CN211546526U - Cell factory digestion and suspension preparation device - Google Patents

Cell factory digestion and suspension preparation device Download PDF

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Publication number
CN211546526U
CN211546526U CN201922004895.7U CN201922004895U CN211546526U CN 211546526 U CN211546526 U CN 211546526U CN 201922004895 U CN201922004895 U CN 201922004895U CN 211546526 U CN211546526 U CN 211546526U
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cell factory
digestion
cell
support
rotary disk
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CN201922004895.7U
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陈韦韦
周琪
周鸿飞
周洁
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Luoyi Wuxi Biopharmaceutical Co ltd
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Luoyi Wuxi Biopharmaceutical Co ltd
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Abstract

The utility model relates to a cell mill digestion and suspension preparation technical field especially relate to a cell mill digestion and suspension preparation facilities, the on-line screen storage device comprises a base, the up end welding of base has the support, the horizontal part installation of support is fixed with the lantern ring, two siphunculus and three-way pipe have been welded respectively to the lower terminal surface of every lantern ring, the mouth of pipe of two siphunculus and three-way pipe all communicates there is the liquid pipe, the up end vertical welding of base has the telescopic link, and the up end horizontal welding of telescopic link pars contractilis has the layer board, the vertical welding of base up end has the rotary disk support, the upper portion of rotary disk support is rotated and is installed the rotary disk, the lug is all installed at one side of rotary disk. The utility model discloses in, not only improved the operating speed, reduced the error when a plurality of cell factory concurrent operation, cell factory imports and exports the position moreover and can not expose in the air to reduce the pollution risk, better satisfied the cultivation demand.

