CN211086318U - Multi-channel magnetic separation, mixing and unloading integrated mechanism for immunodetection - Google Patents
Multi-channel magnetic separation, mixing and unloading integrated mechanism for immunodetection Download PDFInfo
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- CN211086318U CN211086318U CN201921974507.1U CN201921974507U CN211086318U CN 211086318 U CN211086318 U CN 211086318U CN 201921974507 U CN201921974507 U CN 201921974507U CN 211086318 U CN211086318 U CN 211086318U
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Abstract
The utility model relates to a chemiluminescence detection area, concretely relates to a multichannel magnetism separation mixing uninstallation integration mechanism for immunodetection, including main lead screw motor and the rail block connecting block that links to each other with it, the rail block connecting block cup joints on linear guide, the rail block connecting block is connected with bar magnet motor guide rail installing support, be fixed with bar magnet lead screw motor on the bar magnet motor guide rail installing support, be connected with bar magnet installing block on the bar magnet motor guide rail installing support, the bar magnet installing block is connected with a plurality of bar magnets, bar magnet motor guide rail installing support bottom has the loading head that is used for loading disposable magnetism separation cover, several pain points that conventional magnetism particle immunodetection was difficult to solve have been accomplished with simplest mode to this application, there is extremely strong practicality. The POCT system has the advantages of high cost performance, small volume and no liquid path, can be applied to POCT products, and has a large expansion space.
Description
Technical Field
The application relates to the field of chemiluminescence detection, in particular to a multichannel magnetic separation, mixing and unloading integrated mechanism for immunodetection.
Background
The magnetic particle-based immunoassay system is immunoassay, and has become a mainstream methodology of clinical immunoassay due to the advantages of easy automation, high reaction speed, high signal-to-noise ratio and the like, and has extremely wide application in hospitals at all levels. In the instrument, the magnetic separation cleaning mechanism has the greatest influence on the performance of the instrument, so the quality of the mechanism directly influences the core performance of the whole machine. Conventional magnetic particle chemiluminescence all is large-scale instrument, when carrying out the magnetic separation, carries out the magnetic bead through loading the permanent magnet in the reaction cup outside and adsorbs, adsorbs the back, siphons away the waste liquid through the imbibition needle, reinjects the washing liquid afterwards to ensure the magnetic bead through mixing mechanism and suspend again, repeated thrice can be accomplished.
The existing cleaning mechanism is very complex and huge, needs a special liquid path system and a blending mechanism, and relates to various elements such as a peristaltic pump, a plunger pump, an electromagnetic valve, a blending motor, an eccentric mechanism, a lifting mechanism, cleaning liquid management, waste liquid management and the like, so that the existing cleaning mechanism is very complex in instrument, high in cost, high in failure rate and high in maintenance cost.
Disclosure of Invention
In order to solve the problems in the prior art, a multi-channel magnetic separation, mixing and unloading integrated mechanism for magnetic particle immunodetection is provided, which greatly reduces the complexity of a magnetic separation cleaning mechanism, improves the reliability of the magnetic separation mechanism and reduces the cost of the magnetic separation mechanism.
In order to achieve the technical effects, the specific scheme of the application is as follows:
the utility model provides a multichannel magnetic separation mixing uninstallation integration mechanism for immunodetection which characterized in that: including main lead screw motor and the guide rail slider connecting block that links to each other with it, guide rail slider connecting block cup joints on linear guide, guide rail slider connecting block is connected with bar magnet motor guide rail installing support, be fixed with bar magnet lead screw motor on the bar magnet motor guide rail installing support, be connected with bar magnet installing block on the bar magnet motor guide rail installing support, bar magnet installing block is connected with a plurality of bar magnets, bar magnet motor guide rail installing support bottom has the loading head that is used for loading disposable magnetic separation cover, the loading head loads through frictional force, once wash magnetic separation cover and can load one or more.
Further, the main screw rod motor is connected with the guide rail sliding block through a T-shaped screw rod nut.
