CN211367528U

CN211367528U - Magnetic rod sleeve suitable for sample component extraction and sample component extraction instrument

Info

Publication number: CN211367528U
Application number: CN201921420841.2U
Authority: CN
Inventors: 杨晓林
Original assignee: Shanghai Fanji Biotechnology Co ltd
Current assignee: Shanghai Fanji Biotechnology Co ltd
Priority date: 2019-08-29
Filing date: 2019-08-29
Publication date: 2020-08-28
Anticipated expiration: 2029-08-29

Abstract

The utility model discloses a magnetic rod sleeve suitable for sample component extraction and a sample component extraction instrument, wherein the magnetic rod sleeve comprises a pipe body, and the outer surface of the pipe body is an uneven surface; the uneven outer surface is a bulge relative to the outer surface of the pipe body, and the bulge comprises a spiral bulge, a sawtooth bulge and an intermittent annular bulge; the sample component extraction instrument comprises a magnetic rod sleeve provided by the utility model; the utility model provides a can intensive mixing sample, make the magnetic rod cover and the sample composition that sample yield is high and efficient sample composition draws draw the appearance.

Description

Magnetic rod sleeve suitable for sample component extraction and sample component extraction instrument

Technical Field

The utility model belongs to the technical field of biotechnology and specifically relates to a bar magnet cover and sample composition extraction appearance suitable for sample composition extraction is related to.

Background

The sample component extractor is an instrument which automatically finishes the work of extracting and purifying specific components or certain substances of various samples by using matched extraction reagents. The components refer to nucleic acid, protein or other molecular substances, and the like, and the sample component extractor is widely applied to various fields of disease control centers, clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, animal husbandry, molecular biology research, chemical engineering, material science, and the like. One of the common methods for extracting components (e.g., nucleic acids) from a sample is to use magnetic beads as carriers, and to use the principle that magnetic beads adsorb a specific substance (e.g., nucleic acids) under a certain condition and separate from the substance under another condition, and then to move the magnetic beads or transfer liquid to realize the whole process of extracting and purifying the corresponding substance. Due to the uniqueness of the principle, the device can be designed into various fluxes, can be used for extracting a plurality of samples through a single tube or simultaneously, is simple and quick to operate, only needs 10-45min for extracting a batch of samples, greatly improves the experimental efficiency, is low in cost, can be used in different laboratories, and is a mainstream sample component extraction instrument in the current market.

Chinese patent publication No. CN201810429777.8, a magnetic rod sleeve, discloses a magnetic rod sleeve, which comprises a substrate, wherein the substrate is provided with a plurality of sleeves capable of being inserted into the tapered portions of PCR reaction tubes, the sleeves are perpendicular to the substrate, each sleeve corresponds to a hole site of a PCR reaction tube, one section of the sleeve connected with the substrate is open, and the other end of the sleeve is closed. The utility model has the advantages of can match with current antithetical couplet row PCR reaction tube, carry out the magnetic bead method to antithetical couplet row PCR reaction tube and draw the nucleic acid operation.

However, the prior art still has the following defects: in the process of extracting the components of the sample, the magnetic rod sleeve is not easy to uniformly mix the sample, so that the problems of low yield of the extracted sample, low purity, increased experiment time cost and the like are caused.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model provides a can the intensive mixing sample, make the magnetic rod cover and the sample composition extraction appearance that sample yield is high and efficient sample composition draws.

The utility model discloses a first aspect provides a bar magnet cover suitable for sample composition draws, and inside can hold corresponding magnet, include: the outer surface of the pipe body is an uneven outer surface; uneven surface has increased the area of contact of body with the sample liquid, and the mixing sample that can be better when the body removes in the sample liquid also makes the magnetic bead disperse evenly in the sample to make the body evenly adsorb the magnetic bead.

Preferably, the utility model discloses a bar magnet cover suitable for sample composition draws still includes the pipe support, be equipped with the through-hole on the pipe support, the body upper end is passed through the through-hole sets up on the pipe support.

Preferably, the uneven outer surface is a protrusion corresponding to the outer surface of the pipe wall, and the protrusion comprises a spiral protrusion, a saw-tooth protrusion, an intermittent annular protrusion and an annular protrusion.

