CN211284338U - PCR sealing machine - Google Patents
PCR sealing machine Download PDFInfo
- Publication number
- CN211284338U CN211284338U CN201921846658.9U CN201921846658U CN211284338U CN 211284338 U CN211284338 U CN 211284338U CN 201921846658 U CN201921846658 U CN 201921846658U CN 211284338 U CN211284338 U CN 211284338U
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- Prior art keywords
- fixedly connected
- wall
- movable
- frame
- pcr
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- 238000007789 sealing Methods 0.000 title claims abstract description 19
- 238000010438 heat treatment Methods 0.000 claims abstract description 29
- 238000003825 pressing Methods 0.000 claims abstract description 24
- 238000005485 electric heating Methods 0.000 claims description 5
- 239000003381 stabilizer Substances 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 11
- 239000011148 porous material Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 5
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000010030 laminating Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007731 hot pressing Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
Images
Abstract
The utility model discloses a PCR sealing machine, including frame, rack and mount, the top of frame and the bottom fixed connection of mount, the rack is located the mount under to the bottom of rack and the top fixed connection of frame, the utility model relates to a sealing machine technical field. This PCR sealing machine, sliding connection has the heating platen between the both sides through the mount inner wall, the mounting groove has been seted up to the inside of mount, and the servo electric jar of bottom fixedly connected with of mounting groove inner wall, the equal fixedly connected with slider in heating platen's both sides, and the front of slider and the inner wall sliding connection of the back and mounting groove, utilize servo electric jar output shaft to drive the heating platen downstream, replace the artifical mode of pressing down the heating platen, staff's intensity of labour has not only been reduced, the convenient pressing force degree to the heating platen simultaneously controls the regulation, it is better to make the sealed effect of heating platen to 96 orifice plates, excellent in practical effect.
Description
Technical Field
The utility model relates to a sealing machine technical field specifically is a PCR sealing machine.
Background
The PCR is carried out by changing the denaturation of DNA into single strand at 95 deg.C in vitro, combining primer and single strand at low temperature according to the base complementary pairing principle, regulating temperature to the optimum reaction temperature of DNA polymerase, synthesizing complementary strand by DNA polymerase along the direction from phosphoric acid to pentose, and controlling the denaturation, renaturation and extension temperature.
The name that current open patent number is CN204569896U is "a PCR sealing machine", including base and support frame, the front installation of base has time to set up device and temperature setting device, and the display screen is installed to the top of time setting device and temperature setting device, be provided with 96 pore grillages directly over the base, on 96 pore grillages were arranged in to the 96 pore plate that will paste the membrane in this scheme, manual pressing heat pressure handle to the 96 pore grillages of placing 96 pore plate on, utilize manual mode to carry out pressing of hot pressure handle, when increase staff's intensity of labour, still can lead to the laminating effect unsatisfactory because the pressing force degree is not enough easily, relatively poor to the sealed effect of 96 pore plate, the practical function is relatively poor.
SUMMERY OF THE UTILITY MODEL
The not enough to prior art, the utility model provides a PCR sealing machine has solved and has utilized manual mode to carry out pressing of hot pressing handle among patent number CN204569896U, when increase staff's intensity of labour, still can lead to the laminating effect unsatisfactory because the pressing force degree is not enough easily, and is relatively poor to the sealed effect of 96 orifice plates, the relatively poor problem of practical effect.
In order to achieve the above purpose, the utility model discloses a following technical scheme realizes: a PCR sealing machine comprises a machine base, a placing rack and a fixing frame, wherein the top of the machine base is fixedly connected with the bottom of the fixing frame, the placing rack is positioned under the fixing frame, the bottom of the placing rack is fixedly connected with the top of the machine base, a heating pressing plate is slidably connected between the two sides of the inner wall of the fixing frame, a mounting groove is formed in the fixing frame, a servo electric cylinder is fixedly connected with the bottom of the inner wall of the mounting groove, sliding blocks are fixedly connected with the two sides of the heating pressing plate, the front surface and the back surface of each sliding block are slidably connected with the inner wall of the mounting groove, the top end of an output shaft of the servo electric cylinder is fixedly connected with the bottom of each sliding block, a placing groove is formed in the top of the placing rack, limiting plates are slidably connected with the front surface and the two sides of the, the movable groove is formed in the placing rack, the movable rod is fixedly connected with a movable plate on the surface inside the movable groove, one side of the movable plate is fixedly connected with a spring, one end of the spring is fixedly connected with the back of the inner wall of the movable groove, and one end of the movable rod is fixedly connected with a pull rod.
Preferably, a movable sliding groove matched with the sliding block is formed in one side of the fixing frame, and the front face and the back face of the sliding block are connected with the inner wall of the movable sliding groove in a sliding mode.
Preferably, the front side and the back side of the inner wall of the placing groove are both provided with limiting chutes matched with the limiting plates, and the two sides of each limiting plate are slidably connected with the two sides of the inner wall of each limiting chute.
Preferably, the front of the top of the base is respectively provided with a temperature controller, a display screen and a control key.