Furthermore, the linear guide rail is positioned on a main motor guide rail mounting bracket, and the main lead screw motor is mounted on the main motor guide rail mounting bracket.
Furthermore, the bar magnet installation block is sleeved with at least one optical axis guide rail, and the bottom of the bar magnet installation block is connected with a plurality of bar magnets.
Further, the number of the magnetic rods is 1 to 96.
Furthermore, a reflection-type photoelectric detection switch for detecting whether the disposable magnetic separation sleeve is normally loaded or unloaded is arranged at the bottom of the main motor guide rail mounting bracket, and the reflection-type photoelectric detection switch is arranged beside the disposable magnetic separation sleeve.
The application has the advantages that:
1. by introducing the magnetic rod and the disposable magnetic separation sleeve, the passive magnetic separation is changed into active magnetic separation, so that the separation speed is greatly increased, and meanwhile, the separation efficiency is further improved by multi-channel parallel separation; meanwhile, the reaction liquid can be uniformly mixed and the cleaning liquid can be resuspended by the reciprocating motion of the magnetic separation sleeve, the structure is simple, the reliability is high, and no liquid splashes; whether the magnetic separation sleeve is loaded or not is automatically detected through the array reflection-type photoelectric switch, so that the reliability of the instrument is improved, and the use cost is reduced; the separation of the magnetic separation sleeve is directly completed through the magnetic bar without an additional mechanism. Therefore, the assembly completes several pain points which are most difficult to solve by the conventional magnetic particle immunoassay in the simplest mode, and has extremely strong practicability. The POCT system has the advantages of high cost performance, small volume and no liquid path, can be applied to POCT products, and has a large expansion space.
2. This application utilizes the mode of bar magnet and disposable magnetic separation cover to change passive magnetic separation into active magnetic separation, has simplified the process of magnetic separation by a wide margin, because the magnetic field is closer apart from the magnetic particle to can do reciprocal magnetism of inhaling, consequently separation efficiency is higher, and the separation time is shorter, is far superior to passive magnetic separation mode.
3. The magnetic separation device and the magnetic separation method realize multi-channel parallel magnetic separation, and greatly improve the magnetic separation efficiency.
4. The automatic detection of the magnetic separation sleeve is realized, and the wrong result and the waste of corresponding reagents caused by the fact that the magnetic separation sleeve is not loaded are avoided.
5. This application can accomplish the mixing of reaction liquid and the resuspension of washing liquid through up-and-down motion, and simple structure, mixing are efficient, can not take place the liquid that eccentric mixing probably leads to and splash.
6. The magnetic rod motor unloading device can directly unload the disposable magnetic separation sleeve through the magnetic rod motor, does not need an additional mechanism, and is simple in structure, reliable and durable.
Drawings
Fig. 1 is a schematic structural diagram of the present application.
In the drawings: the method comprises the following steps of 1-main lead screw motor, 2-main motor guide rail mounting bracket, 3-T-shaped lead screw nut, 4-guide rail slider connecting block, 5-linear guide rail, 6-reflection type photoelectric detection switch, 7-disposable magnetic separation sleeve, 8-magnetic bar lead screw motor, 9-magnetic bar motor guide rail mounting bracket, 10-magnetic bar mounting block, 11-optical axis guide rail and 12-magnetic bar.
Detailed Description
Example 1
As shown in fig. 1, a multichannel magnetic separation mixing uninstallation integration mechanism for immunodetection includes main lead screw motor 1 and guide rail slider connecting block 4 that links to each other with it, guide rail slider connecting block 4 cup joints on linear guide 5, guide rail slider connecting block 4 is connected with bar magnet motor guide rail installing support 9, be fixed with bar magnet lead screw motor 8 on the bar magnet motor guide rail installing support 9, be connected with bar magnet installing block 10 on the bar magnet motor guide rail installing support 9, bar magnet installing block 10 is connected with a plurality of bar magnets 12, bar magnet motor guide rail installing support 9 bottom has the loading head that is used for loading disposable magnetic separation cover 7, the loading head loads through frictional force, once wash the magnetic separation cover and can load one or more. According to the magnetic separation device, the magnetic rod 12 and the disposable magnetic separation sleeve 7 are introduced, passive magnetic separation is changed into active magnetic separation, the separation speed is greatly improved, and meanwhile, the separation efficiency is further improved through multi-channel parallel separation; meanwhile, the reaction liquid can be uniformly mixed and the cleaning liquid can be resuspended by the reciprocating motion of the magnetic separation sleeve, the structure is simple, the reliability is high, and no liquid splashes; whether the magnetic separation sleeve is loaded or not is automatically detected through the array reflection-type photoelectric switch, so that the reliability of the instrument is improved, and the use cost is reduced; the separation of the magnetic separation sleeve is directly accomplished by the magnetic bar 12 without the need for an additional mechanism. Therefore, the assembly completes several pain points which are most difficult to solve by the conventional magnetic particle immunoassay in the simplest mode, and has extremely strong practicability. The POCT system has the advantages of high cost performance, small volume and no liquid path, can be applied to POCT products, and has a large expansion space.
Example 2
The utility model provides a multichannel magnetic separation mixing uninstallation integration mechanism for immunodetection includes main lead screw motor 1 and the guide rail slider connecting block 4 that links to each other with it, guide rail slider connecting block 4 cup joints on linear guide 5, guide rail slider connecting block 4 is connected with bar magnet motor guide rail installing support 9, be fixed with bar magnet lead screw motor 8 on the bar magnet motor guide rail installing support 9, be connected with bar magnet installing block 10 on the bar magnet motor guide rail installing support 9, bar magnet installing block 10 is connected with a plurality of bar magnets 12, bar magnet motor guide rail installing support 9 bottom has the loading head that is used for loading disposable magnetic separation cover 7, the loading head loads through frictional force, once wash the magnetic separation cover and can load one or more.
The main screw motor 1 is connected with the guide rail slide block through a T-shaped screw nut 3. The linear guide rail 5 is positioned on the main motor guide rail mounting bracket 2, and the main screw rod motor 1 is mounted on the main motor guide rail mounting bracket 2. The bar magnet installation block 10 is sleeved with at least one optical axis guide rail 11, and the bottom of the bar magnet installation block 10 is connected with a plurality of bar magnets 12. The number of the magnetic rods 12 is 1 to 96. The bottom of the main motor guide rail mounting bracket 2 is provided with a reflection type photoelectric detection switch 6 for detecting whether the disposable magnetic separation sleeve 7 is normally loaded or unloaded, and the reflection type photoelectric detection switch 6 is arranged beside the disposable separation magnetic sleeve.
When magnetic separation is needed, the main lead screw motor 1 rotates, the guide rail slider connecting block 4 drives the magnetic bar 12 motor mounting bracket to move downwards, the magnetic bar 12 motor mounting bracket is provided with a loading head of a magnetic separation sleeve, the loading head is in interference fit with the magnetic separation sleeve, the magnetic separation sleeve is loaded through friction force, the main lead screw motor 1 descends to a fixed height to complete loading of the magnetic separation sleeve, after loading is completed, the main lead screw motor 1 ascends to the highest position, meanwhile, whether the disposable magnetic separation sleeve 7 is successfully loaded is detected through a reflection type photoelectric detection sensor, each channel corresponds to a reflection photoelectric switch, after all the magnetic separation sleeves are confirmed to be completely loaded, the magnetic bar lead screw motor 8 starts to rotate to drive the magnetic bar mounting block 10 to move downwards, when the magnetic bar 12 just reaches