Preferably, the height of the projections is 0.1-40 mm; further preferably, the height of the projection is 0.3-12 mm; a large number of comparison experiments show that the height of the convex part is 0.3-12mm, and the sample can be well mixed.

Preferably, the non-smooth outer surface further comprises a groove relative to the outer surface of the tube wall, and further preferably, the non-smooth outer surface comprises a plurality of individual spherical grooves.

Preferably, the spherical groove is 1/6-1/2 spherical groove; further preferably, the spherical groove is 1/4-1/3 spherical groove; through a large amount of contrast experiments, the groove within the parameter range can realize the effects that in the sample component extraction process, the magnetic beads are not easy to fall off during adsorption and are stably released during release.

Preferably, the protrusion is arranged at the lower part of the pipe body; the groove is formed in the upper portion of the pipe body.

The second aspect of the utility model provides a sample composition draws appearance, include the utility model discloses a bar magnet cover.

The utility model discloses technical scheme is for prior art's beneficial effect: the magnetic rod sleeve of the utility model designs the bulge and the groove with special shape and size by changing the outer surface of the pipe body, so that the magnetic rod sleeve can better mix the sample when moving up and down in the sample liquid, and the sample is more uniformly and comprehensively adsorbed by the magnetic beads; and at the in-process that the magnetic bead shifted, the magnetic bead can better with the utility model discloses a recess combination makes the magnetic bead be difficult for droing, at magnetic bead release in-process, because the restriction of the shape and the size of recess, the phenomenon that the magnetic bead does not drop can not appear yet.

Drawings

Fig. 1 is a side view of a magnetic sleeve suitable for sample component extraction in an embodiment of the invention;

fig. 2 is a top view of a magnetic sleeve suitable for sample component extraction in an embodiment of the present invention;

fig. 3 is a side view of a first tube of a sheath for sample component extraction in an embodiment of the invention;

fig. 4 is a top view of a second tube of a magnetic sleeve suitable for sample component extraction in an embodiment of the present invention;

fig. 5 is a top view of a third tube of a magnetic sleeve suitable for sample component extraction in an embodiment of the present invention;

fig. 6 is a top view of a fourth tube of a sheath for a magnetic rod suitable for sample component extraction in an embodiment of the present invention;

FIG. 7 is a side view of a fifth embodiment of a magnetic rod sleeve for sample component extraction in accordance with embodiments of the present invention;

in the drawings: 1. the pipe body, 2, pipe support, 3, arch, 3.1, heliciform arch, 3.2, zigzag arch, 3.3, discontinuous annular arch, 3.4, annular arch, 4, recess.

The specific implementation mode is as follows:

the present invention will be further described with reference to the accompanying drawings and specific embodiments, but the present invention is not limited thereto.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

The annular protrusion of the present invention, as shown in fig. 6, has an annular protrusion outside the tube body.

Discontinuous annular protruding, as shown in fig. 5, have an annular protruding outside the body, but annular protruding is equipped with the breach all around to liquid down flows from last through the breach.

The spiral pitch of the spiral protrusion of the present invention, as shown in fig. 3, is two spiral pitches L1.

The distance between the saw teeth of the saw tooth shaped protrusion of the present invention is shown in fig. 4, which is the maximum distance L2 between two saw teeth.

The bellied width of being interrupted of discontinuous annular, as shown in fig. 5, the bellied bore L3 of breach of annular.

The most horizontal distance of highly being protruding to the pipe wall of bellying.

The diameter of the spherical groove in the utility model is the diameter R of the sphere represented by the spherical groove.

The magnet of the utility model is various objects with magnetic characteristics.

Referring to fig. 1 and 2, a structure diagram of a magnetic rod sleeve for extracting sample components is shown, wherein the magnetic rod sleeve comprises a pipe body 1, a pipe frame 2 and a protrusion 3; the bottom of body 1 is equipped with arch 3, is equipped with the through-hole on the pipe support 2, and body 2 upper end passes through-hole detachably to be fixed on pipe support 2.

