CN211205962U

CN211205962U - Immunohistochemical antibody smearing device

Info

Publication number: CN211205962U
Application number: CN201922171141.0U
Authority: CN
Inventors: 张秋菊; 王奎淞; 赵鲲鹏; 刘靓; 刘静
Original assignee: Gansu University of Chinese Medicine
Current assignee: Gansu University of Chinese Medicine
Priority date: 2019-12-06
Filing date: 2019-12-06
Publication date: 2020-08-07
Anticipated expiration: 2029-12-06

Abstract

The utility model relates to an immunohistochemical antibody experiment, concretely relates to immunohistochemical antibody uniform smearing device, including pole, dish, silica gel brush hair, immunohistochemical thing and slide glass, the dish level set up, the vertical connection of pole is in the dish upper end, the silica gel brush hair sets up in the below of dish, the silica gel brush hair is provided with a plurality ofly, immunohistochemical thing sets up in the slide glass top, immunohistochemical thing passes through the silica gel brush hair and rotates the stirring evenly, still including supporting limiting device, supporting limiting device includes the sleeve, the sleeve top is equipped with the extension board, sleeve lower surface and broken piece contact, immunohistochemical thing sets up in the sleeve, the pole runs through the extension board, pole and extension board swing joint, the dish sets up in the sleeve, can guarantee under the prerequisite of not destroying the tissue that tissue can guarantee that tissue and antibody can paint evenly completely to can guarantee that tissue and antibody do not run off.

Description

Immunohistochemical antibody smearing device

Technical Field

The utility model relates to an immunohistochemical antibody experiment, concretely relates to immunohistochemical antibody uniform smearing device.

Background

In the laboratory carrying out immunohistochemical experimentation, need place the tissue on the slide usually, then drop wise antibody on the tissue, the antibody of dropwise add is because the effect of water molecule tension, and the antibody can not even distribution on the tissue, usually for solving this problem, the operator can use the rifle head of dropwise add the antibody to paint antibody and tissue evenly, but this process has following problem:

firstly, the gun head is made of hard material, so that the tissue is easily damaged in the smearing process, the tissue is scratched, and the experimental result is influenced;

secondly, in the smearing process, because the size of the slide fragment is small, the operation precision cannot be controlled, the gun head is easy to scratch out of the slide glass during operation, and under the tension of antibody water molecules, the antibody brings the tissue out of the slide glass along with the gun head, so that the tissue and the antibody flow outwards, the experiment cannot be carried out, and a new immune composition substance needs to be manufactured again.

SUMMERY OF THE UTILITY MODEL

For overcoming the problem that exists among the above-mentioned prior art, the utility model aims at providing an even ware is smeared to immunohistochemical antibody can guarantee under the prerequisite of not destroying the tissue that tissue and antibody can paint evenly completely to can guarantee that tissue and antibody do not run off, solve the problem that exists among the prior art.

The utility model adopts the technical proposal that: the utility model provides an even ware is smeared to immunohistochemical antibody, includes pole, dish, silica gel brush hair, immunization group thing and slide glass, the setting of dish level, the vertical connection of pole is in the dish upper end, the silica gel brush hair setting is in the below of dish, the silica gel brush hair is provided with a plurality ofly, immunization group thing sets up in the slide glass top, immunization group thing passes through the silica gel brush hair and rotates the stirring.

The device further comprises a supporting and limiting device, the supporting and limiting device comprises a sleeve, a supporting plate is arranged at the top of the sleeve, the lower surface of the sleeve is in contact with the carrying fragment, the immune composition is arranged in the sleeve, the rod penetrates through the supporting plate, the rod is movably connected with the supporting plate, and the disc is arranged in the sleeve.

Further the pole on be equipped with the boss, the boss setting is in the sleeve, boss and extension board lower surface laminating, the pole upper end is connected with presses the cap, presses cap and extension board directly to be equipped with the spring, the pole setting is in the spring, the spring setting is in the extension board top.

Further, the rod and the support plate are connected through threads.

Further the pole on be equipped with spiral groove I, the extension board is equipped with spiral groove II with the pole junction, the pole passes through the ball and is connected with the extension board, the ball setting is between spiral groove I and spiral groove II.

And the support plate is provided with a mounting hole, the mounting hole is communicated with the top of the spiral groove II and the upper part of the support plate, and an opening at the upper end of the mounting hole is connected with a jackscrew.

Furthermore, the support plate is provided with a hole which is communicated with the interior of the sleeve and the upper part of the support plate.

Further a rubber gasket is arranged between the sleeve and the glass slide, the rubber gasket is connected with the lower end of the sleeve, and the immune composition is arranged in the rubber gasket.

The side part of the support plate is provided with two finger handrails, and the finger handrails extend to the outer side of the sleeve.

The utility model has the advantages that:

1. the tissue and the antibody are evenly smeared through the silica gel brush hair, so that the tissue is prevented from being damaged;

2. the sleeve limits the moving range of the silica gel brush hair, and blocks tissues and antibodies to prevent the tissues and the antibodies from flowing outwards;

3. through the connection structure of spring and pole and extension board, realize the silica gel brush hair and can realize synchronous revolution's function at the in-process that descends to rise, help is paintd more evenly fast, realizes self return's function simultaneously, convenient operation, high efficiency is swift.

Drawings

Fig. 1 is a schematic view of an external structure according to an embodiment of the present invention;

FIG. 2 is a schematic view of a second half-section structure of the embodiment of the present invention;

FIG. 3 is a schematic view of a three-and-a-half section structure of an embodiment of the present invention;

fig. 4 is a schematic view of the enlarged structure of a part a of the present invention shown in fig. 3;

FIG. 5 is a schematic view of the cross-sectional structure A-A of the present invention shown in FIG. 3;

FIG. 6 is a schematic top view of the support limiting device of the present invention;

FIG. 7 is a diagram illustrating the effect of mixing a conventional tissue and an antibody.

In the figure: 1. the glass slide bearing comprises a rod, 101, a boss, 102, a pressing cap, 103, a spring, 104, a spiral groove I, 2, a disc, 3, silica gel bristles, 4, an immunohistochemistry substance, 5, a glass slide, 6, a supporting and limiting device, 601, a sleeve, 602, a support plate, 603, a spiral groove II, 604, a ball, 605, a mounting hole, 606, a jackscrew, 607, a hole, 608, a finger handrail and 7 a rubber gasket.

Detailed Description

The present invention will be described in detail with reference to the accompanying drawings and specific embodiments:

in the first embodiment shown in fig. 1: the utility model provides an even ware is smeared to immunohistochemical antibody, includes pole 1, dish 2, silica gel brush hair 3, immunohistochemical thing 4 and

slide glass

5, 2 level settings of dish, 1 vertical connection of pole is in 2 upper ends of dish, silica gel brush hair 3 sets up in the below of dish 2, silica gel brush hair 3 is provided with a plurality ofly, immunohistochemical thing 4 sets up in slide glass 5 top, immunohistochemical thing 4 passes through silica gel brush hair 3 and rotates the stirring, replaces the traditional rifle head that uses the dropwise add antibody through specialized tool, paints antibody and tissue evenly.

When the device is used, an operator holds the rod 1 with two fingers, the silica gel bristles 3 are contacted with the immune composition 4, and then the two fingers twist the rod 1 relatively to rotate, so that the silica gel bristles 3 are driven to rotate along with the rotation, and the effects of stirring and uniformly coating are achieved.

