CN211347519U - Precise trace water sample collection test element for ATP fluorescence detection - Google Patents
Precise trace water sample collection test element for ATP fluorescence detection Download PDFInfo
- Publication number
- CN211347519U CN211347519U CN201922229682.4U CN201922229682U CN211347519U CN 211347519 U CN211347519 U CN 211347519U CN 201922229682 U CN201922229682 U CN 201922229682U CN 211347519 U CN211347519 U CN 211347519U
- Authority
- CN
- China
- Prior art keywords
- sampling
- puncture needle
- reagent
- connecting part
- fluorescence detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The utility model belongs to biological detection consumptive material field, concretely relates to accurate trace water sample collection test element for ATP fluorescence detection, the problem that the collection volume accuracy and the uniformity of solving current water sample test element are not high. Comprises a spring cap, a puncture needle, a connector, a sampling rod and a reaction tube; the puncture needle sleeve is arranged inside the spring cap, and the head of the puncture needle is contacted with the top of the spring structure; the tail part of the puncture needle is contacted with the connecting body; the connecting body comprises a connecting part, a siphon pipe sleeved in the connecting part and a protective film sealed on one end face of the connecting part; the tail part of the puncture needle is contacted with the protective film; the sampling stick is located the reaction tube, and the one end of sampling stick is connected with the bottom of siphon, and the other end of sampling stick is the sampling end. Utilize the utility model discloses when gathering the examination sub-sampling, convenient operation can gather trace liquid, and the liquid acquisition accuracy is high to the uniformity is high.
Description
Technical Field
The utility model belongs to the biological detection consumptive material field specifically relates to a ATP fluorescence detection test that can gather liquid sample.
Background
ATP, Adenosine Triphosphate (Adenosine Triphosphate), is a direct source of energy required for vital activities of all tissue cells in the body. ATP and luciferin can be subjected to oxidation reaction under the catalysis of luciferase to form oxyluciferin and emit fluorescence, and the pollution amount of microorganisms can be determined by testing the fluorescence intensity. At present, the ATP fluorescence detection method is a health monitoring method appointed by the official authorities of the national health and food and drug supervision departments, and is appointed by the nation to be used for evaluating the object surface and water sample cleaning effect, the health condition and the like in the fields of food and drug processing, storage and transportation, trade, catering service, medical treatment and the like.
The ATP fluorescence detection requires sampling by using a swab, and the structure of the ATP fluorescence detection swab is mainly divided into two types according to different sampling objects: a cotton swab for collecting a sample from a surface of an object; and a water sample swab for collecting the liquid sample. The amount of the liquid sample collected by the water sample tester is crucial to the accuracy and consistency of the detection result. Therefore, the development of an ATP fluorescence detection swab capable of improving the accuracy and consistency of the amount of liquid to be sampled is still expected in the market.
SUMMERY OF THE UTILITY MODEL
For the purpose of realizing above, the utility model provides a accurate trace water sample collection test piece for ATP fluorescence detects not only can gather trace liquid to liquid acquisition volume has high accuracy and uniformity.
The technical solution of the utility model is to provide a accurate trace water sample collection test element for ATP fluorescence detection, its special character lies in: comprises a spring cap, a puncture needle, a connector, a sampling rod and a reaction tube;
the spring cap comprises a reagent cavity and a spring structure, the spring structure is coaxially and fixedly connected with the reagent cavity, and the top end of the reagent cavity is communicated with the inside of the spring structure;
the puncture needle sleeve is arranged inside the spring cap, and the head of the puncture needle is contacted with the top of the spring structure; the tail part of the puncture needle is contacted with the connector;
the connecting body comprises a connecting part, a siphon pipe sleeved in the connecting part and a protective film sealed on one end face of the connecting part; one end of the connecting part, which is provided with a protective film, is hermetically connected with the bottom end of the reagent cavity, and the other end of the connecting part is hermetically connected with the opening end of the reaction tube;
the tail part of the puncture needle is contacted with the protective film; and the tail part of the puncture needle is coaxial with the siphon;
the sampling rod is positioned in the reaction tube, one end of the sampling rod is connected with the bottom of the siphon tube, and the other end of the sampling rod is a sampling end.
Furthermore, in order to realize accurate sampling, the sampling end sequentially comprises a transition section, a liquid collecting section and a liquid separating section from top to bottom;
the transition section is in a frustum shape, and the small end of the transition section is integrated with the sampling rod;
the liquid collecting section is provided with annular grooves along the circumferential direction of the outer wall of the sampling rod, and the annular grooves are coaxially arranged along the axial direction of the sampling rod;
the liquid separation section is conical, and the large end and the liquid collection section are integrally arranged.
