CN211563340U - Centrifuging tube and centrifugal instrument that draw RNA of scientific research usefulness - Google Patents

Centrifuging tube and centrifugal instrument that draw RNA of scientific research usefulness Download PDF

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Publication number
CN211563340U
CN211563340U CN201922198366.5U CN201922198366U CN211563340U CN 211563340 U CN211563340 U CN 211563340U CN 201922198366 U CN201922198366 U CN 201922198366U CN 211563340 U CN211563340 U CN 211563340U
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tube
rna
centrifugal
stripe
scientific research
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CN201922198366.5U
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Chinese (zh)
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杜宏
张栋
郭小双
靳小雷
宗宪磊
宋国栋
都乐
赵竟依
杨常盛
赵久丽
马宁丹
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Plastic Surgery Hospital of CAMS and PUMC
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Plastic Surgery Hospital of CAMS and PUMC
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Abstract

The utility model relates to a scientific research is used draws centrifuging tube and centrifugal instrument of RNA relates to the centrifugation and draws the field. The centrifuge tube includes: centrifuging tube bottom is provided with the water conservancy diversion stripe of a plurality of interval arrangements, the water conservancy diversion stripe is followed the radial extension of centrifuging tube, the water conservancy diversion stripe including form in the arch of centrifuging tube bottom internal surface, be provided with the ring baffle of arranging along its week side on the centrifuging tube inner wall, ring baffle is located water conservancy diversion stripe top. The utility model is suitable for a scientific research room can solve RNA and draw by the degradation problem, reaches the effect that reduces the aspiration and improve RNA and draw the success rate.