Preferably, an electric heating wire is arranged inside the heating pressure plate, and the temperature controller is electrically connected with the electric heating wire.
Preferably, the periphery of the bottom of the machine base is fixedly connected with support legs, and the bottoms of the support legs are provided with anti-slip pads.
Advantageous effects
The utility model provides a PCR sealing machine. Compared with the prior art, the method has the following beneficial effects:
(1) this PCR sealing machine, sliding connection has the heating platen between the both sides through the mount inner wall, the mounting groove has been seted up to the inside of mount, and the servo electric jar of bottom fixedly connected with of mounting groove inner wall, the equal fixedly connected with slider in heating platen's both sides, and the front of slider and the inner wall sliding connection of the back and mounting groove, the top of servo electric jar output shaft and the bottom fixed connection of slider, utilize servo electric jar output shaft to drive the heating platen downstream, replace the artifical mode of pressing the heating platen, staff's intensity of labour has not only been reduced, simultaneously can also be convenient control the regulation to the pressing force degree of heating platen, make the sealed effect of heating platen to 96 orifice plates better, the practical function is better.
(2) This PCR sealing machine, the standing groove has been seted up through the top of rack, the standing groove inner wall openly has the limiting plate with the equal sliding connection in both sides at the back, the positive fixedly connected with movable rod of limiting plate, and the one end of movable rod runs through the rack, the movable groove has been seted up to the inside of rack, the movable rod is located the fixed surface of movable inslot portion and is connected with the fly leaf, one side fixedly connected with spring of fly leaf, and the one end of spring and the back fixed connection of movable inslot inner wall, the one end fixedly connected with pull rod of movable rod, such device structural design lets 96 orifice plate when the rack is inside to be placed, utilize the limiting plate to carry on spacingly all around at 96 orifice plate top, make placing of 96 orifice plate more stable, thereby.
Drawings
FIG. 1 is a front view of the structure of the PCR sealing machine of the present invention;
FIG. 2 is a cross-sectional view of the fixing frame structure of the present invention;
fig. 3 is a top view of the internal structure of the rack of the present invention;
fig. 4 is a partially enlarged view of a portion a in fig. 3 according to the present invention.
In the figure: the device comprises a machine base 1, a placing frame 2, a fixing frame 3, a heating pressing plate 4, a mounting groove 5, a servo electric cylinder 6, a sliding block 7, a placing groove 8, a limiting plate 9, a movable rod 10, a movable groove 11, a movable plate 12, a spring 13, a pull rod 14, a movable sliding groove 15, a limiting sliding groove 16, a temperature controller 17, a display screen 18, a control button 19 and a support leg 20.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution: a PCR sealing machine comprises a machine base 1, a placing frame 2 and a fixing frame 3, wherein support legs 20 are fixedly connected to the periphery of the bottom of the machine base 1, anti-slip pads are arranged at the bottoms of the support legs 20, a temperature controller 17, a display screen 18 and control keys 19 are respectively arranged on the front side of the top of the machine base 1, a movable sliding chute 15 matched with a sliding block 7 is formed in one side of the fixing frame 3, the front side and the back side of the sliding block 7 are in sliding connection with the inner wall of the movable sliding chute 15, the top of the machine base 1 is fixedly connected with the bottom of the fixing frame 3, the placing frame 2 is positioned under the fixing frame 3, the bottom of the placing frame 2 is fixedly connected with the top of the machine base 1, a heating pressing plate 4 is connected between two sides of the inner wall of the fixing frame 3 in a sliding manner, an electric heating wire is arranged inside the, and the bottom of the inner wall of the mounting groove 5 is fixedly connected with a servo electric cylinder 6, the servo electric cylinder 6 is a modularized product which integrates a servo motor and a lead screw, the speed is 0.1-2 m/s, the control precision is higher because of closed-loop servo control, the cost is low, the configuration is flexible, and the like, both sides of the heating pressing plate 4 are fixedly connected with sliding blocks 7, the front and back surfaces of the sliding blocks 7 are in sliding connection with the inner wall of the mounting groove 5, the top end of an output shaft of the servo electric cylinder 6 is fixedly connected with the bottom of the sliding blocks 7, the heating pressing plate is driven to move downwards by the output shaft of the servo electric cylinder, the mode of manually pressing the heating pressing plate is replaced, the labor intensity of workers is reduced, meanwhile, the pressing force of the heating pressing plate can be conveniently controlled and adjusted, the sealing effect of the heating pressing plate on a 96 pore plate is better, the practical, limiting chutes 16 matched with the limiting plates 9 are formed in the front side and the back side of the inner wall of the placing groove 8, the two sides of each limiting plate 9 are connected with the two sides of the inner wall of the limiting chutes 16 in a sliding manner, the limiting plates 9 are connected with the front side and the back side of the inner wall of the placing groove 8 in a sliding manner, the limiting plates are used for limiting the periphery of the top of the 96-pore plate, the 96-pore plate is placed more stably, the sealing effect of the 96-pore plate is improved, the front side of each limiting plate 9 is fixedly connected with a movable rod 10, one end of each movable rod 10 penetrates through the placing frame 2, a movable groove 11 is formed in the placing frame 2, a movable plate 12 is fixedly connected to the surface of the movable rod 10 inside the movable groove 11, a spring 13 is fixedly connected to one side of the movable plate 12, one end of the spring 13 is fixedly connected with the back side of the inner wall of the movable groove 11 .
When the device is used, the two pull rods 14 are respectively pulled to the front side and the back side, the pull rods 14 drive the movable rod 10 to move in the movable groove 11, one end of the movable rod 10 pulls the limit plate 9 to slide into the limit sliding groove 16, at the moment, the 96-hole plate is placed in the placing groove 8 at the top of the placing frame 2, the two pull rods 14 are loosened, the position of the movable rod 10 is reset under the matching action of the spring 13 and the movable plate 12, the limit plate 9 slides out of the limit sliding groove 16, the bottom surface of the limit plate 9 limits the 96-hole plate, the control button 19 is operated to input the pressure which needs to be applied to the 96-hole plate by the heating press plate 4, the control switch of the servo electric cylinder 6 is opened, the output shaft of the servo electric cylinder 6 drives the sliding blocks 7 at the two sides of the heating press plate 4 to slide downwards in the mounting groove 5, the heating press plate 4 seals the top, in addition, the pressing value and the temperature value of the heating platen 4 are displayed on the display screen 18.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a PCR sealing machine, includes frame (1), rack (2) and mount (3), the top of frame (1) and the bottom fixed connection of mount (3), rack (2) are located mount (3) under to the bottom of rack (2) and the top fixed connection of frame (1), its characterized in that: a heating pressing plate (4) is connected between two sides of the inner wall of the fixing frame (3) in a sliding manner, a mounting groove (5) is formed in the fixing frame (3), a servo electric cylinder (6) is fixedly connected to the bottom of the inner wall of the mounting groove (5), sliders (7) are fixedly connected to two sides of the heating pressing plate (4), the front face and the back face of each slider (7) are connected with the inner wall of the mounting groove (5) in a sliding manner, the top end of an output shaft of the servo electric cylinder (6) is fixedly connected with the bottom of each slider (7), a placing groove (8) is formed in the top of the placing frame (2), limiting plates (9) are connected to two sides of the front face and the back face of the inner wall of each placing groove (8) in a sliding manner, a movable rod (10) is fixedly connected to the front face of each limiting plate (9), one end of each movable rod, the surface of the movable rod (10) located inside the movable groove (11) is fixedly connected with a movable plate (12), one side of the movable plate (12) is fixedly connected with a spring (13), one end of the spring (13) is fixedly connected with the back of the inner wall of the movable groove (11), and one end of the movable rod (10) is fixedly connected with a pull rod (14).
2. A PCR sealer as claimed in claim 1, wherein: one side of the fixed frame (3) is provided with a movable sliding chute (15) matched with the sliding block (7), and the front and back of the sliding block (7) are in sliding connection with the inner wall of the movable sliding chute (15).
3. A PCR sealer as claimed in claim 1, wherein: limiting sliding grooves (16) matched with the limiting plates (9) are formed in the front face and the back face of the inner wall of the placing groove (8), and the two sides of the limiting plates (9) are connected with the two sides of the inner wall of the limiting sliding grooves (16) in a sliding mode.
4. A PCR sealer as claimed in claim 1, wherein: the front side of the top of the machine base (1) is respectively provided with a temperature controller (17), a display screen (18) and a control key (19).
5. The PCR sealing machine of claim 4, wherein: an electric heating wire is arranged inside the heating pressing plate (4), and the temperature controller (17) is electrically connected with the electric heating wire.
6. A PCR sealer as claimed in claim 1, wherein: all fixedly connected with stabilizer blade (20) around frame (1) bottom to the bottom of stabilizer blade (20) is provided with the slipmat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921846658.9U CN211284338U (en) | 2019-10-30 | 2019-10-30 | PCR sealing machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921846658.9U CN211284338U (en) | 2019-10-30 | 2019-10-30 | PCR sealing machine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211284338U true CN211284338U (en) | 2020-08-18 |
Family
ID=72037432
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921846658.9U Active CN211284338U (en) | 2019-10-30 | 2019-10-30 | PCR sealing machine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211284338U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113999752A (en) * | 2021-10-20 | 2022-02-01 | 美东汇成生命科技(昆山)有限公司 | Sealed effectual PCR shrouding membrane |
-
2019
- 2019-10-30 CN CN201921846658.9U patent/CN211284338U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113999752A (en) * | 2021-10-20 | 2022-02-01 | 美东汇成生命科技(昆山)有限公司 | Sealed effectual PCR shrouding membrane |
| CN113999752B (en) * | 2021-10-20 | 2023-05-09 | 美东汇成生命科技(昆山)有限公司 | Sealing effect's PCR shrouding membrane |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A PCR sealing machine Granted publication date: 20200818 Pledgee: Guanggu Branch of Wuhan Rural Commercial Bank Co.,Ltd. Pledgor: Aikangjian (Wuhan) Gene Technology Co.,Ltd. Registration number: Y2024980008102 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |