CN211505563U

CN211505563U - Furaltadone metabolite rapid detection card

Info

Publication number: CN211505563U
Application number: CN201922369066.9U
Authority: CN
Inventors: 盛相国
Original assignee: Suzhou Kuaijiekang Biotechnology Co ltd
Current assignee: Suzhou Kuaijiekang Biotechnology Co ltd
Priority date: 2019-12-25
Filing date: 2019-12-25
Publication date: 2020-09-15
Anticipated expiration: 2029-12-25

Abstract

The utility model relates to a furaltadone metabolite rapid detection card, which comprises a PVC plate, a sample pad, a colloidal gold combined pad, a nitrocellulose membrane and a water absorption pad, and also comprises a shell and a sample adding mechanism, wherein the shell is provided with an observation part, the sample adding mechanism comprises a fixed tube, a sample adding component, a sample adding port, a sealing component and a fixed component, the sample adding component comprises a cross rod, a vertical rod, a sealing plug and a sealing ring, two sides of the upper part of the sample adding tube are respectively provided with a side plate and a spring, the furaltadone metabolite rapid detection card realizes the protection of the sample pad, the colloidal gold combined pad, the nitrocellulose membrane and the water absorption pad through the shell, and the sample solution is conveniently and gradually added into the sample pad by the sample adding mechanism, and prevents the sample liquid from flowing to other positions to pollute clothes or skin of a tester in the sample adding process, the flow of the sample liquid is limited, so that the equipment operation is more convenient and safer, and the practicability of the detection card is improved.

Description

Furaltadone metabolite rapid detection card

Technical Field

The utility model relates to the technical field of bioengineering, in particular to furaltadone metabolite short-term test card.

Background

At present, methods for detecting furaltadone metabolites (AMOZ) mainly comprise microbiological methods, chromatographic methods, immunoassay methods and the like. The microbiological method is a classical method for determining antibiotic residues, has the advantages of simple pretreatment, economy, large batch and the like, but has low accuracy, accuracy and specificity. The chromatography is more generally applied, and has high accuracy and sensitivity, but the field detection and the popularization are difficult to realize due to the reasons of complicated flow, expensive equipment, low detection speed and the like. The enzyme-linked immunosorbent assay is based on antigen-antibody reaction, is accurate and sensitive, and greatly shortens the detection time, but the activity of enzyme in the enzyme-linked immunosorbent assay is easily influenced by reaction conditions, so that the repeatability of a detection result is poor. Furthermore, ELISA reagents have a short life and therefore require cryopreservation. The immunological determination method established based on antigen-antibody specific reaction has the advantages of high sensitivity, strong specificity, simple sample pretreatment, short analysis time and convenient use. Thus, immunological detection methods are more practical in field monitoring and basic large-scale screening assays.

The colloidal gold immunochromatography is a simple and rapid immunological detection technology established on the basis of an immunodiafiltration technology, and overcomes some defects of an enzyme-linked immunosorbent assay besides the advantages of the enzyme-linked immunosorbent assay. The method integrates all or most of raw materials required by the reaction into the reagent, the reaction usually only needs a few minutes, the test result is judged by a macroscopic color development strip, and the method has the advantages of simplicity, convenience, rapidness, simple operation, strong specificity, no need of additional equipment and the like. The furaltadone metabolite rapid detection card is a detection device designed based on the detection method.

The existing furaltadone metabolite rapid detection card is exposed outside, is easily polluted and damaged, influences the use, in the use process, a proper amount of sample is required to be accurately dripped on a sample pad part of the detection card, such operation has higher requirements on detection personnel, when the detection is carried out, the sample liquid is easily added too much, the sample liquid can be instantly poured to a detection area, a false negative effect is generated, even the failure is caused, when the sample liquid is added, the residual sample liquid at the sample adding part is easy to flow to other positions when the detection card is taken to observe a detection result, the pollution detection card, clothes or skin of the detection personnel is polluted, and the practicability of the existing furaltadone metabolite rapid detection card is reduced.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is: in order to overcome the defects of the prior art, the furaltadone metabolite rapid detection card is provided.

