CN211043111U - Device for detecting nitrite in sugar - Google Patents
Device for detecting nitrite in sugar Download PDFInfo
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- CN211043111U CN211043111U CN201921233966.4U CN201921233966U CN211043111U CN 211043111 U CN211043111 U CN 211043111U CN 201921233966 U CN201921233966 U CN 201921233966U CN 211043111 U CN211043111 U CN 211043111U
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Abstract
A nitrite detection device in sugar for detecting nitrite content in sugar, the nitrite detection device includes: the colorimetric tube with a plug is used for containing sugar liquid to be detected; a reagent pack comprising a first reagent pack and a second reagent pack for containing a reagent for detecting nitrite; the vortex instrument is used for uniformly mixing the sugar solution to be detected in the colorimetric tube with the plug with the reagent; and a detector, comprising: the device comprises a detection unit, a display unit, an initialization unit and a control unit, wherein the detection unit, the display unit and the initialization unit are electrically connected with the control unit; the detection unit is used for detecting the absorbance value of the sugar solution to be detected after being uniformly mixed with the reagent and transmitting the absorbance value to the control unit; the initialization unit is used for setting a standard curve of the stored nitrite; and the control unit compares the received absorbance value with the nitrite standard curve and outputs a detection result to the display unit.
Description
Technical Field
The utility model relates to a nitrite detection device in sugar belongs to food detection technology field.
Background
Nitrite has been recognized as a safety hazard in some food products as a preservative and color former. Sugar is not only a sweet food which is directly eaten by people in daily life, but also an indispensable important raw material for food, beverage and pharmaceutical industry, and has great social demand.
With the enhancement of the safety awareness of people's food, sugar enterprises pay more attention to the content of nitrite in sugar, the content of nitrite becomes one of important indexes for sugar safety detection, and national standard GB 5009.33-2016 specifies a method for measuring the content of nitrite and nitrate in food, but the method for measuring nitrite and nitrate in national standard is complicated. At present, some manufacturers develop a test strip for rapidly detecting nitrite, and react with the test strip to develop color, compare a color comparison card and semi-quantitatively detect the nitrite; patent document CN 108827956 a introduces a reagent for detecting nitrite in salted vegetables and a detection method thereof, and prepares a reagent for detecting nitrite in salted vegetables, and judges whether the content of nitrite in the salted vegetables exceeds the standard according to whether the reaction between the juice of the salted vegetables and the nitrite reagent is purple red or not. The colorimetric method visually judges the result, and has the advantages of more influencing factors, large error of the result and low accuracy.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that lie in to prior art not enough, provide a nitrite detection device in sugar, through providing one kind including utensil stopper colour comparison tube, two kinds of reagent packages, nitrite detection device in the sugar of vortex appearance and detector, can the on-the-spot test sugaring trade finished product sugar and the nitrite content in the in-process, and the reaction is quick, the reading is accurate, can take nitrite control technology according to the field test result in the sugaring production technology, prepare higher quality sugar product.
The utility model discloses the technical problem that solve is realized through following technical scheme:
a nitrite detection device in sugar for detecting nitrite content in sugar, the nitrite detection device includes: the colorimetric tube with a plug is used for containing sugar liquid to be detected; a reagent pack comprising a first reagent pack and a second reagent pack for containing a reagent for detecting nitrite; the vortex instrument is used for uniformly mixing the sugar solution to be detected in the colorimetric tube with the plug with the reagent; and a detector, comprising: the device comprises a detection unit, a display unit, an initialization unit and a control unit, wherein the detection unit, the display unit and the initialization unit are electrically connected with the control unit; the detection unit is used for detecting the absorbance value of the sugar solution to be detected after being uniformly mixed with the reagent and transmitting the absorbance value to the control unit; the initialization unit is used for setting a standard curve of the stored nitrite; and the control unit compares the received absorbance value with the nitrite standard curve and outputs a detection result to the display unit.
In order to prevent the sugar solution to be detected from spilling and leaking, the colorimetric tube with a plug is a colorimetric tube with a screw cap, and the capacity of the colorimetric tube with a plug is 10 ml.
Preferably, the first reagent package is filled with a first reagent, and the first reagent comprises 92 wt% -96 wt% of tartaric acid and 4 wt% -8 wt% of sulfanilamide.
Preferably, the mass of the first reagent is 0.2g to 0.3 g.
Preferably, the second agent comprises 2 wt% to 4 wt% of naphthyl ethylenediamine hydrochloride and 96 wt% to 98 wt% of tartaric acid.
Preferably, the second agent has a mass of 0.1g to 0.2 g.
Preferably, the vortex apparatus has a shaking frequency of greater than 250 times/min.
To sum up, the utility model discloses a nitrite detection device in the sugar including utensil stopper colour comparison tube, two kinds of reagent package, vortex appearance and detector can the on-the-spot detection system sugar trade finished product sugar and the nitrite content in the in-process, and the reaction is quick, and the reading is accurate, can take nitrite control technology according to the on-the-spot test result in the system sugar production technology, prepares higher quality sugar product.
The technical solution of the present invention will be described in detail with reference to the accompanying drawings and specific embodiments.
Drawings
FIG. 1 is a schematic view of the device for detecting nitrite in sugar according to the present invention;
fig. 2 is a schematic view of the detector of the present invention.
Detailed Description
All of the starting materials of the present invention, without particular limitation as to their source, may be purchased commercially or prepared according to conventional methods well known to those skilled in the art. Unless otherwise stated, all reagents used in the present invention are analytically pure.
FIG. 1 is a schematic view of the device for detecting nitrite in sugar according to the present invention; fig. 2 is a schematic view of the detector of the present invention. As shown in fig. 1 and fig. 2, in the present invention, the device for detecting nitrite in sugar comprises a cuvette 100 with a plug, a reagent pack 200, a vortex apparatus 300 and a detector 400.
The colorimetric tube with a plug 100 is used for containing sugar solution to be measured, and in order to prevent the sugar solution to be measured from spilling and leaking, the colorimetric tube with a plug 100 with a screw cap is preferred, and the capacity of the colorimetric tube with a plug is preferably 10 ml.
The reagent pack 200 is used to contain reagents for detecting nitrite.
The reagent pack 200 comprises a first reagent pack and a second reagent pack, wherein the first reagent pack is filled with a first reagent, and the first reagent comprises 92 wt% (mass percent) to 96 wt% of tartaric acid and 4 wt% to 8 wt% of sulfanilamide. The second reagent package is filled with a second reagent, the second reagent is used for performing coupled color reaction, and the second reagent comprises 2-4 wt% of naphthyl ethylenediamine hydrochloride and 96-98 wt% of tartaric acid. In the present invention, the mass of the first reagent is preferably 0.2g to 0.3g, and the mass of the second reagent is preferably 0.1g to 0.2 g.
The tartaric acid provides an acidic medium, and the nitrite is diazotized under the acidic medium condition.
In the traditional detection method using one reagent pack, the density of different components of the reagent is different, so that the reagent is not easy to be uniformly mixed, and the problem of reduced test precision is caused. The utility model discloses in, through setting up two reagent packages, install different reagents respectively, can mix branch mixed reagent, shorten color reaction time, make the absorbance value can reach stably fast, show the controllability that has improved the reaction.
The vortex apparatus 300 is used for uniformly mixing the sugar solution to be detected in the colorimetric tube with plug 100 with the reagent. Preferably, the vortex apparatus 300 has a shaking frequency of greater than 250 times/min.
The detector 400 includes a detection unit, a display unit 420, a correction unit 430, an initialization unit 440, and a control unit 450, and the detection unit, the display unit, the correction unit, and the initialization unit are electrically connected to the control unit. It should be added that the detecting apparatus 400 further includes common modules such as a power supply unit 460, which are not described herein again.
The detection unit comprises a light source 411, a detection port 412 and a photometer 413, and is used for detecting the absorbance value of the sugar solution to be detected after being uniformly mixed with the reagent and transmitting the absorbance value to the control unit, wherein the detection port 412 is used for placing the stopper colorimetric tube 100 containing the developed sugar solution to be detected. It should be added that the detection of the absorbance value by the photometer is prior art and will not be described herein, and those skilled in the art can select different types of light sources and photometers according to actual needs.
The initialization unit is used for setting a standard curve of the storage nitrite. The nitrite standard curve is a curve showing the relationship between nitrite concentration and absorbance value, and can be obtained in various ways. For example, because the nitrite concentration and the absorbance value are in a linear relationship, firstly, preparing nitrite standard solutions with different concentrations (for example, refer to national standard GB 5009.33-2016), taking 10ml of the nitrite standard solutions into a color comparison tube 100 with a plug, adding a first reagent in a first reagent bag, then placing the mixture into a vortex instrument 300, shaking for 2min-6min, and then standing for 2min-4 min; and adding the second reagent in the second reagent bag, placing the second reagent bag in a vortex apparatus 300, shaking for 30s, and standing for 10-15 min. The absorbance values of the developed nitrite standard solution of different concentrations are measured by the detector 400, a nitrite standard curve corresponding to the nitrite concentration is made according to the recorded absorbance values, and the nitrite standard curve is stored in the initialization unit.
It should be noted that the nitrite standard curve may be set before the detector 400 leaves the factory, and may be directly called after leaving the factory; or can be set by the user when in use.