the bottom of the magnetic separation sleeve, the rotation is stopped, then, the main lead screw motor 1 starts to rotate to drive the magnetic bar 12 assembly to move downwards, when the magnetic separation sleeve contacts liquid containing magnetic particles, the magnetic separation sleeve slowly descends to the bottom of the magnetic particle liquid, so that the magnetic particles are gradually captured to the end part of the magnetic separation sleeve, the magnetic particles can be adsorbed and separated fully through multiple times of slow ascending and descending within 10-30 s, after the adsorption is completed, the main lead screw motor 1 ascends to the topmost end, the magnetic particles are all adsorbed to the end part of the outer wall of the magnetic separation sleeve, the separated liquid is pumped away and injected with new cleaning liquid again, at the moment, the magnetic particles need to be re-suspended in the cleaning liquid again, the main lead screw motor 1 moves downwards again until the magnetic separation sleeve enters the cleaning liquid, then, the magnetic rod lead screw motor 8 starts to move upwards, the magnetic field disappears, the magnetic particles slowly separate from the end part of the magnetic separation sleeve, and in order to accelerate the process, the main lead screw motor 1 can do reciprocating motion up and down at the frequency of 5-20 Hz, ensuring the magnetic particles and the cleaning liquid to be fully mixed and suspended, raising the main screw rod motor 1 to the highest position again after the completion of the magnetic particle and cleaning liquid mixing, completing one magnetic separation, cleaning and mixing, and repeating the actions if multiple times of magnetic separation and cleaning are needed; in addition, the mechanism can also be used for transferring the magnetic particles into a reagent for uniformly reacting, or separating the magnetic particles in a reaction solution into a cleaning solution, or transferring the magnetic particles into a substrate solution for reacting and completing luminescence detection. After all separation actions are finished, when the magnetic separation sleeves are unloaded, the magnetic rod screw motor 8 moves downwards to drive the magnetic rod 12 to move downwards, and when the magnetic rod 12 reaches the bottom of the magnetic separation sleeves, the magnetic rod 12 continues to move downwards until all the magnetic separation sleeves are ejected by the magnetic rod 12 and are separated from the magnetic rod 12 mounting plate.
According to the magnetic separation device, the magnetic rod 12 and the disposable magnetic separation sleeve 7 are introduced, passive magnetic separation is changed into active magnetic separation, the separation speed is greatly improved, and meanwhile, the separation efficiency is further improved through multi-channel parallel separation; meanwhile, the reaction liquid can be uniformly mixed and the cleaning liquid can be resuspended by the reciprocating motion of the magnetic separation sleeve, the structure is simple, the reliability is high, and no liquid splashes; whether the magnetic separation sleeve is loaded or not is automatically detected through the array reflection-type photoelectric switch, so that the reliability of the instrument is improved, and the use cost is reduced; the separation of the magnetic separation sleeve is directly accomplished by the magnetic bar 12 without the need for an additional mechanism. Therefore, the assembly completes several pain points which are most difficult to solve by the conventional magnetic particle immunoassay in the simplest mode, and has extremely strong practicability. The POCT system has the advantages of high cost performance, small volume and no liquid path, can be applied to POCT products, and has a large expansion space.
This application utilizes bar magnet 12 and disposable magnetic separation cover 7's mode to change passive form's magnetic separation into active magnetic separation, has simplified the process of magnetic separation by a wide margin, because the magnetic field is closer apart from the magnetic particle to can do reciprocal magnetism of inhaling, consequently separation efficiency is higher, and the separation time is shorter, far superior to passive form magnetic separation. The magnetic separation device and the magnetic separation method realize multi-channel parallel magnetic separation, and greatly improve the magnetic separation efficiency. The automatic detection of the magnetic separation sleeve is realized, and the wrong result and the waste of corresponding reagents caused by the fact that the magnetic separation sleeve is not loaded are avoided. This application can accomplish the mixing of reaction liquid and the resuspension of washing liquid through up-and-down motion, and simple structure, mixing are efficient, can not take place the liquid that eccentric mixing probably leads to and splash. The disposable magnetic separation sleeve 7 can be directly unloaded through the magnetic bar 12 motor, an additional mechanism is not needed, and the structure is simple, reliable and durable.
