CN211562995U - Digital microfluidic system - Google Patents
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- CN211562995U CN211562995U CN201921669546.0U CN201921669546U CN211562995U CN 211562995 U CN211562995 U CN 211562995U CN 201921669546 U CN201921669546 U CN 201921669546U CN 211562995 U CN211562995 U CN 211562995U
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Abstract
The application is suitable for the technical field of micro-fluidic and provides a digital micro-fluidic system. The digital microfluidic system comprises a microfluidic chip and a control circuit, wherein the microfluidic chip comprises a bottom electrode plate, and the bottom electrode plate comprises a plurality of electrodes. The plurality of electrodes comprise control electrodes arranged in an array, the control circuit comprises a plurality of switches, the switches are in one-to-one correspondence with the control electrodes, and each switch comprises a first connecting position connected to the ground and a second connecting position connected with a power supply.
Description
Technical Field
The application belongs to the technical field of micro-fluidic, especially, relate to a digital micro-fluidic system.
Background
Two configurations of digital microfluidic systems are described in patent EP3210010B 1.
FIG. 1 shows a cross-sectional structure of a digital microfluidic system including a cap therein. The digital microfluidic system comprising a top cap comprises a top cap 25, a bottom electrode plate 15 and a control circuit. The top cover 25 comprises a substrate 24, a conductive layer 22 and a hydrophobic layer 20 which are connected in sequence, the bottom electrode plate 15 comprises a hydrophobic layer 18, a dielectric layer 16 and a substrate 12 which are connected in sequence, and an electrode array 14 (comprising a plurality of electrodes) which is embedded between the dielectric layer 16 and the substrate 12. A droplet moving space 30 is formed between the water-repellent layer 20 and the water-repellent layer 18. The control circuit includes a plurality of switches 28 and a power supply 26, one switch connected between one electrode and the high potential terminal of the power supply 26, the ground terminal of the power supply 26 and the conductive layer 22 both being grounded. In this manner, when any of the switches is in an on state, an electrode connected to the switch (referred to as an "on electrode") is at a high potential due to the connection with the power source 26, while the conductive layer 22 of the top cover 25 is at a low potential due to the ground, so that a certain potential difference is formed between the on electrode and the conductive layer 22. The potential difference, which may change the hydrophobicity of the surface of the dielectric layer at the position of the opening electrode and thus the contact angle of the surface of the dielectric layer with the droplet at the position of the opening electrode, may form a tangential force (as indicated by the arrow) that urges the droplet 42 to move when the potential difference is sufficiently large.
Fig. 2 shows a cross-sectional structure of a digital microfluidic system without a top cover. The digital microfluidic system without the top cover comprises a bottom electrode plate and a control circuit. The bottom electrode plate comprises a hydrophobic layer 18, a dielectric layer 16 and a substrate 12 which are connected in sequence, and an electrode array which is embedded between the dielectric layer 16 and the substrate 12. The electrode array includes a plurality of electrodes 14 arranged in an array with smaller ground electrodes 52 disposed between the electrodes 14. The control circuit includes a plurality of switches 28 and a power supply. A switch is connected between one of the electrodes 14 and the high potential terminal of the power supply, and the ground terminal of the power supply 26 is grounded. As such, when any one of the switches is in an on state, the electrode 14 (referred to as "on electrode") connected to the switch is at a high potential due to the connection with the power source, and the ground electrode 52 is at a low potential due to the ground, so that a certain potential difference is formed between the on electrode and the ground electrode 52. The potential difference, which may change the hydrophobicity of the surface of the dielectric layer at the position of the opening electrode and thus the contact angle of the surface of the dielectric layer with the droplet at the position of the opening electrode, may form a tangential force that urges the droplet 42 to move when the potential difference is sufficiently large.
In the digital microfluidic system with the top cover, the conducting layer of the top cover needs to be grounded, and in the digital microfluidic system without the top cover, a small grounding electrode needs to be arranged between electrodes connected with the switch, so that the packaging process of the digital microfluidic system is complicated.
Therefore, there is a need to provide a digital microfluidic system that is easy to package.
SUMMERY OF THE UTILITY MODEL
In view of this, the embodiments of the present application provide a microfluidic chip with a top cover and a microfluidic chip without a top cover, so as to solve the problem in the prior art that a conductive layer of a top cover needs to be grounded in the microfluidic chip with a top cover, and a smaller ground electrode needs to be arranged between electrodes connected to a switch in the microfluidic chip without a top cover, so that a packaging process of the microfluidic chip is complicated.