After receiving the absorbance value, the control unit compares the absorbance value with the nitrite standard curve, and outputs a detection result (such as the absorbance value, the nitrite concentration, the nitrite content and the like) to the display unit.
The display unit is used for displaying information such as detection results (such as absorbance values, nitrite concentrations, nitrite content and the like) and system parameters.
The utility model discloses a use as follows:
first, a nitrite standard curve is set in the initialization unit, and if the nitrite standard curve is already stored in the initialization unit, the user can directly proceed to the next step.
Weighing 5g of sample (such as sugar to be detected, product in preparation and the like) and dissolving the sample in ultrapure water, and fixing the volume to a 100m L volumetric flask to prepare the sugar solution to be detected, wherein the volume of the sample to be detected is generally 5g to 100ml when the sugar solution to be detected is prepared, and the sample to be detected needs to be diluted if the sugar solution exceeds the detection limit.
Taking 10ml of sugar solution to be detected in the colorimetric tube with a plug 100; adding a first reagent in a first reagent bag into the sugar liquid to be detected, placing the sugar liquid in the vortex instrument 300, shaking for 2-6 min, and then standing for 2-4 min; and adding the second reagent in the second reagent bag, placing the second reagent bag in a vortex apparatus 300, shaking for 30s, and standing for 10-15 min. And (5) placing the developed sugar liquid to be detected in a detector 400 to obtain the concentration of nitrite in the sugar liquid to be detected.
If the sample is diluted, the content of nitrite in the sample is as follows:
the utility model discloses a detector 400 combines reagent package 200, nitrite content in can accurate measurement sugar, and the reaction is quick, and the reading is accurate, is applicable to the on-the-spot test of sugar industry finished product sugar and in-process nitrite content, can take nitrite control technology according to the on-the-spot test result in the sugar manufacturing production technology, prepares higher quality sugar product.
The present invention will be further described with reference to the following specific examples.
Example one
In this embodiment, adopt the utility model discloses a nitrite detection device detects nitrite content in beet white sugar in the sugar, for verifying the utility model discloses nitrite detection device's in the sugar degree of accuracy and precision will carry out the contrast experiment with national standard method (GB 5009.33-2016 food nitrite and nitrate's determine method, spectrophotometry).
In the embodiment, 5g of sugar beet sugar is weighed and dissolved in ultrapure water, the volume is determined to 100m L volumetric flask, a sugar solution to be detected is prepared, 10ml of sugar solution to be detected is taken and placed in the colorimetric tube with a plug 100, a first reagent in a first reagent bag is added into the sugar solution to be detected, the sugar solution is placed in the vortex instrument 300 and shaken for 3min, then the sugar solution is placed and stood for 2min, a second reagent in a second reagent bag is added, the sugar solution to be detected is placed in the vortex instrument 300 and shaken for 30s, then the sugar solution is placed for 10min after color development, the nitrite concentration in the sugar solution to be detected is obtained, the nitrite concentration is converted into the nitrite content in the sugar beet sugar, and the detection is repeated for three times, and the experimental results are shown in table 1 below.
TABLE 1
The result shows that the device for detecting nitrite in sugar has stable detection result on nitrite in beet and white sugar and good parallelism; compare with the national standard method that adopts spectrophotometer, the result is unanimous basically, shows that the utility model discloses nitrite detection device's in the sugar detection data is accurate, and precision is high, can be quick, the nitrite content in the accurate detection sugar.
Example two
In this embodiment, get beet sugar manufacturing in-process, adopt the utility model discloses nitrite detection device detects sugar manufacturing technology in-process nitrite content in sugar. The beet sugar product comprises: extracting juice, filtering syrup, and concentrating syrup.
In this embodiment, 5g of homogenate (extract, filtered syrup, concentrated syrup) is weighed and dissolved in ultrapure water, the volume is fixed to a 250m L volumetric flask, a sugar solution to be measured is prepared, 10ml of sugar solution to be measured is taken and placed in the colorimetric tube with a plug 100, a first reagent in a first reagent pack is added to the sugar solution to be measured, the sugar solution is placed in the vortex apparatus 300 and shaken for 6min, then the sugar solution is kept stand for 2min, a second reagent in a second reagent pack is added, the sugar solution to be measured is placed in the vortex apparatus 300 and shaken for 30s, then the sugar solution is kept stand for 10min, the sugar solution to be measured after color development is placed in the detector 400, the concentration of nitrite in the sugar solution to be measured is obtained, and is converted into the content of nitrite in the product, and the experimental results are.
TABLE 2
The result shows that the device for detecting nitrite in sugar can detect the content of nitrite in sugar products.
EXAMPLE III
In this embodiment, the nitrite content in five different finished sugar products (#1, #2, #3, #4, #5) is detected by the nitrite detection device of the present invention, and the nitrite content in the five finished sugar products (#1, #2, #3, #4, #5) is detected by the detection method of the existing laboratory, since the nitrite content in some of the finished sugar products is very low, and the measurement results of the nitrite detection device in sugar and the UV-visible spectrophotometer may have a large deviation when the nitrite content is very low, the finished sugar products are diluted to 10g/100m L and 20g/100m L respectively during the test, thereby reducing the error caused by the measurement,
the existing detection method of the laboratory comprises the steps of sucking a 10m L sample to be detected, putting the sample into a 10m L colorimetric tube, adding a nitrite detection reagent, shaking uniformly, standing for 10min, putting the sample into an ultraviolet-visible spectrophotometer for reading to obtain an absorbance value, and obtaining the nitrite content through conversion.
Nitrite content (mg/Kg) — absorbance value
If the sample is diluted, the true nitrite content is:
nitrite content (mg/Kg) — absorbance value × dilution.
The results of the experiments are shown in table 3 below.
TABLE 3
In the table, the absorbance value is the testing result of current laboratory detection method, and the nitrite calculated value is the numerical value after converting the absorbance value into nitrite content, and nitrite detection device is for utilizing in the sugar the utility model discloses nitrite detection device's testing result in the sugar.
As can be seen from the above table, the measuring value of the device for detecting nitrite in sugar of the utility model is basically stable and can be controlled within the error range; the measurement value of the detection method of the existing laboratory is also stable, but the existing calculation method has problems, so that the calculation result is about 1.3 times of the real result. If the subjectivity of the unified correction is strong, the measured data can not be objectively measured through actual tests, and the subjectivity calibration needs to be carried out according to operation experience.
Example four
In this embodiment, through the utility model discloses a nitrite detection device in sugar and current laboratory detection method detect the sodium nitrite standard solution of different concentrations respectively. The detection method of the existing laboratory is the same as that of the embodiment, and is not described herein again.
Preparing sodium nitrate standard solution (0.1 mg/L, 0.2 mg/L, 0.4 mg/L, 0.5 mg/L and 1 mg/L), and respectively detecting by using the device for detecting nitrite in sugar and the existing laboratory detection method, wherein the experimental results are shown in the following table 4.
TABLE 4
In the table, the absorbance value is the detection result of the existing laboratory detection method, and the calculated value is the value obtained by converting the absorbance value into the nitrite content; error (1) is the error of the detection result of the existing laboratory detection method; nitrite detection device is for utilizing in the sugar the utility model discloses nitrite detection device's in the sugar testing result, error (2) are for utilizing the utility model discloses nitrite detection device's in the sugar error of testing result.
The result shows that the detection value of the device for detecting nitrite in sugar of the utility model basically accords with the true value and can be controlled within the error range; in the existing laboratory detection method, although the detection value is relatively stable, the existing calculation method has problems, so that the calculation result is 1.3 times of the real result, and the deviation is large.
To sum up, the utility model discloses a nitrite detection device in the sugar including utensil stopper colour comparison tube, two kinds of reagent package, vortex appearance and detector can the on-the-spot detection system sugar trade finished product sugar and the nitrite content in the in-process, and the reaction is quick, and the reading is accurate, can take nitrite control technology according to the on-the-spot test result in the system sugar production technology, prepares higher quality sugar product.
Claims (3)
1. A nitrite detection device in sugar for detect nitrite content in sugar, characterized in that, nitrite detection device includes:
the colorimetric tube with a plug is used for containing sugar liquid to be detected;
a reagent pack comprising a first reagent pack and a second reagent pack for containing a reagent for detecting nitrite;
the vortex instrument is used for uniformly mixing the sugar solution to be detected in the colorimetric tube with the plug with the reagent; and
a detector, comprising: the device comprises a detection unit, a display unit, an initialization unit and a control unit, wherein the detection unit, the display unit and the initialization unit are electrically connected with the control unit;
the detection unit is used for detecting the absorbance value of the sugar solution to be detected after being uniformly mixed with the reagent and transmitting the absorbance value to the control unit; the initialization unit is used for setting a standard curve of the stored nitrite; and the control unit compares the received absorbance value with the nitrite standard curve and outputs a detection result to the display unit.
2. The device for detecting nitrite in sugar as claimed in claim 1, wherein the cuvettes are cuvettes with screw caps and have a capacity of 10 ml.
3. The device for detecting nitrite in sugar as claimed in claim 1, wherein the frequency of shaking of the vortex apparatus is more than 250 times/min.
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| CN201921233966.4U CN211043111U (en) | 2019-07-31 | 2019-07-31 | Device for detecting nitrite in sugar |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921233966.4U CN211043111U (en) | 2019-07-31 | 2019-07-31 | Device for detecting nitrite in sugar |
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| CN211043111U true CN211043111U (en) | 2020-07-17 |
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