CN211420032U

CN211420032U - A draw purification device for preparing antibody

Info

Publication number: CN211420032U
Application number: CN201922055080.1U
Authority: CN
Inventors: 陈�胜
Original assignee: China Kean Ti Tangshan Biotechnology Co ltd
Current assignee: China Kean Ti Tangshan Biotechnology Co ltd
Priority date: 2019-11-25
Filing date: 2019-11-25
Publication date: 2020-09-04
Anticipated expiration: 2029-11-25

Abstract

The utility model relates to an antibody preparation technical field especially relates to an extraction and purification device for preparing antibody, when using the extraction and purification device for preparing antibody to extract antibody stoste, carries out quantitative addition to the stoste through first measuring pump, carries out quantitative addition to the medicament through back measuring pump and preceding measuring pump simultaneously to guarantee the accuracy of medicament addition, effectively improve subsequent extraction effect; the device comprises a base, a mixing cylinder, a liquid outlet pipe, a precipitation condensing box and a liquid discharge pipe, wherein an inner cavity is formed in the mixing cylinder; the device also comprises a liquid storage tank, a connecting pipe, a first metering pump, a liquid inlet pipe, a bottom plate, a base, a motor, a reduction gearbox, a rotating shaft, a rotating blade, a rear support, a rear solenoid, a rear screw rod, a rear chemical box, a rear metering pump, a front support, a front solenoid, a front screw rod, a front chemical box, a front chemical tube and a front metering pump.

Description

A draw purification device for preparing antibody

Technical Field

The utility model relates to an antibody preparation technical field especially relates to an extract purification device for preparing antibody.

Background

As is well known, an extraction and purification device for preparing antibodies is an auxiliary device used in the antibody production and processing process for screening and purifying an antibody stock solution to better extract and purify the antibody stock solution, and is widely used in the technical field of antibody preparation; the existing extraction and purification device for preparing the antibody needs to add a medicament into an antibody stock solution, filter and precipitate, but most of the existing medicament adding methods are directly and manually measured and added, so that the accuracy of the medicament adding amount is low, and the extraction effect is influenced.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model provides a can carry out the accuracy to the medicament and add, effectively improve and draw the extraction and purification device that is used for preparing the antibody of effect.

The utility model discloses an extraction and purification device for preparing antibody, including base, mixing drum, drain pipe, precipitation condensing box and fluid-discharge tube, the mixing drum is fixed to be set up in the top of base, the inside of mixing drum is provided with the inner chamber, the drain pipe is fixed to be set up in the partial downside of mixing drum and communicates with each other with the inner chamber, precipitation condensing box is located the right-hand side of mixing drum, the right-hand member of drain pipe is fixed with the feed liquor end of precipitation condensing box and is connected, the fluid-discharge tube is fixed to be set up in the bottom of precipitation condensing box; the automatic mixing device also comprises a liquid storage tank, a connecting pipe, a first metering pump, a liquid inlet pipe, a bottom plate, a machine base, a motor, a reduction gearbox, a rotating shaft, a rotating blade, a rear support, a rear screw rod, a rear chemical box, a rear metering pump, a front support, a front screw, a front chemical box, a front chemical pipe and a front metering pump, wherein the liquid storage tank is fixedly arranged on the left side of the top end of the mixing cylinder, the connecting pipe is fixedly arranged at the liquid outlet of the liquid storage tank, the first metering pump is fixedly arranged on the right side of the top end of the mixing cylinder, the right end of the connecting pipe is fixedly connected with the left end of the first metering pump, the rear end of the liquid inlet pipe is fixedly connected with the front end of the first metering pump, the bottom of the liquid inlet pipe is inserted into the inner cavity from, the rear part of the motor is fixedly connected with the front part of the reduction gearbox, the rotating shaft is fixedly arranged at the top end of the reduction gearbox, the top end of the rotating shaft penetrates through the base from the top end of the base and is inserted into the inner cavity, the rotating blade is fixedly arranged in the middle of the rotating shaft, the rear support is fixedly arranged on the left rear side of the top end of the base, the rear screw tube is rotatably arranged at the top end of the rear support, the bottom end of the rear screw rod is inserted into the top end of the rear screw tube from the top end of the rear screw tube and is screwed in the inner cavity, the top end of the rear screw rod is fixedly connected with the bottom end of the rear medicament box, the rear medicament inlet tube is fixedly arranged at the bottom end of the rear medicament box, the right part of the rear medicament inlet tube is inserted into the inner cavity from the rear end of the mixing cylinder, the rear metering pump is fixedly arranged in the middle of the rear medicament tube, the front support, the top of preceding screw rod and the bottom fixed connection of preceding explosive box, preceding explosive tube is fixed to be set up in the bottom of preceding explosive box, and the right part of the explosive tube that advances inserts the inner chamber from the front end of mixing drum, and preceding measuring pump is fixed to be set up in the middle part of advancing the explosive tube.