Furthermore, an included angle is formed between two side walls of the annular groove and the two side walls are in smooth transition connection through a first cambered surface; and two adjacent grooves are in smooth transition connection through a second cambered surface.
Further, the side wall of one end, provided with the protective film, of the connecting part is in threaded sealing connection with the side wall of the bottom end of the reagent cavity, and the side wall of the other end of the connecting part is in threaded sealing connection with the side wall of the opening end of the reaction tube.
Further, the sampling tester also comprises a protective cap, and the protective cap is sleeved outside the spring cap.
Furthermore, the connecting part is in threaded connection with the reagent cavity and the reaction tube.
Furthermore, the outer wall of the middle part of the puncture needle is tightly attached to the inner wall of the spring cap.
The utility model has the advantages that:
the utility model provides a accurate trace water sample collection test element for ATP fluorescence detection, convenient operation can gather trace liquid, and liquid acquisition accuracy is high to the uniformity is high. This is crucial for the accuracy of the ATP fluorescence detection result of the liquid sample.
Drawings
FIG. 1 is a schematic view of the sampling probe of the present invention in a closed tube state;
FIG. 2 is a perspective view of the sampling device of the present invention in a closed state;
FIG. 3 is an exploded perspective view of the sampling device of the present invention in a closed tube state;
FIG. 4 is a side view of the sampling end of the sampling wand of the present invention;
FIG. 5 is a detailed identification drawing of a side view of a sampling end of a sampling wand in accordance with the present invention;
the reference numbers in the figures are:
1-protective cap, 2-spring cap, 21-spring structure, 22-reagent cavity, 3-puncture needle, 31-head, 32-middle, 33-tail, 4-protective film, 5-connector, 51-siphon, 52-connector, 6-sampling rod, 61-transition section, 62-liquid collection section, 63-liquid separation section, 64-annular groove, 65-first side wall, 66-second side wall, 7-reaction tube, and 8-sampling step.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
As can be seen from FIG. 1, the whole shape of the precise micro water sample collection reagent for ATP fluorescence detection in this embodiment is a rod shape, and includes a protective cap 1 and a collection reagent body. As can be seen from fig. 2 and 3, the collecting test element body of the present embodiment mainly includes a spring cap 2, a puncture needle 3, a connector 5, a sampling rod 6 and a reaction tube 7, wherein the spring cap 2, the connector 5 and the reaction tube 7 are sequentially connected from top to bottom. The protective cap 1 is sleeved outside the spring cap 2, so that the product is prevented from being damaged in the transportation process, and the phenomenon that the protective film is punctured by a puncture needle to cause reagent failure is prevented when the spring cap is pressed in the transportation process.
Wherein, spring cap 2 includes reagent cavity 22 and spring structure 21, and spring structure 21 links firmly with reagent cavity 22, and the inside intercommunication of reagent cavity 22 top and spring structure 21. In this embodiment, the reagent chamber 22 is a cylinder, the spring structure 21 is fixed at the upper end of the cylinder, and the telescopic direction of the spring structure is consistent with the axial direction of the cylinder. The reagent chamber 22 stores a reagent, and the spring structure 21 can provide a space compression required when the lancet 3 is pressed down and improve the comfort when the lancet 3 is pressed down by a user.
With reference to fig. 3, for convenience of description, the puncturing needle may be divided into three parts, which are a head part 31, a middle part 32 and a tail part 33 from top to bottom, wherein the diameter of the middle part 32 is between the tail part 33 and the middle part 32. In the unused state of the test strip, the puncture needle 3 is sleeved in the spring cap 2, and the head 31 penetrates through the top end of the reagent cavity 22 and penetrates through the center of the spring structure 21 to be in contact with the top of the spring structure 21; the outer wall of the middle part 32 is tightly attached to the inner wall of the reagent cavity 22 to prevent the puncture needle from shaking in the radial direction; the tail 33 abuts the protective membrane 4 of the connector 5.
The connecting body 5 is used for connecting the spring cap 2 and the reaction tube 7, and includes a connecting portion 52, a siphon 51 located inside the connecting portion 52, and a protective film 4 sealed on one end surface of the connecting portion. The side wall of the connecting portion 52 at the end provided with the protective film 4 is connected with the side wall of the lower end of the reagent chamber 22 in a screw-sealing manner, so that the tail portion 33 of the puncture needle 3 is in contact with the protective film 4. The other end side wall of the connecting part 52 is hermetically connected with the side wall of the opening end of the reaction tube 7 through screw threads. The siphon 51 inside the connector 5 has one end in contact with the protective film 4 and the other end connected to the sampling rod 6. The tail 33 of the puncture needle 3, the siphon 51 and the sampling rod 6 are coaxial, so as to ensure that a user presses the puncture needle 3 to puncture the protective film 4, and the reagent in the reagent cavity 22 passes through the siphon 51 of the connecting body 5 and is smoothly injected into the reaction tube along the sampling rod 6.
