CN211035891U

CN211035891U - PCR application of sample box

Info

Publication number: CN211035891U
Application number: CN201921818022.3U
Authority: CN
Inventors: 杨榆玲
Original assignee: Kunming Medical University
Current assignee: Kunming Medical University
Priority date: 2019-10-28
Filing date: 2019-10-28
Publication date: 2020-07-17
Anticipated expiration: 2029-10-28

Abstract

The utility model discloses a PCR sample adding box, which comprises a box body, wherein the box body comprises a storage area and a reaction area, the storage area is positioned at the rear end of the reaction area, and the storage area is connected with the reaction area through a hinge; the storage area includes first box body, the inside of first box body is equipped with the heat preservation, the preceding lateral wall fixedly connected with refrigeration box of heat preservation, a plurality of cavitys are cut apart into by the baffle in the inside of heat preservation, the lower lateral wall of a plurality of cavitys all is equipped with the recess, sliding connection has the slider in the recess, fixedly connected with frame on the slider, the container has been placed in the frame, the front end of frame is equipped with the baffle, the lower extreme of baffle rotates and is connected with the pull ring, the lower extreme of pull ring is equipped with the card, the utility model discloses a link and ice bag guarantee that the cooling effect practices thrift the space simultaneously, convenient storage and transport, and simple to use, reaction household utensils are placed to the reaction area, and the cryopreservation makes.

Description

PCR application of sample box

Technical Field

The utility model relates to a biological assay equipment technical field, concrete field is a PCR adds appearance box.

Background

In the PCR (polymerase chain reaction), DNA is denatured at 95 ℃ in vitro to become single-stranded, and at low temperature (usually about 60 ℃) the primer and single-stranded are combined according to the principle of base complementary pairing, and then the temperature is adjusted to the optimum reaction temperature (about 72 ℃) of the DNA polymerase, and the DNA polymerase synthesizes a complementary strand along the direction from phosphate to pentose (5 '-3'). The PCR instrument manufactured based on polymerase is actually a temperature control device, and can be well controlled among denaturation temperature, renaturation temperature and extension temperature. The PCR is utilized to carry out a biological reaction experiment, the current common method for amplifying target DNA is required to add sample into a test tube in advance before PCR reaction, and partial materials are required to be kept at low temperature in the sample adding process, so that the influence on the experimental accuracy caused by the characteristic change of the materials due to the temperature rise is prevented.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a PCR adds appearance box to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the present invention provides a PCR sample adding box, comprising a box body, wherein the box body comprises a storage region and a reaction region, the storage region is located at the rear end of the reaction region, and the storage region and the reaction region are connected by a hinge;

the storage area comprises a first box body, a heat insulation layer is arranged in the first box body, a refrigeration box is fixedly connected to the front side wall of the heat insulation layer, the heat insulation layer is divided into a plurality of cavities by partition plates, grooves are formed in the lower side walls of the cavities, sliding blocks are connected in the grooves in a sliding mode, a frame is fixedly connected to the sliding blocks, a container is placed in the frame, a baffle plate is arranged at the front end of the frame, the lower end of the baffle plate is rotatably connected with a pull ring, a clamping piece is arranged at the lower end of the pull ring, a clamping groove is formed in the partition plate below the baffle plate, and the clamping groove is matched;

the reaction zone comprises a second box body, a heat preservation layer is arranged inside the second box body, a refrigeration box is fixedly connected to the upper side wall of the heat preservation layer, a reagent rack is arranged above the refrigeration box, an opening is formed in the left side wall and the right side wall of the second box body, the reagent rack is slidably clamped in the opening, a first cover plate is hinged to the upper end of the left side of the second box body, a first boss is arranged at the right end of the first cover plate, a second cover plate is hinged to the upper end of the right side of the second box body, a second boss is arranged at the left end of the second cover plate, the first boss is matched with the second boss in a clamped mode, a pressing plate is hinged to the front end and the rear end of the second box body, and the pressing plate is located above the second cover plate.

Preferably, an ice bag is placed in the refrigeration box.

Preferably, the upper end of the first box body is provided with a buckle, the front end of the second box body is provided with a clamping lock, and the clamping lock is matched with the buckle.

Preferably, the rear side wall of the first box body is provided with a support, and the lower side wall of the second box body is hinged with a support leg.

Preferably, a handle is arranged at the upper end of the first box body.

Preferably, the reagent racks are of different formats.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model provides a PCR adds appearance box, links through the hinge and guarantees that the cooling effect practices thrift the space simultaneously with the ice bag, convenient storage and transport, and is simple to use, and the reaction household utensils are placed to the reaction zone, and the low temperature is preserved, and convenient allotment, additives such as storage area storage biological enzyme, reaction zone and storage area are placed perpendicularly, conveniently draw materials from the storage area and directly add to the reaction zone household utensils in, save time, convenient control guarantees that the reagent is in the low temperature state at the transfer process, reduces contaminated possibility.

Drawings

Fig. 1 is a schematic structural view of the present invention;

fig. 2 is a top view of the present invention;

fig. 3 is a left side view of the present invention;

fig. 4 is an enlarged view of a portion a of fig. 3 according to the present invention.

In the figure: 1-box body, 2-storage area, 201-first box body, 202-clamping groove, 203-clapboard, 204-cavity, 205-groove, 206-slide block, 207-frame, 208-container, 209-baffle, 210-pull ring, 211-card, 3-reaction area, 301-second box body, 302-reagent rack, 303-notch, 304-first cover plate, 305-second cover plate, 306-first boss, 307-second boss, 308-pressing plate, 4-heat preservation layer, 5-refrigeration box, 6-ice bag, 7-buckle, 8-buckle, 9-bracket, 10-support leg and 11-handle.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution: a PCR sample adding box is shown in figure 1 and comprises a box body 1, wherein the box body 1 comprises a storage area 2 and a reaction area 3, the storage area 2 is used for storing additives, the reaction area 3 is used for placing samples and adding the additives to the samples, the storage area 2 is positioned at the rear end of the reaction area 3, the storage area 2 and the reaction area 3 are connected through a hinge, the storage area 2 is vertically arranged during use, the reaction area 3 is horizontally arranged, and the samples are conveniently sampled from the storage area 2 and added to the reaction area 3;

the storage area 2 comprises a first box body 201, the first box body 201 is a shell and used for protecting and supporting the storage area 2, a heat insulation layer 4 is arranged inside the first box body 201, the heat insulation layer 4 keeps a low-temperature environment in the storage area 2 and meets the storage requirement of additives, a refrigeration box 5 is fixedly connected to the front side wall of the heat insulation layer 4, the refrigeration box 5 is a box body for containing refrigerants and has excellent heat conductivity, the heat insulation layer 4 is divided into a plurality of cavities 204 by partition plates 203, the storage area 2 is divided into a plurality of cavities 204 by the partition plates 203, the cavities 204 are used for containing additives, grooves 205 are formed in the lower side walls of the cavities 204, sliding blocks 206 are connected in the grooves 205 in a sliding mode, frames 207 are fixedly connected to the sliding blocks 206, the frames 207 are fixed to the sliding blocks 206, the movement of the frames 207 is realized through the sliding of the sliding blocks 206 in the grooves 205, and, the container 208 is placed in the frame 207, the container 208 is used for storing additives, the types of the additives in the storage area 2 can be changed by changing the container 208, the storage area 2 is adjusted to adapt to different use requirements, the front end of the frame 207 is provided with a baffle 209, the baffle 209 protects the container 208 and prevents foreign matters from entering the additives in the damage container 208 or the pollution container 08, the lower end of the baffle 209 is rotatably connected with a pull ring 210, as shown in fig. 4, the lower end of the pull ring 210 is provided with a card 211, a baffle 203 below the baffle 209 is provided with a card slot 202, the card slot 202 is in clamping fit with the card 211, as shown in fig. 4, when the baffle 209 is closed, the pull ring 210 is in a vertical state, the card 211 is clamped in the card slot 202, the baffle 209 is kept in a closed state, the baffle 209 is prevented from being opened, the foreign matters enter the additives in the damage container, the card 211 is kept horizontal with the pull ring 210, the shutter 209 can be opened, the pull ring 210 is pulled, the frame 207 is taken out by the sliding of the slider 206 in the groove 205, and the additive in the container 208 can be operated;

the reaction zone 3 comprises a second box body 301, as shown in fig. 2, a heat-insulating layer 4 is arranged in the second box body 301, the heat-insulating layer 4 keeps a low-temperature environment in the reaction zone 3 and meets the temperature requirement when a sample is added, a refrigeration box 5 is fixedly connected to the upper side wall of the heat-insulating layer 4, the refrigeration box 5 is a box body for containing a refrigerant and has excellent heat conductivity, a reagent rack 302 is arranged above the refrigeration box 5, the reagent rack 302 is positioned above the refrigeration box 5, the distance is short, the refrigeration effect is good, openings 303 are formed in the left side wall and the right side wall of the second box body 301, the openings 303 facilitate the installation and adjustment of the reagent rack 302, the reagent rack 302 is slidably clamped in the openings 303, the reagent rack 302 is convenient to adjust the distribution and the quantity in the reaction zone 3 to adapt to the requirements of different experiments, a first cover plate 304 is hinged to the upper end of the left, a first boss 306 is provided at the right end of the first cover plate 304, a second cover plate 305 is hinged to the right upper end of the second container 301, the left end of the second cover plate 305 is provided with a second boss 307, the first boss 306 and the second boss 307 are matched and clamped, when the reaction zone is closed, the first cover plate 304 and the second cover plate 305 are matched and sealed with the reaction zone 3 through the clamping of the first boss 306 and the second boss 307, the contact between the reaction zone 3 and the outside is isolated, the cleanness and the sanitation of the reaction zone 3 are kept, the front and rear ends of the second box 301 are hinged with press plates 308, the press plates 308 are positioned above the second cover plate 305, after the first cover plate 304 and the second cover plate 305 are snap-fitted into the closed reaction zone 3 by the first boss 306 and the second boss 307, the pressing plate 308 presses the second cover plate 305 to prevent the first cover plate 304 and the second cover plate 305 from being opened and sealed untight, which results in environmental pollution of the reaction zone 3.

Specifically, as shown in fig. 3, the ice bag 6 is placed in the refrigeration box 5, so that the refrigeration effect is guaranteed, refrigeration can be kept by replacing the ice bag 6, and meanwhile, the ice bag 6 can be repeatedly used, so that the replacement is convenient, and the cost is reduced.

Specifically, as shown in fig. 3, the upper end of the first box 201 is provided with a buckle 7, the front end of the second box 301 is provided with a latch 8, and the latch 8 is matched with the buckle 7, so that the first box 201 and the second box 301 are stably connected, and the box 1 is convenient to transfer and carry.

Specifically, as shown in fig. 3, a support 9 is disposed on a rear side wall of the first container 201, the support 9 enables the first container to be held in a stable vertical state, a lower side wall of the second container 301 is hinged to a support leg 10, the support leg 10 stably supports the second container 301, the second container 301 is kept in a horizontal state, and the support leg 10 and the support 9 are mutually matched to keep the container 1 in a stable open state, so that a person can conveniently operate sample adding.

Specifically, as shown in fig. 1, a handle 11 is disposed at the upper end of the first box 201, which is convenient for transferring and carrying.

Specifically, as shown in fig. 2, the reagent rack 302 has different specifications, and is convenient for holding reagent tubes of different specifications, and can add samples for multiple sets of experiments simultaneously.