Description

Centrifuging tube and centrifugal instrument that draw RNA of scientific research usefulness
Technical Field
The utility model relates to a RNA draws the field, especially relates to a centrifuging tube and centrifugal apparatus that draws RNA of scientific research usefulness.
Background
At present, in scientific research work, the centrifuging tube that the extraction RNA in-process used, the volume is less and the inner wall is smooth and level, and after the centrifugation, RNA attaches on the lateral wall of pipe, and alcohol is located the bottom of the tube. In the process of removing alcohol by using a pipette, RNA can be easily adsorbed away due to the limitation of space, and if the alcohol is waited for to naturally volatilize, the waiting time is long, and the RNA is easily degraded.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that to prior art not enough, provide a centrifuging tube and centrifugal apparatus that draws RNA of scientific research usefulness.
The utility model provides an above-mentioned technical problem's technical scheme as follows: the utility model provides a centrifuging tube that draws RNA that scientific research was used, the centrifuging tube bottom is provided with the water conservancy diversion stripe of a plurality of interval arrangements, the water conservancy diversion stripe is followed the radial extension of centrifuging tube, the water conservancy diversion stripe including form in the arch of centrifuging tube bottom internal surface, be provided with the ring baffle of arranging along its week side on the centrifuging tube inner wall, ring baffle is located water conservancy diversion stripe top.
The utility model has the advantages that: can disperse the different regions with the liquid in the centrifuge tube through the water conservancy diversion stripe of setting in the centrifuge tube inside, absorb the liquid in the centrifuge tube when the centrifugation of being convenient for is accomplished, the resistance of the protruding department of water conservancy diversion stripe can effectual increase when centrifugation is convenient for adhere to of RNA plaque simultaneously, can reduce the RNA through water conservancy diversion stripe and the ring baffle that sets up at the centrifuge tube bottom and draw the in-process, when removing liquid repeatedly, cause the aspiration and the degradation of RNA, improve the success rate that RNA drawed.
Further, still include: the annular baffle is a C-shaped baffle, and an opening of the C-shaped baffle is positioned above the interval between any two adjacent flow guide stripes.
The beneficial effect of adopting the further scheme is that: under the effect of centrifugal force, most RNA plaque is attached to the opening part below of C shape baffle, draws the more convenient and reduction mistake of RNA plaque and inhale the emergence of situation along the opening part.
Further, still include: still include the tube cap, the one end of tube cap with the top edge of centrifuging tube is connected, and the junction with the opening of C shape baffle corresponds from top to bottom.
The beneficial effect of adopting the further scheme is that: an RNA plaque gathering area is conveniently formed below the opening, and the extraction after the centrifugation is finished is convenient.
Further, still include: the flow guide stripe is provided with a groove for enabling liquid on two sides of the flow guide stripe to flow, the groove forms a circle, and the circle center of the circle is located on the axis of the centrifugal tube.
The beneficial effect of adopting the further scheme is that: the liquid between any two diversion stripes can flow conveniently, and the liquid in the centrifugal pipe can be sucked more conveniently.
Further, still include: the annular baffle is provided with a plurality of sieve holes for liquid volatilization, and the sieve holes are arranged above the interval between the two adjacent diversion stripes in a one-to-one correspondence manner.
The beneficial effect of adopting the further scheme is that: so that the liquid between any two adjacent guide stripes can be volatilized conveniently.
Further, still include: and a screen for guiding liquid is arranged in the sieve pores.
The beneficial effect of adopting the further scheme is that: facilitating the volatilization of the liquid.
Further, still include: the flow guide stripes are arranged at equal intervals, and the sieve holes are positioned above the middle of every two adjacent flow guide stripes.
The beneficial effect of adopting the further scheme is that: liquid such as alcohol among the diversion stripes can be conveniently led out from the sieve pores.
Further, still include: the annular baffle is located 3-4mm away from the bottom of the centrifuge tube.
The beneficial effect of adopting the further scheme is that: because the attachment area of the RNA plaque is 2-3mm away from the bottom of the centrifuge tube, the normal attachment of the RNA plaque is not influenced, and the function of facilitating the liquid discharge inside the centrifuge tube is realized.
Further, still include: the centrifuge tube is made of the following materials: polypropylene.
The beneficial effect of adopting the further scheme is that: light weight, stable to water, colorless, tasteless and nontoxic.
The utility model provides an another kind of technical scheme of above-mentioned technical problem as follows: the utility model provides a centrifugal instrument, contains any one of the scientific research usefulness draw centrifuging tube of RNA, the centrifuging tube is placed on the centrifugal instrument, just the centrifuging tube with the contained angle of the axis of centrifugal instrument is 45.
The beneficial effect of adopting the further scheme is that: can disperse the different regions with the liquid in the centrifuge tube through the water conservancy diversion stripe of setting at the inside centrifuge tube, because centrifuge tube slope is placed on centrifugal apparatus, absorb the liquid in the centrifuge tube when the centrifugation of being convenient for is accomplished more, resistance when the protruding department of water conservancy diversion stripe can effectually increase the centrifugation is convenient for adhere to of RNA plaque simultaneously, design through 45 contained angles can make the liquid in the centrifuge tube all in the opening part below of C shape baffle, make RNA plaque on a large scale the opening part below of C shape baffle because the design of slope at the centrifugal in-process, not only convenient simultaneously can also reduce the RNA and draw the in-process when drawing the RNA plaque like this, when removing liquid repeatedly, cause the mistake of RNA to inhale and degrade, improve the success rate that the RNA drawed.
Advantages of additional aspects of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
Drawings
Fig. 1 is a front view provided by an embodiment of the present invention;
fig. 2 is a top view provided by an embodiment of the present invention;
in the drawings, the components represented by the respective reference numerals are listed below:
1. c-shaped baffle plates, 2, flow guide stripes, 3, sieve holes, 301, a screen, 4, a plaque attaching area, 5, grooves, 6, a pipe cover, 7 and a connecting part.
Detailed Description
The principles and features of the present invention are described below in conjunction with the following drawings, the illustrated embodiments are provided to explain the present invention and not to limit the scope of the invention.
As shown in fig. 1, fig. 1 is a front view provided by an embodiment of the present invention, including: centrifuging tube bottom is provided with the water conservancy diversion stripe 2 of a plurality of interval arrangements, and water conservancy diversion stripe 2 is along the radial extension of centrifuging tube, and water conservancy diversion stripe 2 is provided with the annular baffle of arranging along its week side including forming the arch in centrifuging tube bottom internal surface on the centrifuging tube inner wall, and the annular baffle is located water conservancy diversion stripe 2 tops.
The beneficial effect who adopts above-mentioned scheme is: through setting up water conservancy diversion stripe 2 and the ring baffle at the centrifuging tube bottom, can reduce the RNA and draw the in-process, when removing liquid repeatedly, cause RNA's aspiration and degradation, improve RNA and draw the success rate.
