CN211522202U - Cell waste liquid suction filtration device for cell culture plate and cell culture bottle - Google Patents
Cell waste liquid suction filtration device for cell culture plate and cell culture bottle Download PDFInfo
- Publication number
- CN211522202U CN211522202U CN201922301846.XU CN201922301846U CN211522202U CN 211522202 U CN211522202 U CN 211522202U CN 201922301846 U CN201922301846 U CN 201922301846U CN 211522202 U CN211522202 U CN 211522202U
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- China
- Prior art keywords
- cell culture
- pipe
- rubber tube
- waste liquid
- cell
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- Expired - Fee Related
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 30
- 239000007788 liquid Substances 0.000 title claims abstract description 28
- 239000002699 waste material Substances 0.000 title claims abstract description 28
- 238000000967 suction filtration Methods 0.000 title claims abstract description 11
- 239000000725 suspension Substances 0.000 claims description 14
- 230000003068 static effect Effects 0.000 claims description 11
- 229910001220 stainless steel Inorganic materials 0.000 claims description 8
- 239000010935 stainless steel Substances 0.000 claims description 8
- 238000004804 winding Methods 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 238000005213 imbibition Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000012007 large scale cell culture Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a cell waste liquid suction filtration device for cell culture board and cell culture bottle, include: vacuum pump, suction flask, pipette and removal bear the base. The utility model has compact structure, simple device, convenient use and convenient operation, can remove a large amount of cell and tissue engineering waste liquid in a short time, and saves manpower and material resources; the user can be convenient the optional position department suction waste liquid on operation platform, need not to remove cell culture board and cell culture bottle, the accessible removes and bears the whole cell waste liquid suction filter device of base transfer, and the line storage function of operation panel makes the user can take out enough long pipelines in real time and carry out the imbibition, has better effect of accomodating simultaneously, avoids operation platform to go up the pipeline and is disorderly and have no chapter, influences the operation.
Description
Technical Field
The utility model belongs to the technical field of used equipment in biological laboratory, especially, relate to a cell waste liquid suction filtration device for cell culture board and cell culture bottle.
Background
The frequent replacement of the culture solution is the basic requirement for the healthy growth of cells, so that a large amount of cell waste liquid can be generated in large-scale cell culture, and if the cell waste liquid can be effectively and quickly absorbed, the experimental time can be greatly saved, and the working efficiency can be improved. At present, the cell culture plate and the cell culture bottle absorb waste liquid mainly through a liquid-transferring discharging gun or a liquid-transferring device to repeatedly absorb and discharge the waste liquid, and the process is time-consuming and labor-consuming and also cost money.
In order to solve the above technical problems, those skilled in the art have proposed some devices for aspirating cell waste liquid, and these devices have insufficient flexibility and cannot be simultaneously applied to cell culture bottles, culture dishes and culture plates of various specifications. In addition, the existing device for sucking and removing the cell waste liquid needs to frequently move equipment to match the position of a cell culture plate or a cell culture bottle when in use, and has complex operation and inconvenient operation.
Disclosure of Invention
In order to solve the technical problem, the utility model provides a cell waste liquid suction filtration device for cell culture plate and cell culture bottle. The following presents a simplified summary in order to provide a basic understanding of some aspects of the disclosed embodiments. This summary is not an extensive overview and is intended to neither identify key/critical elements nor delineate the scope of such embodiments. Its sole purpose is to present some concepts in a simplified form as a prelude to the more detailed description that is presented later.
The utility model adopts the following technical scheme:
in some alternative embodiments, there is provided a cell waste filtration device for cell culture plates and bottles, comprising: the device comprises a vacuum pump, a suction bottle, a pipette and a movable bearing base, wherein the vacuum pump and the suction bottle are arranged on the movable bearing base; the mobile load-bearing base comprises: the suspension bracket is arranged on the chassis, the filter flask is mounted on the suspension bracket, and the operating panel is connected with the suspension bracket through a spiral spring; a wire winding groove is formed in the operating disc, a rubber tube wiring channel is formed in the wire winding groove, a wiring pipe fitting is arranged at the outlet of the rubber tube wiring channel, the pipette is communicated with one end of a first rubber tube, the first rubber tube is wound on the operating disc, the other end of the first rubber tube is communicated with the wiring pipe fitting, and the wiring pipe fitting is communicated with the suction bottle through a second rubber tube; the pipette is provided with a needle receiving port, and the needle frame is provided with a plurality of stainless steel suction needles with different lengths.
In some optional embodiments, the junction block includes: the device comprises a pipe shell, an action pipe and a static pipe, wherein the action pipe is communicated with the first rubber pipe, and the static pipe is communicated with the second rubber pipe; the end part of the action pipe is provided with a groove, the end part of the static pipe is provided with a lug which is matched with the groove, the action pipe extends into the static pipe, and the lug is embedded into the groove.
In some optional embodiments, a hanging platform is arranged on the hanging frame, the hanging platform is provided with a hanging port, a fastening ring is arranged on the surface of the filter flask, and a hanging lug matched with the hanging port is arranged on the fastening ring.
In some optional embodiments, a plurality of arc-shaped mounting grooves are further formed in the mounting platform, and the pipettes are mounted on the arc-shaped mounting grooves.
In some optional embodiments, the filter flask is connected to the vacuum pump through a third rubber tube, and a water blocking filter is disposed on the third rubber tube.
In some optional embodiments, the cell waste solution suction filtration device for cell culture plates and cell culture flasks further comprises: a controller for controlling the vacuum pump; the controller is arranged on the mobile bearing base.
In some optional embodiments, the stainless steel suction needle is screwed with the needle receiving port of the pipette.
The utility model discloses the beneficial effect who brings: through the design of the movable bearing base and the operation panel, a user can conveniently suck waste liquid at any position on the operation platform without moving a cell culture plate and a cell culture bottle, the whole cell waste liquid suction filtration device can be transferred by moving the movable bearing base, and the wire storage function of the operation panel enables the user to draw out a pipeline with enough length in real time for liquid suction, so that the wire suction filtration device has a good storage effect, and avoids the phenomenon that the pipeline on the operation platform is disordered and the operation is influenced; the utility model discloses compact structure, the device is simple, and convenient to use, the operation of being convenient for can remove a large amount of cells and tissue engineering waste liquid in the short time, the material resources of using manpower sparingly.
Drawings
FIG. 1 is a schematic structural view of the cell waste liquid suction filtration device of the present invention in a storage state;
FIG. 2 is a schematic diagram of the operation panel according to the present invention;
fig. 3 is a schematic position diagram of the operation panel, the suspension bracket and the wiring pipe fitting of the present invention;
FIG. 4 is a schematic structural view of the mounting platform of the present invention;
fig. 5 is a schematic structural view of the junction box of the present invention.
Detailed Description
The following description and the drawings sufficiently illustrate specific embodiments of the invention to enable those skilled in the art to practice them. Other embodiments may incorporate structural, logical, electrical, process, and other changes. The examples merely typify possible variations. Individual components and functions are optional unless explicitly required, and the sequence of operations may vary. Portions and features of some embodiments may be included in or substituted for those of others.
In some illustrative embodiments, as shown in fig. 1 to 3, there is provided a cell waste suction filtration device for cell culture plates and cell culture flasks, comprising: vacuum pump 1, buchner flask 2, pipette 3, remove and bear base 4, first rubber tube 5, second rubber tube 6, third rubber tube 7, wiring pipe fitting 8, filter 9 and controller 10 block water.
Vacuum pump 1, buchner flask 2, wiring pipe fitting 8 and controller 10 set up on removing bearing base 4, and the bottom that removes bearing base 4 can set up the travelling wheel, conveniently shifts and removes bearing base 4.
The mobile bearing base 4 comprises: chassis 41, hanger 42, operating panel 43 and needle frame 44. A suspension bracket 42 is provided on the base plate 41, an operating plate 43 is connected to the suspension bracket 42 by an axle so that the operating plate 43 can rotate on the suspension bracket 42, and the suspension bracket 42 is connected to the operating plate 43 by a volute spring. The spiral spring is a plane spiral spring, also called a clockwork spring, and is a spring with one fixed end and the other end acted with torque; the spring material is elastically deformed by bending under the action of the torque, so that the spring is twisted in a plane, and therefore, how to enable the operating panel 43 to have the tendency of resetting on the suspension bracket 42 is realized by those skilled in the art, and the present invention is not described in detail again.
As shown in fig. 2, a wire winding groove 45 is formed in the operating panel 43, a hose routing channel 46 is formed in the wire winding groove 45, and the outlet of the hose routing channel 46 is provided with the wiring pipe 8. First rubber tube 5 twines on operation panel 43 and the one end and the pipette 3 intercommunication of first rubber tube 5, the other end around wire winding groove 45 after with the junction box 8 intercommunication. The wiring pipe fitting 8 is communicated with the suction flask 2 through the second rubber tube 6, and the suction flask 2 is connected with the vacuum pump 1 through the third rubber tube 7. In operation, the user takes off pipette 3 from the hanger bracket 42, pulls the first rubber tube 5, overcomes the restoring force of the spiral spring and draws out the first rubber tube 5 to take out liquid, when the pipette 3 is not needed, the pulling force is reduced, and at the moment, the first rubber tube 5 is gradually wound on the operating plate 43 under the acting force of the spiral spring. The vacuum pump 1 works to suck air in the suction filter flask 2 through the third rubber tube 7 to form negative pressure, so that the sucked waste liquid enters the suction filter flask 2 through the first rubber tube 5 and the second rubber tube 6.
The pipette 3 is provided with a needle receiving port 31, and the needle frame 44 is provided with a plurality of stainless steel suction needles 11 with different lengths. The stainless steel suction needle 11 is connected with the needle connecting port 31 of the pipette through threads, when a cell culture plate or a cell culture bottle absorbs waste liquid, the stainless steel suction needle 11 with different lengths is selected, when different cell waste liquid is absorbed, the stainless steel suction needle 11 is replaced, and the use is convenient and convenient.
Preferably, a water blocking filter 9 is arranged on the third rubber tube 7 to prevent back suction.
The filter flask 2 is mounted on the suspension bracket 42. As shown in fig. 4, the hanging frame 42 is provided with a hanging platform 47, the hanging platform 47 is provided with a hanging port 49, the surface of the filter flask 2 is provided with a fastening ring 21, the fastening ring 21 is provided with a hanging lug 22 matched with the hanging port, when the filter flask 2 is hung on the hanging frame 42, the hanging lug 22 is embedded into the hanging port, the filter flask 2 can be taken at any time, and the use is convenient. Still set up a plurality of arc carry grooves 48 on the carry platform 47, pipette 3 carries on arc carry groove 48, sets up the stopper on pipette 3, effectively avoids pipette 3 to drop.
As shown in fig. 5, the junction block 8 includes: the tube housing 81, the operating tube 82, and the stationary tube 83, the operating tube 82 communicating with the first rubber tube 5, and the stationary tube 83 communicating with the second rubber tube 6. Recess 84 is seted up to the tip of action pipe 82, the tip of static pipe 83 sets up the lug 85 with recess 84 adaptation, action pipe 82 stretches into in static pipe 83 and lug 85 embedding recess 84, consequently, when action pipe 82 is driven by operation panel 43 and is rotated, its tip is at static intraductal 83 internal rotation, and do not influence the flow of waste liquid, avoid leading to first rubber tube 5 and the unable mobile problem of liquid that results in of second rubber tube 6 wrench movement winding because operation panel 43 rotates, as long as first rubber tube 5 from the rubber tube wiring passageway 46 draw forth the back vertically be connected with wire connection pipe fitting 8 can. In order to facilitate the rotation of the operation tube 82, a ball may be provided between the operation tube 82 and the stationary tube 83, and a gasket may be provided between the operation tube 82 and the stationary tube 83 in order to prevent the waste liquid from leaking.
The controller 10 is used for controlling the vacuum pump 1, such as controlling the on and off of the vacuum pump 1, the vacuum rate, etc., and the controller 10 is disposed on the mobile carrying base 4.
The above embodiment is the preferred embodiment of the present invention, but the embodiment of the present invention is not limited by the above embodiment, and any other changes, modifications, replacements, combinations, simplifications, equivalent replacement modes, which are not departed from the spirit and principle of the present invention, should be included in the protection scope of the present invention.
Claims (7)
1. A cell waste liquid suction filter device for cell culture board and cell culture bottle, its characterized in that includes: the device comprises a vacuum pump, a suction bottle, a pipette and a movable bearing base, wherein the vacuum pump and the suction bottle are arranged on the movable bearing base; the mobile load-bearing base comprises: the suspension bracket is arranged on the chassis, the filter flask is mounted on the suspension bracket, and the operating panel is connected with the suspension bracket through a spiral spring; a wire winding groove is formed in the operating disc, a rubber tube wiring channel is formed in the wire winding groove, a wiring pipe fitting is arranged at the outlet of the rubber tube wiring channel, the pipette is communicated with one end of a first rubber tube, the first rubber tube is wound on the operating disc, the other end of the first rubber tube is communicated with the wiring pipe fitting, and the wiring pipe fitting is communicated with the suction bottle through a second rubber tube; the pipette is provided with a needle receiving port, and the needle frame is provided with a plurality of stainless steel suction needles with different lengths.
2. The apparatus of claim 1, wherein the connector comprises: the device comprises a pipe shell, an action pipe and a static pipe, wherein the action pipe is communicated with the first rubber pipe, and the static pipe is communicated with the second rubber pipe; the end part of the action pipe is provided with a groove, the end part of the static pipe is provided with a lug which is matched with the groove, the action pipe extends into the static pipe, and the lug is embedded into the groove.
3. The device as claimed in claim 2, wherein the suspension frame is provided with a mounting platform, the mounting platform is provided with a mounting opening, the surface of the filtration flask is provided with a fastening ring, and the fastening ring is provided with a lug adapted to the mounting opening.
4. The apparatus of claim 3, wherein the mounting platform further comprises a plurality of arc-shaped mounting grooves, and the pipette is mounted on the arc-shaped mounting grooves.
5. The apparatus of claim 4, wherein the filtration flask is connected to the vacuum pump via a third rubber tube, and the third rubber tube is provided with a water-blocking filter.
6. The apparatus for suction filtration of cell waste liquid used for cell culture plates and cell culture flasks as claimed in any one of claims 1 to 5, further comprising: a controller for controlling the vacuum pump; the controller is arranged on the mobile bearing base.
7. The apparatus according to claim 6, wherein the stainless steel suction needle is screwed to the needle receiving port of the pipette.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922301846.XU CN211522202U (en) | 2019-12-19 | 2019-12-19 | Cell waste liquid suction filtration device for cell culture plate and cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922301846.XU CN211522202U (en) | 2019-12-19 | 2019-12-19 | Cell waste liquid suction filtration device for cell culture plate and cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211522202U true CN211522202U (en) | 2020-09-18 |
Family
ID=72466156
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922301846.XU Expired - Fee Related CN211522202U (en) | 2019-12-19 | 2019-12-19 | Cell waste liquid suction filtration device for cell culture plate and cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211522202U (en) |
-
2019
- 2019-12-19 CN CN201922301846.XU patent/CN211522202U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200918 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |