CN211133868U

CN211133868U - Oligonucleotide protecting group processor

Info

Publication number: CN211133868U
Application number: CN201921254916.4U
Authority: CN
Inventors: 邵永胜; 郑永华; 刘雷
Original assignee: Shanghai Generay Biotech Co ltd
Current assignee: Shanghai Generay Biotech Co ltd
Priority date: 2019-08-05
Filing date: 2019-08-05
Publication date: 2020-07-31
Anticipated expiration: 2029-08-05

Abstract

The utility model discloses an oligonucleotide protection group processor, this scheme is by the reaction chamber, and the cooperation of heating chamber constitutes, and wherein the heating chamber is filled with the oil layer for the heating along the lateral wall setting of reaction chamber in, heating chamber, and the heating intracavity still is provided with and is used for carrying out the heating element that heats to the oil layer. Compared with the prior art, the utility model has more accurate temperature control and more sufficient reaction; meanwhile, the consumption of water is greatly reduced, and the safety of sampling work after treatment is improved.

Description

Oligonucleotide protecting group processor

Technical Field

The utility model relates to an oligonucleotide processing technology, in particular to an oligonucleotide protecting group processing technology.

Background

The oligonucleotide is synthesized on the carrier by a solid phase method, the oligonucleotide needs to be cut off from the carrier after the synthesis is finished, the protecting group on the oligonucleotide chain is also removed in the cutting process, so that the subsequent purification work can be continued, and the current treatment method is to use an ammonolysis apparatus to finish the cutting and the removal of the protecting group.

The prior ammonolysis instrument has the following problems in the actual use process:

(1) the existing ammonolysis instrument measures the temperature in the whole cavity through the water temperature in the cavity, so that the measurement is not accurate;

(2) because the heating is by water heating, need add a large amount of water in the course of the work and maintain the temperature stable, the instrument gets into behind the liquid ammonia water can dissolve liquid ammonia, and the ammonolysis causes the splash when accomplishing uncapping.

SUMMERY OF THE UTILITY MODEL

Aiming at the problems of the existing ammonolysis apparatus in the using process, a new oligonucleotide protecting group processing scheme is needed.

Therefore, the utility model aims at providing an oligonucleotide protection group processor improves the accuracy of oligonucleotide protection group processing in-process temperature and avoids liquid splash from this.

In order to achieve the above object, the utility model provides an oligonucleotide protecting group processor, including reaction chamber, heating chamber, the heating chamber is filled with the oil layer for the heating along the lateral wall setting of reaction chamber in, the heating intracavity still is provided with and is used for carrying out the heating element that heats to the oil layer.

Further, the heating assembly comprises a heating pipe and an oil temperature gauge.

Further, an air inlet is arranged in the reaction cavity and connected with an air inlet control assembly.

Furthermore, the air inlet control assembly comprises an air inlet pipeline, an air outlet pipeline, an air inlet valve assembly, an air outlet valve assembly and a pressure transmitter, one end of the air inlet pipeline is connected with an air inlet on the reaction cavity, the other end of the air inlet pipeline is connected with an air source, and the air inlet valve assembly is arranged on the air inlet pipeline; one end of the exhaust pipeline is communicated with the air inlet pipeline, the other end of the exhaust pipeline is communicated with the exhaust port, and the exhaust valve assembly is arranged on the exhaust pipeline; the pressure transmitter is communicated with the air inlet pipeline.

Further, a cavity temperature detection assembly is further arranged in the reaction cavity.

Further, the treatment instrument further comprises a heat preservation layer, and the heat preservation layer is arranged along the outer side wall of the heating cavity.

Compared with the prior art, the utility model has more accurate temperature control and more sufficient reaction; meanwhile, the consumption of water is greatly reduced, and the safety of sampling work after treatment is improved.

Drawings

The invention is further described with reference to the following drawings and detailed description.

FIG. 1 is a schematic diagram of an oligonucleotide protecting group treatment apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic diagram showing the inside of an oligonucleotide protecting group treatment apparatus according to an embodiment of the present invention;

fig. 3 is a schematic diagram illustrating a configuration of an intake control assembly according to an embodiment of the present invention.

Detailed Description

In order to make the technical means, creation features, achievement purposes and functions of the present invention easy to understand and understand, the present invention is further explained by combining with the specific drawings.

Aiming at the defects of the existing oligonucleotide protecting group treatment scheme, the method abandons the existing water heating scheme and innovatively adopts an oil heating scheme, thereby not only improving the accuracy of temperature, but also greatly reducing the water for reaction and effectively avoiding liquid splashing.

Referring to FIGS. 1 and 2, there are shown diagrams illustrating the structure of the oligonucleotide protecting group treating apparatus given in this example. As can be seen, the oligonucleotide protecting group treating apparatus 100 is shown as an example, and comprises a housing 110, in which a corresponding reaction chamber 120 and a heating chamber 130 are disposed, and a corresponding chamber cover 140 is disposed on the top of the reaction chamber 120.

