CN211325813U - Instrument group of sore is pressed in big mouse and little mouse of diabetes manufacturing - Google Patents
Instrument group of sore is pressed in big mouse and little mouse of diabetes manufacturing Download PDFInfo
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- CN211325813U CN211325813U CN201921990946.1U CN201921990946U CN211325813U CN 211325813 U CN211325813 U CN 211325813U CN 201921990946 U CN201921990946 U CN 201921990946U CN 211325813 U CN211325813 U CN 211325813U
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Abstract
The utility model relates to a tool set for manufacturing pressure sores for diabetic rats and mice, which comprises a holding rod, a puncture rod, a diamond puncture needle head, a push button, a separation blade and a magnetic indentation device; the front end of the holding rod is fixedly connected with a puncture rod; a T-shaped groove is formed in the puncture rod, a diamond puncture needle head is fixedly connected to the front end of the puncture rod, a push button is installed in the T-shaped groove, and two separation blades are fixedly connected to the front of the push button; a separation pen placing groove is formed in the upper surface of the magnetic indentation device, a circular groove is formed in the side surface of the magnetic indentation device, and a wound manufacturing groove is formed in the lower surface of the magnetic indentation device. Its advantages are: simplifying the original experimental steps, improving the experimental technology and standardizing the experimental operation; the area and the size of the wound can be freely controlled; the wound of the operation part is small, the operation time is short, and the infection risk in the operation of the experimental mouse is small; the secondary operation is not needed, the magnetic sheet is not needed to be implanted, the wound to the experimental mouse is reduced, the experimental mouse is in an anesthesia state in the experimental process, and the discomfort of the experimental mouse in the experimental operation is reduced.
Description
Technical Field
The utility model belongs to the technical field of the experimental apparatus technique and specifically relates to a diabetes big mouse makes instrument group who presses sore.
Background
Diabetes belongs to a chronic metabolic disease, and one of the complications of diabetes patients is poor wound healing.
The wounds of the diabetics are generally healed slowly, the wounds are difficult to heal due to the fact that the wounds are often combined with microbial infection clinically, amputation can be caused when the infection process is serious, the disability and fatality rate of the diabetics is increased, certain economic burden is caused to the diabetics and families of the diabetics, and the diabetic complication is one of the problems to be solved urgently at present.
In recent years, the research on the healing of diabetic wounds is increasing at home and abroad, and the field has become a common research direction in a plurality of medical or biological fields such as a wound management center, orthopedics, burn department, endocrinology department, dermatology department and the like.
In the aspect of researching diabetic wound healing, a disease model animal, particularly a diabetes experimental mouse model is one of important tools for researching diabetic wound healing, and as a classic model animal for researching diabetic wound healing, the disease model animal not only can clarify a delayed healing mechanism of a diabetic wound from basic experiments, pathological detection and various biochemical indexes, but also can be used for verifying the effectiveness and safety of a new medicine and a new therapy.
At present, diabetic wound model animals for various researches in scientific research work mainly take experimental mice as main materials, and the animals comprise rats (SD), black mice (C57BL/6), white mice (Kunming mice) and the like. The method for preparing the diabetic wound animal model by using the experimental mouse mainly comprises the following steps:
1. acute and chronic wound models: when the model is made, the whole skin of the diabetic laboratory mouse is partially cut by a scalpel to form a round wound. The technical requirements of skin cutting by using a scalpel on surgical operators are high, the cutting area of the same group of experimental mice needs to have good uniformity, the requirement is difficult to achieve due to certain elasticity and ductility of the experimental mouse skin, the experimental mice are additionally damaged by improper operation, the molding success rate is reduced, and the experiment cost is increased; the skin biopsy punch is an experimental method improved on the basis of cutting by a scalpel, but has the disadvantages that the whole skin layer cannot be completely cut and separated, only a round indentation with uniform size can be formed on the skin of an experimental mouse through pressure, tools such as an ophthalmic scissors or a surgical scissors need to be used again to cut and separate the whole skin layer along the round indentation formed by the tools, although the difficulty of the surgical operation is reduced, the whole molding operation process is complicated, the cut edge of the ophthalmic scissors or the surgical scissors is often uneven, and subsequent shooting sample retention and data statistics can be influenced.
2. Infection wound model: on the basis of an acute or chronic wound model of a diabetic mouse, common pathogenic bacteria such as pseudomonas aeruginosa, staphylococcus aureus and the like are inoculated in a wound locally, so that the actual condition of a diabetic patient after clinical wound infection can be simulated.