The utility model discloses an extract purification device for preparing antibody still includes shock attenuation post, shock attenuation cover and damping spring, and the shock attenuation post is fixed to be set up in the bottom of base, and the shock attenuation cover is inside to be provided with the shock attenuation chamber, and damping spring is fixed to be set up in the bottom of shock attenuation chamber, and the inner chamber is inserted from the top of shock attenuation cover in the bottom of shock attenuation post.

The utility model discloses an extract purification device for preparing antibody still includes the filter screen, and the filter screen is fixed to be set up in the bottom of inner chamber.

The utility model discloses an extract purification device for preparing antibody still includes the limiting plate, and the limiting plate is fixed to be set up in the bottom of shock attenuation post, and the bottom of limiting plate and damping spring's top contact, the bottom of limiting plate are provided with spacing pipe, and the bottom of shock attenuation cover is provided with the gag lever post and is located damping spring's inner, and inside the bottom of spacing pipe was inserted from the bottom of spacing pipe in the top of gag lever post.

The utility model discloses an extract purification device for preparing antibody still includes the scraper blade and arranges miscellaneous pipe, and the bottom of pivot is provided with the horizontal pole, and the scraper blade is fixed to be set up in the bottom of horizontal pole, the bottom of scraper blade and the bottom contact of inner chamber, and the bottom right side of inner chamber is provided with arranges miscellaneous mouthful, arranges the top of miscellaneous pipe and arranges the bottom fixed connection of miscellaneous mouthful.

The utility model discloses an extract purification device for preparing antibody still includes row's miscellaneous valve, arranges miscellaneous valve fixed the setting in the middle part of arranging miscellaneous pipe.

The utility model discloses an extract purification device for preparing antibody still includes pillar and footing, and the pillar is fixed to be set up in the bottom of bottom plate, and the footing is fixed to be set up in the bottom of pillar.

The utility model discloses an extract purification device for preparing antibody still includes the fluid infusion pipe, and the fluid infusion pipe is fixed to be set up in the top of liquid reserve tank.

Compared with the prior art, the beneficial effects of the utility model are that: when using the extraction purification device who is used for preparing the antibody to extract the antibody stoste, carry out the ration through first measuring pump and add to the stoste, carry out the ration through back measuring pump and preceding measuring pump and add to the medicament simultaneously to guarantee the accuracy of medicament addition, effectively improve subsequent extraction effect.

Drawings

Fig. 1 is a schematic perspective view of the structure of the present invention;

FIG. 2 is a front view of the structure of the present invention;

fig. 3 is a schematic top view of the structure of the present invention;

FIG. 4 is a left side view of the structure of the present invention;

FIG. 5 is a right-side view schematically illustrating the structure of the present invention;

fig. 6 is a front sectional view of the present invention;

FIG. 7 is a schematic view of the internal structure of the damping sleeve of the present invention;

in the drawings, the reference numbers: 1. a base; 2. a mixing drum; 3. a liquid outlet pipe; 4. a precipitation condensing tank; 5. a liquid discharge pipe; 6. a liquid storage tank; 7. a connecting pipe; 8. a first metering pump; 9. a liquid inlet pipe; 10. a base plate; 11. a machine base; 12. a motor; 13. a reduction gearbox; 14. a rotating shaft; 15. a rotating blade; 16. a rear bracket; 17. a rear solenoid; 18. a rear screw; 19. a rear medicament box; 20. then feeding the powder into a powder tube; 21. a rear metering pump; 22. a front bracket; 23. a front solenoid; 24. a front screw; 25. a front medicament box; 26. advancing the medicine tube; 27. a front metering pump; 28. a shock-absorbing post; 29. a shock-absorbing sleeve; 30. a damping spring; 31. a filter screen; 32. a limiting plate; 33. a squeegee; 34. a trash removal pipe; 35. a trash valve; 36. a pillar; 37. footing; 38. and a liquid replenishing pipe.

Detailed Description

The following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings and examples. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.

