CN211620507U

CN211620507U - Microbial detection suction filtration sample cup by thin film filtration method

Info

Publication number: CN211620507U
Application number: CN201922462191.4U
Authority: CN
Inventors: 徐永涛; 叶富友
Original assignee: Chongqing Pang Tong Medical Devices Co ltd
Current assignee: Chongqing Pang Tong Medical Devices Co ltd
Priority date: 2019-12-31
Filing date: 2019-12-31
Publication date: 2020-10-02
Anticipated expiration: 2029-12-31

Abstract

The utility model relates to a microbiological detection suction filtration sample cup by a membrane filtration method, which comprises a cup base, a cup cover, a cup body and an elastic sealing element, wherein the cup cover is arranged at the upper end part of the cup body; the cup base is provided with a supporting surface, the supporting surface is provided with a water outlet hole, and the cup base is provided with a connecting seat; the lower end part of the cup body is connected with the connecting seat; the connecting seat is provided with a sealing element mounting part, and the elastic sealing element is clamped between the sealing element mounting part and the lower end part of the cup body; a membrane pressing ring used for pressing the filtering membrane downwards is arranged in the cup body. The utility model discloses with the centre gripping of elastic sealing element between the lower tip of connecting seat and cup, guaranteed sealing performance, elastic sealing element does not contact with filtration membrane simultaneously, can not form liquid on filtration membrane and remain. The supporting surface is set to be a concave surface, which is beneficial to flattening the filtering membrane downwards during the suction filtration, so that the filtering membrane has no wrinkle after being filtered. Set up the filter pad, can avoid filtration membrane and holding surface direct contact on the one hand, on the other hand filter pad can play the effect that elastic support.

Description

Microbial detection suction filtration sample cup by thin film filtration method

Technical Field

The utility model relates to a microbiological detection field, concretely relates to film filtration method microbiological detection suction filtration sample cup.

Background

The purpose of the suction filtration of the liquid sample is to detect the microbial limit in the liquid sample by a more reliable 'membrane filtration method' specified by the regulation, such as detecting whether the microbial limit in the liquid sample of beverages, pharmacy, biological agents, biological reagents, hospital treatment water, disinfectant and the like exceeds the standard or not. The detection method specified in the national standard and the industrial standard (such as GB, GMP, pharmacopoeia) is a membrane filtration method, and one implementation mode of the membrane filtration method is to place a liquid sample in a filtration cup, arrange a filtration membrane for intercepting microorganisms in the filtration cup, discharge the liquid in the filtration cup by positive pressure or negative pressure, so that the microorganisms are intercepted on the filtration membrane, then flatly attach the filtration membrane to the surface of an agar culture medium for culture, count bacterial Colonies (CFU) growing on the filtration membrane, and judge whether the limit of the microorganisms exceeds the standard.

The suction filtration cup in the prior art generally comprises a cup base, a cup body with a lower end part detachably arranged on the cup base, and a cup cover detachably arranged on the upper end part of the cup body, wherein the cup base is provided with a water outlet, and the cup body is internally provided with a film pressing ring. When the suction filter cup is used, the filter membrane is supported on the cup seat, and the film pressing ring presses the filter membrane downwards to fix the filter membrane at the bottom of the suction filter cup. The existing suction and filtration cup has two sealing modes, one is to realize sealing by directly pressing the membrane filter by the squeeze film ring, and the other is to realize sealing by arranging a sealing ring between the squeeze film ring and the membrane filter.

The prior suction filter cup has the following problems:

1) if the squeeze film ring is used for directly pressing the filtering membrane to realize sealing, the squeeze film ring is made of hard materials, tight sealing is difficult to form, and the filtering membrane is easy to crush. The suction cup needs to be used for sucking samples in the suction cup in time under a clean environment (air pollution prevention) of a laboratory, otherwise, the water leakage phenomenon is easy to occur, great limitation and inconvenience are brought to sampling and experimental operation, and the suction cup is difficult to be used as a sampling transport container due to the water leakage phenomenon. The use of one sample cup is increased, which increases the cost, and the sample is loaded 2 times in at least one hundred thousand clean environments, which increases the risk of contamination.

2) If set up the sealing washer between press mold ring and filtration membrane and realize sealed, the sealing washer is pressed on filtration membrane, if in delivery port department plus stopper, then the suction filtration cup can regard as the sampling transport container to use. However, in the using process, it is found that residual liquid exists at the sealing ring and is difficult to be drained, and the residual liquid on the filtering membrane brings negative influence to the accuracy of the detection result, because when the filtering membrane is attached to the surface of the agar culture medium, the residual liquid is leaked and lost, and the residual liquid on the edge of the filtering membrane flows along the combined edge of the filtering membrane and the agar, so that the negative influence is directly brought to the accuracy of the detection result.

3) The supporting surface for supporting the filtering membrane is arranged on the cup seat, a plurality of grooves or holes communicated with the water outlet are formed in the supporting surface, the filtering membrane is directly supported on the supporting surface, after pressure application (positive pressure or negative pressure) filtration, the surface of the taken filtering membrane is uneven, the filtering membrane cannot be completely attached to the surface of a smooth agar culture medium, bacteria on the filtering membrane cannot grow completely, and negative effects are directly brought to the accuracy of detection results.

Disclosure of Invention

The utility model aims at providing a film filtration method microbiological detection suction filtration sample cup to alleviate or eliminate at least one foretell technical problem.

The utility model relates to a membrane filtration method microorganism detection suction filtration sample cup, which comprises a cup base, a cup cover, a cup body with openings at two ends and an annular elastic sealing element, wherein the cup cover is arranged at the upper end part of the cup body; the cup seat is provided with a supporting surface for supporting the filtering membrane, the supporting surface is provided with a water outlet hole, and the cup seat is provided with a connecting seat surrounding the periphery of the supporting surface; the lower end part of the cup body is sleeved on the periphery of the connecting seat, and the lower end part of the cup body is detachably connected with the connecting seat; the connecting seat is provided with a sealing element mounting part, the elastic sealing element is sleeved on the periphery of the connecting seat, and the elastic sealing element is clamped between the sealing element mounting part and the lower end part of the cup body; and a membrane pressing ring for pressing the filtering membrane downwards is arranged in the cup body.

Further, the supporting surface is a concave surface gradually concaved downwards from the edge of the supporting surface to the center of the supporting surface.

The filter device further comprises a filter pad for supporting the filter membrane, and the diameter of the filter pad is larger than the outer diameter of the part, used for compressing the filter membrane, on the membrane compression ring.

Furthermore, be equipped with on the connecting seat and be used for inlaying the dress inlay groove of filter pad has the advantage of being convenient for lay not deviating.

Further, the sealing member installation part is an annular flange protruding outwards from the outer side wall of the connecting seat, the elastic sealing member is supported on the annular flange, and the lower end part of the cup body downwards compresses the elastic sealing member.

Further, the lower tip of cup is equipped with inside convex first snap ring, be equipped with the outside convex second snap ring on the lateral wall of connecting seat, first snap ring clamps the downside of second snap ring, just first snap ring upwards supports tightly the second snap ring, in order to incite somebody to action the lower tip detachable of cup is connected on the connecting seat.

Furthermore, the cup cover is provided with air holes, and air filters are arranged in the air holes.

Furthermore, be equipped with the application of sample hole on the bowl cover, be equipped with the jam through the detachable mode in the application of sample hole.

Furthermore, a connecting cylinder extending downwards is arranged on the cup seat, the water outlet hole is communicated with the connecting cylinder, and the connecting cylinder is used for being connected with suction filtration equipment.

Furthermore, an induction cylinder extending downwards is arranged on the periphery of the cup base.

The utility model has the advantages that: through setting up connecting seat and sealing member installation department, with the elastic sealing member centre gripping between the lower tip of connecting seat and cup, guaranteed the sealing performance of membrane filtration method microorganism detection suction filtration sample cup, elastic sealing member does not contact with filtration membrane simultaneously, can not form liquid on filtration membrane and remain, has solved because filtration membrane after the suction filtration has the accuracy of remaining liquid and bringing negative effects's problem for the testing result. The supporting surface is set to be a concave surface, which is beneficial to flattening the filtering membrane downwards during the suction filtration, and the problem that the detection result is adversely affected due to the wrinkles on the filtering membrane is reduced or even eliminated. Set up the filter pad, on the one hand can avoid filtration membrane and holding surface direct contact, eliminate the possibility that produces the indentation on the filtration membrane, on the other hand filter pad can play the effect of elastic support for pressing tightly filtration membrane that the pressure membrane ring can stabilize, reduced the pressure membrane ring department and taken place the possibility of weeping, reduced the possibility that the filtration membrane was broken by pressure.

Drawings

FIG. 1 is a cross-sectional view of a vacuum filtration sample cup for microbiological detection by membrane filtration as described in the examples;

FIG. 2 is an enlarged view of the junction of the cup holder and cup body of FIG. 1;

FIG. 3 is an enlarged view of the connection between the lid and cup of FIG. 1;

fig. 4 is a schematic structural view of the cup holder.

In the figure: 1-cup body; 2, pressing a membrane ring; 3-a connecting part; 4, a cup cover; 5-cover edge; 6-air filter; 7, blocking; 8, a cup seat; 9-water outlet; 10-a support surface; 11-a connecting seat; 12-an annular flange; 13-connecting cylinder; 14-an induction cylinder; 15-a filter pad; 16-a filtration membrane; 17-an elastic sealing member; 18 — a first snap ring; 19-a second snap ring; 20-step surface; 21-a third snap ring, 22-a limit ring.

Detailed Description

The present invention will be further explained with reference to the accompanying drawings.

As shown in fig. 1 to 4, the microbiological detection suction filtration sample cup by using the membrane filtration method comprises a cup base 8, a cup cover 4, a cup body 1 with openings at two ends and an annular elastic sealing element 17, wherein the cup cover 4 is installed at the upper end part of the cup body 1, and the lower end part of the cup body 1 is provided with a connecting part 3 connected with the cup base 8; a supporting surface 10 for supporting the filtering membrane 16 is arranged on the cup seat 8, a water outlet hole 9 is arranged on the supporting surface 10, and a connecting seat 11 surrounding the periphery of the supporting surface 10 is arranged on the cup seat 8; the connecting part 3 is sleeved on the periphery of the connecting seat 11, and the connecting part 3 is detachably connected with the connecting seat 11; a sealing element mounting part is arranged on the connecting seat 11, an elastic sealing element 17 is sleeved on the periphery of the connecting seat 11, and the elastic sealing element 17 is clamped between the sealing element mounting part and the connecting part 3; a film pressing ring 2 for pressing the filtering film 16 downwards is arranged in the cup body 1. Through setting up connecting seat 11 and sealing member installation department, with elastic sealing member 17 centre gripping between connecting seat 11 and connecting portion 3, guaranteed the sealing performance of membrane filtration method microorganism detection suction filtration sample cup, elastic sealing member 17 can not contact with filtration membrane 16 simultaneously, can not form liquid on filtration membrane 16 and remain, has solved because filtration membrane 16 after the suction filtration has remaining liquid and brings negative effects's problem for the accuracy of testing result. In this embodiment, the cup holder 8, the cup lid 4 and the cup body 1 can be made of plastic. The microorganism detection suction filtration sample cup adopting the membrane filtration method has good sealing performance, can be used as a suction filtration cup, and can also be used as a sampling and transporting container after a sealing plug is plugged at the water outlet hole 9.

In the present embodiment, the support surface 10 is a concave surface gradually concaved downward from the edge of the support surface 10 toward the center of the support surface 10. The support surface 10 is concave, which is beneficial to flattening the filtering membrane 16 downwards during the suction filtration, and the problem that the detection result is adversely affected by the wrinkles appearing on the filtering membrane 16 is reduced or even eliminated. In order to improve the drainage effect, the supporting surface 10 is in a net shape, or as shown in fig. 4, the supporting surface 10 is formed by a plurality of ribs distributed in a divergent manner, and grooves communicated with the water outlet holes 9 are formed between the plurality of ribs.

In the embodiment, a filter pad 15 for supporting the filter membrane 16 is further included, and the diameter of the filter pad 15 is larger than the outer diameter of the part of the membrane pressing ring 2 for pressing the filter membrane 16. The filter pad 15 is capable of passing liquid through, and the filter pad 15 may be made of filter paper. Set up filter pad 15, on the one hand can avoid filtration membrane 16 and holding surface 10 direct contact, eliminate the possibility that produces the indentation on the filtration membrane 16, on the other hand filter pad 15 can play the effect of elastic support for pressing membrane ring 2 can stabilize compresses tightly filtration membrane 16, has reduced the possibility that the weeping takes place in membrane ring 2 department, has reduced the possibility that filtration membrane 16 was crushed.

