CN211394472U

CN211394472U - Enterovirus typing real-time fluorescence PCR detection kit

Info

Publication number: CN211394472U
Application number: CN201921586352.4U
Authority: CN
Inventors: 杨爱平
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-09-23
Filing date: 2019-09-23
Publication date: 2020-09-01
Anticipated expiration: 2029-09-23

Abstract

The utility model discloses an enterovirus typing real-time fluorescence PCR detection kit, which comprises a detection kit device main body, a kit, a box cover and a control console, wherein the bottom of the detection kit device main body is fixedly connected with the kit, the inner side in the middle of the top end of the kit is embedded and connected with a test tube groove, the other side of the kit is embedded and connected with a refrigerator, the middle of the inner bottom end of the kit is fixedly connected with a storage battery, the two ends of the storage battery are linearly connected with a vibration motor, the vibration motor can effectively accelerate the molecular activity rate in the medicine, thereby improving the mixing quality of the medicine, and is convenient for later-stage experimenters to use and observe the medicine, a tool groove can be convenient for the experimenters to store, carry and take some experimental tools or medicines when in use, a refrigeration groove can be convenient, embodies the utility model discloses a structure protectiveness has extensive use prospect in the future.

Description

Enterovirus typing real-time fluorescence PCR detection kit

Technical Field

The utility model relates to the technical field of biological detection tests, in particular to an enterovirus typing real-time fluorescence PCR detection kit.

Background

The polymerase chain reaction is a molecular biology technique for amplifying and amplifying specific DNA fragments, which can be regarded as special DNA replication in vitro, and the biggest characteristic of PCR is that trace amount of DNA can be greatly increased. Therefore, even if only a single spot of DNA can be isolated from the hairs, skin or blood left by the murder in the murder case, or the remains of the historic creatures and the historical characters in the fossil, the DNA can be amplified by PCR and compared. This is also the strength of the "micro evidence". The inventors first proposed a method of PCR in 1983, and invented the polymerase chain reaction, i.e., the simple DNA amplification method, in 1985, which means that the PCR technique is truly born. By now 2013, PCR has evolved to a third generation technology. In 1973, Taiwan scientist Qianjin discovered stable TaqDNA polymerase, which also made a fundamental contribution to the development of PCR technology.

Current enterovirus typing real-time fluorescence PCR detect reagent box, it does not set up any article parking space except that the test tube itself, thereby lead to the experimenter can't deposit reserve to some auxiliary equipment when using, current enterovirus typing real-time fluorescence PCR detect reagent box is inside not setting up cold-stored structure simultaneously itself, thereby lead to the kit itself can't effectively to its inside medicine save work, and do not set up any supplementary test structure that dispenses in the enterovirus typing real-time fluorescence PCR detect reagent box, thereby the experimenter need consume the plenty of time on the work of dispensing before the detection, and can't ensure the quality of dispensing, reduce the practical degree of kit itself.

Therefore, how to design a real-time fluorescence PCR detection kit for enterovirus typing becomes a problem to be solved currently.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a real-time fluorescence PCR detect reagent box of enterovirus typing to propose in solving above-mentioned background art not have any article parking space, do not have any supplementary experimental structure of dispensing and deposit the relatively poor problem of effect to the medicine.

In order to achieve the above object, the utility model provides a following technical scheme: an enterovirus typing real-time fluorescence PCR detection kit comprises a detection kit device main body, a kit, a box cover and a control console, wherein the bottom of the detection kit device main body is fixedly connected with the kit, the inner side in the middle of the top end of the kit is connected with a test tube groove in an embedded manner, the other side of the kit is connected with a refrigerator in an embedded manner, hydraulic columns are connected with the periphery of the top end of the kit in an embedded manner, a storage battery is fixedly connected with the middle of the inner bottom end of the kit, two ends of the storage battery are linearly connected with a vibration motor, a plurality of test tubes are connected with the inner part of the test tube groove in an embedded manner, the top end of the kit is movably connected with the box cover, movable columns are fixedly connected with the periphery of the top end of the box cover, a test tube cover plate is fixedly, the periphery of the bottom end of the kit is fixedly connected with a base.

Preferably, the inner side of one side of the top end of the kit is connected with a tool groove in an embedded mode, and the inner side of the other side of the top end of the kit is connected with a refrigerating groove in an embedded mode.

Preferably, the top end of the vibration motor is connected with a vibration plate in an overlapping manner.

Preferably, the other side of the inside of the test tube groove is fixedly connected with a separation net.

Preferably, the two sides of the top end of the box cover are fixedly connected with leakage-proof plugs.

Preferably, the kit, the box cover at the top end, the console in the middle of the front surface and the base at the bottom end form a main body of the detection kit device together.

Preferably, the box cover consists of a movable column around the top end, leakage-proof plugs on two sides of the top end, a test tube cover plate in the middle of the top end and a handle in the middle of the bottom end.

Compared with the prior art, the beneficial effects of the utility model are that:

1. this kind of real-time fluorescence PCR detect reagent box of enterovirus typing through the instrument groove, can be convenient for the experimenter to save some experimental tools or medicines and carry work, takes when being convenient for to use, simultaneously through refrigerating the groove, can be convenient for medical personnel deposit some required medicines of experiment, can avoid some medicines to be difficult to deposit under the higher condition of some temperature, easily take place the reaction and lead to the medicine unusable to cause the waste, embodied the utility model discloses a storability.

2. This kind of real-time fluorescence PCR detect reagent box of enterovirus typing, the kit, shock dynamo through its inside setting, the vibrations board that can drive its top shakes, and shake the mixing operation through the medicine of vibrations board in to its top test tube, molecular activity speed in the medicine can effectively be accelerated, thereby improve its mixing quality, the later stage of being convenient for experimenter is to the use and the observation of medicine, effectively avoid the experimenter before experiment detection work, consume a large amount of dispensing time, and can't ensure the quality of dispensing, reduce the practical degree of reagent box itself.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of a part of the structure of the kit according to the present invention;

FIG. 3 is a schematic diagram of a partial cross-sectional structure of the kit according to the present invention;

fig. 4 is a schematic view of the structure of the box cover of the present invention.

In the figure: 1. the device comprises a detection kit device body, 2, a kit, 201, a test tube groove, 202, a tool groove, 203, a refrigeration groove, 204, a refrigerator, 205, a hydraulic column, 206, a storage battery, 207, a vibration motor, 208, a vibration plate, 209, a test tube, 2010, a separation net, 3, a box cover, 301, a movable column, 302, a leakage-proof plug, 303, a test tube cover plate, 304, a carrying handle, 4, a control console, 5 and a base.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative work belong to the protection scope of the present invention based on the embodiments of the present invention.

In a first embodiment, please refer to fig. 1-4, the present invention provides a technical solution: an enterovirus typing real-time fluorescence PCR detection kit comprises a detection kit device main body 1, a kit 2, a kit cover 3 and a control console 4, wherein the bottom of the detection kit device main body 1 is fixedly connected with the kit 2, the inner side in the middle of the top end of the kit 2 is connected with a test tube groove 201 in an embedded manner, the other side of the kit 2 is connected with a refrigerator 204 in the middle of the embedded manner, the periphery of the top end of the kit 2 is connected with a hydraulic column 205 in an embedded manner, the middle of the inner bottom end of the kit 2 is fixedly connected with a storage battery 206, two ends of the storage battery 206 are linearly connected with a vibration motor 207, the interior of the test tube groove 201 is connected with a plurality of test tubes 209 in an embedded manner, the top end of the kit 2 is movably connected with the kit cover 3, the periphery of the top end of, the middle of the front surface of the reagent box 2 is connected with a control console 4 in an embedded mode, and the periphery of the bottom end of the reagent box 2 is fixedly connected with a base 5.

Preferably, the inboard embedding in top one side of kit 2 is connected with tool groove 202, the inboard embedding in top opposite side of kit 2 is connected with refrigeration groove 203, through tool groove 202, the experimenter of can being convenient for stores some experiment instruments or medicines and carries work, take when being convenient for to use, simultaneously through refrigeration groove 203, the medical personnel of can being convenient for deposit some required medicines of experiment, can avoid some medicines to be difficult to deposit under the higher condition of some temperatures, the easy reaction leads to the medicine unusable, thereby cause the waste, embodied the utility model discloses a storability.

Preferably, shock dynamo 207's top overlap joint has vibrations board 208 mutually, at first vibrations board 208 can divide into two parts from top to bottom, the bottom is made for the higher metal material of hardness, can effectively avoid shock dynamo 207 to cause the damage of colliding with of certain degree to vibrations board 208 bottom when vibrations, the top then is made for the better rubber materials of protecting effect, can play and protect spacing effect to test tube 209 bottom, reach the effect that the medicine in the mixed test tube 209 was dispensed to the high efficiency when avoiding vibrations board 208 to cause the damage of certain degree to test tube 209 when vibrations, embodied the utility model discloses a structure protectiveness.

Preferably, the inside opposite side fixedly connected with of test tube groove 201 separates net 2010, through separating net 2010, can play isolated test tube groove 201 and the inside usage space's of refrigeration groove 203 effect, can ensure simultaneously that cold air circulates each other in refrigeration groove 203 and the test tube groove 201, play and carry out cold-stored work of depositing to the inside back medicine that mixes of test tube groove 201, avoid the experimenter to directly handle the experimental medicine that does not use after the experiment finishes, cause the extravagant phenomenon of medicine.

Preferably, the top both sides fixedly connected with leak protection stopper 302 of lid 3, through leak protection stopper 302, can be in the closed back of lid 3, carry out the work of plugging up in instrument groove 202 and the refrigeration groove 203 notch respectively, play the effect of separate space, avoid refrigerator 204 to lead to the fact the damage of certain degree to the metal tool when carrying out the work of refrigerating to instrument in the instrument groove 203, make its metal surface appear fog when taking out the use and take place oxidation phenomenon, also can improve the energy consumption problem of detection kit device main part 1 itself greatly simultaneously, embodied the utility model discloses a practicality.

Preferably, kit 2 and the lid 3 on top, the base 5 of control cabinet 4 and bottom in the middle of the front constitutes jointly and detects reagent box device main part 1, at first the experimenter can put into test tube 201 with the test tube 209 of being equipped with the medicine, and control closed lid 3, later through pressing the shock dynamo control button on the control cabinet 4, start shock dynamo 207, the vibrations board 208 that drives its top through shock dynamo 207 shakes, and shake the mixing operation through the medicine of vibrations board 208 in its top test tube 209, can effectively accelerate the interior molecular activity rate of medicine, thereby improve its mixing quality, be convenient for later stage experimenter is to the use and the observation of medicine, effectively avoid the experimenter before experiment detection work, consume a large amount of dispensing time, and can't ensure the quality of dispensing, reduce the practical degree of kit itself.

Preferably, the box cover 3 is composed of a movable column 301 around the top end, leak-proof plugs 302 at both sides of the top end, a test tube cover plate 303 at the middle of the top end and a handle 304 at the middle of the bottom end, firstly, an experimenter can start the hydraulic column 205 in the reagent box 2 by pressing a box cover control button on the control console 4, the movable column 301 at the inner side of the top end of the box cover is pushed to lift by the hydraulic column 205, so as to play a role of opening the box cover 3, the mode can effectively improve the protection of the box cover 3 to the reagent box 2 when the box cover is closed, and simultaneously, through the test tube cover plate 303 arranged on the box cover 3, the vibrating plate is made of the same material as the top of the vibrating plate 208, and the test tube cover plate 303 is provided with a plurality of through holes corresponding to the shapes of the tube openings of the test tubes 209, so that when the box cover 3 is closed, seal its bottom test tube 209 and carry out the effect of protection to the test tube mouth, further improved the protectiveness of detect reagent box device main part 1 to inside medicine or structure.

The working principle is as follows: firstly, an experimenter can start a hydraulic column 205 in a kit 2 by pressing a box cover control button on a control console 4, and the hydraulic column 205 pushes a movable column 301 on the inner side of the top end of the box cover to lift up, so as to play a role of opening the box cover 3, the mode can effectively improve the protection of the box cover 3 on the kit 2 when being closed, meanwhile, the box cover 3 is provided with a test tube cover plate 303 which is made of the same material as the top of a vibrating plate 208 and is provided with a plurality of through holes corresponding to the shapes of tube openings of test tubes 209, so that the box cover 3 can ensure that the test tubes 209 at the bottom end of the box cover are sealed and protect the test tube openings when being closed, the protection of a detection kit device main body 1 on internal medicines or structures is further improved, meanwhile, the box cover can respectively plug and block the notches of a tool groove 202 and a refrigeration groove 203 after the box cover 3 is closed by a leakage-proof, the effect of space separation is achieved, the metal tools are prevented from being damaged to a certain extent when the refrigerator 204 refrigerates the tools in the tool groove 203, the metal surfaces are oxidized due to fog when the tools are taken out for use, and meanwhile, the problem of energy consumption of the detection kit device main body 1 can be greatly improved, so that the practicability of the utility model is embodied;

then, the experimenter can put the test tube 209 filled with the medicine into the test tube slot 201, and control the closed box cover 3, and then start the vibration motor 207 by pressing the control button of the vibration motor on the console 4, drive the vibration plate 208 at the top end of the vibration motor 207 to vibrate, and vibrate and mix the medicine in the test tube 209 at the top end of the vibration plate 208, so that the movement rate of molecules in the medicine can be effectively accelerated, thereby improving the mixing quality of the medicine, being convenient for the experimenter to use and observe the medicine at the later stage, effectively avoiding the experimenter from consuming a large amount of dispensing time before experimental detection work, and being incapable of ensuring the dispensing quality, and reducing the practical degree of the reagent box;

then, the vibration plate 208 can be divided into an upper part and a lower part, the bottom part is made of a metal material with higher hardness, the bottom end of the vibration plate 208 can be effectively prevented from being collided and damaged to a certain extent by the vibration motor 207 during vibration, the top part is made of a rubber material with better protection effect, the bottom end of the test tube 209 can be protected and limited, the effect of efficiently dispensing and mixing the medicines in the test tube 209 is achieved while the vibration plate 208 is prevented from damaging the test tube 209 to a certain extent during vibration, and the structural protection of the utility model is embodied;

next, the separation net 2010 can isolate the use spaces in the test tube groove 201 and the refrigeration groove 203, and simultaneously can ensure that the cold air in the refrigeration groove 203 and the cold air in the test tube groove 201 are mutually circulated, so that the cold storage and storage work of the mixed medicines in the test tube groove 201 is performed, and the phenomenon that the unused experimental medicines are directly processed by experimenters after the experiment is finished and the medicine waste is caused is avoided;

finally, through tool groove 202, can be convenient for the experimenter to save some experimental tools or medicines and carry work, take when being convenient for to use, simultaneously through refrigerating groove 203, can be convenient for medical personnel to deposit some required medicines of experiment, can avoid some medicines to be difficult to deposit under the higher condition of some temperature, the reaction of easily taking place leads to the medicine unusable to cause the waste, embodied the utility model discloses a storability, this is exactly this kind of enterovirus typing real-time fluorescence PCR detect reagent box's theory of operation.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides an enterovirus typing real-time fluorescence PCR detect reagent box, includes detect reagent box device main part (1), kit (2), lid (3) and control cabinet (4), its characterized in that: the bottom of the detection kit device main body (1) is fixedly connected with a kit (2), the inner side of the middle of the top end of the kit (2) is connected with a test tube groove (201) in an embedded manner, the middle of the other side of the kit (2) is connected with a refrigerator (204) in an embedded manner, hydraulic columns (205) are connected around the top end of the kit (2) in an embedded manner, a storage battery (206) is fixedly connected in the middle of the inner bottom end of the kit (2), two ends of the storage battery (206) are linearly connected with a vibration motor (207), a plurality of test tubes (209) are connected in the embedded manner in the test tube groove (201), a box cover (3) is movably connected at the top end of the kit (2), movable columns (301) are fixedly connected around the top end of the box cover (3), a test tube cover plate (303) is fixedly connected in the middle of the top, the middle of the front surface of the kit (2) is connected with a console (4) in an embedded mode, and the periphery of the bottom end of the kit (2) is fixedly connected with a base (5).

2. The enterovirus typing real-time fluorescence PCR detection kit according to claim 1, which is characterized in that: the inner side of one side of the top end of the kit (2) is connected with a tool groove (202) in an embedded mode, and the inner side of the other side of the top end of the kit (2) is connected with a refrigerating groove (203) in an embedded mode.

3. The enterovirus typing real-time fluorescence PCR detection kit according to claim 1, which is characterized in that: the top end of the vibration motor (207) is connected with a vibration plate (208) in an overlapping way.

4. The enterovirus typing real-time fluorescence PCR detection kit according to claim 1, which is characterized in that: the other side in the test tube groove (201) is fixedly connected with a separation net (2010).

5. The enterovirus typing real-time fluorescence PCR detection kit according to claim 1, which is characterized in that: the two sides of the top end of the box cover (3) are fixedly connected with leakproof plugs (302).

6. The enterovirus typing real-time fluorescence PCR detection kit according to claim 1, which is characterized in that: the kit (2), a box cover (3) at the top end, a console (4) in the middle of the front surface and a base (5) at the bottom end jointly form a detection kit device main body (1).

7. The enterovirus typing real-time fluorescence PCR detection kit according to claim 1, which is characterized in that: the box cover (3) is composed of movable columns (301) around the top end, leakage-proof plugs (302) on two sides of the top end, a test tube cover plate (303) in the middle of the top end and a handle (304) in the middle of the bottom end.