CN211284346U - Be used for influenza virus and symbiotic bacteria concentration collection device in lake water - Google Patents
Be used for influenza virus and symbiotic bacteria concentration collection device in lake water Download PDFInfo
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- CN211284346U CN211284346U CN201921997266.2U CN201921997266U CN211284346U CN 211284346 U CN211284346 U CN 211284346U CN 201921997266 U CN201921997266 U CN 201921997266U CN 211284346 U CN211284346 U CN 211284346U
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Abstract
The utility model relates to a concentrated collecting device for influenza virus and symbiotic bacteria in lake water, which comprises a collector, a water pump and a magnetic adsorption column, wherein the magnetic adsorption column can adsorb or loosen magnetic beads for adsorbing the influenza virus and the symbiotic bacteria; the front end of collector is equipped with the touch-sensitive screen, inside is equipped with the suction pump, its one side is equipped with water inlet and delivery port, the mouth of pipe that absorbs water of suction pump communicates the water inlet, the rear end of collector is equipped with the support, but be equipped with the axis of rotation on the support, rotatable epaxial U type frame that is equipped with, the both ends opening part of U type frame all is equipped with the connecting pipe, the connecting pipe is linked together with magnetism adsorption column joint, the outlet pipe mouth of pipe intercommunication U type frame's of suction pump one end, the other end intercommunication collector's of U type frame delivery port, start-up and the stopping of suction. The utility model discloses the device can effectively catch the virus in the lake water, carries out accurate detection to the target microorganism that the immunomagnetic bead was caught through other detections, and a large amount of lake waters of its effective solution are difficult to the examination pathogen detection and waste time and energy scheduling problem.
Description
Technical Field
The utility model relates to a be used for influenza virus and symbiotic bacteria concentration collection device in lake water belongs to laboratory test equipment technical field.
Background
Influenza viruses cause acute respiratory infectious diseases, are spread rapidly and widely, have three types, namely type A, type B and type C, can infect humans, all human influenza viruses can cause avian influenza, but not all avian influenza viruses can cause human influenza, and the avian influenza viruses which can infect humans are confirmed to be 8 in total, including H5N1, H5N2, H7N2, H7N3, H7N4, H7N7, H9N2, H10N8 and H7N 9. Waterfowls are reported in the literature as "mixers" of influenza viruses, and new influenza viruses infect poultry through the habitat of the poultry through reassortment in the waterfowl, and recently, it has been reported that toxic excretions of waterfowls can be transmitted through polluted river water and lake water. At present, the influenza viruses existing in river water and lake water and other water-borne pathogens have the characteristic of being difficult to detect and capture, and meanwhile, the symbiotic relationship between the influenza viruses and microorganisms in the water environment is beneficial to the survival and the propagation of the influenza viruses, so that the detection of the influenza viruses and the symbiotic microorganisms thereof in the lake water where the poultry live has important practical significance on the storage and the propagation of the influenza viruses, epidemic early warning of epidemic situations and the development and the production of vaccines.
Laboratory diagnostic methods for influenza virus include serological diagnosis, virus cell culture, and antigen detection. The virus can be separated and identified by infecting sensitive tissue cells, which can be used as the most direct and reliable basis for influenza virus infection. In addition, a 4-fold increase in acute phase serum over convalescent phase serum antibodies is also a criterion for influenza infection. However, both of them take a long time, and generally, 3d to 4d are required for cell culture, and half a month is required for serological examination. Lake water belongs to a mobile phase, and virus is not easy to capture in the lake water, so that the detection purpose is realized by adopting the lake water to culture virus cells at present, time and experimental reagents are consumed, and the detection is easy to miss, so that the detection result cannot be accurately obtained. How to realize the effective capture and detection of influenza virus in lake water is urgent.
SUMMERY OF THE UTILITY MODEL
Technical problem to be solved
In order to solve the problems in the prior art, the invention provides a device for collecting and concentrating influenza viruses and symbiotic bacteria in lake water.
(II) technical scheme
In order to achieve the above object, the utility model discloses a main technical scheme include:
a concentrated collection device for influenza viruses and symbiotic bacteria in lake water comprises a collector, a liquid pump and a magnetic adsorption column, wherein the middle of the magnetic adsorption column is hollow, and can adsorb or release immunomagnetic beads for adsorbing the influenza viruses and the symbiotic bacteria; the front end of collector is equipped with the touch-sensitive screen, the inside of collector is equipped with the drawing liquid pump, one side of collector is equipped with water inlet and delivery port, the mouth of pipe intercommunication water inlet that absorbs water of drawing liquid pump, the rear end of collector is equipped with the support, but be equipped with the axis of rotation on the support, rotatable epaxial U type frame that is equipped with, U type frame is the cavity setting, the both ends opening part of U type frame all is equipped with the connecting pipe, the connecting pipe is with the hollow cavity of magnetism adsorption column joint and intercommunication magnetism adsorption column, the outlet pipe mouth of pipe intercommunication U type frame of drawing liquid pump, the other end intercommunication collector's of U type frame delivery port, start-up and stop through the drawing.
In a preferred embodiment, the U-shaped frame is vertically rotatable on a rotatable shaft.
In a preferred embodiment, the U-shaped frame is elastically arranged, and the two open ends of the U-shaped frame are pressed and clamped tightly by the elastic force of inward pressing.
In a preferred embodiment, a hollow cavity of the magnetic adsorption column is internally provided with a prefabricated immunomagnetic bead for absorbing influenza viruses and symbiotic bacteria.
In a preferred embodiment, a detachable annular neodymium iron boron magnet is arranged in the magnetic adsorption column, and the middle of the neodymium iron boron magnet is arranged in a hollow mode.
In a preferred embodiment, an electromagnet is fixedly arranged in the magnetic adsorption column, and a direct current switch and an alternating current switch of the electromagnet are controlled by a touch screen.
In a preferred embodiment, the magnetic adsorption column is provided with a miniature motor and an eccentric wheel, the motor drives the eccentric wheel to rotate at a high speed to enable the magnetic adsorption column to vibrate horizontally, and the motor is controlled by a touch screen to switch.
Wherein, miniature motor and an eccentric wheel can be established in arbitrary position or the U type frame of magnetism adsorption column, and the starter motor will drive magnetism adsorption column and take place the horizontal direction vibration, make the immunomagnetic bead mixing in the hollow cavity of magnetism adsorption column.
In a preferred embodiment, both ends of the magnetic adsorption column are provided with pluggable cards, and the cards seal the hollow cavity of the magnetic adsorption column.
(III) advantageous effects
The invention has the beneficial effects that:
the device of the utility model can be automaticCollecting large volume of lake water, and performing the steps of collecting influenza virus and symbiotic bacteria thereof in the lake water by 108The device is concentrated and collected, the immunomagnetic beads can be eluted and separated from the device after collection, target microorganisms captured by the immunomagnetic beads are accurately detected through other detections, and influenza viruses and symbiotic bacteria thereof in concentrated samples can be cultured and identified. The utility model discloses a large amount of lake water that the device effectively solved is difficult to the examination pathogen and detects and waste time and energy scheduling problem. The utility model discloses the device still can be used to catch required pathogenic bacterium in other waters, only needs to change the pathogen capture thing of immunomagnetic bead peridium.
Drawings
FIG. 1 is a schematic view of the overall apparatus of the present invention;
fig. 2 shows the structure of the magnetic adsorption column.
[ description of reference ]
1: a collector;
2: a magnetic adsorption column;
3: a touch screen;
4: a water inlet;
5: a water outlet;
6: a rotatable shaft;
7: a support;
8: a U-shaped frame.