In this embodiment, the pipe body 1 is a circular plastic pipe, but in practical application, the pipe body 1 is not limited to a plastic material, and the pipe diameter is 5 mm.

In this embodiment, the protrusions 3 may have a regular shape, such as a zigzag shape or a spiral shape; but may be irregular and arbitrary.

In this embodiment, the through-hole of

body

1 and 2 junctions of pipe support is the draw-in groove, is equipped with the screens of placing the removal of body 1.

Example 1

Referring to fig. 3, a first tubular structure of a magnetic rod sleeve for sample component extraction is shown, wherein the first tubular structure comprises a tubular body 1 and a spiral protrusion 3.1; the spiral protrusion 3.1 is arranged at the bottom of the tube body 1.

In this embodiment, the spiral protrusion 3.1 is a regular spiral body, the spiral pitch of the spiral protrusion 3.1 is 2mm, and the height is 0.5 mm.

Example 2

Referring to fig. 4, a second tubular structure of a magnetic rod sleeve for sample component extraction is shown, wherein the second tubular structure comprises a tubular body 1 and sawtooth-shaped protrusions 3.2; the sawtooth projections 3.2 are arranged at the bottom of the tube body 1.

In this embodiment, the saw-tooth protrusions 3.2 are regular saw-teeth, and the saw-tooth pitch of the saw-tooth protrusions 3.2 is 1mm, and the height is 0.5 mm.

Example 3

Fig. 5 shows a structure diagram of a third tube of a magnetic rod sleeve for sample component extraction, which includes a tube 1 and an interrupted annular protrusion 3.3; the discontinuous annular bulge 3.3 is arranged at the bottom of the pipe body 1.

In this embodiment, the discontinuous annular protrusion 3.3 is regular, and the discontinuous width of the discontinuous annular protrusion 3.3 is 1mm and the height is 0.5 mm.

Example 4

Referring to fig. 6, a fourth tube structure of a magnetic rod sleeve for sample component extraction is shown, wherein the fourth tube structure comprises a tube 1 and an annular protrusion 3.4; the annular projection 3.4 is arranged at the bottom of the tube body 1.

In this embodiment, the annular protrusion 3.4 is regular in shape and 0.5mm in height.

Example 5

Fig. 7 shows a structure diagram of a fourth tube of the magnetic rod sleeve for sample component extraction, wherein the fourth tube comprises a tube 1, a groove 4, and a spiral protrusion 3.1; recess 4, the protruding 3.1 of heliciform all set up on the outer wall of body 1, and the protruding 3.1 setting of heliciform is in the lower part, and recess 4 sets up on upper portion.

In this embodiment, there are a plurality of grooves 4, the plurality of grooves 4 are uniformly distributed on the outer wall of the tube body 1, and the grooves 4 are 1/4 spherical grooves; the diameter of the groove 4 is 0.5 mm.

Example 6

The following experiments were performed using the magnetic rod sets of examples 1-5 and commercially available magnetic rod sets.

First, optimum mixing time experiment

The most suitable mixing experiment was performed using the magnetic rod sleeves of examples 1-5 and commercially available magnetic rod sleeves, and the experimental procedure was as follows:

1) the same EDTA anticoagulated pig whole blood sample is divided into 3 rounds of experiments (different mixing time), each round is provided with 5 groups, each group comprises 10 parts, and each part comprises 200 microliter of whole blood.

2) The method is characterized in that a blood genome DNA extraction kit (a magnetic bead method) is matched with a full-automatic nucleic acid extractor for extraction (provided by Shanghai Van Ji bioscience Co., Ltd., a reagent product number FG329 and an instrument product number FG48), when parameters are set, on the basis of standard operation setting required by products, the mixing time of a first step digestion and cracking link in different groups is set to be 5min, 1min and 3min, the other parameters are consistent, and the mixing speed of the step is uniformly set to be 700 mm/min.

3) The DNA automatically extracted by the instrument is detected by an ultramicro spectrophotometer, the concentration and the purity standard-reaching rate are calculated, and the purity standard-reaching rate is calculated according to the proportion of samples with OD values 260/280 being more than 1.6 and 260/230 being more than or equal to 2.0 or less than 0 in the total samples.

4) Comparative analysis of each group of data

The results of the experiment are shown in table 1 below:

TABLE 1 comparison of the results of different mixing times for the respective products

As shown in Table 1, when the time for mixing is 5min, the mixing effect of the examples 1-5 and the commercial products is good, and the purity standard reaching rate of the extracted DNA after mixing is 100%; when the mixing time is 3min, the mixing effect of the embodiments 1-5 is good, the purity standard-reaching rate of the extracted DNA after mixing is 90-100%, the mixing effect of the commercially available product is poor, and the purity standard-reaching rate of the extracted DNA after mixing is 60%; when the mixing time is 1min, the purity standard-reaching rate of the DNA extracted after the mixing of the embodiments 1-5 is 60-90%, the mixing effect of the commercially available products is poor, and the purity standard-reaching rate of the DNA extracted after the mixing is 10%; therefore, the magnetic rod sleeve provided by the embodiments 1 to 5 can realize short-time uniform mixing, has a good uniform mixing effect within 3 minutes, can greatly improve the scientific research efficiency, and saves the time cost.

Second, experiment of optimum mixing speed

2) The method is characterized in that a blood genome DNA extraction kit (a magnetic bead method) is matched with a full-automatic nucleic acid extractor for extraction (provided by Shanghai Van Ji bioscience Co., Ltd., a reagent product number FG329 and an instrument product number FG48), when parameters are set, on the basis of standard operation setting required by products, the mixing speed of a first step digestion and cracking link in different groups is set to be 700mm/min, 350mm/min and 100mm/min, other parameters are consistent, and the mixing time in the step is set to be 5min uniformly.

4) Comparative analysis of each group of data

The results of the experiment are shown in table 2 below:

TABLE 2 comparison of the results of different mixing speeds for the respective products

As shown in Table 2, when the mixing speed is 700mm/min, the mixing effect of the examples 1-5 and the commercial products is good, and the purity standard reaching rate of the extracted DNA after mixing is 100%; when the mixing speed is 350mm/min, the mixing effect of the embodiments 1-5 is good, the standard reaching rate of the DNA purity extracted after mixing is 90-100%, the mixing effect of the commercially available product is poor, and the standard reaching rate of the DNA purity extracted after mixing is 60%; when the mixing speed is 100mm/min, the mixing effect of the embodiments 1-5 is good, the standard reaching rate of the DNA purity extracted after mixing is 70-100%, the mixing effect of the commercially available product is poor, and the standard reaching rate of the DNA purity extracted after mixing is 20%; therefore, the magnetic rod sleeve provided by the embodiments 1 to 5 can realize low-speed mixing, has a good mixing effect at 100mm/min, and can avoid the problems of damage to products on a high-speed mixing belt and the like.

The above is only a preferred embodiment of the present invention, and not intended to limit the scope of the invention, and it should be appreciated by those skilled in the art that various equivalent substitutions and obvious changes made in the specification and drawings should be included within the scope of the present invention.

Claims

1. The utility model provides a bar magnet cover suitable for sample composition draws, inside can hold corresponding magnet, its characterized in that includes: the outer surface of the pipe body is an uneven surface; the uneven outer surface is a bulge relative to the outer surface of the pipe body, and the bulge comprises a spiral bulge, a sawtooth bulge and an intermittent annular bulge.

2. The bar magnet sleeve of claim 1 wherein the height of the boss is 0.1-40 mm.

3. The bar magnet sleeve of claim 2 wherein the height of the boss is 0.3-12 mm.

4. The utility model provides a bar magnet cover suitable for sample composition draws, inside can hold corresponding magnet, its characterized in that includes: the outer surface of the pipe body is an uneven surface; the non-smooth outer surface includes a groove relative to the outer surface of the tubular body.

5. The sleeve of claim 4 wherein said recess comprises a plurality of individual spherical recesses.

6. The magnet bar sleeve of claim 5 wherein said spherical recess is 1/6-1/2 spherical recess.

7. A sample component extraction apparatus comprising a magnetic rod cover according to any one of claims 1 to 6.