Description

Cell factory digestion and suspension preparation device
Technical Field
The utility model relates to a cell factory digestion and suspension preparation technical field especially relate to a cell factory digestion and suspension preparation facilities.
Background
Cell factory is a cell culture instrument that biology, pharmaceutical industry are commonly used, mainly be fit for the batch production of industrialization, for example bacterin, monoclonal antibody or pharmaceutical industry, the cell factory operation that prior art is commonly used carries out the liquid business turn over with simple pipe-line system utilization gravity poor mode usually, need change the connecting pipe many times during the use, not only operating speed is slow, it is easy to go out the error when a plurality of cell factories operate simultaneously, and the time that cell factory import and export position exposes in the air is longer, increase the pollution risk, can't be better satisfy the cultivation demand.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the defects existing in the prior art and providing a cell factory digestion and suspension preparation device.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a cell factory digestion and suspension preparation device comprises a base, wherein a support is welded on the upper end face of the base and comprises a horizontal portion and a vertical portion, lantern rings are fixedly installed on the horizontal portion of the support through screws, a two-way pipe and a three-way pipe are respectively welded on the lower end face of each lantern ring, pipe orifices of the two-way pipe and the three-way pipe are communicated with liquid pipes, a first throttle valve, a second throttle valve, a third throttle valve and a fourth throttle valve are sequentially installed on the liquid pipe between every two of the lantern rings from left to right, a telescopic rod is vertically welded on the upper end face of the base and comprises a fixed portion and a telescopic portion, a supporting plate is horizontally welded on the upper end face of the telescopic portion of the telescopic rod, a first cell factory and a second cell factory are sequentially placed on the upper end face of the supporting plate from left to right, a rotary disk support, the rotary disc is characterized in that a lug is fixedly arranged on one side of the rotary disc through a screw, a lug is fixedly arranged in the middle of one side of the supporting plate through a screw, and the lug on one side of the rotary disc and the lug in the middle of one side of the supporting plate are fixedly connected through a connecting rod.
Preferably, a cell suspension bottle, a waste liquid bottle, a digestion liquid bottle and a cleaning liquid bottle are sequentially arranged on the upper end face of the base from left to right.
Preferably, two through holes are formed in the upper ends of the cell suspension bottle, the waste liquid bottle, the digestion liquid bottle and the cleaning liquid bottle, a liquid pipe is communicated with one through hole, and one end of a suction pump is communicated with the other through hole.
Preferably, the upper ends of the first cell factory and the second cell factory are provided with two through holes, one through hole is communicated with a liquid pipe, the other through hole is communicated with one end of a suction pump, and each liquid pipe communicated with the inside of the cell factory is provided with a first check valve and a second check valve respectively.
Preferably, the telescopic part of the telescopic rod is sleeved with a spring, and the spring is positioned between the upper end face of the telescopic rod fixing part and the lower end face of the supporting plate.
Preferably, the upper end face of the supporting plate is fixedly provided with a clamping groove in a gluing mode, and the first cell factory and the second cell factory are respectively located in the clamping grooves.
Preferably, a motor is fixedly arranged on one side of the upper end of the rotating disc support, and a rotating disc is rotatably arranged at the output end of the motor through a coupling.
The utility model has the advantages that: the utility model discloses in, through installation two siphunculus and three-way pipe in this cell factory digestion and suspension preparation facilities, the mouth of pipe of two siphunculus and three-way pipe all communicates there is the liquid pipe, and all install the choke valve on the liquid pipe between per two siphunculus, install the check valve on the liquid pipe of two cell factories simultaneously, when using, operating speed has not only been improved, reduce the error when a plurality of cell factories operate simultaneously, and the cell factory imports and exports the position and can not expose in the air, thereby pollution risk has been reduced, better satisfied the cultivation demand.
Drawings
FIG. 1 is a schematic view of a cell factory digestion and suspension preparation device according to the present invention;
in the figure: the device comprises a base 1, a bracket 2, a lantern ring 3, a liquid pipe 4, a first throttle valve 51, a second throttle valve 52, a third throttle valve 53, a fourth throttle valve 54, a telescopic rod 6, a supporting plate 7, a spring 8, a clamping groove 9, a rotating disk bracket 10, a rotating disk 11, a connecting rod 12, a first check valve 131 and a second check valve 132.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
Referring to fig. 1, a cell factory digestion and suspension preparation device comprises a base 1, wherein a telescopic rod 6 is vertically welded on the upper end face of the base 1, the telescopic rod 6 comprises a fixed part and a telescopic part, a supporting plate 7 is horizontally welded on the upper end face of the telescopic part of the telescopic rod 6, a clamping groove 9 is fixed on the upper end face of the supporting plate 7 in a gluing manner, a first cell factory and a second cell factory are respectively positioned in the clamping groove 9, the cell factory slips off the supporting plate 7 when the cell factory is used, a spring 8 is sleeved on the telescopic part of the telescopic rod 6, the spring 8 is positioned between the upper end face of the fixed part of the telescopic rod 6 and the lower end face of the supporting plate 7, a certain buffering effect is generated when the cell factory vibrates, the first cell factory and the second cell factory are sequentially placed on the upper end face of the supporting plate 7 from left to right, two through holes are formed in the upper ends of the first cell factory and the second, the liquid pipe 4 is made of silica gel, one end of a suction pump is communicated with the other through hole, each liquid pipe 4 communicated with the inside of the cell factory is respectively provided with a first check valve 131 and a second check valve 132 which better react with liquid in the cell factory, the upper end surface of the base 1 is vertically welded with a rotary disk support 10, one side of the upper end of the rotary disk support 10 is fixedly provided with a motor, the output end of the motor is rotatably provided with a rotary disk 11 through a coupler, the upper part of the rotary disk support 10 is rotatably provided with a rotary disk 11, one side of the rotary disk 11 is fixedly provided with a convex block through a screw, the middle part of one side of the supporting plate 7 is fixedly provided with a convex block through a screw, the convex block on one side of the rotary disk 11 and the convex block on the middle part of one side of the supporting plate 7 are fixedly connected through a connecting rod 12, when the motor drives the rotary disk, better carry out the intensive mixing reaction to the liquid in the cell factory, the cell suspension bottle has been placed from a left side to turning right in proper order to base 1 up end, the waste liquid bottle, digestive juice bottle and cleaning solution bottle, the cell suspension bottle, the waste liquid bottle, two through-holes have all been seted up to the upper end of digestive juice bottle and cleaning solution bottle, a through-hole in-connection has liquid pipe 4, the one end that has the suction pump is connected to another through-hole in-connection, can be better carry out positive suction and reverse suction to the liquid in the bottle, convenient to use person operates, base 1's up end welding has support 2, support 2 includes horizontal part and vertical part, and the horizontal part of support 2 is fixed with lantern ring 3 through the screw installation, the lower terminal surface of every lantern ring 3 has welded respectively two siphunculus and three-way pipe, the material of two siphunculus and three-way pipe is stainless steel, the mouth of two siphunculus and three-way pipe all communicates liquid pipe 4, The second throttle valve 52, the third throttle valve 53 and the fourth throttle valve 54 control the throttle valves and the check valves according to use requirements, so that the operation speed is improved, errors are reduced when a plurality of cell factories operate simultaneously, and the inlet and outlet parts of the cell factories are not exposed in the air, so that the pollution risk is reduced, and the culture requirements are better met.
In this embodiment, when the digestion and suspension preparation device of the cell factory is used, before the operation starts, the first throttle valve 51, the second throttle valve 52, the third throttle valve 53, the fourth throttle valve 54, the first check valve 131 and the second check valve 132 are closed in sequence to be in the blocking state, the second throttle valve 52, the first check valve 131 and the second check valve 132 are opened, the power supply of the suction pump communicated with the waste liquid bottle is opened, the old culture liquid in the cell factory is sucked into the waste liquid bottle, then the second check valve 132 of the pipeline is blocked, the third throttle valve 53 and the fourth throttle valve 54 are opened, 0.02% EDTA-2Na cleaning liquid (30 +/-10 ml/layer) is added into the cell factory, the second throttle valve 52, the third throttle valve 53, the fourth throttle valve 54 and the first check valve 131 of the pipeline are blocked, the power supply of the motor is started to drive the rotating disk 11 to rotate, the rotating disc 11 and the connecting rod 12 drive the supporting plate 7 to reciprocate up and down along the telescopic rod 6 due to the crank-link mechanism, the power supply of the motor is closed after the cell factory is shaken for 4-6 times, the second throttle valve 52, the first check valve 131 and the second check valve 132 are opened, the washing solution is pumped into the waste liquid bottle, the second check valve 132 is blocked, the third throttle valve 53 is opened, the digestive juice (30 +/-10 ml/layer) is added into the cell factory, the second throttle valve 52, the third throttle valve 53 and the first check valve 131 are blocked, the power supply of the motor is turned on again, the motor drives the rotating disc 11 to rotate, the rotating disc 11 and the connecting rod 12 drive the supporting plate 7 to reciprocate up and down along the telescopic rod 6 due to the crank-link mechanism, the cell factory is shaken for 4-6 times, the digestive juice is fully soaked into the cell surface, the second throttle valve 52, the first check valve 131 and the second check valve 132 of the pipeline are, leaving a proper amount of digestive juice to continue digesting, opening the first throttle valve 51 and the first check valve 131 of the pipeline, pumping the digested cell sap into the cell suspension bottle to be fully mixed with the culture solution, pumping the cell suspension bottle and the culture solution into a cell factory according to the proportion, and controlling the throttle valve and the check valve according to the use requirement, thereby not only improving the operation speed and reducing the error when a plurality of cell factories are operated simultaneously, but also preventing the inlet and outlet parts of the cell factory from being exposed in the air, thereby reducing the pollution risk and better meeting the culture requirement.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims (7)

