CN211086314U - Kit for rapidly detecting vomitoxin - Google Patents
Kit for rapidly detecting vomitoxin Download PDFInfo
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- CN211086314U CN211086314U CN201921076524.3U CN201921076524U CN211086314U CN 211086314 U CN211086314 U CN 211086314U CN 201921076524 U CN201921076524 U CN 201921076524U CN 211086314 U CN211086314 U CN 211086314U
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Abstract
The utility model discloses a kit for short-term test vomitoxin, the kit comprises gold mark micropore reagent and detection card, include as follows: the gold-labeled microporous reagent is prepared by freeze-drying colloidal gold-labeled vomitoxin monoclonal antibody at-50-10 ℃; the detection card consists of a card shell and a test strip, and the test strip is arranged in the card shell; the test strip consists of a bottom plate, a nitrocellulose membrane, a sample pad and a water absorption pad. The gold-labeled microporous reagent in the kit is prepared by freeze drying, so that the biological activity of the gold-labeled microporous reagent can be maximally preserved, and the vomitoxin in a sample can be fully reacted with the colloidal gold-labeled vomitoxin monoclonal antibody in the detection process, so that the detection sensitivity is improved, and the kit has the advantages of simplicity and rapidness in operation, low cost and the like.
Description
Technical Field
The utility model relates to detect vomitoxin's immunological technical field, in particular to detect vomitoxin's kit fast.
Background
Vomitoxin, also called deoxynivalenol, is trichothecene toxin mainly produced by fusarium graminearum and fusarium roseum, is colorless acicular crystal, has a melting point of 151-153 ℃, is soluble in water and polar solvents such as aqueous methanol, aqueous ethanol or ethyl acetate and the like, has strong heat resistance and acid resistance, and can be stored in ethyl acetate for a long time. Vomitoxin is one of the most common mycotoxins and widely exists in grains such as rice, corn, wheat, soybean and the like. The contamination of cereal grains with vomitoxin has been reported worldwide, particularly in china, japan, the united states, argentina and south africa. In the high-incidence areas of malignant tumors such as gastric cancer, esophageal cancer and the like in China, vomitoxin is one of main pollution mycotoxins in the diet of residents. Scientific research results show that the toxic action of vomitoxin mainly shows emetic toxicity, cytotoxicity, immunosuppression, teratogenicity and possibly genetic toxicity.
At present, the detection method of vomitoxin comprises a physicochemical detection method and an immunological detection method, wherein the main thin-layer chromatography (T L C), the high-performance liquid chromatography (HP L C), the Gas Chromatography (GC) and the T L C methods are complex in sample purification, need to use standard toxin and easily cause environmental pollution, the HP L C method and the GC method need to use expensive detection instruments, are high in professional degree and difficult to popularize and apply in a basic level, and enzyme-linked immunosorbent assay (E L ISA) in the immunological detection method has the defects of multiple required reagents, complex operation steps, time consumption, complex environmental interference factors and the like, and is low in sensitivity.
Aiming at the defects of the existing vomitoxin detection technology, the method which has high detection sensitivity, simple and convenient operation, high speed, is suitable for detecting the vomitoxin on site is very important, and has very important significance for protecting the food safety.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a detection sensitivity is high, and is easy and simple to handle, quick, and is applicable to the on-the-spot detection vomitoxin's method.
Therefore, the utility model adopts the following technical proposal,
a kit for rapidly detecting vomitoxin, which consists of a gold-labeled micropore reagent and a detection card, and comprises the following components:
the gold-labeled microporous reagent is prepared by freeze-drying colloidal gold-labeled vomitoxin monoclonal antibody at-50-10 ℃; the detection card consists of a card shell and a test strip, and the test strip is arranged in the card shell; the test strip consists of a bottom plate, a nitrocellulose membrane, a sample pad and a water absorption pad; the nitrocellulose membrane is arranged in the middle of the bottom plate, one end of the nitrocellulose membrane is covered by the sample pad, and the other end of the nitrocellulose membrane is covered by the absorbent pad.
Preferably, the length of one end of the nitrocellulose membrane covered by the sample pad is millimeter; the other end of the nitrocellulose membrane is covered by the absorbent pad for a length of a millimeter.
Preferably, one end of the nitrocellulose membrane, which is close to the sample pad, is provided with a detection line; and one end of the nitrocellulose membrane, which is close to the absorption pad, is provided with a quality control line.
Preferably, the detection line is coated with vomitoxin coupling antigen, and the quality control line is coated with anti-mouse secondary antibody.
Preferably, the anti-mouse secondary antibody is a goat anti-mouse IgG antibody or a rabbit anti-mouse IgG antibody.
Preferably, the gold-labeled micropore reagent is contained in a micropore, and the micropore is sealed and stored in a gold-labeled micropore reagent barrel.
Preferably, the number of the detection cards is 10, and the number of the micropores is 10.
The utility model provides a detect kit of vomiting poison fast, gold mark micropore reagent is prepared at-50 ℃ -10 ℃ freeze-drying by the vomiting toxin monoclonal antibody of colloidal gold mark in this kit and obtains, can not only preserve the biological activity of the vomiting toxin monoclonal antibody of colloidal gold mark with the maximize, and can make the vomiting toxin in the sample fully react with the vomiting toxin monoclonal antibody of colloidal gold mark in the testing process, thereby the sensitivity of detection has been improved, and the operation is simple and convenient quick, advantages such as with low costs, this kit is applicable to the witnessed inspections of vomiting toxin simultaneously.
Drawings
Fig. 1 is a structural diagram of the test strip of the present invention.
Fig. 2 is a top view of the test strip of the present invention.
Detailed Description
In order to make the objects, features and advantages of the present invention clearer, embodiments of the present invention are described in more detail below with reference to the accompanying drawings and examples, and in the following description, many specific details are set forth to provide a thorough understanding of the present invention, but the present invention can be implemented in many other ways than those described. Therefore, the present invention is not limited by the specific embodiments disclosed below.
A kit for rapidly detecting vomitoxin, which consists of a gold-labeled micropore reagent and a detection card, and comprises the following components:
the gold-labeled microporous reagent is prepared by freeze-drying colloidal gold-labeled vomitoxin monoclonal antibody at-50-10 ℃; the detection card consists of a card shell and a test strip, and the test strip is arranged in the card shell; the test strip consists of a bottom plate 1, a nitrocellulose membrane 2, a sample pad 3 and a water absorption pad 4; the nitrocellulose membrane 2 is arranged in the middle of the bottom plate 1, one end of the nitrocellulose membrane 2 is covered by the sample pad 3, and the other end of the nitrocellulose membrane 2 is covered by the absorbent pad 4.
Wherein the length of one end of the nitrocellulose membrane 2 covered by the sample pad 3 is 1 mm; the other end of the nitrocellulose membrane 2 is covered by the absorbent pad 4 to a length of 2 mm.
Wherein, one end of the nitrocellulose membrane 2 close to the sample pad 3 is provided with a detection line 5; one end of the nitrocellulose membrane 2 close to the absorption pad 3 is provided with a quality control line 6.
Wherein, vomitoxin coupling antigen is coated on the detection line 5, and anti-mouse secondary antibody is coated on the quality control line 6.
Wherein the anti-mouse secondary antibody is a goat anti-mouse IgG antibody or a rabbit anti-mouse IgG antibody.
Wherein, gold mark micropore reagent holds in the micropore, micropore seal keeps in gold mark micropore reagent bucket.
Wherein, the number of detection card is 10, the number of micropore is 10.
The use method of the kit for rapidly detecting the vomitoxin comprises the following steps: and adding a sample to be detected into the micropores containing the gold-labeled micropore reagent, uniformly mixing until the gold-labeled micropore reagent is fully dissolved, and specifically combining vomitoxin in the sample to be detected with the colloidal gold-labeled vomitoxin monoclonal antibody if the sample to be detected contains the vomitoxin. All liquid in the micropores is taken out and dripped onto the sample pad 3, when the concentration of the vomitoxin in the sample to be detected is lower than the detection limit or zero, the colloidal gold-labeled vomitoxin monoclonal antibody can be combined with the vomitoxin coupling antigen coated on the detection line 5 in the chromatography process, and red strips appear on the detection line 5 and the quality control line 6; if the concentration of the vomitoxin in the sample to be detected is equal to or higher than the detection limit, the colloidal gold-labeled vomitoxin monoclonal antibody is completely combined with the vomitoxin, so that the vomitoxin monoclonal antibody cannot be retained on the detection line 5, and a red strip does not appear at the detection line 5.
The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (7)
1. The kit for rapidly detecting vomitoxin is characterized by consisting of a gold-labeled micropore reagent and a detection card, and comprises the following components: the gold-labeled microporous reagent is prepared by freeze-drying colloidal gold-labeled vomitoxin monoclonal antibody at-50-10 ℃; the detection card consists of a card shell and a test strip, and the test strip is arranged in the card shell; the test strip consists of a bottom plate, a nitrocellulose membrane, a sample pad and a water absorption pad; the nitrocellulose membrane is arranged in the middle of the bottom plate, one end of the nitrocellulose membrane is covered by the sample pad, and the other end of the nitrocellulose membrane is covered by the absorbent pad.
2. The kit for rapidly detecting vomitoxin according to claim 1, wherein one end of the nitrocellulose membrane covered by the sample pad has a length of 1 mm; the other end of the nitrocellulose membrane covered by the absorbent pad had a length of 2 mm.
3. The kit for rapidly detecting vomitoxin according to claim 1, wherein a detection line is arranged at one end of the nitrocellulose membrane close to the sample pad; and one end of the nitrocellulose membrane, which is close to the water absorption pad, is provided with a quality control line.
4. The kit for rapidly detecting vomitoxin according to claim 3, wherein the detection line is coated with vomitoxin coupling antigen, and the quality control line is coated with anti-mouse secondary antibody.
5. The kit for rapidly detecting vomitoxin according to claim 4, wherein the anti-mouse secondary antibody is goat anti-mouse IgG antibody or rabbit anti-mouse IgG antibody.
6. The kit for rapidly detecting vomitoxin according to claim 1, wherein the gold-labeled micropore reagent is contained in a micropore, and the micropore is hermetically stored in a gold-labeled micropore reagent barrel.
7. The kit for rapidly detecting vomitoxin according to claim 1 or 6, wherein the number of said detection cards is 10, and the number of said wells is 10.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921076524.3U CN211086314U (en) | 2019-07-10 | 2019-07-10 | Kit for rapidly detecting vomitoxin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921076524.3U CN211086314U (en) | 2019-07-10 | 2019-07-10 | Kit for rapidly detecting vomitoxin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211086314U true CN211086314U (en) | 2020-07-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921076524.3U Active CN211086314U (en) | 2019-07-10 | 2019-07-10 | Kit for rapidly detecting vomitoxin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211086314U (en) |
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2019
- 2019-07-10 CN CN201921076524.3U patent/CN211086314U/en active Active
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