CN211392349U - Kit for extracting bacterial plasmid DNA by silica gel column method - Google Patents

Abstract

The utility model discloses a silica gel column method bacterium plasmid DNA draws kit, comprises a box bod, the top of box body articulates there is the lid, and the inside of box body is separated for first inner chamber, second inner chamber and third inner chamber through first interlayer and second interlayer, and fixed interlude is connected with a plurality of heat conduction section of thick bamboo on the first interlayer, and the inside of second inner chamber is all arranged in to a plurality of heat conduction section of thick bamboo, and sealed thermal-insulated cover is installed to the equal block in top of every heat conduction section of thick bamboo, and cold water has been annotated to the inside of second inner chamber, and the inside of third inner chamber is fixed respectively and is equipped with cooling box, water pump and seal box, the beneficial effects of the utility model are that: can ensure through cold water and heat conduction section of thick bamboo heat transfer that the inside temperature of heat conduction section of thick bamboo is lower, prolong the cycle of depositing of reagent pipe, can automatic control solenoid valve and water pump work through temperature detect switch, can make cold water get up through cooling case circulation, through ice bag and water direct contact heat transfer, cool down the water, ensure the temperature invariant, improve the low temperature effect.

Description

Kit for extracting bacterial plasmid DNA by silica gel column method

Technical Field

The utility model relates to a kit, in particular to silica gel column method bacterium plasmid DNA draws kit belongs to kit technical field.

Background

The box is used for containing chemical reagents for detecting chemical components, drug residues, virus types and the like. Is generally used by hospitals and pharmaceutical enterprises. The separation principle of silica gel chromatography is to separate substances according to different adsorption forces of the substances on silica gel, in general, substances with larger polarity are easy to be adsorbed by the silica gel, substances with weaker polarity are not easy to be adsorbed by the silica gel, and the whole chromatography process is the adsorption, desorption, re-adsorption and re-desorption processes.

The existing silica gel column method bacterial plasmid DNA extraction kit does not have a low-temperature storage structure, greatly influences the storage period, is not convenient for remote transportation, has poor damping capacity, and easily causes the breakage of reagent tubes in the transportation process to cause loss.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a silica gel column method bacterium plasmid DNA draws kit to solve the current silica gel column method bacterium plasmid DNA that proposes in the above-mentioned background art and draw the kit and do not possess low temperature storage structure and the poor problem of shock-absorbing capacity.

In order to achieve the above object, the utility model provides a following technical scheme: a bacterial plasmid DNA extraction kit based on a silica gel column method comprises a kit body, wherein the top end of the kit body is hinged with a kit cover, the interior of the kit body is divided into a first inner cavity, a second inner cavity and a third inner cavity through a first interlayer and a second interlayer, a plurality of heat conduction cylinders are fixedly and alternately connected to the first interlayer, the plurality of heat conduction cylinders are all arranged in the second inner cavity, the top end of each heat conduction cylinder is clamped and installed with a sealed heat insulation cylinder cover, cold water is injected into the second inner cavity, a cooling box, a water pump and a sealing box are respectively and fixedly arranged in the third inner cavity, a storage net is fixedly arranged in the cooling box, the top of one side of the cooling box is communicated with one side of the bottom end of the second inner cavity through a water inlet pipe, an electromagnetic valve is fixedly installed on the water inlet pipe, and the bottom end of the other side of the cooling box is communicated with the water, and the water outlet end of the water pump is communicated with the other side of the second inner cavity through a water outlet pipe.

As a preferred technical scheme of the utility model, the avris of third inner chamber articulates there is the box door, the outside fixed mounting of box door has temperature detect switch, temperature detect switch's the fixed inside that sets up at the second inner chamber of probe, just temperature detect switch's the position of probe is corresponding with the position of inlet tube, the fixed battery that is equipped with in inside of seal box, water pump and solenoid valve are all through temperature detect switch and battery electric connection.

As a preferred technical scheme of the utility model, every the bottom of heat conduction section of thick bamboo inner wall and the bottom of every thermal-insulated cover all through spring fixedly connected with slider, every the inner wall of heat conduction section of thick bamboo is all fixed and is equipped with heat conduction silica gel layer.

As an optimized technical scheme of the utility model, adjacent two equal fixedly connected with a plurality of heat conduction stick between the heat conduction section of thick bamboo.

As an optimal technical proposal of the utility model, the ice bag is placed on the object placing net.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model relates to a silica gel column method bacterium plasmid DNA extraction kit can ensure that the temperature inside the heat conduction cylinder is lower through the heat exchange of cold water and the heat conduction cylinder, prolongs the storage period of reagent pipes, can automatically control the work of a solenoid valve and a water pump through a temperature control switch, can enable the cold water to circularly flow through a cooling box, and cools the water body through the direct contact heat exchange of an ice bag and the water body, ensures constant water temperature, improves low-temperature effect, can not cause large-area cold air dissipation when taking and placing the reagent pipes, and is more practical; the reagent tube can be tightly pressed by matching the spring with the sliding block, so that the reagent tube is in an elastic stable state, the damping capacity is improved by matching with the heat-conducting silica gel layer, and the reagent tube is protected from being damaged in the transportation process.

Drawings

Fig. 1 is a schematic front structural view of the present invention;

fig. 2 is a schematic sectional view of the present invention.

In the figure: 1. a box body; 2. a box cover; 3. a first barrier layer; 4. a second barrier layer; 5. a first lumen; 6. a second lumen; 7. a third lumen; 8. a heat conducting tube; 9. a heat-insulating cylinder cover; 10. a cooling box; 11. a water pump; 12. a sealing box; 13. a water inlet pipe; 14. an electromagnetic valve; 15. a water outlet pipe; 16. a storage net; 17. a box door; 18. a temperature control switch; 19. a storage battery; 20. a heat conducting rod; 21. a heat conductive silica gel layer; 22. a spring; 23. a slide block.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-2, the utility model provides a silica gel column method bacteria plasmid DNA extraction kit, comprising a box body 1, a box cover 2 hinged on the top of the box body 1, the inside of the box body 1 is divided into a first inner chamber 5, a second inner chamber 6 and a third inner chamber 7 by a first interlayer 3 and a second interlayer 4, the first interlayer 3 is fixedly connected with a plurality of heat conduction cylinders 8 in an inserting way, the plurality of heat conduction cylinders 8 are all arranged in the second inner chamber 6, the top of each heat conduction cylinder 8 is clamped with a sealed heat insulation cylinder cover 9, the inside of the second inner chamber 6 is filled with cold water, the temperature in the heat conduction cylinders 8 can be ensured to be lower by the heat exchange between the cold water and the heat conduction cylinders 8, the storage period of reagent tubes is prolonged, a cooling box 10, a water pump 11 and a sealing box 12 are respectively fixed in the third inner chamber 7, a storage net 16 is fixed in the cooling box 10, the top of one side of the cooling box 10 is communicated with one side of the bottom of the second, an electromagnetic valve 14 is fixedly installed on the water inlet pipe 13, the bottom end of the other side of the cooling box 10 is communicated with the water inlet end of the water pump 11 through a connecting pipe, and the water outlet end of the water pump 11 is communicated with the other side of the second inner cavity 6 through a water outlet pipe 15.

Preferably, the lateral side of the third inner cavity 7 is hinged with a box door 17, a temperature control switch 18 is fixedly installed on the outer side of the box door 17, a probe of the temperature control switch 18 is fixedly arranged inside the second inner cavity 6, the position of the probe of the temperature control switch 18 corresponds to the position of the water inlet pipe 13, a storage battery 19 is fixedly arranged inside the seal box 12, the water pump 11 and the electromagnetic valve 14 are both electrically connected with the storage battery 19 through the temperature control switch 18, the temperature is automatically controlled through the temperature control switch 18, the energy is saved, manual control is not needed, the bottom end of the inner wall of each heat conduction cylinder 8 and the bottom end of each heat insulation cylinder cover 9 are both fixedly connected with a sliding block 23 through springs 22, the inner wall of each heat conduction cylinder 8 is fixedly provided with a heat conduction silica gel layer 21, the reagent tube can be pressed through the cooperation of the springs 22 and the sliding blocks 23, so that, the protection reagent pipe does not receive the damage, and equal fixedly connected with a plurality of heat conduction stick 20 between two adjacent heat conduction section of thick bamboo 8 can increase heat transfer area through heat conduction stick 20, ensures cooling efficiency then, puts and has placed the ice bag on the thing net 16, through ice bag and water direct contact heat transfer, cools down the water, and the rethread water pump 11 realizes the circulation flow of water, ensures that the temperature is invariable.