As shown in fig. 1 to 7, the utility model discloses an extraction and purification device for preparing antibody, including base 1, mixing drum 2, drain pipe 3, precipitation condensing box 4 and fluid-discharge tube 5, mixing drum 2 is fixed to be set up in the top of base 1, the inside of mixing drum 2 is provided with the inner chamber, drain pipe 3 is fixed to be set up in the partial downside of mixing drum 2 and communicates with each other with the inner chamber, precipitation condensing box 4 is located the right-hand side of mixing drum 2, the right-hand member of drain pipe 3 and the inlet end fixed connection of precipitation condensing box 4, fluid-discharge tube 5 is fixed to be set up in the bottom of precipitation condensing box 4; the automatic mixing device further comprises a liquid storage tank 6, a connecting pipe 7, a first metering pump 8, a liquid inlet pipe 9, a bottom plate 10, a machine base 11, a motor 12, a reduction gearbox 13, a rotating shaft 14, a rotating blade 15, a rear support 16, a rear screw 17, a rear screw 18, a rear chemical box 19, a rear chemical pipe 20, a rear metering pump 21, a front support 22, a front screw 23, a front screw 24, a front chemical box 25, a front chemical pipe 26 and a front metering pump 27, wherein the liquid storage tank 6 is fixedly arranged on the left side of the top end of the mixing cylinder 2, the connecting pipe 7 is fixedly arranged on the liquid outlet of the liquid storage tank 6, the first metering pump 8 is fixedly arranged on the right side of the top end of the mixing cylinder 2, the right end of the connecting pipe 7 is fixedly connected with the left end of the first metering pump 8, the rear portion of the liquid inlet pipe 9 is fixedly connected with the front end of the first metering pump 8, the bottom of the liquid inlet pipe 9 is inserted, the motor 12 is fixedly arranged at the top end of the base 11, the reduction box 13 is fixedly arranged at the rear side of the top end of the bottom plate 10, the rear part of the motor 12 is fixedly connected with the front part of the reduction box 13, the rotating shaft 14 is fixedly arranged at the top end of the reduction box 13, the top end of the rotating shaft 14 penetrates through the base 1 from the top end of the base 1 and is inserted into the inner cavity, the rotating blade 15 is fixedly arranged at the middle part of the rotating shaft 14, the rear support 16 is fixedly arranged at the left rear side of the top end of the base 1, the rear screw tube 17 is rotatably arranged at the top end of the rear support 16, the bottom end of the rear screw 18 is inserted into the inner cavity from the top end of the rear screw tube 17 and is screwed in the top end of the rear screw tube 17, the top end of the rear screw 18 is fixedly connected with the bottom end of the rear chemical box 19, the rear chemical tube 20 is fixedly arranged, the front support 22 is fixedly arranged on the left front side of the top end of the base 1, the front screw 23 is rotatably arranged on the top end of the front support 22, the bottom end of the front screw 24 is inserted into the top end of the front screw 23 and is screwed into the top end of the front screw 23, the top end of the front screw 24 is fixedly connected with the bottom end of the front medicament box 25, the front medicament tube 26 is fixedly arranged on the bottom end of the front medicament box 25, the right part of the front medicament tube 26 is inserted into the inner cavity from the front end of the mixing barrel 2, and the front metering pump 27 is fixedly arranged in the middle of the front medicament tube 26; when using the extraction purification device who is used for preparing the antibody to extract the antibody stoste, carry out the ration through first measuring pump and add to the stoste, carry out the ration through back measuring pump and preceding measuring pump and add to the medicament simultaneously to guarantee the accuracy of medicament addition, effectively improve subsequent extraction effect.

The utility model discloses an extraction and purification device for preparing antibody, still include shock-absorbing column 28, shock-absorbing sleeve 29 and damping spring 30, shock-absorbing column 28 is fixed to be set up in the bottom of base 1, and shock-absorbing chamber is provided with in shock-absorbing sleeve 29, and damping spring 30 is fixed to be set up in the bottom of shock-absorbing chamber, and the bottom of shock-absorbing column 28 inserts the inner chamber from the top of shock-absorbing sleeve 29; the damping performance of the device is improved through the damping spring.

The utility model discloses an extraction and purification device for preparing antibody, which also comprises a filter screen 31, wherein the filter screen 31 is fixedly arranged at the bottom end of the inner cavity; and (4) preliminarily filtering the antibody stock solution through a filter screen.

The utility model discloses an extract purification device for preparing antibody still includes limiting plate 32, and limiting plate 32 is fixed to be set up in the bottom of shock absorber column 28, and the bottom of limiting plate 32 contacts with the top of damping spring 30, and the bottom of limiting plate 32 is provided with spacing pipe, and the bottom of shock attenuation cover 29 is provided with the gag lever post and is located the inner of damping spring 30, and the top of gag lever post inserts the bottom inside of spacing pipe from the bottom of spacing pipe; the limiting plate prevents the damping column from dropping off the damping sleeve.