Example two as shown in fig. 2: still include supporting and limiting device 6, supporting and limiting device 6 includes sleeve 601, sleeve 601 top is equipped with extension board 602, sleeve 601 lower surface and broken glass 5 contact, immunohistochemistry thing 4 sets up in sleeve 601, pole 1 runs through extension board 602, pole 1 and extension board 602 swing joint, dish 2 sets up in sleeve 601, carry out the injecing of position through sleeve 601 to pole 1, prevent that it from breaking away from original position at stirring, the even in-process of paining, can restrict immunohistochemistry thing 4 in sleeve 601 simultaneously, and then guaranteed that immunohistochemistry thing 4 can not take place the phenomenon of flowing out from slide 5, pole 1 and extension board 602 swing joint, guaranteed that pole 1 can move up and down relative to extension board 602, and can rotate, do not influence normal stirring, scribble even work.

Further, the rod 1 is provided with the boss 101, the boss 101 is arranged in the sleeve 601, the boss 101 is attached to the lower surface of the support plate 602, the upper end of the rod 1 is connected with the pressing cap 102, the pressing cap 102 and the support plate 602 are directly provided with the spring 103, the rod 1 is arranged in the spring 103, the spring 103 is arranged above the support plate 602, the spring 103 is added to play a role in assisting in resetting, meanwhile, the resistance of the rod 1 in downward movement is increased, the descending distance of the rod 1 can also be limited, the excessive downward movement of the rod 1 is prevented from causing excessive contact between the silica gel bristles 3 and the immune organized object 4, and the compression on the immune organized object 4 is caused, so that the damage to the immune organized object 4 is avoided, the boss 101 can prevent the rod 1 from being separated from the support plate 602, and the limiting and.

The rod 1 and the support plate 602 are connected through threads, the rod 1 can move up and down relative to the support plate 602 and can rotate through the threaded connection, and meanwhile, the position of the rod 1 is further limited, so that the rod 1 is prevented from being inclined to cause uneven stirring.

When the device is used, the sleeve 601 is covered on the immune-histocompound substance 4, the immune-histocompound substance 4 is completely positioned in the sleeve 601, then the rod 1 is screwed to rotate while moving downwards, so that the uniform stirring and smearing work is completed, then the rod 1 is rotated reversely to reset, and the uniform stirring and smearing work is completed again.

Example three as shown in fig. 3-5: pole 1 on be equipped with spiral groove I104, extension board 602 is equipped with spiral groove II 603 with the 1 junction of pole, pole 1 passes through ball 604 and is connected with extension board 602, ball 604 sets up between I104 of spiral groove and II 603 of spiral groove, the connected mode that utilizes ball 604 replaces threaded connection's mode, the motion mode of pole 1 has further been optimized, utilize ball 604 to reduce relative frictional force, carry out the pivoted action when utilizing to press and can easily realize the downward motion of pole 1, utilize spring 103 also to realize quick return function simultaneously, it is more convenient to use.

Further, the support plate 602 is provided with a mounting hole 605, the mounting hole 605 is communicated with the top of the spiral groove II 603 and the upper side of the support plate 602, an opening at the upper end of the mounting hole 605 is connected with a jackscrew 606, and the mounting work of the ball 604 is carried out by utilizing the mounting hole 605, so that the connection and the disassembly of the rod 1 and the support plate 602 are facilitated.

When the device is used, the sleeve 601 is covered on the immunohistochemical substance 4, the immunohistochemical substance 4 is completely positioned in the sleeve 601, the pressing cap 102 is pressed to enable the rod 1 to move downwards and simultaneously rotate, the stirring and smearing uniform work is completed for one time, then the pressing cap 102 is loosened, the rod 1 automatically resets, and the stirring and smearing uniform work is completed for one time.

Further extension board 602 on set up porose 607, inside and extension board 602 top of hole 607 intercommunication sleeve 601, the purpose that increases hole 607 is for the convenience use the utility model discloses a cleaning work prevents to clean not in place, leads to inside to remain the immune component 4 of adhesion when using to pollute new immune component when causing reuse, can stretch out sleeve 601 with silica gel brush hair 3 and wash, disinfect outward during the cleanness, also can wash, disinfect inside sleeve 601 under the condition that needs.

Further be equipped with rubber packing ring 7 between sleeve 601 and slide glass 5, rubber packing ring 7 is connected with sleeve 601 lower extreme, immune composition thing 4 sets up in rubber packing ring 7, increases the frictional force between rubber packing ring 7 multiplicable sleeve 601 and the slide glass 5, the service function of assurance sleeve 601 that can be better, forms cushioning effect simultaneously, prevents to crush slide glass 5.

Further the extension board 602 lateral part be equipped with finger handrail 608 two, finger handrail 608 extends to the sleeve 601 outside, increases finger handrail 608, and the convenience is in the utility model discloses single hand is operated during the use, only needs two fingers of one hand to press or catch on finger handrail 608, can utilize the other finger of this hand to operate pole 1.

Claims

1. An immunohistochemical antibody smearing device is characterized in that: including pole (1), dish (2), silica gel brush hair (3), immunity group thing (4) and slide glass (5), dish (2) level set up, pole (1) vertical connection is in dish (2) upper end, silica gel brush hair (3) set up in the below of dish (2), silica gel brush hair (3) are provided with a plurality ofly, immunity group thing (4) set up in slide glass (5) top, immunity group thing (4) rotate the stirring through silica gel brush hair (3).

2. The immunohistochemical antibody homogenizer according to claim 1, wherein: the device is characterized by further comprising a supporting and limiting device (6), wherein the supporting and limiting device (6) comprises a sleeve (601), a support plate (602) is arranged at the top of the sleeve (601), the lower surface of the sleeve (601) is in contact with a glass slide (5), an immune composition (4) is arranged in the sleeve (601), a rod (1) penetrates through the support plate (602), the rod (1) is movably connected with the support plate (602), and a disc (2) is arranged in the sleeve (601).

3. An immunohistochemical antibody homogenizer according to claim 2, wherein: the improved spring support is characterized in that a boss (101) is arranged on the rod (1), the boss (101) is arranged in the sleeve (601), the boss (101) is attached to the lower surface of the support plate (602), a pressing cap (102) is connected to the upper end of the rod (1), a spring (103) is directly arranged on the pressing cap (102) and the support plate (602), the rod (1) is arranged in the spring (103), and the spring (103) is arranged above the support plate (602).

4. An immunohistochemical antibody homogenizer according to claim 3, wherein: the rod (1) is connected with the support plate (602) through threads.

5. An immunohistochemical antibody homogenizer according to claim 3, wherein: the novel screw rod is characterized in that a screw groove I (104) is arranged on the rod (1), a screw groove II (603) is arranged at the joint of the support plate (602) and the rod (1), the rod (1) is connected with the support plate (602) through a ball (604), and the ball (604) is arranged between the screw groove I (104) and the screw groove II (603).

6. An immunohistochemical antibody homogenizer according to claim 5, wherein: and the support plate (602) is provided with a mounting hole (605), the mounting hole (605) is communicated with the top of the spiral groove II (603) and the upper part of the support plate (602), and an opening at the upper end of the mounting hole (605) is connected with a jackscrew (606).

7. An immunohistochemical antibody homogenizer according to any one of claims 2-6, wherein: the support plate (602) is provided with a hole (607), and the hole (607) is communicated with the inside of the sleeve (601) and the upper part of the support plate (602).

8. An immunohistochemical antibody homogenizer according to any one of claims 2-6, wherein: a rubber gasket (7) is arranged between the sleeve (601) and the glass slide (5), the rubber gasket (7) is connected with the lower end of the sleeve (601), and the immune composition (4) is arranged in the rubber gasket (7).

9. An immunohistochemical antibody homogenizer according to any one of claims 2-6, wherein: two finger handrails (608) are arranged on the side of the support plate (602), and the finger handrails (608) extend to the outer side of the sleeve (601).