The utility model provides a technical scheme that its technical problem adopted is: a furaltadone metabolite rapid detection card comprises a PVC (polyvinyl chloride) plate, a sample pad, a colloidal gold combined pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad, the colloidal gold combined pad, the nitrocellulose membrane and the water absorption pad are sequentially fixed above the PVC plate, the nitrocellulose membrane is provided with a detection line and a quality control line, the furaltadone metabolite rapid detection card further comprises a shell and a sample adding mechanism, the shell is fixed above the PVC plate, the sample pad, the colloidal gold combined pad, the nitrocellulose membrane and the water absorption pad are all positioned on the inner side of the shell, the sample adding mechanism is positioned above the sample pad, the shell is provided with an observation part, the shell is made of transparent plastic, and the observation part is positioned above the nitrocellulose membrane;

the sample adding mechanism comprises a fixed tube, a sample adding assembly, a sample adding port, a sealing assembly and a fixed assembly, the sample adding port is arranged on the shell, the fixed tube is fixed at the top in the shell, the periphery of the sample adding tube is hermetically connected with the inner wall of the sample adding port, the sample adding assembly is positioned at the inner side of the fixed tube, the sample adding assembly comprises a transverse rod, a vertical rod, a sealing plug and a sealing ring, the periphery of the sealing ring is fixed on the inner wall of the fixed tube, the two ends of the transverse rod are fixed on the inner wall of the sample adding tube, the sealing plug is fixed below the transverse rod through the vertical rod, the vertical rod is positioned at the inner side of the sample adding port, the two sides of the upper part of the sample adding tube are respectively provided with a side plate and a spring, the side plates are fixedly connected with the sample adding tube, the two ends of the spring are, the sealing assembly comprises a cover plate and a rotating unit, the cover plate is arranged on the top end of the sample adding pipe in a covering mode, the cover plate is connected with one side plate through the rotating unit, and the fixing assembly is arranged at one end, far away from the rotating unit, of the cover plate.

Preferably, the observation portion is an upwardly convex structure in order to facilitate observation of the detection result.

Preferably, in order to prevent the sample liquid from being added to the outside, the end of the sample addition tube remote from the fixed tube is flared.

Preferably, in order to facilitate the rotation of the cover plate, the rotating unit comprises a sleeve ring, a convex plate and a rotating rod, the sleeve ring is fixed on the side rod, the convex plate is fixed below the cover plate through the rotating rod, and the sleeve ring is sleeved on the rotating rod.

As preferred, in order to facilitate the position of fixed apron and application of sample pipe, fixed subassembly includes fixed block, pivot and two splint, the shape of the vertical cross-section of fixed block is the U-shaped, the one end of keeping away from the rotation unit of apron is located the U-shaped opening of fixed block, the fixed block is located the top of pivot, and two splint are located the top of casing and the top in the casing respectively, splint are fixed in the pivot.

Preferably, in order to ensure the sealing performance between the sealing plug and the sealing ring after the sample adding pipe moves upwards, the sealing plug is in the shape of a conical cylinder, the outer diameter of the top of the sealing plug is smaller than the inner diameter of the sealing ring, and the outer diameter of the bottom of the sealing plug is larger than the inner diameter of the sealing ring.

The beneficial effects of the utility model are that, this furaltadone metabolite short-term test cartoon passes through the casing and realizes combining the protection of filling up, cellulose nitrate membrane and the pad that absorbs water to sample pad, colloidal gold, utilizes application of sample mechanism convenience to progressively add the appearance liquid to sample pad to avoid application of sample in-process appearance liquid to flow to other positions and pollute measurement personnel's clothing or skin, through the flow of restriction appearance liquid, make the equipment operation more convenient safety, thereby improved this detection card's practicality.

Drawings

The present invention will be further explained with reference to the drawings and examples.