In this embodiment, the connecting base 11 is provided with an insertion groove for inserting the filter pad 15. The upper end face of the connecting seat 11 is provided with a limiting ring 22 protruding upwards, and the limiting ring 22 is enclosed to form an embedded groove. The embedding groove is used for limiting the filter pad 15. When pressing membrane ring 2 compresses tightly filtration membrane 16, pressing membrane ring 2 is corresponding with the position of the up end of connecting seat 11, and filter pad 15 and filtration membrane 16 centre gripping are between pressing membrane ring 2 and the up end of connecting seat 11.

In this embodiment, the seal mounting portion is an annular flange 12 protruding outward from the outer side wall of the connecting socket 11, the elastic seal 17 is an annular packing, the elastic seal 17 is supported on the annular flange 12, and the lower end portion of the cup body 1 presses the elastic seal 17 downward to form a seal between the lower end portion of the cup body 1 and the annular flange 12. The elastic seal 17 may be made of silicone or rubber.

In other embodiments, the sealing member mounting portion may be an annular groove provided on the outer side wall of the connecting seat 11, and the elastic sealing member 17 is an O-ring fitted in the annular groove, and the O-ring is clamped between the inner side wall of the lower end portion of the cup body 1 and the outer side wall of the connecting seat 11 to form a seal.

In this embodiment, the lower end of the cup body 1 is provided with a first snap ring 18 protruding inwards, the outer side wall of the connecting seat 11 is provided with a second snap ring 19 protruding outwards, the first snap ring 18 is clamped at the lower side of the second snap ring 19, and the first snap ring 18 abuts against the second snap ring 19 upwards, so that the lower end of the cup body 1 is detachably connected to the connecting seat 11. For convenience of clamping, the lower side surface of the first clamping ring 18 is an inclined surface which gradually inclines upwards from outside to inside, the upper side surface of the first clamping ring 18 is an inclined surface which gradually inclines downwards from outside to inside, the lower side surface of the second clamping ring 19 is an inclined surface which gradually inclines upwards from inside to outside, and the upper side surface of the second clamping ring 19 is an inclined surface which gradually inclines downwards from inside to outside. Adopt above-mentioned scheme, elastic sealing element 17 can apply the ascending elastic force of lower tip for cup 1 to make first snap ring 18 upwards support second snap ring 19 tightly, guaranteed sealing performance promptly, make first snap ring 18 and second snap ring 19 joint firm again, also can form a sealing line between first snap ring 18 and the second snap ring 19 simultaneously. At the same time, the first clamping ring 18 is clamped to the lower side of the second clamping ring 19, so that the membrane pressing ring 2 can press the filtering membrane 16 downwards.

In this embodiment, the cup cover 4 is provided with an air vent, an air filter 6 is installed in the air vent, and a filter block for blocking microorganisms is arranged in the air filter 6. The filter block can be made of filter paper, and when in suction filtration, air can be supplemented into the membrane filtration method microorganism detection suction filtration sample cup through the air filter 6, and the air filter 6 has the function of preventing air pollution.

In this embodiment, the cup cover 4 is provided with a sample adding hole, and the sample adding hole is internally provided with a plug 7 in a detachable mode. The plug 7 is taken down, so that a sample can be added into the vacuum filtration sample cup for microbial detection by a membrane filtration method, and the operation is very convenient.

In this embodiment, a connecting cylinder 13 extending downwards is arranged on the cup base 8, the water outlet hole 9 is communicated with the connecting cylinder 13, and the connecting cylinder 13 is used for being connected with a suction filtration device. The suction filtration equipment can be a full-automatic membrane filtration suction filtration machine described in the Chinese patent application with the application number of CN 201720853552.6.

In this embodiment, the periphery of the cup holder 8 is provided with a downwardly extending induction cylinder 14. The induction cylinder 14 is used for triggering an induction switch on the suction filtration equipment to realize automatic suction filtration. The suction filtration equipment can be a full-automatic membrane filtration suction filtration machine described in the Chinese patent application with the application number of CN 201720853552.6.

As an implementation mode, the edge of the cup cover 4 is provided with a cover edge 5 extending downwards, the inner wall of the cover edge 5 is provided with a third inward-convex snap ring 21, the outer side wall of the upper end part of the cup body 1 is provided with a step surface 20 inclining upwards from inside to outside, the third snap ring 21 is clamped to the lower side of the step surface 20, the third snap ring 21 tightly pushes up the step surface 20 upwards, and the top wall of the cup cover 4 downwards presses an upper end opening of the cup body 1 so as to realize detachable connection between the cup cover 4 and the cup body 1. For the convenience of clamping, the lower side surface of the third clamping ring 21 is an inclined surface which gradually inclines upwards from outside to inside, and the upper side surface of the third clamping ring 21 is an inclined surface which gradually inclines downwards from outside to inside.

As an implementation mode, the upper end opening of the cup body 1 is large, and the lower end opening is small. The press mold ring 2 in the cup body 1 comprises a ring body with the diameter gradually reduced from top to bottom and a press mold part extending downwards from the lower end of the ring body, and the outer diameter of the press mold part is smaller than the diameter of the filter pad 15.

Claims

1. The utility model provides a film filtration method microorganism detects suction filtration sample cup which characterized in that: the cup comprises a cup base (8), a cup cover (4), a cup body (1) with openings at two ends and an annular elastic sealing element (17), wherein the cup cover (4) is arranged at the upper end part of the cup body (1); a supporting surface (10) for supporting the filtering membrane is arranged on the cup seat (8), a water outlet hole (9) is formed in the supporting surface (10), and a connecting seat (11) surrounding the periphery of the supporting surface (10) is arranged on the cup seat (8); the lower end part of the cup body (1) is sleeved on the periphery of the connecting seat (11), and the lower end part of the cup body (1) is detachably connected with the connecting seat (11); a sealing element mounting part is arranged on the connecting seat (11), the elastic sealing element (17) is sleeved on the periphery of the connecting seat (11), and the elastic sealing element (17) is clamped between the sealing element mounting part and the lower end part of the cup body (1); a film pressing ring (2) used for pressing the filtering film downwards is arranged in the cup body (1).

2. The membrane filtration-based microbiological detection pump-filtration sample cup according to claim 1, wherein: the supporting surface (10) is a concave surface which is gradually concave downwards from the edge of the supporting surface (10) to the center of the supporting surface (10).

3. The membrane filtration-based microbiological detection pump filtration sample cup according to claim 1 or 2, wherein: the filter device also comprises a filter pad (15) for supporting the filter membrane, wherein the diameter of the filter pad (15) is larger than the outer diameter of the part of the membrane pressing ring (2) for pressing the filter membrane.

4. The membrane filtration-based microbiological detection pump filtration sample cup according to claim 3, wherein: the connecting seat (11) is provided with an embedding groove for embedding the filter pad (15).

5. The membrane filtration-based microbiological detection pump-filtration sample cup according to claim 1, wherein: the sealing member installation part is outwards protruded from an annular flange (12) of the outer side wall of the connecting seat (11), the elastic sealing member (17) is supported on the annular flange (12), and the lower end part of the cup body (1) is downwards pressed on the elastic sealing member (17).

6. The membrane filtration-based microbiological detection pump-filtration sample cup according to claim 5, wherein: the cup is characterized in that a first inward-protruding clamping ring (18) is arranged at the lower end of the cup body (1), a second outward-protruding clamping ring (19) is arranged on the outer side wall of the connecting seat (11), the first clamping ring (18) is clamped at the lower side of the second clamping ring (19), and the first clamping ring (18) is abutted upwards against the second clamping ring (19) so as to detachably connect the lower end of the cup body (1) to the connecting seat (11).

7. The membrane filtration-based microbiological detection pump-filtration sample cup according to claim 1, wherein: the cup cover (4) is provided with air holes, and air filters (6) are installed in the air holes.

8. The membrane filtration-based microbiological detection pump-filtration sample cup according to claim 1, wherein: be equipped with the application of sample hole on bowl cover (4), be equipped with jam (7) through the detachable mode in the application of sample hole.

9. The membrane filtration-based microbiological detection pump-filtration sample cup according to claim 1, wherein: the cup is characterized in that a connecting cylinder (13) extending downwards is arranged on the cup base (8), the water outlet hole (9) is communicated with the connecting cylinder (13), and the connecting cylinder (13) is used for being connected with suction filtration equipment.

10. The membrane filtration-based microbiological detection pump-filtration sample cup according to claim 1, wherein: an induction cylinder (14) extending downwards is arranged on the periphery of the cup base (8).