CN211602632U

CN211602632U - Cell chip

Info

Publication number: CN211602632U
Application number: CN202020004299.9U
Authority: CN
Inventors: 郜恒骏; 朱诗樟; 亓垚; 陈宝光; 沈晓莹
Original assignee: Shanghai Outdo Biotech Co ltd
Current assignee: Shanghai Outdo Biotech Co ltd
Priority date: 2020-01-02
Filing date: 2020-01-02
Publication date: 2020-09-29
Anticipated expiration: 2030-01-02

Abstract

The utility model discloses a cell chip, include: a cover glass, a glass slide and a cell chip array block slice fixed on the glass slide; the cell chip array block section is a paraffin section with cell paraffin samples distributed in an array manner, and cell strains or primary cells subjected to dehydration treatment are embedded in the cell paraffin samples; each cell paraffin sample is fixed with a cell strain or primary cells; the section of the cell chip array block comprises two or more cell strains or primary cells with different sources. The utility model discloses a cell chip fixes multiple cell sample on same slide, can carry out unified experimental operation, and the result parallelism is good, and experimental error is little.

Description

Cell chip

Technical Field

The utility model relates to a device for immunocytochemistry experiment especially relates to a cell chip.

Background

Cells are the fundamental unit of morphology and function that make up an organism, and are themselves composed of many parts. The exploration of genes, protein functions and physiological and disease processes in life sciences and medicine has been largely based on the development of cellular levels. The cell chip is an important branch of biochip technology, and is characterized by that several identical or different cell specimens are arranged on same slide glass in the mode of regular array, and the in-situ histological research of same index can be implemented

Immunohistochemistry is to apply the basic principle of immunology, namely antigen-antibody reaction, namely the principle of specific combination of antigen and antibody, to determine the antigens (polypeptide and protein) in tissue cells by developing color of color developing agents (fluorescein, enzyme, metal ions and isotopes) for labeling the antibody through chemical reaction, and to perform positioning, qualitative and quantitative researches on the antigens, and the antigens are divided into two types according to the difference of experimental materials: 1) immunochemical techniques using tissue sections are known as immunohistochemical techniques; 2) immunochemical technology experiments using cell chips are known as immunocytochemical techniques. This is an important means for our study of the quantification and localization of protein expression in cells.

The traditional immunocytochemistry experiment is that a cell sample is fixed on a single glass slide for experimental operation in sequence, and the experimental method has the following defects:

1. low flux and low efficiency. Only one cytological specimen is usually fixed on a single slide, and the number of specimens that can be handled per experiment is very limited.

2. The experimental result has poor parallelism and large error. The cell samples on the slides are operated independently from each other, and the concentration of the antibody bound to each slide, the staining time, the elution time, and the like may be different, which may lead to the difference of the experimental results.

Therefore, in order to solve the above problems, a research tool with improved throughput, high efficiency and good parallelism of experimental results is needed to perform immunocytochemistry experiments.

Disclosure of Invention

The utility model aims to solve the technical problem that a cell chip is provided, it improves to have the flux, and detection efficiency is high, the good advantage of experimental result parallelism.

In order to solve the above technical problem, the utility model provides a cell chip, include: a cover glass, a glass slide and a cell chip array block slice fixed on the glass slide; the cell chip array block section is a paraffin section with cell paraffin samples distributed in an array manner, and cell strains or primary cells subjected to dehydration treatment are embedded in the cell paraffin samples; each cell paraffin sample is fixed with a cell strain or primary cells; the section of the cell chip array block comprises two or more cell strains or primary cells with different sources.

Specifically, a cell paraffin sample is located in a point on the section of the cell chip array block.

Specifically, the cell chip array block slice is obtained by slicing a cell chip array block, and the cell chip array block is obtained by filling cell paraffin blocks into holes distributed in an array form on a receptor paraffin block; the cell paraffin block is prepared by embedding dehydrated cell strains or primary cells in paraffin. After being sliced, in the original cell chip array block, the receptor wax block area corresponds to the receptor wax block slice, and the cell paraffin block area corresponds to the cell paraffin slice.

Specifically, the cell strain or primary cell is derived from human/animal.

Specifically, in the cell paraffin section, the proportion of cell strains or primary cells accounts for 40-80% of the whole section.

Specifically, the cell chip further comprises a carrier for recording the source information of all cell strains or primary cells.

Specifically, the cell chip further comprises a carrier on which quality inspection information of the cell chip is recorded.

Specifically, the carrier is in the form of an instruction book, an information card or a label and the like.

Specifically, the cell paraffin sections fixed with cell strains or primary cells of different sources are distributed at different positions of the array.

Specifically, the section of the cell chip array block contains 2, 3, 4, 5, 6, 7, 8, 9, 10 or more than 10 cell strains or primary cells with different sources; and at least one corresponding cell paraffin section is arranged in each cell strain or primary cell in the cell chip array block section.

The utility model discloses a cell chip contains the different cell strain of multiple source or primary cell simultaneously, and this multiple cell sample is fixed on same slide, can carry out unified experimental operation, and the result parallelism is good, and experimental error is little. For example, the concentration of the combined antibody, the dyeing time, the elution time and the like are very uniform, the reliability of the experimental result is high, and the experimental error is smaller than that of the traditional single sample.

The cell chip of the utility model has the characteristics of small volume and high information content. The detection of a plurality of cell samples can be completed in one experimental process, time, labor, cost and reagent are saved, limited sample resources can be utilized to the maximum extent, and the method has high efficiency.

The cell chip of the utility model can select various cell strains or primary cells from different sources according to the requirements, and can be customized according to the requirements of customers. A large number of cell samples can be arranged on one glass slide, the characteristics of high flux are realized, and the immunohistochemical experiment of a large number of samples can be simultaneously carried out.

Drawings

FIG. 1 is a schematic structural diagram of a cell chip according to an embodiment of the present invention.

FIG. 2 is a schematic structural diagram of a cell chip array block section in a cell chip according to the present invention.

Description of the symbols in the drawings:

1 is a glass slide;

2 is a cover glass;

3, slicing the cell chip array block;

31 is cell paraffin sample;

32 is receptor wax block slice;

33 are holes.

Detailed Description

The technical solutions of the present invention will be described clearly and completely below, and it should be apparent that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

Example one

A cell chip, as shown in fig. 1, comprising: a cover glass 2, a slide glass 1 and a section 3 of a biochip array block fixed on the slide glass 1. The section 3 of the cell chip array block is a paraffin section, a plurality of cell paraffin samples 31 are arranged on the paraffin section, and the cell paraffin samples 31 are distributed in an array manner. The cell paraffin sample 31 is embedded with dehydrated cell strains or primary cells. Each cell paraffin sample 31 has a cell line or primary cell fixed therein. The section 3 of the cell chip array block contains two or more cell lines or primary cells with different sources.

The cell chip contains two or more cell strains or primary cells with different sources, two or more cell samples are fixed on the same glass slide, unified experiment operation can be carried out, the result parallelism is good, and the experiment error is small. For example, the concentration of the combined antibody, the dyeing time, the elution time and the like are very uniform, the reliability of the experimental result is high, and the experimental error is smaller than that of the traditional single sample.

As shown in fig. 2, the section 3 of the cell chip array block is a receptor wax block section 32 as a substrate, an array of holes 33 are distributed on the surface of the receptor wax block section 32, and each hole 33 is used for filling a corresponding cell paraffin sample 31.

The section 3 of the cell chip array block is obtained by slicing the cell chip array block. The cell chip array block can be sliced continuously by a paraffin wheel slicer, and then the cell chip array block slice 3 is obtained after conventional operations such as slice spreading, slice dropping prevention treatment and the like. Wherein, the cell chip array block is obtained by filling cell paraffin blocks in holes distributed in an array on a receptor paraffin block. The cell paraffin block is prepared by embedding dehydrated cell strains or primary cells in paraffin. After slicing, the receptor wax block area in the original cell chip array block corresponds to the receptor wax block slice 32 in the cell chip array block slice 3, and the cell wax block area in the original cell chip array block corresponds to the cell wax slice 31 in the cell chip array block slice 3.

In this example, the cell line or primary cell is of human/animal origin. According to the customer requirements, the cell line or primary cell can be selected from various different sources. Cell samples from different sources are fixed at different locations.

In this embodiment, in the cell paraffin section, the proportion of the cell strain or the primary cell accounts for 40% -80% of the whole section, which is beneficial to improving better staining observation effect.

In the cell chip, cell paraffin sections 31 to which cell lines or primary cells of different origins are fixed are distributed at different positions of the array.

In the section 3 of the cell chip array block, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 10 or more cell lines or primary cells having different sources may be provided. Wherein, each cell strain or primary cell is provided with at least one corresponding cell paraffin section 31 in the cell chip array block section 3. In the case of the cell chip shown in FIG. 1, which is a 10X 10 array, 100 paraffin sections 31 can be theoretically provided. If each cell chip 31 has cell lines or primary cells with different sources, that is, the cell chip can accommodate up to 100 cell samples with different sources at the same time, the high-throughput characteristic is achieved.

The cell chip can be designed to be distributed in other arrays such as 4 × 4, 5 × 5, 8 × 8, etc. according to other requirements.

The cell chip also comprises a carrier for recording the source information of all cell strains or primary cells; the cell chip may further include a carrier on which quality control information of the cell chip is recorded. These carriers may be in the form of instructions, information cards or labels, etc.

Example two

This example describes a process for preparing the cell chip. The method comprises the following steps:

1) preparing a cell array block: respectively preparing more than 2 human/animal cell strains or primary cells with different sources into cell agarose gel, dehydrating, embedding paraffin, and respectively preparing into corresponding cell paraffin blocks. The cell paraffin block can be partially sliced firstly and then subjected to quality inspection by a hematoxylin-eosin staining method, and the cell paraffin block qualified in quality inspection can be used for subsequent operation. The array instrument punches holes in an array mode on the heated blank receptor wax block, and the cell wax block qualified in quality inspection is drilled by a coring hollow needle in sequence and is filled into the corresponding hole position of the receptor wax block to prepare the cell array block.

2) Cell array block sectioning: and (3) continuously slicing the cell array block obtained in the step 1) by using a paraffin wheel slicer, spreading the cell array block, pretreating the cell array block by using an anti-drop sheet, taking out the coded slide, and airing the cell array block at normal temperature.

3) Cell chip quality inspection: and (4) taking the slides with certain numbering intervals for quality inspection, and observing whether each core point is qualified under a microscope.

Generally, the quality inspection requirement includes at least 40-80% of the cell proportion of the whole section, so as to improve better dyeing observation effect. Other requirements for quality inspection include: the cell shape under the paraffin section has no obvious swelling or shrinkage, the cell size change is less than 10 percent, the cell density is moderate, and no overlapping extrusion exists.

In summary, the above embodiments are merely preferred embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalents, improvements, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims

1. A cell chip, comprising: a cover glass, a glass slide and a cell chip array block slice fixed on the glass slide;

the cell chip array block section is a paraffin section with cell paraffin samples distributed in an array manner, and cell strains or primary cells subjected to dehydration treatment are embedded in the cell paraffin samples; each cell paraffin sample is fixed with a cell strain or primary cells; the section of the cell chip array block comprises two or more cell strains or primary cells with different sources.

2. The cell chip of claim 1, wherein the cell chip array block section comprises a receptor wax block section, wherein the surface of the receptor wax block section is distributed with an array of holes, and the holes are filled with the cell paraffin sample.

3. The cell chip according to claim 1, wherein the section of the cell chip array block is obtained by sectioning a cell chip array block obtained by filling cell paraffin blocks into holes distributed in an array on a receptor paraffin block; the cell paraffin block is prepared by embedding dehydrated cell strains or primary cells in paraffin.

4. The cell chip of claim 1, wherein the cell line or primary cell is derived from a human/animal source.

5. The cell chip of claim 1, wherein the ratio of cell lines or primary cells in the paraffin section is 40-80% of the whole section.

6. The cell chip of claim 1, further comprising a carrier carrying information on the origin of all cell lines or primary cells.

7. The cell chip according to claim 1, wherein the cell chip further comprises a carrier carrying quality control information of the cell chip.

8. The cell chip of claim 6 or 7, wherein the carrier is an instruction book, an information card, or a label.

9. The cell chip of claim 1, wherein the paraffin sections of the cells immobilized with cell lines or primary cells of different origins are distributed at different positions of the array.

10. The cell chip of claim 1, wherein the section of the cell chip array block contains 2, 3, 4, 5, 6, 7, 8, 9, 10, or more than 10 cell lines or primary cells of different sources; and each cell strain or primary cell is provided with at least one corresponding cell paraffin sample point in the section of the cell chip array block.