The housing 110 forms the infrastructure of the overall processor 100 for carrying the other components. The specific configuration of the box 110 may be determined according to actual requirements, and will not be described herein. By way of example, a square box 110 structure is shown.

The box body 110 is provided with some control components, such as an oil temperature controller, a cavity temperature controller, a pressure controller, a main switch, a heating controller, an air inlet controller, an air outlet controller, a power inlet wire and the like. Thereby serving to realize the basic functional operation of the oligonucleotide protecting group processor 100.

The middle part of the box body is provided with a corresponding reaction cavity 120 for carrying out the oligonucleotide protecting group reaction treatment.

The reaction chamber 120 is a spherical bottom cylinder, and is preferably made of stainless steel material, the inner diameter is preferably 180 mm and 250mm, and the height is preferably 150 mm and 200mm, so that the reaction chamber formed by the above steps can be pressurized and heated.

Further, a chamber cover 140 is disposed at a port on the top of the reaction chamber 120, and the chamber cover 140 is engaged with the port of the reaction chamber 120 and can seal the port of the reaction chamber 120 when closed, so that the reaction chamber 120 forms a sealed reaction chamber structure. The specific configuration of the cavity cover 140 can be determined according to actual requirements. By way of example, the chamber cover 140 is configured to fit with a top port of the reaction chamber 120, and the chamber cover 140 is mounted on the housing with respect to the reaction chamber 120 by a hinge structure, and a handle for facilitating the operation of the chamber cover is provided on the body of the chamber cover 140.

The reaction chamber 120 is provided with a chamber temperature detection assembly 122, which is connected with a chamber temperature controller arranged on the box body, and is used for detecting the temperature in the reaction chamber 120 in real time.

The chamber temperature detection assembly 122 is preferably constituted by a corresponding temperature sensor for acquiring temperature changes within the reaction chamber.

The reaction chamber 120 is further provided with a corresponding gas inlet 121 to be externally connected with a gas source. Whereby the gas inlet 121 is provided with a corresponding pressure valve and is connected to a source of ammonia gas. Thereby enabling ammonia gas and pressure to be added as the product reacts in the reaction chamber, depending on the process requirements.

The air inlet 121 is configured to be externally connected to an air supply via an air inlet control assembly 160. As shown in fig. 3, a schematic structural view of the intake air control assembly 160 employed in the present example is shown.

As can be seen, the intake control assembly 160 is mainly composed of an intake line 161, an exhaust line 162, an intake valve 163, an intake solenoid valve 164, an exhaust valve 165, an exhaust solenoid valve 166, and a pressure transformer 167, which are engaged with each other.

Wherein, the gas outlet end of the gas inlet pipeline 161 is communicated with the gas inlet 121 on the reaction chamber 120, and the gas inlet end of the gas inlet pipeline 161 is communicated with a gas source, such as a steel cylinder.

An intake valve 163 and an intake solenoid valve 164 are provided in the intake pipe 161 in this order in the direction from the intake end toward the exhaust end to control the opening and closing of the entire intake pipe.

The intake valve 163 may be manually operable to control the intake line open-closed state, while the intake solenoid valve 164 may be operable to automatically control the intake line open-closed state.

One end of the exhaust pipeline 162 is communicated with the air inlet pipeline 161 and is positioned between the air inlet electromagnetic valve 164 and the air outlet end of the air inlet pipeline 161; the other end of the exhaust duct 162 communicates with an exhaust port. Meanwhile, an exhaust solenoid valve 166 is provided in the exhaust line 162 to automatically control the open/close state of the exhaust line 162.

In order to further improve the reliability of the whole device, in this embodiment, a fast exhaust branch 168 is further added on the basis of the exhaust pipeline 162, the fast exhaust branch 168 is arranged in parallel with the exhaust pipeline 162, and one end of the fast exhaust branch 168 is communicated with the air inlet pipeline 161 and is positioned between the air inlet electromagnetic valve 164 and the air outlet end of the air inlet pipeline 161; the other end is also communicated with the exhaust port; meanwhile, an exhaust valve 165 is arranged on the quick exhaust branch 168 for manually and quickly controlling the opening and closing state of the quick exhaust branch 168, so that the safety and reliability of the whole scheme are ensured.

Further, the pressure transformer 167 is communicated with the intake line 161 through a line, and is located between the intake solenoid valve 164 and the outlet end of the intake line 161.

The pressure transformer 167 is used as a pressure detection device, can convert a pressure source into 0-20mA current to form output, can visually express pressure and control balance pressure, can take a signal when the pressure is over-pressurized, and reduces the pressure to play a role in control and protection.

The air inlet control assembly 160 constructed based on the scheme is integrally arranged in the box body 110, so that the whole treatment instrument is more compact in structure; meanwhile, the intake solenoid valve 164 is connected to an intake controller on the case, the exhaust solenoid valve 166 is connected to an exhaust controller on the case, and the pressure transformer 167 is connected to a pressure controller on the case.

On the basis of the above-mentioned reaction chamber 120 setting scheme, this example sets up a heating chamber 130 at the lateral wall of reaction chamber 120, and this heating chamber 130 sets up along the lateral wall of reaction chamber 120, from the whole reaction chamber 120 of outside cladding, and this heating chamber 130 intussuseption is filled with the conduction oil that is used for the heating simultaneously, realizes carrying out the bulk heating to the reaction chamber from the outside through the mode of oil heating, guarantees heating temperature's uniformity and accuracy nature.

An oil inlet 131 is formed in the top of the heating cavity 130, so that heat conduction oil can be injected into the heating cavity 130; meanwhile, an oil discharge valve 132 is arranged at the bottom of the heating cavity 130, and the oil layer in the heating cavity 130 is controlled by the cooperation of the two. The specific structure of the oil inlet 131 and the oil discharge valve 132 can be determined according to actual requirements, and will not be described herein.

On this basis, a heating assembly 133 is further disposed in the heating cavity 130 for adjusting the temperature of the oil in the heating cavity 130. The heating assembly 133 is controlled by a heating controller and an oil temperature controller on the tank.

The heating element 133 is preferably disposed at the bottom of the heating chamber 130, thereby ensuring that the heating tube does not burn empty under low oil conditions.

By way of example, the heating assembly 133 may be formed by combining a heating pipe and an oil temperature detector, wherein the heating pipe is used for heating the oil in the heating chamber 130 to adjust the oil temperature; and the oil temperature detector is used for detecting and displaying the oil temperature in the heating cavity 130 in real time so as to adjust the working state of the heating pipe.

The heating assembly 133 thus constructed can well control the reaction temperature in the reaction chamber 120, and at the same time, can timely adjust the reaction temperature in the reaction chamber 120 according to the production process. By way of example, the reaction temperature within the reaction chamber 120 may be maintained at 90 ℃ depending on the manufacturing process requirements.

In this embodiment, an insulating layer 150 is further disposed on an outer sidewall of the heating cavity 130 for insulating the heating cavity 130. The insulating layer 150 is disposed along the outer sidewall of the heating chamber 130, and covers the entire heating chamber 130 from the outside, thereby providing a good insulating effect for the heating chamber 130.

The insulating layer 150 is preferably made of an asbestos insulating layer, and can block the temperature of the heated heat-conducting oil in the heating chamber 130 from being conducted to the outside.

The oligonucleotide protecting group treatment instrument 100 provided in this example eliminates the conventional water heating mode to innovatively realize oil heating, thereby realizing precise control of the reaction temperature in the reaction chamber 120 to overcome the problems in the prior art.

On the basis, as a preferable scheme, the temperature control can be changed into cavity temperature control according to needs, and the accuracy of the temperature control is further improved.

Therefore, in the embodiment, a mode of synchronously detecting and controlling the oil temperature (the temperature of the heat conducting oil in the heating cavity 130) and the cavity temperature (the temperature in the reaction cavity 120) is adopted at the high-temperature end, and the heating is stopped as long as one of the oil temperature or the cavity temperature meets the condition, so that the over-temperature and the over-pressure can be effectively ensured. There are various methods for realizing the cavity temperature control, and those skilled in the art can select an appropriate method according to actual conditions to achieve the purpose, for example, temperature detection can be performed by a PID control mode, and then heating control is performed.

Meanwhile, in the scheme of the embodiment, only a small amount of water needs to be added into the reaction cavity, and only the original 1/10 is needed to finish the cutting and deprotection of the oligonucleotide, so that the original technical defects are greatly improved.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. Oligonucleotide protection group processor, its characterized in that, including reaction chamber, heating chamber, the heating chamber sets up along the lateral wall of reaction chamber, and it has the heating oil layer to fill in it, still be provided with in the heating chamber and be used for carrying out the heating element that heats to the oil layer, be provided with the air inlet in the reaction chamber, the air inlet control assembly that admits air is connected to the air inlet.

2. The oligonucleotide protecting group treater according to claim 1, wherein the heating unit includes a heating pipe and an oil thermometer.

3. The oligonucleotide protecting group processor according to claim 1, wherein the gas inlet control assembly comprises a gas inlet pipeline, a gas outlet pipeline, a gas inlet valve assembly, a gas outlet valve assembly and a pressure transmitter, one end of the gas inlet pipeline is connected with a gas inlet on the reaction chamber, the other end of the gas inlet pipeline is connected with a gas source, and the gas inlet valve assembly is arranged on the gas inlet pipeline; one end of the exhaust pipeline is communicated with the air inlet pipeline, the other end of the exhaust pipeline is communicated with the exhaust port, and the exhaust valve assembly is arranged on the exhaust pipeline; the pressure transmitter is communicated with the air inlet pipeline.

4. The oligonucleotide protecting group processor according to claim 1, wherein a chamber temperature detecting unit is further provided in the reaction chamber.

5. The oligonucleotide protecting group processor according to claim 1, further comprising a heat insulating layer disposed along an outer sidewall of the heating chamber.