A first aspect of the embodiments of the present application provides a digital microfluidic system, digital microfluidic system includes microfluidic chip and control circuit, microfluidic chip includes bottom electrode board, bottom electrode board contains a plurality of electrodes, a plurality of electrodes are including being the control electrode that the array was arranged, control circuit includes a plurality of switches, switch and control electrode one-to-one, each the switch includes grounded first hookup location and the second hookup location of being connected with the power.
In some embodiments, the switch includes a reset selector and/or an optocoupler.
In some embodiments, the power supply comprises a signal generator, a voltage/level conversion device, or a power conversion device.
In some embodiments, the plurality of electrodes further comprises a ground electrode, the ground electrode being always grounded.
In some embodiments, the ground electrode surrounds the exterior of the control electrodes arranged in an array.
In some embodiments, the microfluidic chip is a capless microfluidic chip.
In some embodiments, the microfluidic chip further comprises a top cover, wherein a droplet moving space is formed between the top cover and the bottom electrode plate, and the top cover comprises a top cover conductive layer which is in a voltage suspension state.
In some embodiments, the top cover conductive layer has a size not smaller than a size of the electrodes laid on the bottom electrode plate.
In some embodiments, the power supply may provide a voltage signal or a current signal.
The embodiment of the application has the following possible beneficial effects:
the embodiment of the application provides a digital microfluidic system. The digital microfluidic system comprises a microfluidic chip and a control circuit, wherein the microfluidic chip comprises a bottom electrode plate, the bottom electrode plate comprises a plurality of electrodes, the control circuit respectively controls each electrode to be grounded or connected with a power supply, and the total area of the electrodes for controlling grounding is at least 5 times that of the electrodes connected with the power supply. Therefore, the potential difference capable of driving the liquid drops is formed, the switch control electrode is grounded, the top cover does not need to be grounded, or a smaller grounding electrode is arranged in the bottom electrode array, and the packaging difficulty of the digital microfluidic system is further reduced.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise.
FIG. 1 is a schematic cross-sectional view of a prior art digital microfluidic system including a top cap;
FIG. 2 is a schematic cross-sectional view of a prior art digital microfluidic system without a top cap;
fig. 3 is a schematic diagram illustrating relative positions of a ground electrode and a plurality of control electrodes arranged in an array in a digital microfluidic system according to an embodiment of the present disclosure;
FIG. 4 is a schematic cross-sectional view of a digital microfluidic system including a top cap according to an embodiment of the present disclosure;
FIG. 5 is a schematic diagram of the operation of the digital microfluidic system including a top cap of FIG. 4;
FIG. 6 shows an equivalent circuit model built based on a digital microfluidic system including a cap with a droplet positioned between an initial position electrode and a target position electrode;
FIG. 7(a) shows the case when the conductive layer of the top cover is grounded and a voltage V is applied to the target position electrodeextAn equivalent circuit diagram of time;
FIG. 7(b) when the top conductive layer is floating and voltage V is applied to the target site electrodeextAn equivalent circuit of time;
FIG. 8(a) shows a digital microfluidic system comprising a top cap provided by an embodiment of the present application;
FIG. 8(b) is a graph of a voltage simulation of a prior art digital microfluidic system including a cap on a voltage observation line;
fig. 9 is a schematic cross-sectional structure diagram of a cap-free digital microfluidic system provided in an embodiment of the present application;
fig. 10 is a schematic diagram of the operation of the cap-less digital microfluidic system of fig. 9.
Detailed Description
In order to make the technical solutions better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are all embodiments of the present application, not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
The terms "comprises" and "comprising," and any variations thereof, in the description and claims of this application and the drawings described above, are intended to cover non-exclusive inclusions. For example, a process, method, or system, article, or apparatus that comprises a list of steps or elements is not limited to only those steps or elements listed, but may alternatively include other steps or elements not listed, or inherent to such process, method, article, or apparatus. Furthermore, the terms "first," "second," and "third," etc. are used to distinguish between different objects and are not used to describe a particular order.
The embodiment of the application provides a digital microfluidic system which comprises a microfluidic chip and a control circuit. The microfluidic chip includes a bottom electrode plate comprising a plurality of electrodes. The control circuit is for controlling each electrode to be grounded or powered on, respectively, and the total area of the electrodes for controlling to be grounded is at least 5 times the total area of the electrodes for powering on. Therefore, the potential difference capable of driving the liquid drops is formed, the switch control electrode is grounded, the top cover does not need to be grounded, or a smaller grounding electrode is arranged in the bottom electrode array, and the packaging difficulty of the digital microfluidic system is further reduced.
In some embodiments, the plurality of electrodes may include control electrodes arranged in an array, the control circuit includes a plurality of switches, the switches correspond to the control electrodes one to one, and each switch includes a first connection position connected to the ground and a second connection position connected to the power supply. Optionally, the switch may include a reset selector, an optocoupler, or the like, or any combination thereof. In some embodiments, the area of each of the control electrodes may be equal. In still other embodiments, the areas of the control electrodes may not be equal.
In some embodiments, to increase the total area of the ground electrode, the plurality of electrodes may further include a ground electrode that is always grounded. Alternatively, as shown in fig. 4, the ground electrode may surround the outer portion of the control electrodes arranged in an array.
In some embodiments, the power supply may include a signal generator, a voltage/level translation device, or a power translation device. Alternatively, the power supply may provide a voltage signal or a current signal.
The following respectively describes two specific structures of the digital microfluidic system provided in the embodiments of the present application.
Referring to fig. 4, fig. 4 is a schematic cross-sectional structure view of a digital microfluidic system including a top cover according to an embodiment of the present disclosure. As shown in fig. 4, the digital microfluidic system including the top cover includes a microfluidic chip including the top cover and a control circuit. The microfluidic chip with the top cover comprises the top cover 25 and a bottom electrode plate, wherein the top cover 25 comprises a substrate 24, a conducting layer 22 and a hydrophobic layer 20 which are sequentially connected, and the bottom electrode plate comprises the hydrophobic layer 18, a dielectric layer 16, a substrate 12 which are sequentially connected, and an electrode array 34 which is embedded between the dielectric layer 16 and the substrate 12. A droplet moving space is formed between the water-repellent layer 20 and the water-repellent layer 18. The control circuit includes a power supply 26 and a plurality of switches 38, and electrode array 34 includes a plurality of control electrodes.
The switches are connected with the control electrodes in a one-to-one correspondence mode. The switch has at least two connection positions, when the switch is in a first connection position, the control electrode connected with the switch is connected with the power supply 26, and when the switch is in a second connection position, the control electrode connected with the switch is grounded. The conducting layer of the top cover is not grounded and is in a voltage suspension state.
Referring to fig. 5, fig. 5 is a schematic diagram illustrating the operation of the microfluidic chip including the top cap shown in fig. 4. As shown in fig. 5, by setting the connection position of each switch, one control electrode (referred to as "on electrode") is turned on with the power source 26 to be at a high potential, and the other control electrode (referred to as "off electrode") is grounded to be at a low potential.
In some embodiments, when the number of unopened electrodes is sufficiently large, a sufficiently large capacitance is formed between all unopened electrodes at the bottom and the conductive layer 22 at the top, so that the voltage between all unopened electrodes at the bottom and the conductive layer 22 at the top is approximately zero. In other words, the potential of the top conductive layer 22 is close to the ground potential (low potential) of the unopened electrode. Thus, a potential difference is created between the on electrode at a high potential and the conductive layer 22 near ground potential, which when large enough can cause the droplets 42 to move. In some embodiments, when the ratio of the total area of the unopened electrodes to the total area of the opened electrodes is not less than 5, the voltage of the conductive layer of the top cover can be lower than 20% of the standard voltage of the power supply, thereby effectively controlling the droplet movement. Further, the control electrodes in the electrode array may be designed to be of equal area, so that optionally, in order to form a sufficiently large capacitance as much as possible by the control, the size of the conductive layer 22 of the top cover 25 may also be configured to be not smaller than the size of the electrodes laid on the bottom electrode plate, for example, the top cover 25 may completely cover the electrode array 34.
Referring to fig. 6 and 7 in combination, fig. 6 shows an equivalent circuit model established based on a digital microfluidic system including a cap in a case where a droplet is located between an initial position electrode 1 and a target position electrode 2, and fig. 7(a) shows a case where a conductive layer of the cap is grounded and a voltage V is applied to the target position electrode 2extFIG. 7(b) is an equivalent circuit diagram when the top cover conductive layer is floated and a voltage V is applied to the target position electrode 2extThe equivalent circuit of time. Wherein the area of each electrode is equal. Capacitor 3 (C)d1) The equivalent capacitance of the droplet portion above the initial position electrode 1, capacitance 4 (C)d2) Is the equivalent capacitance of the portion of the droplet above the target site electrode 2. Capacitor 5 (C)dl) The capacitance 6 (C) is the equivalent capacitance of the dielectric layer above the initial position electrode 1dl) The equivalent capacitance of the dielectric layer above the target location electrode 2 is equal to each other (since the capacitance 5 is identical to the capacitance 6, the same reference numerals are used). The capacitor 7 is the initial position electrode 1 (C)m1) Equivalent capacitance of the part of the medium above which the drop is wrapped, capacitance 8 (C)m2) Is the equivalent capacitance of the portion of the medium surrounding the droplet above the target site electrode 2. When the conductive layer of the top cover is grounded and a voltage V is applied to the target position electrode 2extThe equivalent circuit is shown in FIG. 7a, the voltage (V) across the capacitor 6eff) It is determined whether the droplet can be successfully driven. When the top cover conducting layer is suspended and voltage V is applied to the target position electrode 2extEquivalent circuit the voltage (V) across the capacitor 6 is shown in FIG. 7beff') determines whether a droplet can be successfully driven.
Wherein, VeffThe formula is as follows:
Veffthe formula of calculation of' is as follows:
when the number of unopened electrodes is k times the number of opened electrodes, VeffThe calculation formula of' becomes:
therefore Veff' ≈ Veff when κ is large enough, i.e. the top cap voltage suspension is approximately the same as the digital microfluidic system effect with the top cap grounded.
Referring to fig. 5 and 8 in combination, fig. 8(a) shows a voltage simulation graph of a microfluidic chip with a top cover (voltage floating, i.e. not grounded) provided in the embodiment of the present application and fig. 8(b) a microfluidic chip with a top cover (grounded) in the prior art on a voltage observation line. Specifically, during simulation, control electrodes with the same area are adopted, one control electrode is connected, other control electrodes are grounded, and voltage change curves of two types of microfluidic chips with top covers on voltage observation lines under different numbers of grounded control electrodes are obtained by changing the number of the grounded control electrodes in the control electrode array. As shown in fig. 5, a voltage line can be chosen on the surface of dielectric layer 16 facing cap 25 (indicated by the arrows) where the voltage can determine whether droplet 42 can move. It can be seen that, with the increase of the number of the control electrodes, the voltage curves of the microfluidic chip with the top cover provided in the embodiment of the present application and the microfluidic chip with the top cover in the prior art on the voltage observation line are closer and closer, that is, the top cover voltage of the microfluidic chip with the top cover provided in the embodiment of the present application is closer and closer to the ground voltage with the increase of the number of the control electrodes. Therefore, it can be proved that the microfluidic chip with the top cover provided by the embodiment of the application can effectively realize the movement of the liquid drop when the total area of the grounded control electrodes is large enough. In addition, the microfluidic chip with the top cover provided by the embodiment of the application can control the bottom electrode to be grounded through the switch, the top cover does not need to be grounded, and the packaging difficulty is reduced.
In some embodiments, grounded peripheral electrodes may also be looped outside of electrode array 34 to increase the total area of the bottom grounded electrode. In practical tests, square electrode arrays with the side length of 1.39 mm and the side length of 1.90 mm are respectively designed for testing, and the electrode arrays are surrounded by grounded peripheral electrodes with larger areas. A single liquid drop is circularly moved between five electrodes in a single row, and the continuous moving success rate of the liquid drop in the state that the top cover is grounded and suspended is respectively researched by changing the moving time interval of each step. Along with the gradual increase of time interval, the continuous movement success rate of the liquid drops under the grounding and suspension states of the top cover is gradually improved. Experiments prove that in the electrode array with the side length of 1.39 mm, the continuous moving success rate of the liquid drops is 100% when the top cover is grounded and the time interval is 0.24 seconds, and the continuous moving success rate of the liquid drops is 100% when the top cover is suspended and the time interval is 0.26 seconds. In the electrode array with a side of 1.90 mm, the success rate of continuous movement of the droplets was 100% when the top cover was grounded and the time interval was 0.31 seconds, and the success rate of continuous movement of the droplets was 100% when the top cover was suspended and the time interval was 0.32 seconds. It can be demonstrated that the digital microfluidic system with the top cap suspended and the top cap grounded works approximately the same when the area of the peripheral ground electrode is large enough.
Referring to fig. 9, fig. 9 is a schematic cross-sectional structure view of a digital microfluidic system without a top cover according to an embodiment of the present disclosure. The digital microfluidic system without the top cover comprises a bottom electrode plate and a control circuit, wherein the bottom electrode plate comprises a hydrophobic layer 18, a dielectric layer 16 and a substrate 12 which are sequentially connected, and an electrode array 34 embedded between the dielectric layer 16 and the substrate 12. The control circuit includes a power supply 26 and a plurality of switches 38. The electrode array 34 includes a plurality of control electrodes, and the switches are connected to the control electrodes in a one-to-one correspondence. The switch has at least two connection positions for controlling the control electrode connected thereto to switch on the power supply 26 when the switch is in a first connection position and for controlling the control electrode connected thereto to be grounded when the switch is in a second connection position.
Referring to fig. 10, fig. 10 is a schematic diagram illustrating the operation of the microfluidic chip without the top cover shown in fig. 9. As shown in fig. 8, by setting the connection position of each switch, one control electrode (referred to as "on electrode") is turned on with the power source 26 to be at a high potential, and the other control electrode (referred to as "off electrode") is grounded to be at a low potential. A potential difference is created between the open and non-open electrodes that is large enough to cause droplet 42 to move. The micro-fluidic chip without the top cover provided by the embodiment of the application can control the bottom electrode to be grounded through the switch, and a smaller grounding electrode does not need to be additionally arranged between the electrodes connected with the switch, so that the packaging difficulty is reduced.
The above-mentioned embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same. Although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: modifications of the technical solutions described in the embodiments or equivalent replacements of some technical features may still be made. Such modifications and substitutions do not substantially depart from the spirit and scope of the embodiments of the present application and are intended to be included within the scope of the present application.
Claims (9)
1. A digital micro-fluidic system comprises a micro-fluidic chip and a control circuit, wherein the micro-fluidic chip comprises a bottom electrode plate, the bottom electrode plate comprises a plurality of electrodes, and the digital micro-fluidic system is characterized in that,
the plurality of electrodes comprise control electrodes arranged in an array, the control circuit comprises a plurality of switches, the switches are in one-to-one correspondence with the control electrodes, and each switch comprises a first connecting position connected to the ground and a second connecting position connected with a power supply.
2. The digital microfluidic system according to claim 1, wherein said switch comprises a reset selector and/or an optocoupler.
3. The digital microfluidic system of claim 1, wherein said power source comprises a signal generator, a voltage/level conversion device, or a power conversion device.
4. The digital microfluidic system according to claim 1, wherein said plurality of electrodes further comprises a ground electrode, said ground electrode being always grounded.
5. The digital microfluidic system according to claim 4, wherein said ground electrode surrounds the exterior of the control electrodes arranged in an array.
6. The digital microfluidic system of claim 1, wherein said microfluidic chip is a capless microfluidic chip.
7. The digital microfluidic system according to claim 1, wherein the microfluidic chip further comprises a top cover, a droplet moving space is formed between the top cover and the bottom electrode plate, the top cover comprises a top cover conductive layer, and the top cover conductive layer is in a voltage suspension state.
8. The digital microfluidic system according to claim 7, wherein the top cover conductive layer has a size not smaller than the size of the electrodes laid on the bottom electrode plate.
9. The digital microfluidic system of claim 1, wherein said power supply can provide a voltage signal or a current signal.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN112892625A (en) * | 2021-01-21 | 2021-06-04 | 京东方科技集团股份有限公司 | Micro-fluidic chip |
CN114582293A (en) * | 2022-03-10 | 2022-06-03 | 广东奥素液芯微纳科技有限公司 | Micro-fluidic active matrix driving circuit and micro-fluidic device |
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2019
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN112892625A (en) * | 2021-01-21 | 2021-06-04 | 京东方科技集团股份有限公司 | Micro-fluidic chip |
CN114582293A (en) * | 2022-03-10 | 2022-06-03 | 广东奥素液芯微纳科技有限公司 | Micro-fluidic active matrix driving circuit and micro-fluidic device |
CN114582293B (en) * | 2022-03-10 | 2023-08-04 | 广东奥素液芯微纳科技有限公司 | Microfluidic active matrix driving circuit and microfluidic device |
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Address after: 215300 Building 2, 1001 Yuyang Road, Yushan Town, Kunshan City, Suzhou City, Jiangsu Province Patentee after: Jiangsu drop logic Biotechnology Co.,Ltd. Address before: 215300 Unit 308, Biological Building 168 Yuanfeng Road, Kunshan High-tech Zone, Suzhou City, Jiangsu Province Patentee before: Jiangsu Aosu Liquid Core Biotechnology Co.,Ltd. |