It needs to be noted that water conservancy diversion stripe 2 just to the inside arch of centrifuging tube in centrifuging tube bottom toper position department, adopts 5 water conservancy diversion stripes 2 in this embodiment, and water conservancy diversion stripe 2 assembles in the centrifuging tube bottom, and water conservancy diversion stripe 2 and centrifuging tube integrated into one piece, the outer loop and the centrifuging tube inner wall connection of ring baffle.
Optionally, in some embodiments, the method may further include: the annular baffle is a C-shaped baffle 1, and an opening of the C-shaped baffle 1 is positioned above the interval between any two adjacent guide stripes 2.
The beneficial effect of adopting the further scheme is that: under the effect of centrifugal force, most RNA plaque is attached to the opening part below of C shape baffle 1, draws the more convenient and reduction mistake of RNA plaque and inhale the emergence of situation along the opening part.
It should be noted that the opening of the C-shaped baffle 1 needs to correspond to the joint 7, so that the RNA plaque can be attached to the position between the flow guide stripes 2 below the opening in the centrifugation process, and the size of the opening of the C-shaped baffle is about 2-3mm, which is convenient for extracting the RNA plaque and can effectively prevent the RNA plaque from being dissolved again by the alcohol lamp liquid to cause the centrifugation failure.
Optionally, in some embodiments, the method may further include: tube cap 6, the one end of tube cap 6 is connected with the top edge of centrifuging tube, and junction 7 corresponds from top to bottom with the opening of C-shaped baffle 1.
The beneficial effect of adopting the further scheme is that: an RNA plaque gathering area is conveniently formed below the opening, and the extraction after the centrifugation is finished is convenient.
It should be noted that, as shown in fig. 2, the size of the opening should not exceed the distance between two flow guiding stripes 2, and the BNA plaque will mostly attach to the lower part of the opening but also attach to a small part of the flow guiding stripes 2.
Optionally, in some embodiments, the method may further include: the flow guide stripe 2 is provided with grooves 5 for enabling liquid on two sides of the flow guide stripe 2 to flow, the grooves 5 form a circle, and the center of the circle is located on the axis of the centrifugal tube.
The beneficial effect of adopting the further scheme is that: the liquid between any two diversion stripes 2 can flow conveniently, and the liquid in the centrifugal pipe can be sucked more conveniently.
It should be noted that, as shown in fig. 2, the grooves 5 may not form a circle, and only the grooves 5 that can allow the liquid between any two flow guide stripes 2 to flow may be used, in this embodiment, each flow guide stripe 2 has one groove 5 and the direction is perpendicular to the flow guide stripe 2, in practical application, the number of the grooves 5 is not specifically required, and the direction of the grooves 5 is not specifically limited, as long as the two sides of the flow guide stripe 2 are communicated.
Optionally, in some embodiments, the method may further include: the annular baffle is provided with a plurality of sieve pores 3 for liquid volatilization, and the sieve pores 3 are arranged above the interval between the two adjacent guide stripes 2 in a one-to-one correspondence manner.
The beneficial effect of adopting the further scheme is that: so that the liquid between any two adjacent guide stripes 2 can be volatilized.
It should be noted that, as shown in fig. 2, the size of the sieve holes 3 is determined according to the size of the centrifuge tube, and the number of the sieve holes 3 is also determined according to the actual situation, in this embodiment, 4 sieve holes 3 are adopted, but the sieve holes 3 must be arranged above the interval between the diversion stripes 2, so as to facilitate the liquid in the centrifuge tube to be guided out and volatilized.
Optionally, in some embodiments, the method may further include: a screen mesh for guiding liquid is arranged in the sieve pore 3.
The beneficial effect of adopting the further scheme is that: facilitating the volatilization and the flow of the liquid.
It should be noted that, in the embodiment, the screen mesh on each screen hole 3 includes 8 small holes, and the actual operation can be properly adjusted according to the specification and size of the centrifuge tube
Optionally, in some embodiments, the method may further include: the diversion stripes 2 are arranged at equal intervals, and the sieve pores 3 are positioned above the middle of the two adjacent diversion stripes 2.
The beneficial effect of adopting the further scheme is that: so that the liquid such as alcohol and the like in the diversion strips 2 can be led out from the sieve pores 3.
It should be noted that in order to make the RNA plaque attached more regularly and to make the extraction more convenient, the positions of the sieve holes 3 are set to be in a state of two-by-two symmetry.
Optionally, in some embodiments, the method may further include: the annular baffle is located 3-4mm away from the bottom of the centrifuge tube.
The beneficial effect of adopting the further scheme is that: because the attachment area of the RNA plaque is 2-3mm away from the bottom of the centrifuge tube, the normal attachment of the RNA plaque is not influenced, and the function of facilitating the liquid discharge inside the centrifuge tube is realized.
It should be noted that the position of the ring-shaped baffle is not too high or too low, which is less than 3mm and is easy to affect the attachment of RNA plaque, and too high is not convenient for diversion and volatilization, so the ring-shaped baffle is best 3-4mm away from the bottom of the centrifuge tube.
Optionally, in some embodiments, the method may further include: the centrifuging tube is made of the following materials: polypropylene.
The beneficial effect of adopting the further scheme is that: light weight, stable to water, colorless, tasteless and nontoxic.
The utility model provides an another kind of technical scheme of above-mentioned technical problem as follows: the utility model provides a centrifugal instrument, contains any one of the above-mentioned scientific research usefulness draw centrifuging tube of RNA, the centrifuging tube is placed on centrifugal instrument, just the centrifuging tube with the contained angle of the axis of centrifugal instrument is 45.
The beneficial effect of adopting the further scheme is that: through setting up water conservancy diversion stripe 2 and the ring baffle at the centrifuging tube bottom, can reduce the RNA and draw the in-process, when removing liquid repeatedly, cause RNA's aspiration and degradation, improve RNA and draw the success rate.
It should be noted that, during centrifugation, the included angle between the centrifugal tube and the central axis of the centrifuge apparatus is 45 °.
It is understood that some or all of the alternative embodiments described above may be included in some embodiments.
It should be noted that the above embodiments are product embodiments corresponding to the previous method embodiments, and for the description of each optional implementation in the product embodiments, reference may be made to corresponding descriptions in the above method embodiments, and details are not described here again.
Adding into the centrifuge tube after taking out supernatant after chloroform centrifugation and isopropanol mixes, place the centrifuge tube on centrifuge instrument after alcohol purification, start centrifuge instrument, under the effect of centrifugal force, the molecule in the centrifuge tube begins the layering, because the slope is placed, most of RNA plaque is attached to 4 departments of plaque attached to the district, stop centrifugation, remove alcohol through the pipette gun from the vacancy position in the middle of C shape baffle 1, a small part of liquid that can't be removed through the pipette gun can volatilize out through sieve mesh 3 on C shape baffle 1, can take out the RNA plaque attached to the inner wall in the centrifuge tube when no liquid remains in the centrifuge tube.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily think of various equivalent modifications or replacements within the technical scope of the present invention, and these modifications or replacements should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (10)