FIG. 1 is a schematic structural diagram of a furaltadone metabolite rapid detection card of the present invention;

FIG. 2 is an enlarged view of portion A of FIG. 1;

in the figure: the test device comprises a PVC plate 1, a sample pad 2, a colloidal gold combined pad 3, a nitrocellulose membrane 4, a water absorption pad 5, a test line 6, a quality control line 7, a shell 8, a clamping plate 9, an observation part 10, a fixing pipe 11, a sample adding pipe 12, a cross rod 13, a vertical rod 14, a sealing plug 15, a sealing ring 16, a sealing ring 17, a side plate 18, a spring 19, a cover plate 19, a lantern ring 20, a convex plate 21, a rotating rod 22, a fixing block 23 and a rotating shaft 24.

Detailed Description

The present invention will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic drawings and illustrate the basic structure of the present invention only in a schematic manner, and thus show only the components related to the present invention.

As shown in fig. 1, a furaltadone metabolite rapid detection card comprises a PVC plate 1, a sample pad 2, a colloidal gold combined pad 3, a nitrocellulose membrane 4 and a water absorption pad 5, wherein the sample pad 2, the colloidal gold combined pad 3, the nitrocellulose membrane 4 and the water absorption pad 5 are sequentially fixed above the PVC plate 1, the nitrocellulose membrane 4 is provided with a detection line 6 and a quality control line 7, the furaltadone metabolite rapid detection card further comprises a shell 8 and a sample adding mechanism, the shell 8 is fixed above the PVC plate 1, the sample pad 2, the colloidal gold combined pad 3, the nitrocellulose membrane 4 and the water absorption pad 5 are all located on the inner side of the shell 8, the sample adding mechanism is located above the sample pad 2, the shell 8 is provided with an observation part 10, the shell 8 is made of transparent plastic, and the observation part 10 is located above the nitrocellulose membrane 4;

in this furaltadone metabolite short-term test card, through setting up sample pad 2, colloidal gold combination pad 3, nitrocellulose membrane 4 and absorbent pad 5 in the inboard of casing 8, realize protect function, receive the pollution and damage when avoiding detecting the card flat, when using this detection card, can be with equipment square back, utilize application of sample mechanism to add the sample liquid on sample pad 2, because the preparation of sample liquid is the conventional means of this branch of academic or vocational study technique, the event is not repeated here. Sample liquid adds the back that finishes, utilizes application of sample mechanism still can realize sealing, prevents among the testing process, and sample liquid flows the outside for sample liquid pollutes other positions or measurement personnel's clothing or skin, and casing 8 adopts transparent plastic to make, and the convenience is detecting the back that finishes, observes the testing result through observation portion 10, and here, the judgement standard of testing result is:

negative: the color development ratio quality control line 7 of the detection line 6 is deep or the same deep, which indicates that the concentration of the furaltadone metabolite in the sample liquid is lower than the detection limit or does not contain the furaltadone metabolite;

positive: the detection line 6 is lighter than the quality control line 7 in color development, or the detection line 6 is not colored, which indicates that the furaltadone metabolite in the sample solution is larger than the detection limit, and the lighter the quality control line 7 in color development of the detection line 6 is, the larger the color is, which indicates that the residual concentration of the furaltadone metabolite in the sample solution is higher;

and (4) invalidation: the quality control line 7 does not develop color and may be mishandled or the test card fails, at which time the test needs to be performed again with a new test card.

As shown in fig. 2, the sample adding mechanism includes a fixed tube 11, a sample adding tube 12, a sample adding assembly, a sample adding port, a sealing assembly and a fixed assembly, the sample adding port is disposed on the housing 8, the fixed tube 11 is fixed on the top of the housing 8, the periphery of the sample adding tube 12 is connected with the inner wall of the sample adding port in a sealing manner, the sample adding assembly is located on the inner side of the fixed tube 11, the sample adding assembly includes a cross rod 13, a vertical rod 14, a sealing plug 15 and a sealing ring 16, the periphery of the sealing ring 16 is fixed on the inner wall of the fixed tube 11, two ends of the cross rod 13 are fixed on the inner wall of the sample adding tube 12, the sealing plug 15 is fixed below the cross rod 13 through the vertical rod 14, the vertical rod 14 is located on the inner side of the sample adding port, two sides of the upper portion of the sample adding tube 12 are both provided with a side plate 17 and a spring 18, the side plate 17 is fixedly, the spring 18 is in a compression state, the sealing assembly is connected with one of the side plates 17, the sealing assembly comprises a cover plate 19 and a rotating unit, the cover plate 19 is arranged at the top end of the sample adding pipe 12 in a covering mode, the cover plate 19 is connected with one of the side plates 17 through the rotating unit, and the fixing assembly is arranged at one end, far away from the rotating unit, of the cover plate 19.