When in specific use, the utility model relates to a silica gel column method bacterium plasmid DNA draws kit, when needing long-range transportation, at first open thermal-insulated cover 9, arrange the reagent pipe respectively in the inside of a plurality of heat-conducting tube 8, then cover thermal-insulated cover 9, open box door 17 simultaneously and place the ice bag on putting thing net 16, close box door 17 and can set for the temperature limit value for 8010F's temperature control switch 18, when the temperature is higher than the limit value, open water pump 11 and solenoid valve 14 through temperature control switch 18, the water flow in the inside of second inner chamber 6 after solenoid valve 14 opens is to the inside of cooling box 10 and directly contacts with the ice bag heat transfer and cool down, then flow to the inside of second inner chamber 6 again through outlet pipe 15 under the effect of water pump 11, then realize the purpose of low temperature storage, can ensure through the heat transfer of cold water and heat-conducting tube 8 that the temperature in the heat-conducting tube 8 is lower, prolong the storage cycle of reagent pipe, can automatic control solenoid valve 14 and water pump 11 work through temperature detect switch 18, can make cold water get up through cooling box 10 circulation flow, through ice bag and water direct contact heat transfer, cool down the water, ensure the temperature invariant, improve the low temperature effect, second inner chamber 6 separates with first inner chamber 5 is thermal-insulated simultaneously, can not cause the air conditioning of large tracts of land to scatter and disappear when getting the reagent pipe, and is more practical, in the transportation, cooperate through spring 22 and slider 23 and can compress tightly the reagent pipe, make the reagent pipe be in the elastic stability state, cooperate with heat conduction silica gel layer 21 and improve the shock-absorbing capacity, the at utmost protection reagent pipe does not receive the damage in the transportation.

In the description of the present invention, it should be understood that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.

In the present invention, unless otherwise explicitly specified or limited, for example, it may be fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; they may be directly connected or indirectly connected through an intermediate medium, and may be connected through the inside of two elements or in an interaction relationship between two elements, unless otherwise specifically defined, and the specific meaning of the above terms in the present invention will be understood by those skilled in the art according to specific situations.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (5)

1. A bacterial plasmid DNA extraction kit based on a silica gel column method comprises a box body (1) and is characterized in that a box cover (2) is hinged to the top end of the box body (1), the interior of the box body (1) is divided into a first inner cavity (5), a second inner cavity (6) and a third inner cavity (7) through a first interlayer (3) and a second interlayer (4), a plurality of heat-conducting cylinders (8) are fixedly inserted and connected onto the first interlayer (3), the plurality of heat-conducting cylinders (8) are arranged inside the second inner cavity (6), a sealed heat-insulating cylinder cover (9) is clamped and installed at the top end of each heat-conducting cylinder (8), cold water is injected into the second inner cavity (6), a cooling box (10), a water pump (11) and a sealing box (12) are fixedly arranged inside the third inner cavity (7), a storage net (16) is fixedly arranged inside the cooling box (10), the top of cooling case (10) one side is passed through inlet tube (13) and is communicated with one side of second inner chamber (6) bottom, fixed mounting has solenoid valve (14) on inlet tube (13), the bottom of cooling case (10) opposite side is through the end intercommunication of intaking of connecting pipe and water pump (11), the play water end of water pump (11) is through the opposite side intercommunication of outlet pipe (15) and second inner chamber (6).

2. The kit for extracting bacterial plasmid DNA by silica gel column method according to claim 1, wherein: the avris of third inner chamber (7) articulates there is box door (17), the outside fixed mounting of box door (17) has temperature detect switch (18), the fixed inside that sets up in second inner chamber (6) of probe of temperature detect switch (18), just the position of the probe of temperature detect switch (18) is corresponding with the position of inlet tube (13), the inside of seal box (12) is fixed and is equipped with battery (19), water pump (11) and solenoid valve (14) are all through temperature detect switch (18) and battery (19) electric connection.

3. The kit for extracting bacterial plasmid DNA by silica gel column method according to claim 1, wherein: every the bottom of heat conduction section of thick bamboo (8) inner wall and the bottom of every thermal-insulated cover (9) all through spring (22) fixedly connected with slider (23), every the inner wall of heat conduction section of thick bamboo (8) all is fixed and is equipped with heat conduction silica gel layer (21).

4. The kit for extracting bacterial plasmid DNA by silica gel column method according to claim 1, wherein: a plurality of heat conducting rods (20) are fixedly connected between every two adjacent heat conducting cylinders (8).

5. The kit for extracting bacterial plasmid DNA by silica gel column method according to claim 1, wherein: an ice bag is placed on the storage net (16).

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