CN211292934U - Colloidal gold test strip for detecting pesticide and veterinary drug and illegal additives - Google Patents
Colloidal gold test strip for detecting pesticide and veterinary drug and illegal additives Download PDFInfo
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- CN211292934U CN211292934U CN201922485723.6U CN201922485723U CN211292934U CN 211292934 U CN211292934 U CN 211292934U CN 201922485723 U CN201922485723 U CN 201922485723U CN 211292934 U CN211292934 U CN 211292934U
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Abstract
The utility model relates to a biological detection field, in particular to colloidal gold test paper strip for detecting veterinary drugs and illegal additives. The test strip comprises a bottom plate and a sample pad, a colloidal gold combination pad, a buffer pad, a reaction membrane, a water absorption pad and a non-setting adhesive which are sequentially and tightly connected on the bottom plate; the colloidal gold bonding pad is coated with a labeled antibody which can be combined with the veterinary drug and the illegal additive; the buffer pad is coated with buffer solution; an amino acid line, a detection line and a quality control line are sequentially arranged on the reaction membrane along the chromatography direction; the amino acid coil is coated with an amino acid solution; the detection line is coated with an artificial synthetic antigen, and the quality control line is coated with a goat anti-mouse IgG secondary antibody which can be combined with the labeled antibody. The test strip can be quickly used for detecting veterinary drugs and illegal additives, has high sensitivity, uniform color development, good detection result accuracy and high precision, and effectively solves the problems of low sensitivity, uneven color development and insufficient accuracy of the existing products.
Description
Technical Field
The utility model relates to a biological detection field, in particular to colloidal gold test paper strip for detecting veterinary drugs and illegal additives.
Background
The colloidal gold immunochromatography reagent plate has the characteristics of single detection, simple and quick operation, good specificity, high sensitivity, no need of auxiliary reagents and instruments, visual observation results, long validity period and the like, is one of the fastest, sensitive and convenient immunological detection technologies at present, and is particularly suitable for field operation, time-critical inspection and large-area general investigation.
In the conventional common reagent plate device for rapidly detecting the immune colloidal gold, when a sample solution is dripped on a sample pad, a substance to be detected in the sample solution and a gold-labeled antibody on a gold-labeled antibody binding pad perform a specific binding reaction between antigens and antibodies, the solution to be detected quickly migrates to the other end of a membrane strip along with the capillary action of a nitrocellulose membrane, the gold-labeled antibody which is not bound with the substance to be detected in the sample solution performs the specific binding reaction between the antigens and the antibodies with the specific binding antigen on a detection line, and the remaining gold-labeled antibody is bound with a second antibody (generally goat anti mouse IgG) on a control line, so that the detection line and the control line show color changes.
In the reaction process, if the reaction between the substance to be detected in the sample solution and the gold-labeled antibody on the gold-labeled antibody binding pad is insufficient, the residual unbound gold-labeled antibody is relatively increased, so that the amount of the gold-labeled antibody bound with the specific binding antigen on the detection line is increased, the detection line develops color and becomes dark, according to the detection result interpretation mode of the immune colloidal gold rapid detection reagent plate, the color depth difference between the detection line and the control line is reduced, the detection line possibly develops color deeper or the same depth as the control line, so that the detection result is changed from positive to negative, and the detection sensitivity of the product is reduced. Therefore, whether the reaction between the substance to be detected in the sample solution and the gold-labeled antibody on the gold-labeled antibody binding pad is sufficient or not is determined to a great extent, and the sensitivity of the immune colloidal gold rapid detection reagent plate is determined. Factors influencing the sufficiency of the reaction between the substance to be detected in the sample solution and the gold-labeled antibody on the gold-labeled antibody binding pad mainly include a nitrocellulose membrane (NC membrane), reaction time and stability of a reaction system, in addition to the self-affinity factors of the antigen and the antibody. The NC membrane has different pore diameters, hydrophobic and hydrophilic properties and the like, which can generate difference on the sensitivity of the same immune colloidal gold rapid detection reagent plate. At present, cellulose nitrate membranes with good stability and small batch to batch differences are supplied by Millipore (USA), Whatman and S & S, Sartorius (Germany), Yineng (domestic), MDI (India). More commonly used are Millipore, Whatman and Sartorius. After a plurality of tests, the NC membrane manufacturers screen several membranes with the best universality to supply markets on the basis of a large amount of experimental data. Therefore, the research space for improving the sensitivity of the immune colloidal gold rapid detection reagent plate by researching different NC membranes is small. The more stable and sufficient the reaction system of the analyte in the sample solution and the gold-labeled antibody on the gold-labeled antibody conjugate pad, the more sufficient the reaction between the analyte and the gold-labeled antibody. In the conventional immune colloidal gold rapid detection reagent plate device, the reaction time of an object to be detected in a sample solution and a gold-labeled antibody on a gold-labeled antibody binding pad is short, so that the reaction between the object to be detected and the gold-labeled antibody is insufficient, and the sensitivity is affected.