In application of sample mechanism, conveniently rotate apron 19 to the top that covers at application of sample pipe 12 through rotating the unit to utilize the fixed position of apron 19 of fixed unit, thereby fixed application of sample pipe 12's position makes application of sample pipe 12 be close to fixed pipe 11 and removes, reduces the size of equipment, makes things convenient for equipment to store the transportation. When the detection card is used for detection, the cover plate 19 is rotated to one side far away from the sample adding pipe 12 through the rotating unit, at the moment, the compressed spring 18 is deformed to restore and pushes the side rod to move away from the shell 8, so that the sample adding pipe 12 moves away from the fixed pipe 11 and drives the cross rod 13 inside the sample adding pipe 12 to move away from the sample pad 2, the vertical rod 14 drives the sealing plug 15 to move until the sealing plug 15 abuts against the inner side of the sealing ring 16, the position of the sample adding pipe 12 is fixed at the moment, meanwhile, the sealing plug 15 and the sealing ring 16 block the inside of the fixed pipe 11, then, sample liquid can be added into the sample adding pipe 12, so that the sample liquid falls above the sealing plug 15 and the sealing ring 16, as the sample adding pipe 12 moves upwards in front, more sample liquid can be contained between the sample adding pipe 12 and the fixed pipe 11, the situation that the sample liquid flows to the outside when the sample liquid is added, make the apron 19 cover on adding the appearance pipe 12, after stopping up adding the appearance pipe 12, press the apron 19 close to the casing 8, thereby make adding the appearance pipe 12 drive the side lever and be close to the sample pad 2 and move, when compressing spring 18, drive horizontal pole 13 and move, make horizontal pole 13 drive sealing plug 15 and move, break away from the inboard of sealing ring 16, make the center department of sealing plug 15 open, at this moment, the appearance liquid in the fixed pipe 11 flows downwards through the inboard of sealing ring 16, fall on the sample pad 2, then loosen apron 19, the spring 18 that is compressed is for recovering deformation, promote the side lever to keep away from the casing 8 and move, thereby through adding the appearance pipe 12 and moving, drive sealing plug 15 and move, get into the inboard of sealing ring 16, stop up sealing ring 16, prevent the appearance liquid from continuing to contact with sample pad 2 through sealing ring 16, if need increase the appearance liquid volume that contacts with sample pad 2, the accessible is pressed apron 19 repeatedly, make when pressing at every turn, all have a certain amount of appearance liquid to flow to sample pad 2 through the sealing ring 16 inside on, simultaneously because apron 19 plugs up sample adding pipe 12 this moment, when the measurement personnel took up the detection card and observe, can avoid appearance liquid to flow the outside, pollute measurement personnel's clothing or skin, guarantee to detect and go on safely, so, improved the practicality of this detection card.

Preferably, the observation portion 10 is formed to be convex upward for the convenience of observing the detection result. After the observation part 10 protrudes upwards, the observation part can perform a light condensation effect, so that the display effect of the detection line 6 and the quality control line 7 on the nitrocellulose membrane 4 below is enhanced, and the observation is convenient for a detector to observe.

Preferably, in order to prevent the sample liquid from being added to the outside, the end of the sample addition tube 12 remote from the fixed tube 11 is flared. Adopt the design of tubaeform, increased the shape of the one end of keeping away from fixed pipe 11 of pressurization pipe, be convenient for add the appearance liquid, flow to the outside when preventing the appearance liquid from adding.

Preferably, in order to facilitate the rotation of the cover plate 19, the rotating unit comprises a collar 20, a convex plate 21 and a rotating rod 22, the collar 20 is fixed on the side rod, the convex plate 21 is fixed below the cover plate 19 through the rotating rod 22, and the collar 20 is sleeved on the rotating rod 22. The lantern ring 20 fixedly connected with the side rod is utilized, so that the rotating rod 22 can rotate conveniently, the cover plate 19 can be driven to rotate conveniently, the relative position of the lantern ring 20 and the rotating rod 22 is limited through the convex plate 21, and the rotating rod 22 is prevented from being separated from the lantern ring 20.

As preferred, in order to facilitate the position of fixed apron 19 and application of sample pipe 12, fixed subassembly includes fixed block 23, pivot 24 and two splint 9, the shape of the vertical cross-section of fixed block 23 is the U-shaped, the one end of keeping away from the rotation unit of apron 19 is located the U-shaped opening of fixed block 23, fixed block 23 is located the top of pivot 24, and two splint 9 are located the top in the top of casing 8 and casing 8 respectively, splint 9 are fixed on pivot 24.

The relative position of pivot 24 and casing 8 has been restricted through two splint 9, be convenient for pivot 24 rotates round self axis, drive fixed block 23 and rotate, the one end of keeping away from the rotation unit of apron 19 is located behind the U-shaped opening of fixed block 23, can fix the position of apron 19, thereby the position of fixed application of sample pipe 12, when rotating fixed block 23 and pivot 24, make the U-shaped opening of fixed block 23 no longer towards apron 19 after, the spring 18 that is compressed this moment promotes the side lever, make application of sample pipe 12 keep away from fixed pipe 11 and remove, support until sealing plug 15 and lean on sealing ring 16.

Preferably, in order to ensure the sealing performance between the sealing plug 15 and the sealing ring 16 after the loading tube 12 moves upwards, the sealing plug 15 is in the shape of a conical cylinder, the outer diameter of the top of the sealing plug 15 is smaller than the inner diameter of the sealing ring 16, and the outer diameter of the bottom of the sealing plug 15 is larger than the inner diameter of the sealing ring 16. By adopting the shape design, when the sealing plug 15 moves upwards, one end of the sealing plug 15 far away from the sample pad 2 extends into the inner side of the sealing ring 16, and the conical cylindrical surface of the sealing plug 15 abuts against the inner wall of the sealing ring 16, so that a good sealing effect on the sealing ring 16 is realized, and the added sample liquid is prevented from falling on the sample pad 2.

In the furaltadone metabolite detection card, the sample pad 2, the colloidal gold combined pad 3, the nitrocellulose membrane 4 and the water absorption pad 5 are protected by the shell 8, pollution damage is prevented, the position of the cover plate 19 is fixed by the fixing component, the sample adding pipe 12 is convenient to be close to the sample pad 2, the size of the device is reduced, and storage and transportation are convenient, when the furaltadone metabolite detection card is used, the cover plate 19 is rotated to be separated from the fixing block 23 and the pressure pipe, the compressed spring 18 pushes the sample adding pipe 12 to move until the sealing plug 15 blocks the sealing ring 16, then sample liquid can be added into the sample adding pipe 12, the cover plate 19 is rotated to be covered on the sample adding pipe 12, the sample adding pipe 12 moves downwards by pressing the cover plate 19 to drive the sealing plug 15 to be separated from the sealing ring 16, the sample liquid above the sealing ring 16 falls on the sample pad 2 through the inner side of the sealing ring 16, then the cover plate 19 is, utilize the spring 18 promotion of compression to add appearance pipe 12 and remove, drive sealing plug 15 and lean on to seal ring 16 inboard, prevent to continue to add appearance liquid, simultaneously at the application of sample in-process, apron 19 blocks up adding appearance pipe 12, avoids appearance liquid to flow to other positions, pollutes measurement personnel's clothing and skin, utilizes observation portion 10 to conveniently observe the color development result of detection line 6 and quality control line 7 to accomplish and detect.

Compared with the prior art, this furaltadone metabolite short-term test card passes through casing 8 and realizes the protection to sample pad 2, colloidal gold combination pad 3, nitrocellulose membrane 4 and absorbent pad 5, utilizes application of sample mechanism to make things convenient for progressively to add the sample liquid to sample pad 2 to avoid application of sample in-process sample liquid to flow other positions and pollute measurement personnel's clothing or skin, through the flow of restriction sample liquid, make equipment operation more convenient safety, thereby improved this detection card's practicality.

In light of the foregoing, it will be apparent to those skilled in the art from this disclosure that various changes and modifications can be made without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.

Claims

1. A furaltadone metabolite rapid detection card comprises a PVC plate (1), a sample pad (2), a colloidal gold combined pad (3), a nitrocellulose membrane (4) and a water absorption pad (5), wherein the sample pad (2), the colloidal gold combined pad (3), the nitrocellulose membrane (4) and the water absorption pad (5) are sequentially fixed above the PVC plate (1), the nitrocellulose membrane (4) is provided with a detection line (6) and a quality control line (7), the furaltadone metabolite rapid detection card is characterized by further comprising a shell (8) and a sample adding mechanism, the shell (8) is fixed above the PVC plate (1), the sample pad (2), the colloidal gold combined pad (3), the nitrocellulose membrane (4) and the water absorption pad (5) are all positioned at the inner side of the shell (8), the sample adding mechanism is positioned above the sample pad (2), the shell (8) is provided with an observation part (10), the shell (8) is made of transparent plastic, and the observation part (10) is positioned above the nitrocellulose membrane (4);

the sample adding mechanism comprises a fixed tube (11), a sample adding tube (12), a sample adding component, a sample adding port, a sealing component and a fixed component, wherein the sample adding port is arranged on a shell (8), the fixed tube (11) is fixed at the top in the shell (8), the periphery of the sample adding tube (12) is connected with the inner wall of the sample adding port in a sealing manner, the sample adding component is positioned at the inner side of the fixed tube (11), the sample adding component comprises a transverse rod (13), a vertical rod (14), a sealing plug (15) and a sealing ring (16), the periphery of the sealing ring (16) is fixed on the inner wall of the fixed tube (11), the two ends of the transverse rod (13) are fixed on the inner wall of the sample adding tube (12), the sealing plug (15) is fixed below the transverse rod (13) through the vertical rod (14), the vertical rod (14) is positioned at the inner side of the sample adding port, the two sides of the upper part of the sample adding tube (12), curb plate (17) and application of sample pipe (12) fixed connection, the both ends of spring (18) respectively with diaphragm and casing (8) fixed connection, spring (18) are in compression state, seal assembly is connected with one of them curb plate (17), seal assembly includes apron (19) and rotation unit, the top at application of sample pipe (12) is established to apron (19) lid, apron (19) are connected with one of them curb plate (17) through rotating the unit, fixed subassembly sets up the one end of rotating the unit of keeping away from at apron (19).

2. The furaltadone metabolite rapid detection card according to claim 1, wherein the observation portion (10) is an upwardly convex structure.

3. The furaltadone metabolite rapid detection card according to claim 1, wherein an end of the sample application tube (12) away from the fixed tube (11) is shaped like a horn.

4. The furaltadone metabolite rapid detection card of claim 1, wherein the rotation unit comprises a collar (20), a convex plate (21) and a rotation rod (22), the collar (20) is fixed on the side rod, the convex plate (21) is fixed under the cover plate (19) through the rotation rod (22), and the collar (20) is sleeved on the rotation rod (22).

5. The furaltadone metabolite rapid detection card according to claim 1, wherein the fixing component comprises a fixing block (23), a rotating shaft (24) and two clamping plates (9), the vertical section of the fixing block (23) is U-shaped, one end of the cover plate (19) far away from the rotating unit is positioned in the U-shaped opening of the fixing block (23), the fixing block (23) is positioned at the top end of the rotating shaft (24), the two clamping plates (9) are respectively positioned above the housing (8) and at the top inside the housing (8), and the clamping plates (9) are fixed on the rotating shaft (24).

6. Furaltadone metabolite rapid detection card according to claim 1, characterized in that the sealing plug (15) is shaped as a conical cylinder, the outer diameter of the top of the sealing plug (15) is smaller than the inner diameter of the sealing ring (16), and the outer diameter of the bottom of the sealing plug (15) is larger than the inner diameter of the sealing ring (16).