1. The utility model provides a centrifuging tube of extraction RNA that scientific research was used, a serial communication port, the centrifuging tube bottom is provided with the water conservancy diversion stripe of a plurality of interval arrangements, the water conservancy diversion stripe is followed the radial extension of centrifuging tube, the water conservancy diversion stripe including form in the arch of centrifuging tube bottom internal surface, be provided with the ring baffle of arranging along its week side on the centrifuging tube inner wall, ring baffle is located water conservancy diversion stripe top.
2. The centrifugal tube for extracting RNA for scientific research according to claim 1, wherein the annular baffle is a C-shaped baffle, and the opening of the C-shaped baffle is located above the space between any two adjacent guide stripes.
3. The centrifugal tube for extracting RNA for scientific research according to claim 2, further comprising a tube cover, wherein one end of the tube cover is connected with the upper edge of the centrifugal tube, and the connection position of the tube cover is vertically corresponding to the opening of the C-shaped baffle.
4. The centrifugal tube for extracting RNA for scientific research according to any one of claims 1 to 3, wherein the guide stripe is provided with grooves for flowing liquid on two sides of the guide stripe, the grooves form a circle, and the center of the circle is located on the central axis of the centrifugal tube.
5. The centrifugal tube for extracting RNA for scientific research according to any one of claims 1 to 3, wherein a plurality of sieve holes for liquid volatilization are arranged on the annular baffle, and the sieve holes are arranged above the interval between two adjacent guide stripes in a one-to-one correspondence manner.
6. The centrifugal tube for scientific research extraction of RNA of claim 5, wherein a screen for guiding liquid is arranged in the screen hole.
7. The centrifugal tube for extracting RNA of claim 5, wherein the diversion stripes are arranged at equal intervals, and the screen holes are positioned above the middle of two adjacent diversion stripes.
8. The scientific RNA extraction centrifuge tube of any one of claims 1 to 3, wherein the ring baffle is located 3-4mm from the bottom of the centrifuge tube.
9. The centrifugal tube for extracting RNA according to claim 1, which is made of: polypropylene.
10. A centrifugal apparatus comprising the scientific research RNA extraction centrifugal tube of any one of claims 1 to 9, wherein the centrifugal tube is placed on the centrifugal apparatus, and an included angle between the centrifugal tube and a central axis of the centrifugal apparatus is 45 °.
CN201922198366.5U 2019-12-10 2019-12-10 Centrifuging tube and centrifugal instrument that draw RNA of scientific research usefulness Active CN211563340U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201922198366.5U CN211563340U (en) 2019-12-10 2019-12-10 Centrifuging tube and centrifugal instrument that draw RNA of scientific research usefulness

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201922198366.5U CN211563340U (en) 2019-12-10 2019-12-10 Centrifuging tube and centrifugal instrument that draw RNA of scientific research usefulness

Publications (1)

Publication Number Publication Date
CN211563340U true CN211563340U (en) 2020-09-25

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201922198366.5U Active CN211563340U (en) 2019-12-10 2019-12-10 Centrifuging tube and centrifugal instrument that draw RNA of scientific research usefulness

Country Status (1)

Country Link
CN (1) CN211563340U (en)

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