3. Pressure sore or ischemia reperfusion wound model: preparing two magnetic sheets with proper size, and sterilizing for later use. Firstly, making an incision reaching fascia on the back of a diabetic laboratory mouse, and separating skin and subcutaneous fascia in a blunt manner; then implanting one of the magnetic sheets under the skin, and suturing the wound; finally, the in vitro magnetic sheet and the in vivo transplanted magnetic sheet mutually attract to generate pressure on the skin to cause local skin ischemia, and the in vitro magnetic sheet is removed after a certain period of ischemia to cause the animal model of the diabetic pressure sore or the local ischemia reperfusion wound.
Chinese patent documents: CN106264769A, published: 2017.01.04, discloses a preparation method and application of a rat local skin pressure sore model, which is mainly characterized in that the rat local skin pressure sore model is established by an ischemia-reperfusion circulation injury method; the condition of model establishment is evaluated by selecting rats for standby experiments, carrying out preparation work before the experiments, then weighing and anaesthetizing, and carrying out model preparation and detection of clinical indexes.
Chinese patent documents: CN203710182U, published: 2014.07.16, discloses an animal pressure sore model machine, comprising a base, a balance board arranged on the base, a beam arranged on the balance board, a pressure device arranged on the beam, a sensor and a pressure display; the pressure device applies force to the simulant; the sensor monitors the force applied by the pressure gauge and transmits it to the pressure display.
However, the tool set for producing pressure sores for diabetic rats and mice of the present invention has not been reported yet.
Disclosure of Invention
The utility model aims at providing a tool set for manufacturing pressure sores for diabetic rats and mice.
In order to achieve the purpose, the utility model adopts the technical proposal that:
a tool set for manufacturing pressure sores for diabetic rats and mice comprises a hand-held rod 1, a puncture rod 2, a diamond puncture needle head 3, a push button 4, a separation blade 5 and a magnetic indentation device 6; the holding rod 1 is in a cylindrical rod shape, and the front end of the holding rod 1 is fixedly connected with a puncture rod 2; a T-shaped groove 21 is formed in the puncture rod 2, a diamond-shaped puncture needle head 3 is fixedly connected to the front end of the puncture rod 2, and the diamond-shaped puncture needle head 3 is in a quadrangular pyramid shape; a push button 4 is arranged in the T-shaped groove 21, and a T-shaped pin 41 is arranged at the lower end of the push button 4; two separating blades 5 are fixedly connected in front of the push button 4; one end of the separation blade 5 is fixedly connected with the rhombic puncture needle head 3, and the other end of the separation blade is fixedly connected with the push button 4;
the magnetic indentation device 6 is integrally cylindrical, a separation pen placing groove 61 is formed in the upper surface of the magnetic indentation device 6, a circular groove 62 is formed in the side surface of the magnetic indentation device 6, and a wound manufacturing groove 63 is formed in the lower surface of the magnetic indentation device 6.
The T-shaped groove 21 is matched with the T-shaped pin 41.
The push button 4 can slide left and right in the T-shaped groove 21.
The separating blade 5 has certain toughness and can be attracted by a magnet.
The bottom of the wound making groove 63 has magnetism.
The utility model has the advantages that:
1. the utility model discloses a tool set for manufacturing pressure sores for diabetic rats and mice, which is specially used for establishing acute and chronic wound models of the diabetic rats and wound models of pressure sores or ischemia reperfusion laboratory rats; simplify the original experimental steps, improve the experimental technique and standardize the experimental operation.
2. The utility model discloses a diabetes big mouse makes instrument group of pressing sore can adjust wound area size through the distance that impels button regulation separation blade to extend, and the wound makes recess standard circular, makes wound area and size all can freely control.
3. The utility model discloses a diabetes rat and mouse make instrument group of pressing sore, the design of rhombus puncture syringe needle makes the operation position wound little, and operating time is short, and the infection risk is little in the laboratory mouse art.
4. The utility model discloses a diabetes rat and mouse make instrument group of pressing sore does not need the secondary operation, after the puncture, can use magnetic indentation device to press and cut complete epidermis downwards and cause the wound.
5. The utility model discloses a diabetes big and small mouse makes instrument group who presses sore does not need the magnetic sheet to implant, reduces the wound to the laboratory mouse, and simple to use is swift, and the laboratory mouse is in the anesthesia state in the experimentation, has reduced its uncomfortable sense in experimental operation.
Drawings
FIG. 1 is a schematic structural diagram of a tool set for producing pressure sores for diabetic rats and mice.
FIG. 2 is a schematic view of the combination of a puncture rod and a diamond puncture needle of a tool set for pressure sores production of diabetic rats and mice.
FIG. 3 is a schematic diagram of a push button structure of a tool set for producing pressure sores for diabetic rats and mice.
FIG. 4 is a schematic view of the combination of a puncture rod, a diamond puncture needle head and a push button of a tool set for producing pressure sores for diabetic rats and mice.
FIG. 5 is a first schematic diagram of the combination of a puncture rod, a diamond puncture needle, a push button and a separation blade of a tool set for producing pressure sores for diabetic rats and mice.
FIG. 6 is a second schematic view of the combination of the puncture rod, the diamond puncture needle, the push button and the separation blade of the pressure sore making tool set for diabetic rats and mice.
FIG. 7 is a schematic view of the combination of a holding rod, a puncturing rod, a diamond-shaped puncturing needle, a pushing button and a separating blade of a tool set for producing pressure sores for diabetic rats and mice.
FIG. 8 is a schematic structural view of a magnetic indentation device of a tool set for producing pressure sores for diabetic rats and mice.
FIG. 9 is a schematic diagram of the use of a kit for creating pressure sores in diabetic rats and mice.
Detailed Description
The invention will be further described with reference to the following examples and with reference to the accompanying drawings.
The reference numerals and components referred to in the drawings are as follows:
1. hand holding rod
2. Puncture rod
21. T-shaped groove
3. Diamond puncture needle head
4. Push button
41. T-shaped pin
5. Separating blade
6. Magnetic indentation device
61. Separation pen placing groove
62. Circular groove
63. Wound manufacturing groove
Example 1
Referring to fig. 1, fig. 1 is a schematic structural diagram of a tool set for producing pressure sores for diabetic rats and mice according to the embodiment. The tool set for manufacturing pressure sores for the diabetic rats and the diabetic mice comprises a holding rod 1, a puncture rod 2, a diamond puncture needle head 3, a push button 4, a separation blade 5 and a magnetic indentation device 6;
referring to fig. 2-7, fig. 2 is a schematic diagram of a combination of a puncture rod and a diamond puncture needle of a tool set for manufacturing pressure sores for diabetic rats and mice in this embodiment, fig. 3 is a schematic diagram of a structure of a push button of a tool set for manufacturing pressure sores for diabetic rats and mice in this embodiment, fig. 4 is a schematic diagram of a combination of a puncture rod, a diamond puncture needle and a push button of a tool set for manufacturing pressure sores for diabetic rats and mice in this embodiment, fig. 5 is a schematic diagram of a combination of a puncture rod, a diamond puncture needle, a push button and a separation blade of a tool set for manufacturing pressure sores for diabetic rats and mice in this embodiment, fig. 6 is a schematic diagram of a combination of a puncture rod, a diamond puncture needle, a push button and a separation blade of a tool set for manufacturing pressure sores for diabetic rats and mice in this embodiment, fig. 7 is a schematic diagram of a combination of a grip lever, a diamond puncture rod, a diamond puncture needle, a push button and, The puncture rod, the diamond puncture needle head, the push button and the separation blade are combined. The holding rod 1 is in a cylindrical rod shape, and the front end of the holding rod 1 is fixedly connected with a puncture rod 2; a T-shaped groove 21 is formed in the puncture rod 2, a diamond-shaped puncture needle head 3 is fixedly connected to the front end of the puncture rod 2, and the diamond-shaped puncture needle head 3 is in a quadrangular pyramid shape; a push button 4 is arranged in the T-shaped groove 21, and a T-shaped pin 41 is arranged at the lower end of the push button 4; two separating blades 5 are fixedly connected in front of the push button 4; one end of the separation blade 5 is fixedly connected with the rhombic puncture needle head 3, and the other end of the separation blade is fixedly connected with the push button 4;
referring to fig. 8, fig. 8 is a schematic structural view of a magnetic indentation apparatus of a tool set for manufacturing pressure sores for diabetic rats and mice according to the present embodiment. The magnetic indentation device 6 is integrally cylindrical, a separation pen placing groove 61 is formed in the upper surface of the magnetic indentation device 6, a circular groove 62 is formed in the side surface of the magnetic indentation device 6, and a wound manufacturing groove 63 is formed in the lower surface of the magnetic indentation device 6.
The T-shaped groove 21 is matched with the T-shaped pin 41.
The push button 4 can slide left and right in the T-shaped groove 21.
The separating blade 5 has certain toughness and can be attracted by a magnet.
The bottom of the wound making groove 63 has magnetism.
Example 2
Referring to fig. 1 and 9, fig. 1 is a schematic structural diagram of a tool set for manufacturing pressure sores for diabetic rats and mice in this embodiment, and fig. 9 is a schematic use diagram of the tool set for manufacturing pressure sores for diabetic rats and mice in this embodiment.
The use process and the method of the tool set for manufacturing the pressure sores of the diabetic rats and the diabetic rats comprise the following steps:
firstly anaesthetizing the rat/mouse, removing the back hair of the rat/mouse, disinfecting with iodine, and deiodinating with alcohol to finish skin preparation; selecting a proper needle insertion point on the back of the rat \ mouse for puncture; after the penetration into the subcutaneous tissue, the diamond-shaped puncture needle head 3 and the puncture rod 2 enter a certain depth, and the push button 4 is pushed; → the two separation blades 5 connected in front of the push button 4 will naturally expand to blunt the subcutaneous tissue, resulting in a pocket-like cavity; the separating blade 5 after the dilatation forms a quasi-circular shape, at the moment, a groove 63 is made outside the back skin for inosculating the wound, and simultaneously, the puncture rod 2 is lifted upwards to be cut or pressed evenly, and finally, the diabetic rat/mouse wound or pressure sore is formed.
Example 3
The method for establishing the acute and chronic wound models of the diabetes experimental mice comprises the following steps:
1. anesthesia: mice (SD rat/KM mouse/C57 BL/6 mouse) were anesthetized by intraperitoneal injection with 4% chloral hydrate (100. mu.L/10 g). The body turning reflex of the experimental mouse disappears, and the hind limb of the experimental mouse is pinched without retraction, so that the anesthesia is successful and the next step can be carried out.
2. Skin preparation: selecting skin of back or flank, removing hair, sterilizing with iodine tincture, and removing iodine with 75% alcohol to obtain skin preparation.
3. Puncturing: the skin of the back or the flank of the experimental mouse is lifted by forceps, a puncture point is selected, and the diamond puncture needle head 3 is used for puncturing the epidermis and entering the subcutaneous part. The diamond-shaped puncture needle head 3 can freely pass through the subcutaneous part, and the puncture is considered to be successful.
4. Blunt separation: after the diamond puncture needle head 3 and the puncture rod 2 enter the subcutaneous space, the push button 4 is slid to ensure that the two separation blades 5 behind the diamond puncture needle head 3 slide out of the puncture rod 2, and the separation blades 5 naturally expand after entering the subcutaneous space, so that the subcutaneous tissue can be separated in a blunt way to form a pocket-shaped subcutaneous cavity. The sliding distance of the push button 4 is adjusted to adjust the area of the desired wound.
5. Wound creation: the magnetic indentation device 6 presses down to make the wound manufacturing groove 63 adhere to the opened separation blade 5, the separation blade 5 is automatically adsorbed in the wound manufacturing groove 63 due to the magnetic force at the bottom of the wound manufacturing groove 63, and the magnetic indentation device 6 is slightly twisted to make the annular blade in the wound manufacturing groove 63 contact with the skin and cut uniformly.
6. Debridement: and taking down the magnetic indentation device 6, drawing out the diamond puncture needle head 3 and the puncture rod 2, and cleaning the wound by using normal saline. The experimental mice were placed in a warm environment until they were revived.
Example 4
The method for establishing the model of the pressure sore or ischemia-reperfusion wound of the diabetic laboratory mouse comprises the following steps:
1. anesthesia: mice (SD rat/KM mouse/C57 BL/6 mouse) were anesthetized by intraperitoneal injection with 4% chloral hydrate (100. mu.L/10 g). The body turning reflex of the experimental mouse disappears, and the hind limb of the experimental mouse is pinched without retraction, so that the anesthesia is successful and the next step can be carried out.
2. Skin preparation: selecting skin of back or flank, removing hair, sterilizing with iodine tincture, and removing iodine with 75% alcohol to obtain skin preparation.
3. Puncturing: the skin of the back or the flank of the experimental mouse is lifted by forceps, a puncture point is selected, and the diamond puncture needle head 3 is used for puncturing the epidermis and entering the subcutaneous part. The diamond-shaped puncture needle head 3 can freely pass through the subcutaneous part, and the puncture is considered to be successful.
4. Blunt separation: after the diamond puncture needle head 3 and the puncture rod 2 enter the subcutaneous space, the push button 4 is slid to ensure that the two separation blades 5 behind the diamond puncture needle head 3 slide out of the puncture rod 2, and the separation blades 5 naturally expand after entering the subcutaneous space, so that the subcutaneous tissue can be separated in a blunt way to form a pocket-shaped subcutaneous cavity. The sliding distance of the push button 4 is adjusted to adjust the area of the desired wound.
5. Wound creation: the open separation blade 5 of laminating is pressed down with magnetic indentation device 6, and separation blade 5 can adsorb in wound makes recess 63 automatically because of magnetic force, does not need to press down magnetic indentation device 6 hard to keep oppressing 40min, rest 20min, repeat 6 times, observe the skin ischemia condition after oppressing at every turn, treat that extravasated blood appears in the skin, the skin colour turns to the dark and can remove magnetic indentation device 6, rhombus puncture syringe needle 3 and puncture pole 2.
6. Debridement: and taking down the magnetic indentation device 6, drawing out the diamond puncture needle head 3 and the puncture rod 2, and cleaning the wound by using normal saline. The experimental mice were placed in a warm environment until they were revived.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and additions can be made without departing from the principles of the present invention, and these improvements and additions should also be regarded as the protection scope of the present invention.
Claims (5)
1. A tool set for manufacturing pressure sores for diabetic rats and mice is characterized by comprising a hand-held rod (1), a puncture rod (2), a diamond puncture needle head (3), a push button (4), a separation blade (5) and a magnetic indentation device (6); the hand-held rod (1) is in a cylindrical rod shape, and the front end of the hand-held rod (1) is fixedly connected with a puncture rod (2); a T-shaped groove (21) is formed in the puncture rod (2), a diamond-shaped puncture needle head (3) is fixedly connected to the front end of the puncture rod (2), and the diamond-shaped puncture needle head (3) is in a quadrangular pyramid shape; a push button (4) is arranged in the T-shaped groove (21), and a T-shaped pin (41) is arranged at the lower end of the push button (4); two separating blades (5) are fixedly connected in front of the push button (4); one end of the separation blade (5) is fixedly connected with the rhombic puncture needle head (3), and the other end of the separation blade is fixedly connected with the pushing button (4);
the magnetic indentation device (6) is integrally cylindrical, a separation pen placing groove (61) is formed in the upper surface of the magnetic indentation device (6), a circular groove (62) is formed in the side surface of the magnetic indentation device (6), and a wound manufacturing groove (63) is formed in the lower surface of the magnetic indentation device (6).
2. The kit for creating pressure sores of diabetic rats and mice as recited in claim 1, wherein the T-shaped groove (21) is engaged with the T-shaped pin (41).
3. The kit for making pressure sores for diabetic rats and mice as claimed in claim 1, wherein the push button (4) is slidable left and right in the T-shaped slot (21).
4. The kit for making pressure sores for diabetic rats and mice as claimed in claim 1, wherein the separating blade (5) has a certain toughness and can be attracted by a magnet.
5. The diabetic rat and mouse pressure sore making kit according to claim 1, wherein the bottom of the wound making groove (63) is magnetic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921990946.1U CN211325813U (en) | 2019-11-18 | 2019-11-18 | Instrument group of sore is pressed in big mouse and little mouse of diabetes manufacturing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921990946.1U CN211325813U (en) | 2019-11-18 | 2019-11-18 | Instrument group of sore is pressed in big mouse and little mouse of diabetes manufacturing |
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| Publication Number | Publication Date |
|---|---|
| CN211325813U true CN211325813U (en) | 2020-08-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921990946.1U Active CN211325813U (en) | 2019-11-18 | 2019-11-18 | Instrument group of sore is pressed in big mouse and little mouse of diabetes manufacturing |
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| Country | Link |
|---|---|
| CN (1) | CN211325813U (en) |
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- 2019-11-18 CN CN201921990946.1U patent/CN211325813U/en active Active
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