Detailed Description
For the purpose of better explaining the present invention and to facilitate understanding, the present invention will be described in detail by way of specific embodiments with reference to the accompanying drawings.
Example 1
A device for concentrating and collecting influenza viruses and symbiotic bacteria in lake water comprises a collector 1, a liquid pump and a magnetic adsorption column 2, wherein the magnetic adsorption column 2 can adsorb or release magnetic beads for adsorbing the influenza viruses and the symbiotic bacteria; the middle of the magnetic adsorption column 2 is hollow, the hollow cavity is used for placing immunomagnetic beads for adsorbing influenza viruses and symbiotic bacteria, as shown in fig. 2, the front end of the collector 1 is provided with a touch screen 3, and the touch screen is provided with a liquid pumping key, an enrichment key and an oscillation key; the liquid pumping key is communicated with a liquid pumping pump, the liquid pumping pump starts to work when the liquid pumping key is pressed for the first time, the liquid pumping key is pressed again, the liquid pumping pump stops working, the liquid pumping pump is arranged inside the collector 1, one side of the collector 1 is provided with a water inlet 4 and a water outlet 5, a water suction pipe port of the liquid pumping pump is communicated with the water inlet 4, the rear end of the collector is provided with a support 7, the support is provided with a rotatable shaft 6, a water outlet pipe of the liquid pumping pump is communicated with the rotatable shaft 6, the rotatable shaft 6 is provided with a U-shaped frame 8, and the U-shaped frame 8 can rotate on the rotatable; the U type both ends opening part of U type frame inwards all is equipped with the connecting pipe, and U type frame is the cavity setting, and the intercommunication connecting pipe, connecting pipe and magnetism adsorption column joint communicate, and the outlet pipe mouth of pipe of drawing liquid pump communicates the one end of U type frame through rotatable axle, and U type frame one end communicates the other end that magnetism adsorption column put up to U type, and the other end of U type frame passes through the delivery port of rotatable axle intercommunication collector, through the start-up and the stop of drawing liquid key control drawing liquid pump on the touch-sensitive screen. And the liquid pump is started, so that lake water or water can enter from the water inlet and flow through the liquid pump, one end of the U-shaped frame, the connecting pipe at one end, the hollow cavity of the magnetic adsorption column, the connecting pipe at the other end, the other end of the U-shaped frame and finally flow out from the water outlet. The middle of the magnetic adsorption column 2 is arranged in a hollow way, and the magnetic adsorption column 2 can adsorb or loosen immunomagnetic beads for adsorbing influenza viruses and symbiotic bacteria. The immunomagnetic beads are adsorbed on the inner wall of the middle of the magnetic adsorption column 2 through magnetic force; when there is no magnetic force, the immunomagnetic beads will fall freely in the hollow space in the middle of the magnetic adsorption column 2. Specifically, be equipped with detachable annular neodymium iron boron magnet in the magnetism adsorbs post 2, be the cavity setting in the middle of the neodymium iron boron magnet, the centre of magnetism adsorbs post 2 also is the cavity setting, puts into magnetism adsorbs post 2 with annular neodymium iron boron magnet in, can adsorb the immunomagnetic bead on the inner wall of the centre of magnetism adsorbs post 2, when taking out annular neodymium iron boron magnet, the magnetic field in the magnetism adsorbs post 2 disappears, and the immunomagnetic bead then can freely drop.
The during operation, place neodymium iron boron magnet in the magnetic adsorption post, place the prefabricated immunomagnetic bead that is used for absorbing influenza virus and symbiotic bacteria in the hollow cavity of magnetic adsorption post, immunomagnetic bead adsorbs in hollow cavity this moment, when the drawing liquid pump is opened, can enter into the magnetic adsorption post with water or lake water through 4 pump inlets to the one end of U type frame, adsorb influenza virus and other symbiotic bacteria in the lake water on the immunomagnetic bead through immunomagnetic bead's adsorption to reach the effect of adsorbing enrichment lake aquatic virus.
