CN211402140U - Biological chip for zone heating by surface plasma heat effect - Google Patents
Biological chip for zone heating by surface plasma heat effect Download PDFInfo
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- CN211402140U CN211402140U CN201921602416.5U CN201921602416U CN211402140U CN 211402140 U CN211402140 U CN 211402140U CN 201921602416 U CN201921602416 U CN 201921602416U CN 211402140 U CN211402140 U CN 211402140U
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Abstract
The utility model discloses an utilize biochip of surface plasma heat effect subregion heating contains transparent base member, arbitrary one side of transparent base member is equipped with nanometer metal island membrane, and nanometer metal island membrane appearance is island form distribution in the microcosmic, and the yardstick of island is below the micron, and the interval is under the micron between the island, and nanometer metal island membrane upper surface covers or has plated the one deck and be used for providing chemical protection and prevent the coating of fluorescence quenching, and the top of coating is equipped with biological sample layer, the utility model discloses the biochip based on surface plasma heat effect subregion heating promptly has utilized the nanometer metal island membrane that can absorb light energy by force and has heated as the material, under the light source that has the big or small facula of micronano size shines, has realized that the region of generating heat is little, heat source locality is high, characteristics such as non-contact heating.
Description
Technical Field
The utility model relates to a biochip technical field specifically is an utilize biochip of surface plasma fuel factor subregion heating.
Background
Biochips, also called protein chips or gene chips, originate from crystallization combining DNA hybridization probe technology with semiconductor industry technology. The technology is that a large number of probe molecules are fixed on a support and then hybridized with DNA with fluorescent labels or other sample molecules (such as proteins, factors or small molecules), and the quantity and sequence information of the sample molecules are obtained by detecting the hybridization signal intensity of each probe molecule.
The existing biochip aims to integrate biochemical reaction in an area as small as possible, but simultaneously, separate temperature control is often required to be carried out on different partitioned cavities, and inevitable contradiction exists between pursuing high integration level and partitioned heating. Heating by electronic means may be subject to the size limitations of electronic temperature control elements and may not further improve integration.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a biochip of surface plasma fuel effect subregion heating to solve the problem of proposing in the above-mentioned background art.
In order to achieve the above object, the present invention provides the following technical solutions:
the utility model provides an utilize biological chip of surface plasma heat effect subregion heating, contains transparent base member, arbitrary one side of transparent base member is equipped with nanometer metal island membrane, and nanometer metal island membrane is distribution for island form in the microcosmic appearance, and the yardstick of island is below the micron, and the interval is below the micron between the island, and nanometer metal island membrane upper surface covers or plates one deck and is used for providing chemical protection and prevent the coating of fluorescence quenching, and the top of coating is equipped with biological sample layer.
As a further technical solution of the utility model: the nano metal island film is made of nano gold/silver through quenching.
As a further technical solution of the utility model: the coating layer is one of a silicon dioxide layer, an organic glass pmma layer or a pdms layer.
As a further technical solution of the utility model: the thickness of the coating layer is 10nm to 50 nm.
As a further technical solution of the utility model: the transparent substrate is glass.
Compared with the prior art, the beneficial effects of the utility model are that: the utility model discloses biochip based on surface plasma heat effect subregion heating has utilized the nanometer metal island membrane that can absorb light energy by force and heat as the material promptly, under the light source that has the big or small facula of the size of receiving a little shines, has realized that the region that generates heat is little, heat source locality is high, non-contact heats characteristics such as.
Drawings
Fig. 1 is an overall structural view of the present invention.
FIG. 2 is a schematic diagram of island subsections of a nano-metal island film.
In the figure: 1-transparent matrix, 2-coating layer and 3-biological sample layer.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative work belong to the protection scope of the present invention.
Example 1: referring to fig. 1-2, a biochip using surface plasma thermal effect zone heating includes a transparent substrate 1, a nano metal island film is disposed on any side of the transparent substrate 1, the nano metal island film is microscopically distributed in island shape, the size of the island is below micron, the distance between the islands is below micron, a coating layer 2 for providing chemical protection and preventing fluorescence quenching is covered or plated on the upper surface of the nano metal island film, and a biological sample layer 3 is disposed above the coating layer 2. The nano metal island film is made of nano gold/silver through quenching. The coating layer 2 is one of a silicon dioxide layer, an organic glass pma layer or a pdms layer. The thickness of the cladding layer 2 is between 10nm and 50 nm.
The surface plasma resonance has a thermal effect, that is, under the action of irradiation light with a proper frequency, a metal structure can generate strong optical resonance, and along with a strong local electromagnetic field, the local energy of the electromagnetic field can generate the thermal effect, so that the medium in the range of the field intensity can be rapidly heated.
In biochemical experiments where heating and fluorescence detection of products are required, it is common practice to heat with electrical heating elements, irradiate light and collect scattered light from biological samples. According to the invention, the nano metal island film capable of heating is integrated, and the surface plasma heat effect generated by incident light on the island film is utilized to heat the biological sample, so that the characteristics that the incident light scale can be compressed to be very small and the surface plasma heat effect is strong in heat locality are utilized, and the zone heating is realized; and the transmitted light which is not converted into heat is used as the detection light which is incident to the biological sample to carry out the scattered light detection of the biological sample, thereby greatly improving the integration level.
Wherein the composite structure refers to a nano metal island film structure with a glass coating layer 2, and the coating layer 2 has the functions of: 1. protecting the nano metal island film from chemical damage such as oxidation, and forming an isolation layer with a certain distance between the biological sample and the metal island film to prevent fluorescence quenching in some detection needing to detect fluorescence.
Example 2, on the basis of example 1, the thickness of the coating layer 2 is between 10nm and 50nm, the size is nanometer, and the fluorescence of the biological sample can be prevented from being quenched and absorbed by the nanometer metal island film.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (5)
1. The biochip for zone heating by utilizing the thermal effect of the surface plasma comprises a transparent substrate (1), and is characterized in that a nano metal island film is arranged on any one side of the transparent substrate (1), the shape of the nano metal island film is microscopically distributed in an island shape, the size of each island is below micrometers, the distance between the islands is below micrometers, a coating layer (2) used for providing chemical protection and preventing fluorescence quenching is covered or plated on the upper surface of the nano metal island film, and a biological sample layer (3) is arranged above the coating layer (2).
2. The biochip for zone heating by surface plasmon thermal effect according to claim 1, wherein the nano metal island film is made of nano gold/silver by quenching.
3. The biochip for zone heating by surface plasmon thermal effect according to claim 1, wherein the coating layer (2) is one of a silicon dioxide layer, an organic glass pmma layer or a pdms layer.
4. The biochip for zone heating by surface plasmon thermal effect according to claim 3, characterized in that the thickness of the coating (2) is between 10nm and 50 nm.
5. The biochip for zonal heating by surface plasmon thermal effect according to claim 1, wherein the transparent substrate (1) is glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921602416.5U CN211402140U (en) | 2019-09-25 | 2019-09-25 | Biological chip for zone heating by surface plasma heat effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921602416.5U CN211402140U (en) | 2019-09-25 | 2019-09-25 | Biological chip for zone heating by surface plasma heat effect |
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| Publication Number | Publication Date |
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| CN211402140U true CN211402140U (en) | 2020-09-01 |
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| CN201921602416.5U Active CN211402140U (en) | 2019-09-25 | 2019-09-25 | Biological chip for zone heating by surface plasma heat effect |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110596064A (en) * | 2019-09-25 | 2019-12-20 | 宁波赫柏生物科技有限公司 | Biological chip for zone heating by surface plasma heat effect |
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2019
- 2019-09-25 CN CN201921602416.5U patent/CN211402140U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110596064A (en) * | 2019-09-25 | 2019-12-20 | 宁波赫柏生物科技有限公司 | Biological chip for zone heating by surface plasma heat effect |
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