The utility model discloses an extraction and purification device for preparing antibody, still include scraper blade 33 and arrange miscellaneous pipe 34, the bottom of pivot 14 is provided with the horizontal pole, scraper blade 33 is fixed to be set up in the bottom of horizontal pole, the bottom of scraper blade 33 contacts with the bottom of inner chamber, the bottom right side of inner chamber is provided with arranges miscellaneous mouthful, arrange the top of miscellaneous pipe 34 and arrange the bottom fixed connection of miscellaneous mouthful; the sediment is scraped to the impurity discharge opening through a scraper blade and is discharged.

The utility model discloses an extraction and purification device for preparing antibody, which also comprises an impurity discharging valve 35, wherein the impurity discharging valve 35 is fixedly arranged in the middle of the impurity discharging pipe 34; and controlling impurity removal through an impurity removal valve.

The utility model discloses an extraction and purification device for preparing antibody, still include pillar 36 and footing 37, pillar 36 is fixed to be set up in the bottom of bottom plate 10, footing 37 is fixed to be set up in the bottom of pillar 36; the support device is stabilized by the feet.

The utility model discloses an extraction and purification device for preparing antibody, which also comprises a liquid supplementing pipe 38, wherein the liquid supplementing pipe 38 is fixedly arranged at the top end of the liquid storage tank 6; and liquid is replenished to the liquid storage tank through the liquid replenishing pipe.

The utility model discloses an extract purification device for preparing antibody, it is when using the extraction purification device who is used for preparing the antibody to draw the antibody stoste, carry out the ration through first measuring pump and add to the stoste, carry out the ration through back measuring pump and preceding measuring pump and add to the medicament simultaneously, thereby guarantee the accuracy of medicament addition, effectively improve subsequent effect of drawing, improve the damping performance of device through damping spring, carry out prefilter to the antibody stoste through the filter screen, prevent through the limiting plate that the shock attenuation post from deviating from the shock attenuation cover, will deposit through the scraper blade and scrape to the discharge of trash removal department, through footing stabilizing support device, arrange the impurity through arranging the miscellaneous valve control, carry out the fluid infusion through the fluid infusion pipe to the liquid reserve tank, before accomplishing above-mentioned action, at first will remove the position that the user needed.

The utility model discloses an extract purification device for preparing antibody, above the mounting means, the connected mode or the mode that sets up of all parts are common mechanical system to concrete structure, model and the coefficient index of its all parts are its from taking the technique, as long as can reach all can implement of its beneficial effect, so do not add at many and give unnecessary details.

The utility model relates to an extraction and purification device for preparing antibody, under the condition that the contrary explanation is not made, the directional terms "up, down, left, right, front, back, inside, and outside, and vertical and horizontal" included in the term merely represent the orientation of the term in the normal use state, or be a trivial term understood by those skilled in the art, and should not be considered as limiting the term, at the same time, the numerical terms "first," "second," and "third," etc. do not denote any particular quantity or order, but rather are used to distinguish one from another, furthermore, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements, but also includes other elements not expressly listed or inherent to such process, method, article, or apparatus.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.

Claims

1. An extraction and purification device for preparing an antibody comprises a base (1), a mixing drum (2), a liquid outlet pipe (3), a precipitation condensing box (4) and a liquid discharge pipe (5), wherein the mixing drum (2) is fixedly arranged at the top end of the base (1), an inner cavity is arranged inside the mixing drum (2), the liquid outlet pipe (3) is fixedly arranged at the lower side of the mixing drum (2) and communicated with the inner cavity, the precipitation condensing box (4) is positioned at the right side of the mixing drum (2), the right end of the liquid outlet pipe (3) is fixedly connected with the liquid inlet end of the precipitation condensing box (4), and the liquid discharge pipe (5) is fixedly arranged at the bottom end of the precipitation condensing box (4); the device is characterized by further comprising a liquid storage tank (6), a connecting pipe (7), a first metering pump (8), a liquid inlet pipe (9), a bottom plate (10), a machine base (11), a motor (12), a reduction gearbox (13), a rotating shaft (14), a rotating blade (15), a rear support (16), a rear screw pipe (17), a rear screw rod (18), a rear medicament box (19), a rear medicament pipe (20), a rear metering pump (21), a front support (22), a front screw pipe (23), a front screw rod (24), a front medicament box (25), a front medicament pipe (26) and a front metering pump (27), wherein the liquid storage tank (6) is fixedly arranged on the left side of the top end of the mixing cylinder (2), the connecting pipe (7) is fixedly arranged on a liquid outlet of the liquid storage tank (6), the first metering pump (8) is fixedly arranged on the right side of the top end of the mixing cylinder (2), and the right end of the connecting pipe (7) is fixedly connected with, the rear part of the liquid inlet pipe (9) is fixedly connected with the front end of the first metering pump (8), the bottom of the liquid inlet pipe (9) is inserted into the inner cavity from the top end of the mixing cylinder (2), the bottom plate (10) is positioned below the base (1), the base (11) is fixedly arranged on the front side of the top end of the bottom plate (10), the motor (12) is fixedly arranged on the top end of the base (11), the reduction gearbox (13) is fixedly arranged on the rear side of the top end of the bottom plate (10), the rear part of the motor (12) is fixedly connected with the front part of the reduction gearbox (13), the rotating shaft (14) is fixedly arranged on the top end of the reduction gearbox (13), the top end of the rotating shaft (14) penetrates through the base (1) from the top end of the base (1) to be inserted into the inner cavity, the rotating blade (15) is fixedly arranged in the middle part of the rotating shaft (14), the rear support (16) is fixedly arranged on, the rear screw tube (17) is rotatably arranged at the top end of the rear support (16), the bottom end of the rear screw rod (18) is inserted into the top end of the rear screw tube (17) and screwed into the top end of the rear screw tube (17), the top end of the rear screw rod (18) is fixedly connected with the bottom end of the rear medicament box (19), the rear medicament tube (20) is fixedly arranged at the bottom end of the rear medicament box (19), the right part of the rear medicament tube (20) is inserted into the inner cavity from the rear end of the mixing barrel (2), the rear metering pump (21) is fixedly arranged in the middle of the rear medicament tube (20), the front support (22) is fixedly arranged at the left front side of the top end of the base (1), the front screw tube (23) is rotatably arranged at the top end of the front support (22), the bottom end of the front screw rod (24) is inserted into the top end of the front screw tube (23) and screwed into the top end of the front screw tube (23), and the top end of the front screw rod (24) is fixedly connected with the bottom end of the front medicament, the medicine tube (26) that advances sets up in the bottom of preceding medicament case (25) fixedly, the intracavity is inserted into from the front end of mixing drum (2) to the right part of medicine tube (26) that advances, preceding measuring pump (27) are fixed to be set up in the middle part of advancing medicine tube (26).

2. The extraction and purification device for preparing antibody according to claim 1, further comprising a shock absorption column (28), a shock absorption sleeve (29) and a shock absorption spring (30), wherein the shock absorption column (28) is fixedly arranged at the bottom end of the base (1), a shock absorption cavity is arranged inside the shock absorption sleeve (29), the shock absorption spring (30) is fixedly arranged at the bottom end of the shock absorption cavity, and the bottom end of the shock absorption column (28) is inserted into the inner cavity from the top end of the shock absorption sleeve (29).

3. The extraction and purification device for preparing antibody according to claim 2, further comprising a filter screen (31), wherein the filter screen (31) is fixedly arranged at the bottom end of the inner cavity.

4. The extraction and purification device for preparing antibody according to claim 3, further comprising a limiting plate (32), wherein the limiting plate (32) is fixedly arranged at the bottom end of the shock absorption column (28), the bottom end of the limiting plate (32) is contacted with the top end of the shock absorption spring (30), the bottom end of the limiting plate (32) is provided with a limiting tube, the bottom end of the shock absorption sleeve (29) is provided with a limiting rod and is located at the inner end of the shock absorption spring (30), and the top end of the limiting rod is inserted into the bottom end of the limiting tube from the bottom end of the limiting tube.

5. The extraction and purification device for preparing the antibody according to claim 4, further comprising a scraper (33) and a trash removal pipe (34), wherein a cross bar is arranged at the bottom of the rotating shaft (14), the scraper (33) is fixedly arranged at the bottom end of the cross bar, the bottom end of the scraper (33) is in contact with the bottom end of the inner cavity, a trash removal port is arranged at the right side of the bottom end of the inner cavity, and the top end of the trash removal pipe (34) is fixedly connected with the bottom end of the trash removal port.

6. The extraction and purification device for preparing antibody according to claim 5, further comprising a purge valve (35), wherein the purge valve (35) is fixedly arranged in the middle of the purge pipe (34).

7. The extraction and purification device for preparing antibody according to claim 6, further comprising a pillar (36) and a foot (37), wherein the pillar (36) is fixedly arranged at the bottom end of the bottom plate (10), and the foot (37) is fixedly arranged at the bottom end of the pillar (36).

8. The extraction and purification device for preparing antibody according to claim 7, further comprising a solution supplementing pipe (38), wherein the solution supplementing pipe (38) is fixedly arranged at the top end of the liquid storage tank (6).