In addition, the phenomenon of uneven color development of the detection line (T line) may occur during the detection of the traditional colloidal gold test strip, for example, the color of the lower end of the line is dark, the color of the upper end of the line is light, or the color of the left end and the right end of the line is uneven, and the like, which is mainly due to the influence of various factors such as raw materials, nitrocellulose membranes, membrane dispensing machines and the like. After the conditions are met, the optimization can be carried out by replacing raw materials (antigens and antibodies), screening and replacing the nitrocellulose membrane, screening the stabilizer, a buffer system and the like. The screening of antigens and antibodies with good quality has certain possibility of improving the line aiming at a certain product, but cannot improve the line condition of all products, and has no universality. The screening work of the stabilizer and the buffer system is complicated and complicated, and different products are influenced by the stabilizer and the buffer system to different degrees, so the practical operation feasibility is not high.
Therefore, the development of the colloidal gold test strip which has uniform color development, high precision and high sensitivity and is generally suitable for detecting the pesticide and veterinary drugs and illegal additives has important significance.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a detect colloidal gold test paper strip of animal remedy and illegal additive. The colloidal gold test strip provided by the utility model has clear and even color development and higher precision, and is generally suitable for detection of veterinary drugs and illegal additives.
The utility model provides a colloidal gold test strip for detecting veterinary drugs and illegal additives, which comprises a bottom plate and a sample pad, a colloidal gold combination pad, a buffer pad, a reaction membrane, a water absorption pad and an adhesive sticker which are sequentially and tightly connected on the bottom plate;
the colloidal gold bonding pad is coated with a labeled antibody which is combined with the veterinary drug and illegal additives; the buffer pad is coated with buffer solution; an amino acid line, a detection line and a quality control line are sequentially arranged on the reaction membrane along the chromatography direction; the amino acid coil is coated with an amino acid solution; the detection line is coated with an artificially synthesized antigen; and the quality control line is coated with a goat anti-mouse IgG secondary antibody which can be combined with the labeled antibody.
The utility model provides a colloidal gold test paper strip has an amino acid line at the one end peridium that the reaction film is close to the colloidal gold and combines the pad, and the experiment shows, and the colloidal gold test paper strip color development that increases the amino acid line is even, and precision and accuracy are all higher, has obviously improved current colloidal gold test paper strip T line color development inhomogeneous to and the lower problem of quantitative determination result accuracy that leads to by the color development inhomogeneous.
In some embodiments, the amino acid solution consists of leucine, phenylalanine, threonine, lysine, aspartic acid, and a preservative in PBS buffer.
In some embodiments, the amino acid solution is 0.01M PBS buffer pH7.2-7.4 containing 0.05-0.2% leucine, 0.05-0.2% phenylalanine, 0.05-0.2% threonine, 0.05-0.2% lysine, 0.05-0.2% aspartic acid, and 0.1-0.3% sodium azide.
In some embodiments, the amino acid solution is 0.1% leucine, 0.1% phenylalanine, 0.1% threonine, 0.1% lysine, 0.1% aspartic acid and 0.2% sodium azide in 0.01M PBS buffer, ph 7.4.
In some embodiments, the buffer solution coated on the buffer pad is 0.1mol/L phosphate buffer solution containing 1% of polyethylene glycol octyl phenyl ether and 1% of bovine serum albumin.
In the utility model, the colloidal gold bonding pad is coated with a labeled antibody which is combined with the antigen. In some embodiments, the labeled antibody is a colloidal gold labeled monoclonal antibody.
In the utility model, the artificially synthesized antigen is a conjugate of an animal pesticide, an illegal additive and a carrier protein.
In some embodiments, the veterinary drug and illegal supplement is aflatoxin B1, chloramphenicol, clenbuterol hydrochloride, or furazolidone metabolite.
In some embodiments, the carrier protein is one of bovine serum albumin, ovalbumin, hemocyanin.
In some embodiments, the amino acid line is 4-6 mm away from the detection line.
In some embodiments, the base plate is a PVC plate, the sample pad, the buffer pad and the colloidal gold conjugate pad are made of glass fiber, and the reaction membrane is an NC membrane.
The utility model discloses in the colloidal gold test paper strip, there is 1 ~ 2 mm's overlap between sample pad, colloidal gold combination pad, nitrocellulose membrane and the adjacent each part of pad that absorbs water. The purpose is to ensure that the chromatography is smoothly carried out from the sample pad to the water absorption pad, and to ensure that the sample solution has sufficient reaction time from the sample pad to the reaction membrane through the gold-labeled binding pad, so as to ensure that the effective components in the sample solution smoothly react with the gold-labeled antibody in the gold-labeled binding pad.
The utility model also provides a colloidal gold reagent board, include the utility model discloses colloidal gold test paper strip and the plastic casing 11 of parcel colloidal gold test paper strip are equipped with application of sample hole S and observation window 12 on the plastic casing 11. The plastic shell 11 has the functions of fixing the colloidal gold test strip and marking. When the test paper is used, a sample to be tested is dripped on the sample pad 1 of the colloidal gold test paper strip through the sample adding hole S, the coloration conditions of the C line and the T line are observed through the observation window 12 after standing for several minutes, the conditions of the pesticide and the illegal additive in the sample are qualitatively judged and detected according to an interpretation method, and the content of the pesticide and the illegal additive can be quantitatively detected by combining a colloidal gold reading instrument. When the micromolecule drug is drug, the colloidal gold test strip can qualitatively and quantitatively detect the drug residue in the sample to be detected.
The utility model discloses colloidal gold test paper strip is based on competition method principle, and the mark antibody on the artificial antigen competition combination colloidal gold conjugate pad of pesticide and illegal additive and reagent on the detection line in the sample, the color development degree on the detection line and the sample that awaits measuring in pesticide and illegal additive content inverse ratio.
The reading and interpretation method of the qualitative detection result comprises the following steps: when the sample does not contain the detected veterinary drug and illegal additives or the content of the detected veterinary drug and illegal additives is lower than the detection limit, the color development of the T line of the test strip is stronger than or equal to that of the C line, and the test strip is negative; the T line is positive without color development or weaker than the C line; the C line did not develop color, and the test strip was ineffective regardless of whether T developed color or not.
The colloidal gold test strip for detecting the veterinary drugs and illegal additives comprises a bottom plate and a sample pad, a colloidal gold combination pad, a buffer pad, a reaction membrane, a water absorption pad and an adhesive sticker which are sequentially and tightly connected on the bottom plate; the colloidal gold bonding pad is coated with a labeled antibody which is combined with the veterinary drug and illegal additives; the buffer pad is coated with buffer solution; an amino acid line, a detection line and a quality control line are sequentially arranged on the reaction membrane along the chromatography direction; the amino acid coil is coated with an amino acid solution; the detection line is coated with an artificially synthesized antigen; and the quality control line is coated with a goat anti-mouse IgG secondary antibody which can be combined with the labeled antibody. Compare with current colloidal gold test paper strip, the utility model discloses the test paper strip has advantages such as sensitivity is high, the color development is even, precision height, and uses easy and simple to handle, does not need the professional to operate.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic structural diagram and a top view of the colloidal gold test strip of the present invention;
wherein, 1 shows a sample pad, 2 shows a colloidal gold combined pad, 3 shows a buffer pad, 4 shows a reaction membrane, 5 shows an amino acid line, 6 shows a detection line, 7 shows a quality control line, 8 shows a water absorption pad, 9 shows a self-adhesive, and 10 shows a PVC bottom plate;
FIG. 2 is a schematic view of the operation of the colloidal gold reagent plate of the present invention; wherein, S shows a sample adding hole, 6 shows a detection line, 7 shows a quality control line, 11 shows a plastic shell, and 12 shows an observation window;
FIG. 3 is a schematic diagram of the result judgment of the colloidal gold test strip and the reagent plate of the present invention, wherein T is a detection line and C is a quality control line; the color development of the T line is deeper than that of the C line or the color development intensity is the same, and the T line is negative; the T line is not developed or is developed lighter than the C line and is positive; the C line did not develop color and was not effective;
FIG. 4 is a schematic structural diagram of a conventional colloidal gold test strip; wherein, 1 is a sample pad, 2 is a colloidal gold combined pad, 3 is a reaction membrane, 4 is a detection line, 5 is a quality control line, 6 is a water absorption pad, and 7 is a PVC bottom plate;
FIG. 5 is a comparison of the results of chloramphenicol detection using the conventional colloidal gold test strip and the colloidal gold test strip of the present invention, wherein 5-a-5-b are the results of detection with five amino acids at a concentration of 0.05%, 5-c-5-d are the results of detection with five amino acids at a concentration of 0.1%, and 5-e-5-f are the results of detection with five amino acids at a concentration of 0.2%;
FIG. 6 shows the influence of amino acid lines with different concentrations on the color development of aflatoxin B1 colloidal gold test strips, wherein 6-a is the detection result of the traditional colloidal gold test strips, and 6-B is the detection result of the colloidal gold test strips of the present invention;
FIG. 7 is a comparison of the results of the detection of clenbuterol hydrochloride by using a conventional colloidal gold test strip and the colloidal gold test strip of the present invention, wherein 7-a to 7-b are the results of the detection of the conventional colloidal gold test strip, and 7-c to 7-d are the results of the detection of the colloidal gold test strip of the present invention;
FIG. 8 is a comparison of the results of chloramphenicol detection using a conventional colloidal gold test strip and the colloidal gold test strip of the present invention, wherein 8-a to 8-b are the results of conventional colloidal gold test strips, and 8-c to 8-d are the results of the colloidal gold test strip of the present invention;
fig. 9 is a comparison of the detection results of the conventional colloidal gold test strip and the colloidal gold test strip of the present invention on furazolidone metabolites, wherein 9-a-9-b are the detection results of the conventional colloidal gold test strip, and 9-c-9-d are the detection results of the colloidal gold test strip of the present invention.
Detailed Description
The utility model discloses a colloidal gold test strip for detecting veterinary drugs and illegal additives, which can be realized by the technical personnel in the field by referring to the contents of the test paper and properly improving the technological parameters. It is expressly noted that all such substitutions and modifications which are obvious to a person skilled in the art are deemed to be included in the present invention. While the methods and applications of the present invention have been described in terms of preferred embodiments, it will be apparent to those skilled in the art that variations and modifications, or appropriate alterations and combinations, may be made to the methods and applications described herein without departing from the spirit and scope of the invention as defined by the appended claims.
The description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
The utility model provides an in the colloidal gold test paper strip of detection animal remedy and illegal additive, reagent, material, the instrument of adoption all are ordinary market article, all can purchase in market.
The present invention will be further explained with reference to the following examples:
1) Preparation of sample pad: the glass fiber membrane is prepared by soaking a glass fiber membrane in a buffer solution and then drying the soaked glass fiber membrane, and the preparation method comprises the following steps: soaking the sample pad in the sample pad treating solution for more than 15 minutes, placing the sample pad in a drying oven or drying room at 37 ℃, and drying the sample pad for more than 16 hours. The preparation method of the sample pad treatment solution comprises the following steps: 38.137g of Na were weighed2B4O7·10H2O, 10g PVP, 1g BSA, 10mL Triton X-100, plus 1L H2Dissolving O, and adjusting the pH value to 9.3.
2) Preparing a colloidal gold bonding pad: the glass fiber membrane is prepared by soaking a glass fiber membrane in a buffer solution and then drying the soaked glass fiber membrane, and the preparation method comprises the following steps: sample for useSoaking the pad treatment solution for more than 30 minutes, placing in a drying oven or drying room at 37 ℃, and drying for more than 16 hours. The preparation method of the gold-labeled antibody combined pad treatment solution comprises the following steps: 5g of PVA and 7.1g of Na were weighed2HPO45g BSA, 1mL Triton X-100, plus 1L H2Dissolving O, and adjusting the pH value to 7.4. Preparing colloidal gold particles, marking the antibody combined with the antigen by the colloidal gold particles, determining the spraying amount of the gold-labeled antibody according to repeated debugging, and coating the gold-labeled antibody on the combination pad by a gold spotting machine.
3) Preparation of the cushion: soaking glass fiber membrane in 0.1mol/L Phosphate Buffer Solution (PBS), 1% polyethylene glycol octyl phenyl ether (Triton X-100) and 1% Bovine Serum Albumin (BSA) mixed solution, and air drying.
4) Coating of C line and T line: coating the goat anti-mouse IgG and the specific binding antigen on a nitrocellulose membrane as a C line and a T line by using a film spotting machine according to the working concentration and the spraying amount of the C line goat anti-mouse IgG and the working concentration and the spraying amount of the T line specific binding antigen which are determined by repeated debugging.
5) Coating of amino acid thread: preparing an amino acid solution (0.1% of leucine, 0.1% of phenylalanine, 0.2% of threonine, 0.1% of lysine, 0.1% of aspartic acid and the balance of water), coating the amino acid solution at one end of a reaction membrane close to a colloidal gold bonding pad to obtain an amino acid line, wherein the distance between the amino acid line and the detection line is 4-6 mm;
6) assembling the reagent strip: the sample pad, the colloidal gold combined pad, the buffer pad, the nitrocellulose membrane and the water absorption pad are sequentially adhered to the PVC substrate to form a reagent card, and the reagent card is cut into test paper strips by a cutting machine. The structure is schematically shown in figure 1.
Referring to fig. 1, the colloidal gold test strip of the present invention comprises a bottom plate 10 and a sample pad 1, a colloidal gold combination pad 2, a buffer pad 3, a reaction membrane 4, a water absorption pad 8 and a non-setting adhesive 9 which are sequentially and tightly connected on the bottom plate 10; the colloidal gold bonding pad is coated with a labeled antibody which is combined with the veterinary drug and illegal additives; the buffer pad 3 is coated with buffer solution; an amino acid line 5, a detection line 6 and a quality control line 7 are sequentially arranged on the reaction membrane 4 along the chromatography direction; the amino acid thread 5 is coated with an amino acid solution; the detection line 6 is coated with an artificially synthesized antigen; the quality control line 7 is coated with a goat anti-mouse IgG secondary antibody which can be combined with the labeled antibody.
The utility model discloses the operation flow of colloidal gold reagent board is shown in figure 2, and wherein, S shows the application hole, and 6 show detection lines, 7 show matter accuse line, and 11 show plastic casing, 12 show the observation window.
The interpretation method of the colloidal gold test strip of the utility model is shown in figure 3, wherein T is a detection line 6, and C is a quality control line 7; when the sample does not contain the detected veterinary drug and illegal additives or the contents of the veterinary drug and the illegal additives are lower than the detection limit, the color development of the T line of the test strip is stronger than or equal to that of the C line, and the test strip is negative; the T line is positive without color development or weaker than the C line; the C line did not develop color, and the test strip was ineffective regardless of whether T developed color or not.
Respectively with aflatoxin B1, clenbuterol hydrochloride, gram-chloramphenical, furazolidone as the antigen that awaits measuring, prepare according to embodiment 1's method and obtain the utility model is used for detecting four colloidal gold test paper strips of aflatoxin B1, clenbuterol hydrochloride, chloramphenicol, furazolidone, the structure is shown in figure 1.
Four traditional colloidal gold test strips for detecting aflatoxin B1, clenbuterol hydrochloride, chloramphenicol and furazolidone metabolites are prepared, and the structural schematic diagram is shown in figure 4.
The conventional colloidal gold test strip and the colloidal gold test strips of the embodiments 2 to 5 are used for detecting aflatoxin B1, clenbuterol hydrochloride, chloramphenicol and furazolidone metabolites respectively, and the results are shown in the figures 6 to 9.
Can know by figure 6-9, compare traditional colloidal gold test paper strip, the utility model discloses the colour development is more clear, even after the colloidal gold test paper strip adds the amino acid line, is applicable to aflatoxin B1, clenbuterol hydrochloride, chloramphenicol, the detection of furazolidone metabolite.
Example 7 optimization of amino acid lines coated with amino acid solutions of different concentrations
And (3) optimizing the concentration of the amino acid solution by taking chloramphenicol as an agricultural and veterinary drug to be detected and an illegal additive. The influence of the concentrations of the five amino acid solutions in the amino acid solution on the color development of the chloramphenicol test strip when the concentrations of the five amino acid solutions are all 0.05%, 0.1% and 0.2% is examined. The test strips were prepared as described in example 1. The results are shown in FIG. 5.
Experimental results show that the colloidal gold test strip prepared by coating the amino acid line with 0.1% of amino acid solution has clear and more uniform color development of the T line.
Example 8 precision testing experiment
The aflatoxin B1 was used as the antigen to be detected, the colloidal gold test strip for detecting aflatoxin B1 was prepared according to the method of example 1, aflatoxin B1 solutions with detection concentrations of 0ppb, 0.5ppb, 1ppb, 2ppb, 4pp, and 8ppb, and the detection data was determined with a colloidal gold reader. From the 4 measurements, the Coefficient of Variation (CV) was calculated.
Meanwhile, a sample to be detected is detected by using a traditional colloidal gold test strip (the structure is shown in figure 4), and the result is shown in tables 1-2.
TABLE 1 test results of conventional colloidal gold test strips
TABLE 2 the test result of the colloidal gold test strip
Can know by the testing result of table 1 ~ 2, the utility model discloses the coefficient of variation of colloidal gold test paper strip is between 1.5 ~ 7.2%, obviously is less than the coefficient of variation of traditional colloidal gold test paper strip, shows the utility model discloses the test paper strip has higher accuracy.
Taking a chloramphenicol immune colloidal gold reagent plate as an example, comparing the sensitivity of the traditional reagent plate without using a buffer pad (structure shown in figure 4) and the reagent plate using a buffer pad in the application example 1 (structure shown in figure 1), adding a chloramphenicol standard solution to the negative solution to a final concentration of 0.05, 0.1, 0.2 and 0.4. mu.g/kg, titrating the reagent plate with the buffer pad and the reagent plate without the buffer pad respectively, and measuring the detection data and the detection result by using a colloidal gold reader, wherein the experimental result is shown in table 3.
TABLE 3 sensitivity test results
As can be seen from the experimental data in Table 3, the utility model discloses the detection limit of using the reagent board that has the blotter is obviously less than the detection limit of not using the traditional reagent board of blotter.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. A colloidal gold test strip for detecting veterinary drugs and illegal additives is characterized by comprising a bottom plate and a sample pad, a colloidal gold combination pad, a buffer pad, a reaction membrane, a water absorption pad and a non-setting adhesive which are sequentially and tightly connected on the bottom plate;
the colloidal gold bonding pad is coated with a labeled antibody which is combined with the veterinary drug and illegal additives; the buffer pad is coated with buffer solution; an amino acid line, a detection line and a quality control line are sequentially arranged on the reaction membrane along the chromatography direction; the amino acid coil is coated with an amino acid solution; the detection line is coated with an artificially synthesized antigen; and the quality control line is coated with a goat anti-mouse IgG secondary antibody which can be combined with the labeled antibody.
2. The colloidal gold test strip of claim 1, wherein the labeled antibody is a monoclonal antibody labeled with colloidal gold.
3. The colloidal gold test strip of claim 1, wherein the synthetic antigen is a conjugate of the veterinary drug and illegal additives and a carrier protein.
4. The colloidal gold test strip of claim 3, wherein the carrier protein is one of bovine serum albumin, ovalbumin, and hemocyanin.
5. The colloidal gold test strip of claim 1, wherein the veterinary drug and illegal additive is aflatoxin B1, chloramphenicol, clenbuterol hydrochloride, or furazolidone metabolite.
6. The colloidal gold test strip of claim 1, wherein the distance between the amino acid line and the detection line is 4-6 mm.
7. The colloidal gold test strip of claim 1, wherein the base plate is a PVC sheet.
8. The colloidal gold test strip of claim 1, wherein the sample pad and the colloidal gold conjugate pad are made of glass fiber.
9. The colloidal gold test strip of claim 1, wherein the reaction membrane is an NC membrane.
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| CN201922485723.6U CN211292934U (en) | 2019-12-31 | 2019-12-31 | Colloidal gold test strip for detecting pesticide and veterinary drug and illegal additives |
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