CN211583661U - Operation room for taking eggs of live cattle - Google Patents

Abstract

The application relates to an operation room for egg taking of a live cow, which comprises: the egg picking chamber, the sterile egg picking chamber and the wall at the rear part of the egg picking chamber are externally provided with a cattle flock area. The outer wall of the front part of the egg collecting chamber is provided with a glass window convenient for observation and a pedestrian door for the entrance and exit of operators, the front part of the egg collecting chamber is provided with a cattle feeding area and a feeding groove for providing feed for the cattle, the middle part of the egg collecting chamber is provided with a retaining frame for fixing the cattle, and the rear part of the egg collecting chamber is an egg collecting operation area; a plurality of sets of the retaining frames are arranged side by side; the middle part of the fixing frame is provided with a supporting frame at the abdomen of the cow for lifting the cow when the cow is anesthetized; the rear part of the retaining frame is provided with a rear leg positioning rod for enabling two rear legs of the cow to tilt towards the front part of the body by moving the rear leg positioning rod when the cow is subjected to egg collection. The operation room can overcome certain defects of the existing operation room, particularly the problem of how to keep the body of a cow upright under the condition that the cow cannot stand because the cow is anesthetized during the live egg taking of the cow, and has the excellent effects described in the specification.

Description

Operation room for taking eggs of live cattle

Technical Field

The utility model belongs to the technical field of it is biological, a method that the live body adopted the ovum is related to, especially relate to an operation room that is used for ox live body to adopt the ovum.

Background

The embryo culture technology has important values for the breeding potential excavation, the rapid propagation and the germplasm resource protection of livestock. There are many factors that affect embryo in vitro culture and embryo in vitro preservation, among which oocyte in vitro maturation, in vitro fertilization, early embryo in vitro culture and embryo freezing are four major links in the in vitro embryo production process.

Bovine live body egg extraction (OPU) is a very practical embryo engineering technique for bovine embryo experimental manipulation and production and reproduction following In Vitro Fertilization (IVF) and Embryo Transfer (ET). This technique has been widely used for in vitro production of bovine embryos since the 20 th century, 80 s, reported the performance of bovine live body egg-taking operations under B-ultrasonic surveillance. The technology of taking the eggs from the live bodies of the cattle has the advantages of wide application, convenient operation, high efficiency, repeatability, small damage and the like. By combining the two technologies of live cow ovum taking and in vitro fertilization, the breeding potential of the improved cow can be fully improved, and high-quality embryos can be produced in large quantities.

The method for taking the bovine live body eggs provides a valuable method for producing high-quality bovine embryos in vitro. With the completion of the bovine genome sequencing and the drawing of new quantitative character sites, oocytes with clear genetic background are recovered by taking eggs from bovine living bodies, thereby providing valuable experimental materials for researching the bovine embryo development on the molecular level and finally generating remarkable economic and practical values for producing high-quality bovine embryos.

With the development of modern agricultural science and technology, in order to make full use of the breeding potential of elite cows and accelerate the genetic breeding process, it becomes necessary to apply a new efficient breeding technology in production practice. In-vivo egg taking (OPU) and In Vitro Fertilization (IVF) are new embryo engineering technologies that have been developed rapidly In the eighties of the twentieth century, and a large number of embryos with definite genetic pedigrees can be obtained by combining the OPU and the IVF, so that the generation interval is shortened. At present, the two technologies become important breeding technologies adopted by farmers in animal husbandry developed countries such as europe, the united states and oceania for expanding stock cow groups. However, with the conventional bovine embryo culture system (CR1aa and SOF liquid), the blastocyst development rate of bovine in vitro fertilization is low, and the embryo quality is far inferior to that of in vivo embryos, so that the pregnancy rate after embryo transfer recipient is low, and therefore how to improve the blastocyst development rate and the embryo quality becomes the focus of in vitro fertilization embryo production and research.

An important tool for taking the eggs of the cattle living bodies is a B-ultrasonic diagnostic instrument for animals, which is designed according to the B-ultrasonic principle and is commonly used in human clinic. The B-ultrasonic diagnosis has important significance for improving the management level of the animal husbandry and promoting the economic development of the animal husbandry. The method for evaluating the reproductive capacity of the buffalo by using the B ultrasonic diagnostic technology for animals can enable people to know and know the states of the ovary and the uterus of the buffalo in the oestrus cycle and pregnancy, better improve the reproductive performance and promote the genetic process of animals.

CN208876884U (application No. 201820459315.6, oxx) discloses a fixing frame for egg taking of living cow, which is believed to solve the problems that the fixing frame in the prior art can not be adjusted according to the body type of the cow and a cleaning device needs to be additionally arranged, and has the effects of firmly fixing the cow, having high stability, being combined with a cleaning device and being widely applicable to cows with different body types; the technical scheme is as follows: the frame body comprises a first fixed fence and a second fixed fence which are connected through a top cross brace with a groove; a water pipe connected with a shower nozzle is arranged inside the groove, and the shower nozzle is arranged above the first fixed fence; the inboard of first fixed rail is equipped with the slide rail, be fixed with the baoding rope on the slide rail, have a plurality ofly on the fixed rail of second and baoding rope complex bolt, the inboard of the fixed rail of second is equipped with the backing plate.

CN208625916U (application number 201820574924.6, Chuan nong) relates to an animal doctor and uses the apparatus, concretely relates to portable post fence formula ox is with retaining frame, including left rail, right rail, back rail, characterized by left rail, right rail and back rail enclose into retaining frame wholly, left rail and right rail parallel symmetry, the front end is the front prop, the rear end is the back prop, the upper end is the fence, the lower extreme is the lower fence, all be equipped with a well fence between upper fence and the lower fence, back rail upper end is the upper boom, the lower extreme is the lower boom, be equipped with a well boom between upper boom and the lower boom. The device is believed to have a simple structure, convenient movement, wide application and strong practicability.

CN204581585U (application number: 201520180375.0, animal husbandry) relates to a multifunctional detachable fixing frame for dairy cows, which is suitable for clinical diagnosis, treatment, operation, hoof repair and fixing of the dairy cows and veterinarians. Including base and post fence, its post fence comprises a plurality of vertical dead levers, horizontal dead lever and longitudinal fixing pole, the base comprises two parts at least, and is detachable fixed connection between the adjacent two parts, through fixed knot and fastener fixed connection between each dead lever of post fence, the base both sides correspond each vertical dead lever matching and are provided with the grafting sleeve pipe, be equipped with neck yoke, the hoof-repairing tool that is used for the head to fix on the post fence and preceding, back baoding chain and binder, pectoral girdle simultaneously. The utility model breaks through the inherent mode of the traditional fixing fence, veterinarians can adjust the size and the installation mode as required to fix and treat the milk cows, and the carrying and the transportation are convenient; and the floor space is small, the flexibility is high, the application range is wide, the use is convenient, the manufacturing cost is low, and the method is suitable for popularization and implementation.

However, because of the large volume of cattle, the existing in vivo egg taking operation table/room has such or other disadvantages, and therefore it is still highly desirable for those skilled in the art to provide a novel operation room for taking eggs from cattle in vivo to overcome some or some of the disadvantages of the existing operation room.

SUMMERY OF THE UTILITY MODEL

The invention aims to provide a new operating room for taking eggs from a living cow, which is expected to overcome certain defects of the existing operating room, particularly the problem of how to keep the body of the cow upright in the case that the cow cannot stand due to anesthesia during the live egg taking of the cow. It has been found that the design of the operating room for the egg taking of live bovine animals according to the present application perfectly solves the above problems.

To this end, the present application provides, in one aspect, an operating room for taking an egg from a living cow, (as shown in fig. 1 and 2, fig. 1 is a schematic top view of the operating room, and fig. 2 is a schematic relative position of a support frame and a hind leg positioning rod provided in a retaining frame on a head, an abdomen, and a hind leg of the cow), including:

the egg collecting chamber is provided with a glass window convenient for observation and a pedestrian door for the entrance and exit of operators on the outer wall of the front part, the front part in the egg collecting chamber is provided with a cattle feeding area and a feeding groove for providing feed for the cattle, the middle part in the egg collecting chamber is provided with a retaining frame for fixing the cattle, and the rear part in the egg collecting chamber is an egg collecting operation area;

the sterile egg picking chamber is arranged with a partition wall of the egg picking chamber, a glass window convenient for observation and a pedestrian door for operators to go in and out are arranged on the outer wall of the front part of the sterile egg picking chamber, the front part of the sterile egg picking chamber is isolated and arranged into a dressing room through a sliding door, the rear part of the sterile egg picking chamber is isolated and arranged into an egg picking room, a transfer window is arranged on the wall between the egg picking room and the egg picking chamber and used for transferring the cow eggs collected by the egg picking chamber to the egg picking room to pick the eggs, and a laboratory table for egg picking operation is arranged between the egg picking rooms;

a cattle flock area is arranged outside the wall at the rear part of the egg collecting chamber, the cattle flock area is separated from the egg collecting chamber through a lifting door, and a cattle flock area to be collected and a collected cattle flock area are arranged in the cattle flock area and are separated through a movable door;

it is characterized in that the preparation method is characterized in that,

1-5 sets of the retaining frames are arranged side by side (for example, 1-4 sets, for example, 2-3 sets, 2 sets are shown in figure 1, and egg taking operation of two cattle can be carried out simultaneously);

the middle part of the fixing frame is provided with a supporting frame at the abdomen of the cow for lifting the cow when the cow is anesthetized;

the rear part of the retaining frame is provided with a rear leg positioning rod for enabling the two rear legs of the cow to tilt towards the front part of the body by moving the rear leg positioning rod when the cow is subjected to egg collection (as indicated by arrows in figure 2).

According to the application, the operation room for taking the eggs of the living cattle is characterized in that a water pool is arranged at the corner of a pedestrian door inlet in the front of the egg taking chamber (so as to be convenient for cleaning).

According to this application operation room that the living body ovum of ox adopted the ovum usefulness, its characterized in that sets up at another wall corner department in the front portion of ovum room puts the thing platform.

According to this application operation room that the living body ovum of ox adopted the ovum usefulness, its characterized in that is in adopt the ovum equipment platform in the wall corner department of ovum room rear portion.

The operation room for taking the eggs of the living cattle is characterized in that an egg taking operation area at the rear part of the egg taking chamber is separated from the retaining frame through a lifting door.

According to the application, the operation room for taking the eggs of the living cattle is characterized in that the egg taking operation area is divided into different small areas at the rear part of the egg taking chamber through a movable door.

According to this application, operation room that the living body of ox adopted the ovum is used characterized in that, set up the neck clamp in the retaining frame front portion for with the neck clamp jail of ox when adopting the ovum.

The operation room for taking the eggs of the live cattle is characterized in that a water pool is arranged at the corner of a pedestrian door inlet in the front of the sterile egg picking chamber (so as to be convenient for cleaning).

The operation room for taking the eggs of the live cattle is characterized in that a coat and cap cabinet is arranged at the other wall corner in front of the sterile egg picking chamber.

According to this application, operation room that the living body of ox adopted ovum usefulness characterized in that picks up the ovum one end of laboratory bench and sets up the pond (so that clean).

The holding frame of the present application may adopt a design known in the art, however, a support frame for lifting the cow when the cow is anesthetized and a rear leg positioning rod for tilting the two rear legs of the cow toward the front of the body by moving the rear leg positioning rod when the cow is oviposited are further provided in the holding frame of the present application (as indicated by arrows in fig. 2). It has been surprisingly found that by arranging the hind leg positioning stick so that it is placed in advance at the rear of the hind leg and then moving the hind leg positioning stick forward of the cow's body so that the hind leg is tilted forward, the cow can more easily bring the ovum pick-up needle close to the follicle when handling this state in conjunction with in vivo ovum pick-up using the type-B ultrasonic diagnostic apparatus. On the other hand, since the cow is injected with an anesthetic when taking an egg, it is advantageous to provide a support frame for holding the cow up on its abdomen after the cow is unconscious.

The egg taking operation may be performed according to a method known in the art.

Any technical feature possessed by any one aspect of the present application or any embodiment of that one aspect may be equally applicable to any other embodiment or any embodiment of any other aspect, so long as they are not mutually contradictory, although appropriate modifications may be made to the respective features as necessary when applicable to each other. Various aspects and features of the disclosure are described further below.

All documents cited in this application are incorporated herein by reference in their entirety and to the extent that the meaning of such documents is inconsistent with this application, the express disclosure of this application controls. Further, the various terms and phrases used herein have the ordinary meaning as is known to those skilled in the art, and even so, it is intended that the present application be more fully described and interpreted herein, to the extent that such terms and phrases are not inconsistent with this known meaning and from the context in which such terms and phrases are expressed.

In the present application, the directional terms may be understood in conjunction with the description and the accompanying drawings, for example, when the "front" direction of a cow is expressed, the direction of the head of the cow is referred to as "front", and for example, when the "front" and "rear" of an egg taking chamber are described, the feeding area is relatively "front", and the egg taking operation area is "rear".

Drawings

FIG. 1 is a schematic plan view of an operation room for taking eggs from a living bovine body according to the present invention.

Fig. 2 is a schematic view of the relative positions of the support frame 18 and the rear leg positioning rod 24 provided in the retainer frame on the head, abdomen and rear leg of the cow.

Some reference numerals referred to in this application are summarized below: the egg picking room 1, the glass window 11, the pedestrian door 12, the cattle raising area 13, the water pool 14, the object placing table 15, the feed picking groove 16, the neck clamp 17, the support frame 18, the egg picking equipment table 19, the lifting door 20, the movable door 21, the cattle group area to be picked 22, the cattle group area to be picked 23, the rear leg positioning rod 24, the retaining frame 25, the egg picking operation area 26, the sterile egg picking room 3, the glass window 31, the pedestrian door 32, the dressing room 33, the water pool 34, the clothes and cap cabinet 35, the sliding door 36, the experiment table 37, the egg picking room 38, the water pool 39 and the transfer window 40.

Detailed Description

The present application can be further described by the following examples, however, the scope of the present application is not limited to the following examples. Those skilled in the art will appreciate that various changes and modifications can be made to the present application without departing from the spirit and scope of the application. The materials used in the tests and the test methods are generally and/or specifically described herein. Although many materials and methods of operation are known in the art for the purposes of this application, this application is nevertheless described herein in detail as far as possible. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are commercially available products.

The present application is further described with reference to the accompanying drawings. FIG. 1 is a schematic plan view of an operation room for taking eggs from a living bovine body according to the present invention. Fig. 2 is a schematic view of the relative positions of the support frame 18 and the rear leg positioning rod 24 provided in the retainer frame on the head, abdomen and rear leg of the cow.

An exemplary operating room for egg taking in a living bovine body, as shown in fig. 1 and 2, includes: the egg collecting chamber 1 is provided with a glass window 11 convenient for observation and a pedestrian door 12 for the entrance and exit of operators on the outer wall of the front part, the front part in the egg collecting chamber 1 is provided with a cattle feeding area 13 and a feeding groove 16 for providing feed for cattle, the middle part in the egg collecting chamber 1 is provided with a retaining frame 25 for fixing the cattle, and the rear part in the egg collecting chamber 1 is provided with an egg collecting operation area 26; the sterile egg picking chamber 3 is arranged with a partition wall of the egg picking chamber 1, a glass window 31 convenient for observation and a pedestrian door 32 for an operator to go in and out are arranged on the front outer wall of the sterile egg picking chamber 3, the front part of the sterile egg picking chamber 3 is isolated and arranged into a dressing room 33 through a sliding door 36, the rear part of the sterile egg picking chamber 3 is isolated and arranged into an egg picking room 38, a transfer window 40 is arranged on the wall between the egg picking room 38 and the egg picking chamber 1 and used for transferring the cow eggs collected by the egg picking chamber 1 to the egg picking room 38 for egg picking, and the egg picking room 38 is provided with a laboratory table 37 used for egg picking operation; a cattle flock area is arranged outside the wall at the rear part of the egg collecting chamber 1, the cattle flock area is separated from the egg collecting chamber 1 through a lifting door 20, a cattle flock area to be collected 22 and a collected cattle flock area 23 are arranged in the cattle flock area, and the two are separated through a movable door 21; wherein, the retaining frames 25 are provided with 1-5 sets (for example, 1-4 sets, for example, 2-3 sets, and 2 sets are shown in fig. 1), which can simultaneously carry out the egg-taking operation of two cattle, and can also be adjusted according to the cattle crowd scale, the number of operators and the size of the operation room); the middle part of the retaining frame 25 is provided with a supporting frame 18 at the abdomen of the cattle for lifting the cattle when the cattle is anesthetized; the rear part of the holding frame 25 is provided with a rear leg positioning rod 24 for tilting the two rear legs of the cow toward the front of the body by moving the rear leg positioning rod 24 when taking the cow's eggs (as indicated by arrows in fig. 2, it has been unexpectedly found that by arranging the rear leg positioning rod 24 in advance at the rear part of the rear legs and then moving the rear leg positioning rod 24 toward the front of the body of the cow, the rear legs are tilted forward, and the cow can more easily approach the ovum taking needle to the follicle when taking the ovum from the living body by using the B-ultrasonic diagnostic apparatus when handling this state, and it has been found that the best ovum taking operation effect can be achieved when the tilted rear legs are at an angle of 40 to 50 degrees with the ground).

In a preferred example of the operation room for taking eggs from live cattle of the present application, a water basin 14 is provided at the corner of the entrance of the pedestrian door 12 at the front of the egg taking chamber 1 (for cleaning).

In a preferred example of the operation room for taking the eggs of the living cattle, a placing platform 15 is arranged at the other wall corner of the front part of the egg taking chamber 1.

In a preferred example of the operation room for taking the eggs of the live cattle, an egg taking equipment platform 19 is arranged at the wall corner at the rear part of the egg taking chamber 1.

In a preferred example of the operation room for taking the eggs of the living cattle, an egg taking operation area 26 at the rear part of the egg taking chamber 1 is separated from the retaining frame 25 by the lifting door 20.

In a preferred example of the operation room for taking the eggs of the living cattle, the egg taking operation area 26 is divided into different small areas by a movable door 21 at the rear part of the egg taking chamber 1.

In a preferred example of the operation room for taking the eggs of the living cattle, a neck clamp 17 is arranged at the front part of the retaining frame 25 for clamping the neck of the cattle during egg taking.

In a preferred example of the operation room for taking the eggs of the live cattle, a water pool 34 is arranged at the corner of the entrance of the pedestrian door 32 at the front part of the sterile egg picking chamber 3 (for cleaning).

In a preferred example of the operation room for the live cow egg taking, a coat and cap cabinet 35 is arranged at the other corner of the front part of the sterile egg picking chamber 3.

In a preferred example of the operation room for taking the eggs of the living cow in the present application, a water pool 39 is provided at one end of the test table 37 of the egg picking room 38 (for cleaning).

The holding frame of the present application may adopt a design known in the art, however, a support frame 18 for lifting the cow when the cow is anesthetized and a rear leg positioning rod 24 for swinging the two rear legs of the cow forward of the body by moving the rear leg positioning rod 24 when the cow is oviposited are further provided in the holding frame of the present application (as indicated by arrows in fig. 2). It has been unexpectedly found that by arranging the hind leg positioning stick 24 so as to be placed in advance at the rear of the hind leg and then moving the hind leg positioning stick 24 forward of the cow's body so that the hind leg is tilted forward, the cow can more easily bring the ovum pick-up needle close to the follicle when handling this state in conjunction with the in vivo ovum pick-up by the type-B ultrasonic diagnostic apparatus. On the other hand, since the cow is injected with an anesthetic during egg collection, it is advantageous to provide a support frame 18 for holding the cow on its abdomen after the cow is unconscious.

In a preferred example of the operation room for taking the eggs of the living cattle, air conditioners can be further arranged in the egg taking chamber 1 and the sterile egg picking chamber 3 for use in a period with low air temperature. The bovine living body egg collection procedure room of the present application can be used as follows. Firstly, opening an air conditioner between egg taking rooms and an air conditioner between egg picking rooms, raising the indoor temperature to 20-25 ℃ (aiming at spring, autumn and winter), operating at room temperature in summer, making relevant preparation work before egg taking, for example, adding a delicious forage grass in an egg taking groove between egg taking rooms, connecting egg taking equipment, sterilizing an aseptic egg picking room by adopting ultraviolet irradiation for 10-15 minutes, and opening a water bath kettle, a microscope and a heating table of the egg picking room for preheating; driving the cows with eggs to be collected into a region to be collected, adjusting a movable door in an egg collection chamber, opening a heat-preservation lifting door, sequentially driving 2 cows with eggs to be collected into corresponding retaining frames, closing the lifting door, and adjusting the retaining frames to completely retain the cows; anaesthetizing the cattle, performing egg collection operation according to the flow, after the 1 st cattle is collected, sending the recovery liquid into an egg collection chamber through a transfer window, and then starting egg collection of the 2 nd cattle; marking the cattle number after the recovery liquid is taken in the egg picking chamber, starting washing and other egg picking operations, and putting the 1 st cattle into an incubator for pre-mature culture after the completion of the first cattle; after the 2 nd cow finishes egg collection, opening the lifting door, adjusting the movable door outside the door, discharging two cows to the collected area, then adjusting the movable door, driving in 2 cows to be collected again, and finishing all egg collection operations in sequence; and finally, after finishing, all the adopted cows are driven back to the original cowshed.

Present ox living body ovum picking work is mainly after fixing good ox through portable retaining frame or fixed retaining frame in the cowshed, carry out the ovum picking operation, send the recycle solution to the laboratory after the completion and pick up the ovum, there is certain transport distance generally, this application is in the same place living body ovum picking operation room and the design of picking up the ovum operation room, guarantee living body ovum picking work on the one hand and accomplish at indoor whole journey, avoid indoor outer temperature variation to the influence of oocyte quality, on the other hand reduces the outdoor transit time of recycle solution, guarantee that the oocyte is gathered out and can be picked up and put into the incubator as early as possible after external. By using the operation room for live cow egg taking, the average processing time of each cow can be reduced (the processing time of each cow can be reduced by 5-10 minutes), the availability of oocytes is improved (by 10% -20%), and the cleavage rate is improved (by 5% -10%).

Although the present application has been described in detail with respect to the general description and specific embodiments, it will be apparent to those skilled in the art that certain modifications or improvements may be made based on the present application. Accordingly, such modifications and improvements are intended to be within the scope of this invention as claimed.

Claims (10)

1. An operation room for taking eggs from a living cow comprises:

the egg collecting chamber is provided with a glass window convenient for observation and a pedestrian door for the entrance and exit of operators on the outer wall of the front part, the front part in the egg collecting chamber is provided with a cattle feeding area and a feeding groove for providing feed for the cattle, the middle part in the egg collecting chamber is provided with a retaining frame for fixing the cattle, and the rear part in the egg collecting chamber is an egg collecting operation area;

the sterile egg picking chamber is arranged with a partition wall of the egg picking chamber, a glass window convenient for observation and a pedestrian door for operators to go in and out are arranged on the outer wall of the front part of the sterile egg picking chamber, the front part of the sterile egg picking chamber is isolated and arranged into a dressing room through a sliding door, the rear part of the sterile egg picking chamber is isolated and arranged into an egg picking room, a transfer window is arranged on the wall between the egg picking room and the egg picking chamber and used for transferring the cow eggs collected by the egg picking chamber to the egg picking room to pick the eggs, and a laboratory table for egg picking operation is arranged between the egg picking rooms;

a cattle flock area is arranged outside the wall at the rear part of the egg collecting chamber, the cattle flock area is separated from the egg collecting chamber through a lifting door, and a cattle flock area to be collected and a collected cattle flock area are arranged in the cattle flock area and are separated through a movable door;

it is characterized in that the preparation method is characterized in that,

1-5 sets of retaining frames are arranged side by side;

the middle part of the fixing frame is provided with a supporting frame at the abdomen of the cow for lifting the cow when the cow is anesthetized;

the rear part of the retaining frame is provided with a rear leg positioning rod for enabling two rear legs of the cow to tilt towards the front part of the body by moving the rear leg positioning rod when the cow is subjected to egg collection.

2. The operation room for taking ova from a living bovine body according to claim 1, wherein a water pool is provided at a corner of a pedestrian door entrance at the front of the ova-taking chamber.

3. The operation room for taking ova from a living cow as claimed in claim 2, wherein a placement table is arranged at another wall corner at the front part of the ova-taking chamber.

4. The operation room for taking eggs from a living cow as claimed in claim 1, wherein an egg taking equipment platform is arranged at the wall corner at the rear part of the egg taking chamber.

5. The operation room for taking eggs from a living bovine body according to claim 1, wherein an egg taking operation area at the rear part of the egg taking chamber is separated from the holding frame by a lifting door.

6. The operation room for taking eggs from a living bovine body as claimed in claim 1, wherein the egg taking operation area is divided into different small areas by a movable door at the rear part of the egg taking chamber.

7. The operation room for collecting ovum of living cow as claimed in claim 1, wherein a neck clamp is provided at the front part of the retaining frame for clamping the neck of cow when collecting ovum.

8. The operation room for taking ova from a living cow as claimed in claim 1, wherein a water pool is provided at a corner of a pedestrian door entrance at the front part of the sterile ovum pick-up chamber.

9. The operation room for taking eggs from living cattle as claimed in claim 8, wherein a wardrobe is arranged at the other corner of the front part of the sterile egg picking chamber.

10. The operation room for taking ova from a living cow as claimed in claim 1, wherein a water pool is provided at one end of the test table of the ova-picking room.

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