The lake water passing through the magnetic adsorption column flows out from the other end, enters the other end of the U-shaped frame and is reserved from the water outlet. During the detection, close the drawing liquid pump, drawing liquid pump stop work takes off the magnetism adsorption column, and the washing of usable sterile water takes out neodymium iron boron magnetism iron boron, and the immunomagnetic bead can be taken out from the magnetism adsorption column along with sterile water together. Then the immunomagnetic beads are used for ELISA detection, namely the situation of carrying viruses in lake water can be detected, and the 10 th detection of influenza viruses and symbiotic bacteria thereof in the lake water is realized8Concentrating and collecting.
Further, in order to make the testing result more accurate, can make U type frame rotate in the vertical direction through manual rotation with U type frame on the rotatable shaft for lake water is more abundant with the immunomagnetic bead contact, and the virus is changeed and is fully combined with the immunomagnetic bead, and the enrichment is more effective, makes the detection more accurate.
In order to enable the magnetic adsorption column to be more stable and not easy to fall off, the U-shaped frame is arranged to be elastic, and the two open ends of the U-shaped frame are clamped and connected with the magnetic adsorption column through inward-pressing elastic force.
Be equipped with miniature motor (motor) and an eccentric wheel on magnetism adsorbs the post, motor drive eccentric wheel rotates at a high speed, this in-process eccentric wheel can be circular motion and produce centrifugal force, and the direction of centrifugal force can be along with the rotation of eccentric wheel and constantly change, the quick change of centrifugal force also leads to whole motor all at quick shake simultaneously, finally drive magnetism and adsorb the post and take place horizontal direction's vibration about, be equipped with the vibration key on the touch-sensitive screen, the vibration key communicates miniature motor, the start-up and the closing of control motor, thereby the horizontal vibration of control magnetism adsorbs the post.
After the magnetic bead enrichment is finished, the hollow portion of magnetic adsorption post does not have lake water this moment, upper and lower both ends at magnetic adsorption post are equipped with pluggable card, insert the card, two cards are sealed hollow portion, can take off magnetic adsorption post, add sterile water or lotion at hollow portion, take out neodymium iron boron magnet, install magnetic adsorption post on U type frame again, press the vibration key, magnetic adsorption post can horizontal vibration, play the purpose of wasing the immunomagnetic bead, take off magnetic adsorption post again, install neodymium iron boron magnet, can pour sterile water or lotion this moment, play the purpose of wasing the immunomagnetic bead, add sterile water again, take off neodymium iron boron magnet, pour the sterile water that contains the immunomagnetic bead, be used for further detection.
Example 2
This embodiment is on embodiment 1's basis, in order to make the effect of enrichment better, can the automation mechanized operation, can replace the electro-magnet with detachable neodymium iron boron magnet. The touch screen is also provided with a direct current switch, namely an enrichment key, for controlling the electromagnet, the electromagnet is matched with the magnetic adsorption column in size and shape, the middle part of the electromagnet is arranged in a hollow way, and the electromagnet is embedded in the magnetic adsorption column. The connecting direct current forms the effect of super-paramagnetic field through the electro-magnet, can make the immunomagnetic bead adsorb on the hollow inner wall of magnetism adsorption column fast, effectively accomplishes the enrichment at a high speed. When the direct current switch is communicated, the enrichment key is pressed, the electromagnet plays a magnet role and is used for adsorbing the immunomagnetic beads, the direct current switch is closed by pressing the enrichment key again, and the immunomagnetic beads are in a free state. An alternating current switch, namely a mixing key, for controlling the electromagnet is also arranged on the touch screen, and the rotating direction of the magnetic beads in the liquid in the collecting pipe can be changed by changing the direction of the electrode through alternating current, so that pathogens are more fully enriched. The electromagnet can change the rotation direction of the immunomagnetic beads in the liquid of the collecting pipe and is controlled by the alternating current switch, so that the immunomagnetic beads can be uniformly mixed in the solution.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in other forms, and any person skilled in the art can change or modify the technical content disclosed above into an equivalent embodiment with equivalent changes. However, any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the protection scope of the technical solution of the present invention.
Claims (8)
1. A device for concentrating and collecting influenza viruses and symbiotic bacteria in lake water is characterized by comprising a collector, a liquid pump and a magnetic adsorption column, wherein the middle of the magnetic adsorption column is hollow and can adsorb or loosen magnetic beads for adsorbing the influenza viruses and the symbiotic bacteria; the front end of collector is equipped with the touch-sensitive screen, one side that the inside of collector was equipped with the drawing liquid pump collector is equipped with water inlet and delivery port, the suction pipe mouth intercommunication water inlet of drawing liquid pump, the rear end of collector is equipped with the support, but be equipped with the axis of rotation on the support, rotatable epaxial U type frame that is equipped with, U type frame is the cavity setting, the both ends opening part of U type frame all is equipped with the connecting pipe, the connecting pipe is with the hollow cavity of magnetism adsorption column joint and intercommunication magnetism adsorption column, the outlet pipe mouth of drawing liquid pump communicates the one end of U type frame, the other end intercommunication collector's of U type frame delivery port, start-up and stop through touch-sensitive.
2. The apparatus for collecting concentrated influenza virus and symbiotic bacteria in lake water according to claim 1, wherein the U-shaped frame is vertically rotatable on a rotatable shaft.
3. The device for collecting influenza virus and symbiotic bacteria in lake water according to claim 1, wherein the U-shaped frame is elastically arranged, and the magnetic adsorption column is tightly clamped by the elastic force of inward pressing at the two open ends of the U-shaped frame.
4. The device for collecting the concentrated influenza virus and the symbiotic bacteria in the lake water according to claim 1, wherein a pre-prepared immunomagnetic bead for absorbing the influenza virus and the symbiotic bacteria is arranged in the hollow cavity of the magnetic adsorption column.
5. The device for collecting and concentrating influenza viruses and symbiotic bacteria in lake water according to claim 1, wherein a detachable annular neodymium-iron-boron magnet is arranged in the magnetic adsorption column, and the middle of the neodymium-iron-boron magnet is hollow.
6. The device for collecting influenza viruses and symbiotic bacteria in lake water according to claim 1, wherein an electromagnet is fixedly arranged in the magnetic adsorption column, and a direct current switch and an alternating current switch of the electromagnet are controlled by a touch screen.
7. The device for collecting influenza virus and symbiotic bacteria in lake water according to claim 1, wherein the magnetic adsorption column is provided with a micro motor and an eccentric wheel, the motor drives the eccentric wheel to rotate at high speed to enable the magnetic adsorption column to vibrate horizontally, and the motor is controlled by a touch screen to be switched on and off.
8. The device for collecting the concentrated influenza virus and the symbiotic bacteria in the lake water according to claim 1, wherein the two ends of the magnetic adsorption column are provided with pluggable cards, and the hollow cavity of the magnetic adsorption column is sealed by the pluggable cards.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921997266.2U CN211284346U (en) | 2019-11-19 | 2019-11-19 | Be used for influenza virus and symbiotic bacteria concentration collection device in lake water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921997266.2U CN211284346U (en) | 2019-11-19 | 2019-11-19 | Be used for influenza virus and symbiotic bacteria concentration collection device in lake water |
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| Publication Number | Publication Date |
|---|---|
| CN211284346U true CN211284346U (en) | 2020-08-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921997266.2U Active CN211284346U (en) | 2019-11-19 | 2019-11-19 | Be used for influenza virus and symbiotic bacteria concentration collection device in lake water |
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| Country | Link |
|---|---|
| CN (1) | CN211284346U (en) |
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2019
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