The utility model discloses material to the sampling stick is modified for the sampling stick surface keeps unified hydrophilicity. The specific structure of the sampling end of the sampling rod is shown in FIG. 4, and FIG. 4 is a side view of the sampling end; the sampling end is divided into three sections, namely a transition section 61, a liquid collection section 62 and a liquid separation section 63 from top to bottom;
wherein the transition section 61 is in a frustum shape, and the small end and the sampling rod are integrally arranged;
wherein the liquid collecting section 62 has the following specific structure: a plurality of annular grooves 64 are formed in the circumferential direction of the outer wall of the sampling rod, included angles are formed between two side walls (a first side wall 65 and a second side wall 66) of each annular groove 64, and the bottoms of the grooves are in smooth transition through first arc sections; the plurality of annular grooves are axially distributed along the sampling rod to form a multi-stage sampling step; and two adjacent grooves are in smooth transition connection through a second arc section.
The liquid separation section 63 is conical;
the section of the groove is cut along the axial direction of the sampling rod, and the section of the groove is shown in figure 5:
the depth of the groove determines the amount of water to be taken, in the embodiment, the diameter 1 is 2.85-3.05mm, and the diameter 2 is 5.95-6.25 mm.
The curvature radiuses R1 and R2 of the second arc segment and an included angle A1 between two side walls of the groove determine the shape of the sampled liquid, and the fullness degree of water taking is ensured, in the embodiment, the R1 and the R2 are 0.2-0.4mm, and the A1 is 28-30 degrees.
The curvature radius R3 of the first arc segment and the included angle A2 between the first side wall 65 of the groove and the central axis of the sampling rod together determine the bubble condition in the water taking process, the curvature radius R3 of the first arc segment in the embodiment takes a value of 0.2-0.4mm, and the curvature radius A2 takes a value of 88-91 degrees.
The taper angle A3 ensures that the sampling stick separates from the liquid when it is running out of the water, and does not stick to the liquid, in this example A3 is 58-62 deg..
The sampling rod comprises seven levels of sampling steps, and the sampling (double distilled water) precision of 100 +/-5 uL can be ensured through setting the parameters.
The use steps are as follows:
1. pulling out one end of the connector connected with the reaction tube;
2. placing a sampling rod in the liquid, and sampling the liquid sample by the sampling rod;
3. inserting the sampling rod back into the reaction tube;
4. removing the protective cap;
5. and pressing the spring cap to force the puncture needle to puncture the protective film, so that the reagent in the spring cap flows into the reaction tube along the sampling rod through the siphon pipe of the connecting body.
6. And (4) putting the test particles into an ATP fluorescence detector to finish the sampling test.
It is to be understood that the terms of orientation or positional relationship, such as "front, back, up, down, left, right", "transverse, vertical, horizontal" and "top, bottom", etc., are generally intended to refer to those illustrated in the drawings, merely for convenience and simplicity of description, and are not intended to limit the scope of the present invention in any way, since they do not indicate or imply that the referenced device or element must have a particular orientation or be constructed and operated in a particular orientation; the terms "inside" and "outside" refer to the inner and outer parts of the profile of the respective component itself, and the terms have no special meaning unless otherwise stated, and therefore should not be construed as limiting the scope of the present invention. In addition, it should be noted that fig. 1 to 5 are only examples, which are not drawn to scale, and should not be taken as limiting the scope of the invention as actually claimed.
Claims (7)
1. A accurate trace water sample collection test element for ATP fluorescence detection is characterized in that: comprises a spring cap (2), a puncture needle (3), a connector (5), a sampling rod (6) and a reaction tube (7);
the spring cap (2) comprises a reagent cavity (22) and a spring structure (21), the spring structure (21) is coaxially and fixedly connected with the reagent cavity (22), and the top end of the reagent cavity (22) is communicated with the inside of the spring structure;
the puncture needle (3) is sleeved inside the spring cap (2), and the head (31) of the puncture needle (3) is in contact with the top of the spring structure (21); the tail (33) of the puncture needle is contacted with the connector (5);
the connecting body (5) comprises a connecting part (52), a siphon (51) sleeved in the connecting part (52) and a protective film (4) sealed on one end face of the connecting part; one end of the connecting part (52) provided with a protective film is hermetically connected with the bottom end of the reagent cavity (22), and the other end of the connecting part (52) is hermetically connected with the opening end of the reaction tube (7);
the tail (33) of the puncture needle is in contact with the protective film; and the tail part (33) of the puncture needle (3) is coaxial with the siphon (51);
the sampling rod (6) is positioned in the reaction tube (7), one end of the sampling rod (6) is connected with the bottom of the siphon tube (51), and the other end of the sampling rod (6) is a sampling end.
2. The precise micro water sampling reagent for ATP fluorescence detection according to claim 1, wherein: the sampling end is provided with a transition section (61), a liquid collecting section (62) and a liquid separating section (63) from top to bottom in sequence;
the transition section (61) is in a frustum shape, and the small end and the sampling rod are integrally arranged;
the liquid collecting section (62) is provided with annular grooves along the circumferential direction of the outer wall of the sampling rod, and the annular grooves are coaxially arranged along the axial direction of the sampling rod;
the liquid separation section (63) is conical, and the large end of the liquid separation section is integrated with the liquid collection section (62).
3. The precise micro water sampling reagent for ATP fluorescence detection according to claim 2, wherein: an included angle is formed between two side walls of the annular groove and the two side walls are in smooth transition connection through a first cambered surface; and two adjacent grooves are in smooth transition connection through a second cambered surface.
4. The precise micro water sampling reagent for ATP fluorescence detection according to any one of claims 1-3, wherein: the side wall of one end, provided with the protective film, of the connecting part (52) is in threaded sealing connection with the side wall of the bottom end of the reagent cavity (22), and the side wall of the other end of the connecting part (52) is in threaded sealing connection with the side wall of the opening end of the reaction tube (7).
5. The precise micro water sampling reagent for ATP fluorescence detection according to claim 4, wherein: still include protective cap (1), protective cap (1) suit is in the outside of spring cap (2).
6. The precise micro water sampling reagent for ATP fluorescence detection according to claim 5, wherein: the connecting part is in threaded connection with the reagent cavity and the reaction tube.
7. The precise micro water sampling reagent for ATP fluorescence detection according to claim 6, wherein: the outer wall of the middle part (32) of the puncture needle is tightly attached to the inner wall of the spring cap.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922229682.4U CN211347519U (en) | 2019-12-13 | 2019-12-13 | Precise trace water sample collection test element for ATP fluorescence detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922229682.4U CN211347519U (en) | 2019-12-13 | 2019-12-13 | Precise trace water sample collection test element for ATP fluorescence detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211347519U true CN211347519U (en) | 2020-08-25 |
Family
ID=72099085
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922229682.4U Active CN211347519U (en) | 2019-12-13 | 2019-12-13 | Precise trace water sample collection test element for ATP fluorescence detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211347519U (en) |
-
2019
- 2019-12-13 CN CN201922229682.4U patent/CN211347519U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20200214611A1 (en) | Device for trapping an initial flow of blood | |
| US9642569B2 (en) | Syringe with removable plunger for arterial blood gas sample collection | |
| CN201476980U (en) | Device for sampling and testing | |
| EP2768394B1 (en) | Blood collection assembly | |
| CN211347519U (en) | Precise trace water sample collection test element for ATP fluorescence detection | |
| CN106308853A (en) | Bone marrow aspiration needle of hematology department | |
| US20050267384A1 (en) | Blood collection kit adapter | |
| CN202903768U (en) | Improved structure of quantitative sampling reagent feed device | |
| CN201179062Y (en) | Medical blood sampling equipment | |
| CN208228980U (en) | A kind of novel seal lumbar puncture needle assembly | |
| CN200939145Y (en) | Integrated sampling tube | |
| CN211094163U (en) | Animal blood collector | |
| CN220171051U (en) | Disease index detection device | |
| CN212539793U (en) | Constant-pressure sampling needle and sampling device | |
| CN201814581U (en) | Blood sampler for inspection | |
| CN215227692U (en) | Blood sampling assembly for blood gas analysis | |
| CN201668712U (en) | Test tube for blood test | |
| CN210077714U (en) | Saliva collection device | |
| CN210077713U (en) | Saliva collection system convenient to disassemble | |
| CN213345662U (en) | Finger blood collection and storage device for blood morphology examination and PCR detection | |
| CN215640364U (en) | Clinical laboratory uses sampler | |
| CN202761286U (en) | Visible blood return pen type blood taking needle | |
| CN214856735U (en) | Animal tail root trace blood sampler | |
| CN220109737U (en) | Neonate vacuum blood sampling tube | |
| CN212755662U (en) | Blood collection system that medical science inspection was used |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |