CN211463195U - Centrifugal micro-fluidic chip system - Google Patents
Centrifugal micro-fluidic chip system Download PDFInfo
- Publication number
- CN211463195U CN211463195U CN201921597640.XU CN201921597640U CN211463195U CN 211463195 U CN211463195 U CN 211463195U CN 201921597640 U CN201921597640 U CN 201921597640U CN 211463195 U CN211463195 U CN 211463195U
- Authority
- CN
- China
- Prior art keywords
- chip
- centrifugal
- sub
- sample
- micro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The utility model belongs to the technical field of micro-fluidic chip, a centrifugal micro-fluidic chip system is disclosed, include: a chip body provided with a micro flow channel structure; the centrifugal clamp is used for fixing the chip body; the centrifugal driving mechanism is used for driving the centrifugal clamp to rotate; the chip body comprises a plurality of sub-chips, each sub-chip is provided with an independent micro-channel structure, and the sub-chips are detachably mounted on the centrifugal clamp. The utility model provides a pair of centrifugal micro-fluidic chip system cuts apart into a plurality of sub-chip structures with traditional integral centrifugal micro-fluidic chip, is about to a sub-chip that single index development becomes the chip body, according to the individual condition, the index of testing as required makes up, realizes quick, convenient, low-cost individualized diagnosis needs, has solved the problem of current integral centrifugal micro-fluidic chip flexibility.
Description
Technical Field
The utility model relates to a micro-fluidic chip technical field especially relates to a centrifugal micro-fluidic chip system.
Background
The current diagnostic techniques usually require multiple indicators to determine whether a disease is present in a patient, and the indicators are different according to individual conditions. For example, the detection of tumor markers has great difference between detection indexes of men and women. Even if the sex is the same, the difference of age, living habits and medical history needs to be detected by matching with different indexes.
The centrifugal microfluidic chip is gradually applied to the field of diagnosis due to the characteristics of simple operation and high flux.
If the traditional centrifugal microfluidic chip is used as a detection carrier, the flexibility is poor, a new product needs to be developed for each combination, for example, AFP + CEA and AFP + CEA + CA125 are two different products, so that the problems of poor product flexibility and high product development, transportation and management costs are caused.
Based on the above situation, there is a need to design a centrifugal microfluidic chip system capable of solving the above problems.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a centrifugal micro-fluidic chip system to it is not enough to solve current integral centrifugal micro-fluidic chip flexibility, and single centrifugal micro-fluidic chip can only detect fixed index, can not satisfy the technical problem of individualized diagnosis demand.
To achieve the purpose, the utility model adopts the following technical proposal:
a centrifugal microfluidic chip system, comprising:
a chip body provided with a micro flow channel structure;
the centrifugal clamp is used for fixing the chip body;
the centrifugal driving mechanism is used for driving the centrifugal clamp to rotate;
the chip body comprises a plurality of sub-chips, each sub-chip is provided with an independent micro-channel structure, and the sub-chips are detachably mounted on the centrifugal clamp.
Furthermore, the centrifugal microfluidic chip system further comprises a sample distribution mechanism, the sample distribution mechanism is provided with a distribution pipeline communicated with the micro-channel structure of the sub-chip, and the sample distribution mechanism is fixedly connected with the chip body.
Further, the sample distribution mechanism and the centrifugal clamp are of an integrally formed structure.
Further, the sample distribution mechanism and the centrifugal clamp are of a split connection structure.
Furthermore, a whole blood filtering membrane is arranged between the distribution pipeline and the sub-chip micro-channel structure.
Furthermore, the micro-channel structure of the sub-chip comprises a reagent injection port, a sample injection port and a reaction cavity.
Furthermore, an identification mark is arranged on the sub-chip.
Further, the shape of the sub-chip is fan-shaped, triangular or trapezoidal.
Further, when the number of the sub-chips is an odd number, the chip body further comprises a leveling block.
Furthermore, a plurality of fixing grooves matched with the sub-chips in shape are formed in the centrifugal clamp.
The utility model has the advantages that: the centrifugal microfluidic chip system is provided, a traditional integral centrifugal microfluidic chip is divided into a plurality of sub-chip structures, namely, a single index is developed into one sub-chip of a chip body, and the sub-chip is combined according to individual conditions and indexes to be tested, so that the personalized diagnosis requirement of rapidness, convenience and low cost is realized, and the problem of flexibility of the existing integral centrifugal microfluidic chip is solved; be provided with sample distribution mechanism, when a plurality of indicators of single sample detection of needs, only need carry out the application of sample once, can distribute to in every sub-chip.
Drawings
Fig. 1 is a schematic structural view of a centrifugal microfluidic system according to an embodiment of the present invention;
fig. 2 is a schematic view of a neutron chip and a centrifugal jig before assembly according to an embodiment of the present invention;
fig. 3 is a schematic structural view of a centrifugal jig according to a first embodiment of the present invention;
fig. 4 is a schematic structural diagram of a neutron chip according to an embodiment of the present invention;
fig. 5 is a schematic view of a centrifugal microfluidic system according to a second embodiment of the present invention before assembly;
fig. 6 is a schematic view of a centrifugal microfluidic system according to a second embodiment of the present invention after assembly;
fig. 7 is a schematic structural view of a centrifugal jig according to a second embodiment of the present invention;
fig. 8 is a schematic view of a centrifugal microfluidic system according to a third embodiment of the present invention before assembly;
fig. 9 is a schematic view of a centrifugal microfluidic system according to a third embodiment of the present invention after assembly.
The chip comprises a chip body 1, a centrifugal clamp 2, a sub-chip 3, a sample distribution mechanism 4, a whole blood filtering membrane 5, a fixing groove 21, a reagent injection port 31, a sample injection port 32, a reaction cavity 33, an identification mark 34, a sample adding pool 41 and a distribution pipeline 42.
Detailed Description
In order to further understand and appreciate the structural features and advantages of the present invention, preferred embodiments and the accompanying drawings are described in detail as follows:
example one
As shown in fig. 1 to 4, a centrifugal microfluidic chip system includes:
the chip body 1 is provided with a micro-channel structure;
the centrifugal clamp 2 is used for fixing the chip body 1;
a centrifugal driving mechanism (not shown in the figure) for driving the centrifugal jig 2 to rotate;
the chip body 1 comprises a plurality of sub-chips 3, each sub-chip 3 is provided with an independent micro-channel structure, and the sub-chips 3 are detachably mounted on the centrifugal clamp 2.
In this centrifugal micro-fluidic chip system, chip body 1 is designed into the independent sub-chip 3 of polylith, constitutes complete chip body 1 when polylith sub-chip 3 assembles on centrifugal jig 2 promptly, and sub-chip 3 can set up the microchannel structure according to the user demand, and the microchannel structure on a plurality of sub-chips 3 can be the same also can not be the same. The centrifugal driving mechanism (not shown in the figure) drives the centrifugal clamp 2 to rotate around the central axis of the centrifugal clamp 2, and the chip body 1 on the centrifugal clamp 2 also rotates around the central axis of the centrifugal clamp, namely, performs centrifugal motion. The centrifugal driving mechanism may be a centrifuge commonly used in the art, and is not limited herein. To facilitate the processes of sample application, etc., the sub-chip 3 is mounted on the upper side of the centrifugal jig 2, and the sub-chip 3 can be fixed on the centrifugal jig 2 by using conventional fixing means such as adhesive, bolts, clamps, etc. The chip body 1 can be divided into 2 parts or more, that is, the number of the sub-chips is 2 or more, and is usually 24 parts or less, preferably 4 to 12 parts, according to the actual situation, and a plurality of the sub-chips 3 constitute one centrifugal chip.
The processing method of the sub-chip 3 comprises different modes such as hot pressing, plasma, gluing, laser, ultrasonic sealing and the like. The material of the sub-chip 3 includes glass, silicon wafer, or common polymer material. The polymer material includes Polydimethylsiloxane (PDMS), polyurethane, epoxy resin, polymethyl methacrylate (PMMA), Polycarbonate (PC), Cyclic Olefin Copolymer (COC), Polystyrene (PS), Polyethylene (PE) and fluoroplastic. The material may be one or more of the above materials.
In this embodiment, the micro flow channel structure of the sub-chip 3 includes a reagent injection port 31, a sample injection port 32, and a reaction chamber 33. Common molecular, immune, biochemical diagnosis and analysis can be independently accomplished in each sub-chip 3, the utility model discloses do not restrict centrifugal micro-fluidic chip system's specific application. The centrifugal microfluidic chip system has the characteristic of typical high flux, is preferably used in the field of single-sample multi-index (the same patient sample can detect a plurality of different tumor markers), and can also be applied in the field of single-sample single-index (a plurality of patient samples can detect the same tumor marker respectively) according to requirements. If the sub-chips 3 are applied to the field of single sample and single index, different samples to be detected are added into the sample injection port of each sub-chip 3.
In this embodiment, the sub-chip 3 is provided with an identification mark 34. The identification mark 34 is a one-dimensional code, a two-dimensional code, and other conventional identification means, and the identification mark 34 on each sub-chip 3 is different according to the detection item, for example, the tumor markers AFP and CEA may adopt the one-dimensional code, the two-dimensional code, and other conventional identification means, and the identification mark 34 of the sub-chip 3 is detected by an identification module (not shown in the figure) to distinguish the specific detection item, and the identification mark 34 contains information of sample addition, reaction, detection, and the like of the independent sub-chip 3, and can be matched through equipment. The identification module is an identification device corresponding to the type of the identification mark 34, such as a barcode reading device and a two-dimensional code reader, and the structure and principle thereof are all prior art and are not described herein again.
In the present embodiment, the shape of the sub-chip 3 is a sector. In other embodiments, the shape of the sub-chips may also be triangular or trapezoidal. The plurality of sub-chips 3 are assembled into the chip body 1.
In the present embodiment, when the number of the sub-chips 3 is odd, the chip body 1 further includes a leveling block (not shown). The angles occupied by the sub-chips 3 of each fan-shaped structure can be the same or different, the angles are determined according to the number of the sub-chips 3, the angles of each sub-chip 3 do not need to be completely the same, and the angles of the sub-chips 3 at the optimal diagonal positions are consistent, so that the balancing is ensured, and the centrifugal motion of the whole structure is ensured not to generate severe deflection. If the sizes of the sub-chips 3 with the diagonal fan-shaped structures are consistent, but due to the fact that odd number of detection items may appear in practical application, the sub-chips 3 with the fan-shaped structures can appear, a leveling block needs to be arranged, the leveling block is a blank structure (such as a fan-shaped structure without a micro-channel structure) which is the same as or similar to the appearance of the sub-chips 3, the leveling block is arranged, basic leveling of the chip body 1 consisting of the sub-chips 3 with the fan-shaped structures at each angle is ensured, and severe shaking in the centrifugal process is avoided.
In this embodiment, the centrifugal jig 2 is provided with a plurality of fixing grooves 21 having a shape matching with the shape of the sub-chip 3. The lower part of the sub-chip 3 may be provided with a protrusion (not shown) matching with the shape of the fixing groove, so as to facilitate the installation and fixation of the sub-chip 3 and the centrifugal clamp 2.
Example two
The difference between the present embodiment and the first embodiment is:
as shown in fig. 5 to 7, in the present embodiment, the centrifugal microfluidic chip system further includes a sample distributing mechanism 4, the sample distributing mechanism 4 is provided with a distributing channel communicated with the micro channel structure of the sub-chip 3, and the sample distributing mechanism 4 is fixedly connected to the chip body 1.
In the clinical examination process, a single sample and multiple indexes (the same patient sample is used for detecting multiple different tumor markers) are mostly needed, namely, the single sample is used for detecting multiple indexes. To apply this embodiment to the above application targets, a sample distribution structure 4 is provided to avoid multiple sample additions. The sample distribution structure 4 comprises a sample reservoir 41 and a plurality of distribution channels 42, wherein the inlet ends of the distribution channels 42 are communicated with the sample reservoir 41, and the outlet ends of the distribution channels 42 are communicated with the micro-channel structure of the sub-chip 3, i.e. communicated with the sample injection port 32 of the sub-chip 3. The distribution pipeline 42 is arranged along the circumferential direction of the sample adding pool 41, when a sample to be detected is added into the sample adding pool 41 through a liquid transfer device (not shown in the figure), because the sample distribution mechanism 4 is fixedly connected with the chip body 1, the sub-chip 3 and the sample distribution mechanism 4 are driven by the centrifugal driving mechanism to rotate, in the centrifugal rotating process, the sample to be detected is evenly distributed into each distribution pipeline 42, and finally, the outlet end of the sample distribution mechanism 4 is communicated with the sub-chip 3, so that the sample introduction purpose is achieved. The sample dispensing mechanism 4 can serve as a reagent injection structure, and common reagents required for the respective sub-chips 3 can be dispensed through the sample dispensing mechanism 4. The outlet end of the sample distribution mechanism 4 corresponds to the sample injection port 32, wherein the outlet end is provided with a convex structure, and is inserted into the sample injection port 32 and is in sealing assembly.
In the present embodiment, the sample dispensing mechanism 4 and the centrifugal clamp 2 are integrally formed. The sample adding pool 41 of the sample distributing mechanism 4 is formed and arranged at the central part of the centrifugal clamp 2, the distributing pipeline 42 is arranged in the centrifugal clamp 2 along the circumferential direction of the sample adding pool 41, and when the sub-chip 3 is installed on the centrifugal clamp 2, the outlet end of the distributing pipeline 42 is communicated with the sample injection port 32 of the sub-chip 3. Since the outlet end of the distribution pipe 42 is disposed upward at this time, the sample injection port 32 of the sub-chip 3 is disposed downward.
In this embodiment, a whole blood filtration membrane 5 is provided between the distribution channel 42 and the micro flow channel structure of the sub-chip 3. In the practical application process, the sample added into the sample adding pool 41 may be whole blood, and most of the detection requirements separate the whole blood into serum, so that a whole blood filtering membrane 5 needs to be added between the outlet end of the distribution pipeline 42 and the sample injection port 32, the whole blood filtering membrane 5 is a commercially available product, the whole blood filtering membrane 5 has certain flexibility, and the risk of solution leakage between the outlet end of the distribution pipeline 42 and the sample injection port 32 is further avoided.
Other features of the centrifugal microfluidic chip system in this embodiment are the same as those of the first embodiment, and are not described herein again.
EXAMPLE III
The present embodiment is different from the second embodiment in that:
as shown in fig. 8 to 9, in the present embodiment, the sample dispensing mechanism 4 and the centrifugal clamp 2 are a split connection structure. The sample adding pool 41 of the sample distributing mechanism 4 is arranged at the central part of the sample distributing mechanism 4, the distributing pipeline 42 is arranged in the sample distributing mechanism 4 along the circumferential direction of the sample adding pool 41, and when the sample distributing mechanism 4 is arranged on the chip body 1 after the sub-chip 3 is arranged on the centrifugal clamp 2, the outlet end of the distributing pipeline 42 is communicated with the sample injection port 32 of the sub-chip 3. Since the outlet end of the distribution pipe 42 is disposed downward at this time, the sample injection port 32 of the sub-chip 3 is disposed upward.
Other features of the centrifugal microfluidic chip system in this embodiment are the same as those of the second embodiment, and are not described herein again.
Example four
Take double-antibody sandwich chemiluminescence as an example, and detail the application of the utility model in the detection process of a plurality of tumor markers in a single sample.
The detection of the tumor marker is matched with different marker combinations according to individual difference requirements, and the tumor marker is generally detected clinically at present by adopting a chemiluminescence method.
In the application process, medical staff can select different tumor markers to be matched according to the condition of a patient, the detection of each tumor marker is integrated on the sub-chip 3 with the sector structure, and the embodiment takes 6 marker detections (CEA, CYFRA21-1, NSE, SCC, CA-159 and AFP) as an example.
The detection index corresponding to each sub-chip 3 has a definite identification mark 34 on the sub-chip 3, and can be directly interpreted by identification equipment or naked eyes.
The primary inspection mechanism needs whole blood detection, and firstly, a sample distribution mechanism 4, a whole blood filtering membrane 5 and a corresponding sub-chip 3 are sequentially assembled on a centrifugal clamp 2.
Add a certain amount of whole blood into application of sample pond 41 through the application of sample device, under the centrifugal force drives, whole blood evenly distributes to each distribution pipeline 42, and whole blood realizes the whole blood filtration through pressing from both sides whole blood filtration membrane 5 at sample distribution mechanism 4 and sub-chip 3, and serum gets into sub-chip 3's sample introduction port 32 under the drive of centrifugal force.
Other reagents required by chemiluminescence detection are added through the reagent injection port 31, reaction and detection are carried out in the reaction cavity 33, and the identification mark 34 of each sub-chip 3 can be interpreted through identification equipment, so that information such as the reaction process, time and the like of the independent sub-chip 3 can be determined and adjusted.
For chemiluminescence detection, the sub-chips 3 of the fan-shaped structures all need common reagents such as cleaning solution, diluent and the like, the sample distribution mechanism 4 can serve as a reagent sample introduction structure, and the common reagents can be distributed through the sample distribution mechanism 4.
The parts not related to in the utility model are all the same with the prior art or can be realized by adopting the prior art.
Finally, it should be noted that: in the description of the present invention, the terms "center", "upper", "lower", "left", "right", "front", "rear", "inner", "outer", "vertical", "horizontal", etc. indicate that the directions or positional relationships are based on the directions or positional relationships shown in the drawings, and are only for convenience of description and understanding of the technical solutions of the present invention, but do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art. The above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.
Claims (10)
1. A centrifugal microfluidic chip system, comprising:
a chip body provided with a micro flow channel structure;
the centrifugal clamp is used for fixing the chip body;
the centrifugal driving mechanism is used for driving the centrifugal clamp to rotate;
the chip body comprises a plurality of sub-chips, each sub-chip is provided with an independent micro-channel structure, and the sub-chips are detachably mounted on the centrifugal clamp.
2. The centrifugal microfluidic chip system according to claim 1, further comprising a sample distribution mechanism, wherein the sample distribution mechanism is provided with a distribution channel communicated with the micro channel structure of the sub-chip, and the sample distribution mechanism is fixedly connected to the chip body.
3. A centrifugal microfluidic chip system according to claim 2, wherein the sample dispensing mechanism and the centrifugal clamp are integrally formed.
4. The microfluidic chip system according to claim 2, wherein the sample dispensing mechanism and the centrifugal clamp are a split structure.
5. A centrifugal microfluidic chip system according to claim 2, wherein a whole blood filtering membrane is disposed between said distribution channel and said sub-chip microchannel structure.
6. The system of claim 1, wherein the micro flow channel structure of the sub-chip comprises a reagent inlet, a sample inlet, and a reaction chamber.
7. A centrifugal microfluidic chip system according to claim 1, wherein said sub-chip is provided with an identification mark.
8. A centrifugal microfluidic chip system according to claim 1, wherein said sub-chips are fan-shaped, triangular or trapezoidal in shape.
9. A centrifugal microfluidic chip system according to claim 1, wherein when the number of said sub-chips is odd, said chip body further comprises a leveling block.
10. The centrifugal microfluidic chip system according to claim 1, wherein the centrifugal jig has a plurality of fixing grooves with a shape matching with the shape of the sub-chip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921597640.XU CN211463195U (en) | 2019-09-24 | 2019-09-24 | Centrifugal micro-fluidic chip system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921597640.XU CN211463195U (en) | 2019-09-24 | 2019-09-24 | Centrifugal micro-fluidic chip system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211463195U true CN211463195U (en) | 2020-09-11 |
Family
ID=72357238
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921597640.XU Active CN211463195U (en) | 2019-09-24 | 2019-09-24 | Centrifugal micro-fluidic chip system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211463195U (en) |
-
2019
- 2019-09-24 CN CN201921597640.XU patent/CN211463195U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103831140B (en) | A kind of micro-fluidic chip of multiple determination | |
| US10092902B2 (en) | Fluid interface cartridge for a microfluidic chip | |
| EP2870391B1 (en) | Assay cartridge valve system | |
| US7223363B2 (en) | Method and system for microfluidic interfacing to arrays | |
| ES2842969T3 (en) | Thin film layer centrifuge device and analysis method using the same | |
| US7718420B2 (en) | Microfluidic biochip for blood typing based on agglutination reaction | |
| JP5337912B2 (en) | Sheath flow apparatus and method | |
| US10634602B2 (en) | Fluidic cartridge for cytometry and additional analysis | |
| KR20150097764A (en) | Portable fluorescence detection system and microassay cartridge | |
| US20140186963A1 (en) | Microfluid Testing System with a Multiple-Channel Disc and Utility thereof | |
| US11566224B2 (en) | Dendritic cell generator | |
| US11781959B2 (en) | Methods and systems for sample extraction | |
| EP2613882B1 (en) | Microfluidic capsule | |
| US20080274451A1 (en) | Body for flow-through cells and the use thereof | |
| CN209923319U (en) | Microfluidic chip structure and analysis device | |
| Sabourin et al. | The main STREAM component platform: a holistic approach to microfluidic system design | |
| KR20140008976A (en) | Microfluidic structure, microfluidic device having the same and method controlling the microfluidic device | |
| CN210085460U (en) | Microfluidic chip structure and analysis device | |
| CN211463195U (en) | Centrifugal micro-fluidic chip system | |
| CN105628660B (en) | A kind of passive micro-valve POCT chips | |
| Azhar et al. | Microfluidic platforms for point of care (POC) medical diagnostics | |
| CN110938523A (en) | Centrifugal microfluidic chip, system and detection method for SAT | |
| CN103852518A (en) | Multi-channel independent control integrated micro-fluidic chip based on thin film acoustic wave device | |
| KR20220046751A (en) | System for single or multi conducting Biochemistry, Immunoassay, Blood test and Gene analysis, and Multi-Disc therefor | |
| CN203750554U (en) | Micro-fluidic chip capable of being detected by multiple indexes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A centrifugal microfluidic chip system Effective date of registration: 20220728 Granted publication date: 20200911 Pledgee: China Development Bank Shenzhen Branch Pledgor: Dongguan Dongyang Sunshine Medical Intelligent Device R&D Co.,Ltd. Registration number: Y2022980011450 |