1. A cell factory digestion and suspension preparation device comprises a base (1) and is characterized in that a support (2) is welded on the upper end face of the base (1), the support (2) comprises a horizontal part and a vertical part, lantern rings (3) are fixedly arranged on the horizontal part of the support (2) through screws, a two-way pipe and a three-way pipe are respectively welded on the lower end face of each lantern ring (3), pipe orifices of the two-way pipe and the three-way pipe are communicated with a liquid pipe (4), a first throttle valve (51), a second throttle valve (52), a third throttle valve (53) and a fourth throttle valve (54) are sequentially installed on the liquid pipe (4) between every two through pipes from left to right, a telescopic rod (6) is vertically welded on the upper end face of the base (1), the telescopic rod (6) comprises a fixed part and a telescopic part, and a supporting plate (7) is horizontally welded on the upper end face of, first cell factory and second cell factory have been placed in proper order from a left side to right side to the up end of layer board (7), base (1) up end vertical welding has rotary disk support (10), the upper portion of rotary disk support (10) is rotated and is installed rotary disk (11), one side of rotary disk (11) is fixed with the lug through the mounting screw, the middle part of layer board (7) one side is fixed with the lug through the mounting screw, and be connected fixedly through connecting rod (12) between the lug of rotary disk (11) one side and the lug at layer board (7) one side middle part.
2. The cell factory digestion and suspension preparation device according to claim 1, characterized in that a cell suspension bottle, a waste liquid bottle, a digestion liquid bottle and a cleaning liquid bottle are sequentially arranged on the upper end face of the base (1) from left to right.
3. The cell factory digestion and suspension preparation device according to claim 2, wherein the upper ends of the cell suspension bottle, the waste liquid bottle, the digestion liquid bottle and the cleaning liquid bottle are all provided with two through holes, one through hole is communicated with the liquid tube (4), and the other through hole is communicated with one end of the suction pump.
4. The cell factory digestion and suspension preparation device according to claim 1, wherein the first cell factory and the second cell factory are each provided with two through holes, one through hole is connected with the liquid tube (4), the other through hole is connected with one end of the suction pump, and each liquid tube (4) connected with the inside of the cell factory is provided with the first check valve (131) and the second check valve (132).
5. The cell factory digestion and suspension preparation device according to claim 1, characterized in that the telescopic part of the telescopic rod (6) is sleeved with a spring (8), and the spring (8) is positioned between the upper end surface of the fixed part of the telescopic rod (6) and the lower end surface of the supporting plate (7).
6. The cell factory digestion and suspension preparation device according to claim 1, wherein the upper end face of the support plate (7) is fixed with a clamping groove (9) in a gluing way, and the first cell factory and the second cell factory are respectively positioned in the clamping groove (9).
7. The cell factory digestion and suspension preparation device according to claim 1, characterized in that a motor is fixed on one side of the upper end of the rotating disc support (10), and the output end of the motor is rotatably provided with a rotating disc (11) through a coupling.
CN201922004895.7U 2019-11-20 2019-11-20 Cell factory digestion and suspension preparation device Active CN211546526U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201922004895.7U CN211546526U (en) 2019-11-20 2019-11-20 Cell factory digestion and suspension preparation device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201922004895.7U CN211546526U (en) 2019-11-20 2019-11-20 Cell factory digestion and suspension preparation device

Publications (1)

Publication Number Publication Date
CN211546526U true CN211546526U (en) 2020-09-22

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Application Number Title Priority Date Filing Date
CN201922004895.7U Active CN211546526U (en) 2019-11-20 2019-11-20 Cell factory digestion and suspension preparation device

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114350517A (en) * 2022-01-14 2022-04-15 深圳赛动智造科技有限公司 Intelligent stem cell culture equipment based on cell factory and culture method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114350517A (en) * 2022-01-14 2022-04-15 深圳赛动智造科技有限公司 Intelligent stem cell culture equipment based on cell factory and culture method thereof

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