CN211122704U

CN211122704U - Solid phase extraction column for purifying ginseng total saponin

Info

Publication number: CN211122704U
Application number: CN201922044791.9U
Authority: CN
Inventors: 郑凤家; 王政; 于连龙; 刘娜
Original assignee: Shandong Zhengsheng Health Management Service Co ltd
Current assignee: Zhiqi Pharmaceutical (Beijing) Co., Ltd
Priority date: 2019-11-25
Filing date: 2019-11-25
Publication date: 2020-07-28
Anticipated expiration: 2029-11-25

Abstract

In order to solve the problem that survey ginsenoside content process step is complicated, inefficiency, the utility model provides a solid-phase extraction post for ginsenoside purifies, a serial communication port, including the cylinder that is cylinder shell structure, can dismantle fixed connection in the cap of cylinder upper end and can dismantle the sealing washer of fixed connection in cylinder lower extreme, the inside top-down of cylinder is equipped with filler activation layer, pigment adsorbed layer and packing layer in proper order, the packing layer below still is equipped with the sieve the same with the cylinder internal diameter, filler activation layer is the mixed liquid of ethanol and pure water, the packing layer is macroporous resin, device simple structure, and convenient to use has greatly improved experimental efficiency.

Description

Solid phase extraction column for purifying ginseng total saponin

Technical Field

The utility model belongs to the technical field of solid phase extraction, especially, relate to a solid phase extraction post for ginsenoside purifies.

Background

The content of ginsenoside (such as ginsenoside Re, Rb1, Rb2, Rg, and Rf) is often determined in the process of health product inspection, Chinese medicinal material inspection, scientific research experiment, etc. The key step of accurately analyzing and determining the content of the total ginsenoside is to remove the interference of various pigments, saccharides, lipids and other substrates in health-care food and Chinese medicinal materials. At present, two methods for removing matrix interference exist, one is to purchase macroporous resin raw materials and manually fill an extraction column, and the method has the following defects: 1) experimenters are required to have higher skills, and the filling quality consistency is poorer; 2) consumes large physical strength, occupies most time and causes certain environmental pollution in the operation process. Another is chromatographic separation analysis, which has the following disadvantages: 1) matrix interference exists in the separation process, and complete separation is difficult to realize; 2) the separation time is 60min, the separation time is longer, and a large amount of organic solvent methanol is consumed, so that the environmental pollution is caused; 3) negative deviation exists when the total ginsenoside is measured. At present, the solid phase extraction column for measuring the total ginsenosides for experiments basically adopts the first method, and the experimental requirements of accurately, quickly and efficiently separating and measuring the total ginsenosides cannot be met.

Disclosure of Invention

In order to solve the problems, the utility model provides a solid phase extraction column for purification of ginseng total saponin, the technical scheme who adopts is as follows:

the utility model provides a solid-phase extraction post for ginsenoside purifies which characterized in that, including the cylinder that is cylinder shell structure, can dismantle the column cap of fixed connection in cylinder upper end and can dismantle the sealing washer of fixed connection in the cylinder lower extreme, the inside top-down of cylinder is equipped with filler activation layer, pigment adsorbed layer and packing layer in proper order, the packing layer below still is equipped with the sieve the same with the cylinder internal diameter, filler activation layer is the mixed liquid of ethanol and pure water, the packing layer is macroporous resin.

Preferably, the pigment adsorption layer is neutral alumina powder.

Preferably, the inner diameter of the column body is 2-3 cm, and the length of the column body is 6-8 cm.

The beneficial effects of the utility model reside in that:

1. the ethanol solution is added above the filler layer, so that substances in the filler activation layer can be prevented from volatilizing when the solid-phase extraction column is not used, and the filler layer can be activated when the materials are extracted;

2. the pigment adsorption layer can effectively remove pigment impurities in the matrix to be extracted, and the sieve plate prevents internal materials from flowing out;

3. the solid phase extraction column is prefabricated in advance, the experimental efficiency and the accuracy of analysis and test are improved, and meanwhile, the labor and the time are saved.

Drawings

FIG. 1 is a schematic view of the structure of the present invention

Wherein, the device comprises 1-column cap, 2-column, 3-filler activating layer, 4-pigment adsorbing layer, 5-filler layer, 6-sieve plate and 7-sealing ring.

Detailed Description

Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience of description of the present invention, and do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

As shown in figure 1 for total saponins of ginseng purification of solid phase extraction column, mainly including the cylinder 2, the column cap 1, the seal ring 7, the cylinder 2 is upper and lower uncovered hollow cylindrical shell, the inside diameter is 4cm, the main body 2 upper end through the column cap 1 seal, the lower extreme through the seal ring 3 seal, in the cylinder 2 inside is equipped with from top to bottom distribution filler activation layer 3, pigment adsorption layer 4 packing layer 5 and sieve 6, wherein filler activation layer 3 is 70% ethanol and pure water mixture, pigment adsorption layer 4 is neutral alumina powder, packing layer 5 is D101 macroporous resin, sieve 6 is fixed connection in the packing layer 5 below, sieve 6 is equipped with a plurality of micropores, can pass through liquid and dissolved in the liquid material, but can not pass through macroporous resin and neutral alumina powder, prevent the material from flowing out, influence the accuracy of the experimental result, when the extraction column is not used, the filler activation layer 3 can play a sealing role on the filler layer 5 to prevent the volatilization of internal substances, and in the experimental process, the filler activation layer 3 plays an activating role on the filler layer 3.

The solid phase extraction column of the device can be prepared in advance for standby, when the ginseng total saponin purification test is carried out, the column cap 1 and the sealing ring 3 are firstly opened, the filler activating layer 3 flows through the filler layer 3 and then flows out from the lower part of the column body 2, then a sample to be extracted is poured from the upper end opening of the column body 2, after impurities such as pigment in the sample are adsorbed by the pigment adsorbing layer 4, the extraction of the ginseng total saponin is continuously carried out through the filler layer 5, and finally liquid flows out from the lower end outlet of the column body 2, so that the extraction process is completed.

The device has the advantages of simple structure, convenient manufacture and convenient storage, greatly improves the experimental efficiency and saves the labor and time.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. The utility model provides a solid-phase extraction post for ginsenoside purifies which characterized in that, including the cylinder that is cylinder shell structure, can dismantle the column cap of fixed connection in cylinder upper end and can dismantle the sealing washer of fixed connection in the cylinder lower extreme, the inside top-down of cylinder is equipped with filler activation layer, pigment adsorbed layer and packing layer in proper order, the packing layer below still is equipped with the sieve the same with the cylinder internal diameter, filler activation layer is the mixed liquid of ethanol and pure water, the packing layer is macroporous resin.

2. The solid phase extraction column for purification of ginsenoside as in claim 1, wherein the pigment adsorbing layer is neutral alumina powder.

3. The solid phase extraction column for purification of ginsenoside as in claim 1 or 2, wherein the inner diameter of the column is 2-3 cm, and the length of the column is 6-8 cm.