Claims (6)
1. The utility model provides a multichannel magnetic separation mixing uninstallation integration mechanism for immunodetection which characterized in that: the magnetic separation device comprises a main lead screw motor (1) and a guide rail sliding block connecting block (4) connected with the main lead screw motor, wherein the guide rail sliding block connecting block (4) is sleeved on a linear guide rail (5), the guide rail sliding block connecting block (4) is connected with a magnetic bar motor guide rail mounting support (9), a magnetic bar lead screw motor (8) is fixed on the magnetic bar motor guide rail mounting support (9), a magnetic bar mounting block (10) is connected on the magnetic bar motor guide rail mounting support (9), the magnetic bar mounting block (10) is connected with a plurality of magnetic bars (12), and a loading head used for loading a disposable magnetic separation sleeve (7) is arranged at the bottom of the magnetic bar motor guide rail mounting support (9).
2. The multichannel magnetic separation, blending and unloading integrated mechanism for immunodetection according to claim 1, wherein the multichannel magnetic separation, blending and unloading integrated mechanism comprises: the main screw rod motor (1) is connected with the guide rail sliding block through a T-shaped screw rod nut (3).
3. The multichannel magnetic separation, blending and unloading integrated mechanism for immunodetection according to claim 1, wherein the multichannel magnetic separation, blending and unloading integrated mechanism comprises: the linear guide rail (5) is positioned on the main motor guide rail mounting bracket (2), and the main lead screw motor (1) is mounted on the main motor guide rail mounting bracket (2).
4. The multichannel magnetic separation, blending and unloading integrated mechanism for immunodetection according to claim 1, wherein the multichannel magnetic separation, blending and unloading integrated mechanism comprises: the magnetic bar mounting block (10) is sleeved with at least one optical axis guide rail (11), and the bottom of the magnetic bar mounting block (10) is connected with a plurality of magnetic bars (12).
5. The multichannel magnetic separation, blending and unloading integrated mechanism for immunodetection according to claim 4, wherein the multichannel magnetic separation, blending and unloading integrated mechanism comprises: the number of the magnetic rods (12) is 1-96.
6. The multichannel magnetic separation, blending and unloading integrated mechanism for immunodetection according to claim 1, wherein the multichannel magnetic separation, blending and unloading integrated mechanism comprises: the bottom of the main motor guide rail mounting bracket (2) is provided with a reflection type photoelectric detection switch (6) for detecting whether the disposable magnetic separation sleeve (7) is normally loaded or unloaded, and the reflection type photoelectric detection switch (6) is arranged beside the disposable separation magnetic sleeve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921974507.1U CN211086318U (en) | 2019-11-15 | 2019-11-15 | Multi-channel magnetic separation, mixing and unloading integrated mechanism for immunodetection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921974507.1U CN211086318U (en) | 2019-11-15 | 2019-11-15 | Multi-channel magnetic separation, mixing and unloading integrated mechanism for immunodetection |
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| CN211086318U true CN211086318U (en) | 2020-07-24 |
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| CN201921974507.1U Active CN211086318U (en) | 2019-11-15 | 2019-11-15 | Multi-channel magnetic separation, mixing and unloading integrated mechanism for immunodetection |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113820195A (en) * | 2021-11-25 | 2021-12-21 | 成都宜乐芯生物科技有限公司 | Multichannel parallel pretreatment device |
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- 2019-11-15 CN CN201921974507.1U patent/CN211086318U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113820195A (en) * | 2021-11-25 | 2021-12-21 | 成都宜乐芯生物科技有限公司 | Multichannel parallel pretreatment device |
| CN113820195B (en) * | 2021-11-25 | 2022-03-08 | 成都宜乐芯生物科技有限公司 | Multichannel parallel pretreatment device |
| EP4187250A1 (en) * | 2021-11-25 | 2023-05-31 | Chengdu Illumaxbio Technology Co., Ltd. | Multi-channel parallel pretreatment device |
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