is carried out, the electromagnetic recovery device positioned at the bottom of the tank is not electrified and does not have magnetism, the magnetic immobilized enzyme materials can be uniformly dispersed in the system to carry out catalytic reaction under the stirring action, when the reaction is finished, the electromagnetic recovery device is electrified, the magnetic immobilized enzyme materials are adsorbed on the electromagnetic recovery device I, the electromagnetic recovery device II and the electromagnetic recovery device III under the combined action of the sedimentation action and the magnetic adsorption action, enzyme catalysis products and residual substrates can be collected through the bottom discharge hole, after the collection is finished, new substrate feed liquid is added, the electromagnetic recovery device is powered off, and under the stirring action, the magnetic immobilized enzyme materials can be uniformly dispersed in the system again to carry out the enzyme catalysis reaction, the cost of enzymolysis is saved by effectively recovering the magnetic immobilized enzyme material.

2. The efficient immobilized enzyme electromagnetic recovery enzymolysis tank is characterized in that a first stirring blade and a second stirring blade are additionally arranged on the outer side of a stirring column, a blade plate of the first stirring blade is different from a common stirring device, the blade plate is not fixedly connected with the stirring column, but is movably connected with the first rotating column and the second rotating column, the blade plate is driven by a stirring motor to horizontally stir during stirring, and the blade plate can move up and down by taking the first rotating column and the second rotating column as an axis due to uneven front and back stress of the blade plate during re-stirring, so that the stirring can be performed in the vertical direction, particularly, the electromagnetic recovery device which just adsorbs a magnetic immobilized enzyme material is matched, and the first stirring blade and the second stirring blade are stirred up and down during next enzyme catalytic reaction after adsorbing the magnetic immobilized enzyme material, the magnetic immobilized enzyme material adsorbed on the electromagnetic recovery device can be rapidly and uniformly dispersed in the system in an accelerated manner, so that the enzymolysis process is accelerated, and the working speed is improved.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the internal structure of the tank wall of the present invention;

FIG. 3 is a schematic structural view of the electromagnetic recovery device of the present invention;

fig. 4 is a schematic structural view of the stirring device of the present invention.

In the figure: 1 enzymolysis tank body, 101 tank wall, 102 temperature probe, 103 helix tube, 104 feeding hole, 105 screw thread I, 106 feeding hole cover, 107 cover plug, 108 screw thread II, 109 round hole, 2 electromagnetic recovery device, 201 discharge hole, 202 electromagnetic recovery device I, 203 electromagnetic recovery device II, 204 electromagnetic recovery device III, 205 magnet cover I, 206 magnet cover II, 207 magnet cover III, 208 spring I, 209 spring II, 210 spring III, 211 discharge hole, 3 stirring device, 301 stirring motor, 302 stirring column, 303 stirring blade I, 304 stirring blade II, 305 rotating column I, 306 rotating column II, 307 blade plate, 308 through hole, 309 round groove I, 310 round groove II.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, an efficient immobilized enzyme electromagnetic recovery enzymolysis tank comprises an enzymolysis tank body 1, an electromagnetic recovery device 2 and a stirring device 3, wherein the enzymolysis tank body 1 is made of hard plastic or glass fiber reinforced plastic, the enzymolysis tank body 1 is heated or cooled by a spiral pipe 103 in a heating or cooling mode, the enzymolysis tank body 1 comprises a tank wall 101, a temperature probe 102 and a spiral pipe 103, the temperature probe 102 is fixedly installed on the front side of the tank wall 101, the temperature probe 102 is in contact with the inside of the tank wall 101, the spiral pipe 103 is fixedly connected to the inner wall of the tank wall 101, the spiral pipe 103 is made of copper pipe or alloy material, the spiral pipe 103 is in contact with the temperature probe 102, a feed inlet 104 is fixedly connected to the upper surface of the tank wall 101, the feed inlet 104 is communicated with the inside of the tank wall 101, a round hole 109 is formed in the upper surface of the tank wall 101, electromagnetic recovery device 2 is located the tank bottoms portion of the enzymolysis tank jar body 1, electromagnetic recovery device 2 includes discharge gate 201, electromagnetic recovery device 202, two 203 and three 204 of electromagnetic recovery device, discharge gate 201 is seted up on the lower inner wall of jar wall 101, electromagnetic recovery device 202, two 203 and three 204 of electromagnetic recovery device homodisperse are installed at enzymolysis tank jar 1 tank bottoms of body, electromagnetic recovery device 202, two 203 and three 204 of electromagnetic recovery device surfaces are the plane that has the radian a little, the lower extreme fixedly connected with drain hole 211 of discharge gate 201, drain hole 211 communicates with each other with the inside of discharge gate 201, the inside of round hole 109 is provided with

agitating unit

3, 3 jar insides of agitating unit divide into hard plastic or glass steel, round hole 109 and agitating unit 3 adaptation, agitating unit 3 links together through the bearing with round hole 109.

The inner wall of the feed inlet 104 is provided with a first thread 105, a feed inlet cover 106 is arranged above the feed inlet 104, the lower surface of the feed inlet cover 106 is fixedly connected with a cover plug 107, the cover plug 107 is matched with the feed inlet 104, the outer side of the cover plug 107 is provided with a second thread 108, the second thread 108 is matched with the first thread 105, and the feed inlet cover 106 and the feed inlet 104 are connected together through the first thread 105 and the second thread 108.

A first magnet cover 205, a second magnet cover 206 and a third magnet cover 207 are arranged above the discharge port 201, the first magnet cover 205, the second magnet cover 206 and the third magnet cover 207 are placed on the tank wall 101, the first magnet cover 205, the second magnet cover 206 and the third magnet cover 207 are attached in pairs, the first magnet cover 205 is fixedly connected with a first spring 208 on the side close to the first electromagnetic recovery device 202, the end, away from the first magnet cover 205, of the first spring 208 is fixedly connected to the first electromagnetic recovery device 202, the second magnet cover 206 is fixedly connected with a second spring 209 on the side close to the second electromagnetic recovery device 203, the end, away from the second magnet cover 206, of the second spring 209 is fixedly connected to the second electromagnetic recovery device 203, the side, close to the third electromagnetic recovery device 204, of the third magnet cover 207 is fixedly connected with a third spring 210, and the end, away from the third magnet cover 207, of the third spring 210 is fixedly connected to the third electromagnetic recovery device 204.

Agitating unit 3 includes agitator motor 301, stirring post 302 and stirring leaf 303, agitator motor 301's output shaft fixedly connected with stirring post 302, and round hole 109 is inserted to stirring post 302 lower extreme, and stirring post 302 is in the inside of jar wall 101, and the middle part of stirring post 302 is provided with stirring leaf 303, and the lower part of stirring post 302 is provided with stirring leaf two 304, and stirring leaf 303 is the same with stirring leaf two 304.

The first stirring blade 303 comprises a first rotating column 305, a second rotating column 306 and a blade 307, the first rotating column 305 is fixedly connected to the left side of the stirring column 302, the second rotating column 306 is fixedly connected to the right side of the stirring column 302, the blade 307 is arranged on the outer sides of the first rotating column 305 and the second rotating column 306, a through port 308 is formed in the middle of the blade 307, a first circular groove 309 is formed in the left wall of the through port 308, the first circular groove 309 is sleeved on the outer side of the first rotating column 305, the first circular groove 309 is matched with the first rotating column 305, a second circular groove 310 is formed in the right wall of the through port 308, the second circular groove 310 is sleeved on the outer side of the second rotating column 306, and the second circular groove 310 is matched with the second rotating column.

Example (b):

referring to fig. 1, adding enzymolysis substrate fish soluble slurry and protease immobilized by F3O4 magnetic immobilized material from a feed inlet 104 according to the proportion of 0.5% of enzyme dosage, diluting feed liquid by 5 times with tap water, opening a stirring device 3, stirring at the speed of 100r/min, introducing hot water from a spiral pipe 103, controlling the temperature of an enzymolysis system at 50 ℃ and keeping the temperature, after 5h of enzymolysis, electrifying an electromagnetic recovery device 2, opening a discharge port 211, discharging the enzymolysis high free amino acid fish soluble slurry from the discharge port 211 in the discharge process, adsorbing the magnetic immobilized protease on the electromagnetic recovery device 2, after finishing discharging, powering off the electromagnetic recovery device 2, closing the discharge port 211, adding new fish soluble slurry material from the feed inlet 104, opening the stirring device 3, releasing the magnetic immobilized protease in the system, and continuing enzyme catalysis reaction.

When in use, the utility model is used,

the first step is as follows: the effective recovery of the magnetic immobilized enzyme material is carried out by the electromagnetic recovery device 2, and when the enzyme catalytic reaction is carried out, the electromagnetic recovery device 2 positioned at the bottom of the tank is not electrified and has no magnetism, the magnetic immobilized enzyme material can be uniformly dispersed in the system for catalytic reaction under the stirring action, when the reaction is finished, the electromagnetic recovery device 2 is electrified, the magnetic immobilized enzyme material is adsorbed on the first electromagnetic recovery device 202, the second electromagnetic recovery device 203 and the third electromagnetic recovery device 204 under the combined action of the sedimentation effect and the magnetic adsorption effect, the enzyme catalysis product and the residual substrate, can be collected through the bottom discharge hole 211, after collection is finished, new substrate feed liquid is added, the electromagnetic recovery device 2 is powered off, under the stirring action, the magnetic immobilized enzyme material can be uniformly dispersed in the system again for enzyme catalysis reaction, so that the cost of enzymolysis is saved by effectively recovering the magnetic immobilized enzyme material.

The second step is that: through the high-efficiency immobilized enzyme electromagnetic recovery enzymolysis tank, the stirring blade I303 and the stirring blade II 304 are additionally arranged outside the stirring column 302, the blade plate 307 of the stirring blade I303 is different from a common stirring device, the blade plate 307 and the stirring column 302 are not fixedly connected together, but are movably connected through the rotating column I305 and the rotating column II 306, when stirring is carried out, the blade plate 307 is driven by the stirring motor 301 to carry out horizontal stirring, when stirring is carried out again, because the front and back stress of the blade plate 307 is uneven, the blade plate 307 can also move up and down by taking the rotating column I305 and the rotating column II 306 as axes, so that stirring can be carried out in the vertical direction, particularly, the stirring blade I303 and the stirring blade II 304 are stirred up and down when the magnetic immobilized enzyme material is adsorbed to carry out the next enzyme catalytic reaction, the magnetic immobilized enzyme material adsorbed on the electromagnetic recovery device 2 can be rapidly and uniformly dispersed in the system in an accelerated manner, so that the enzymolysis process is accelerated, and the working speed is improved.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides an enzymolysis tank is retrieved to efficient immobilized enzyme electromagnetism, includes the enzymolysis tank jar body (1), electromagnetism recovery unit (2) and agitating unit (3), the enzymolysis tank jar body (1) is including jar wall (101), temperature probe (102) and spiral pipe (103), its characterized in that: the utility model discloses an enzymolysis tank, including jar wall (101), front side fixed mounting of jar wall (101) has temperature probe (102), temperature probe (102) and jar wall (101) inside contact, fixedly connected with spiral pipe (103) on the inner wall of jar wall (101), spiral pipe (103) and temperature probe (102) contact, the last fixed surface of jar wall (101) is connected with feed inlet (104), feed inlet (104) and jar wall (101) inside communicate with each other, round hole (109) have been seted up to the upper surface of jar wall (101), the inside of the enzymolysis tank jar body (1) is provided with electromagnetism recovery unit (2), electromagnetism recovery unit (2) are located the tank bottoms portion of the enzymolysis tank body (1), electromagnetism recovery unit (2) include discharge gate (201), electromagnetism recovery unit one (202), electromagnetism recovery unit two (203) and electromagnetism recovery unit three (204), discharge gate (201) are seted up on the lower inner wall of jar wall (101), electromagnetism recovery unit one (202), electromagnetism recovery unit two (203) and electromagnetism recovery unit three (204) homodisperse are installed at enzymolysis tank jar body (1) tank bottoms, electromagnetism recovery unit one (202), electromagnetism recovery unit two (203) and electromagnetism recovery unit three (204) surfaces are the plane of radian slightly, the lower extreme fixedly connected with drain hole (211) of discharge gate (201), drain hole (211) communicates with each other with the inside of discharge gate (201), the inside of round hole (109) is provided with agitating unit (3), round hole (109) and agitating unit (3) adaptation, agitating unit (3) link together through the bearing with round hole (109).

2. The efficient electromagnetic recovery enzymolysis tank for the immobilized enzyme as claimed in claim 1, wherein: screw thread one (105) is seted up on the inner wall of feed inlet (104), the top of feed inlet (104) is provided with feed inlet lid (106), the lower fixed surface of feed inlet lid (106) is connected with lid stopper (107), lid stopper (107) and feed inlet (104) adaptation, screw thread two (108) are seted up in the outside of lid stopper (107), screw thread two (108) and screw thread one (105) adaptation, feed inlet lid (106) and feed inlet (104) link together through screw thread one (105) and screw thread two (108).

3. The efficient electromagnetic recovery enzymolysis tank for the immobilized enzyme as claimed in claim 1, wherein: a first magnet cover (205), a second magnet cover (206) and a third magnet cover (207) are arranged above the discharge port (201), the first magnet cover (205), the second magnet cover (206) and the third magnet cover (207) are placed on the tank wall (101), the first magnet cover (205), the second magnet cover (206) and the third magnet cover (207) are attached to each other in pairs, the first magnet cover (205) is fixedly connected with a first spring (208) on the surface close to the first electromagnetic recovery device (202), the end, away from the first magnet cover (205), of the first spring (208) is fixedly connected to the first electromagnetic recovery device (202), the second magnet cover (206) is fixedly connected with a second spring (209) on the surface close to the second electromagnetic recovery device (203), and the end, away from the second magnet cover (206), of the second spring (209) is fixedly connected to the second electromagnetic recovery device (203), and the third magnet cover (207) is fixedly connected with a third spring (210) on the surface close to the third electromagnetic recovery device (204), and the end, far away from the third magnet cover (207), of the third spring (210) is fixedly connected to the third electromagnetic recovery device (204).

4. The efficient electromagnetic recovery enzymolysis tank for the immobilized enzyme as claimed in claim 1, wherein: agitating unit (3) include agitator motor (301), stirring post (302) and stirring leaf (303), the output shaft fixedly connected with stirring post (302) of agitator motor (301), round hole (109) are inserted to stirring post (302) lower extreme, stirring post (302) are in the inside of jar wall (101), the middle part of stirring post (302) is provided with stirring leaf (303), the lower part of stirring post (302) is provided with stirring leaf two (304), stirring leaf one (303) and stirring leaf two (304) are the same.

5. The efficient electromagnetic recovery enzymolysis tank for the immobilized enzyme as claimed in claim 4, wherein: stirring leaf one (303) includes column spinner one (305), column spinner two (306) and lamina (307), the left side fixedly connected with column spinner one (305) of stirring post (302), the right side fixedly connected with column spinner two (306) of stirring post (302), the outside of column spinner one (305) and column spinner two (306) is provided with lamina (307), port (308) have been seted up at the middle part of lamina (307), round slot one (309) have been seted up on the left wall of port (308), round slot one (309) cup joint the outside at column spinner one (305), round slot one (309) and column spinner one (305) adaptation, round slot two (310) have been seted up on the right wall of port (308), round slot two (310) cup joint the outside at column spinner two (306), round slot two (310) and column spinner two (306) adaptation.

6. The efficient electromagnetic recovery enzymolysis tank for the immobilized enzyme as claimed in claim 1, wherein: the material of the enzymolysis tank body (1) is hard plastic or glass fiber reinforced plastic, the heating or cooling mode of the enzymolysis tank body (1) is spiral pipe (103) heating or cooling, the spiral pipe (103) material is copper pipe or alloy material, and the inner part of the stirring device (3) tank is hard plastic or glass fiber reinforced plastic.