The working principle is as follows: the drawings of the utility model are views of the expanded box body, the box body 1 is carried to a storage chamber, the clamping lock 8 and the buckle 7 are released to open the box body 1, the support leg 10 and the bracket 9 are mutually matched to support the box body 1 to keep a stable open state, the operation of personnel is convenient, the sanitation in the box body 1 is checked, the ice bag 6 in the refrigeration box 5 is replaced to ensure the low-temperature environment, the pull ring 210 is flattened, the card 211 keeps a horizontal state along with the pull ring 210, the baffle 209 is pulled out, the sliding of the slider 206 in the groove 205 brings the frame 207 out, the container 208 containing different additives is replaced to meet the experimental requirements, the baffle 209 is pushed back afterwards, the pull ring 210 is erected to ensure the card 211 to be clamped in the clamping groove 202, then the box body 1 is closed, the first cover plate 304 and the second cover plate 305 are opened after the press plate 308 is opened, the ice bag 6 in the refrigeration box 5 is also replaced to ensure the low, prepare closed first apron 304 and second apron 305 after accomplishing, close clamp plate 308, closed box body 1, fastening kayser 8 and buckle 7, transfer box body 1 to the laboratory bench through handle 11, later release kayser 8 and buckle 7, open box body 1, keep the stable open mode of box body 1, later to opening second box body 301, put the sample on reagent frame 302, operate first box body 201 afterwards, select suitable additive to add the allotment in proper order, carry out the experiment test to the sample after adding the allotment, and close box body 1, accomplish the application of sample.

In the description of the present invention, unless expressly stated or limited otherwise, the terms "mounted," "connected," and "fixed" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral part; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The utility model discloses the standard part that uses all can purchase from the market, and dysmorphism piece all can be customized according to the description with the record of drawing of description, and the concrete connection mode of each part all adopts conventional means such as ripe bolt, rivet, welding among the prior art, and machinery, part and equipment all adopt prior art, and conventional model, including circuit connection adopts conventional connection mode among the prior art, does not detailed here again.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A PCR sample adding box comprises a box body (1), and is characterized in that: the box body (1) comprises a storage area (2) and a reaction area (3), the storage area (2) is positioned at the rear end of the reaction area (3), and the storage area (2) is connected with the reaction area (3) through a hinge;

the storage area (2) comprises a first box body (201), a heat-insulating layer (4) is arranged inside the first box body (201), the front side wall of the heat-insulating layer (4) is fixedly connected with a refrigeration box (5), the interior of the heat-insulating layer (4) is divided into a plurality of cavities (204) by partition plates (203), grooves (205) are formed in the lower side walls of the cavities (204), sliding blocks (206) are connected in the grooves (205) in a sliding mode, a frame (207) is fixedly connected on the sliding block (206), a container (208) is placed in the frame (207), a baffle plate (209) is arranged at the front end of the frame (207), the lower end of the baffle plate (209) is rotatably connected with a pull ring (210), a clamping piece (211) is arranged at the lower end of the pull ring (210), a clamping groove (202) is arranged on the partition plate (203) below the baffle plate (209), and the clamping groove (202) is in clamping fit with the clamping piece (211);

the reaction zone (3) comprises a second box body (301), a heat-insulating layer (4) is arranged inside the second box body (301), a refrigeration box (5) is fixedly connected to the upper side wall of the heat-insulating layer (4), a reagent rack (302) is arranged above the refrigeration box (5), a notch (303) is formed in the left side wall and the right side wall of the second box body (301), the reagent rack (302) is slidably clamped in the notch (303), a first cover plate (304) is hinged to the upper left side end of the second box body (301), a first boss (306) is arranged at the right end of the first cover plate (304), a second cover plate (305) is hinged to the upper right side end of the second box body (301), a second boss (307) is arranged at the left end of the second cover plate (305), the first boss (306) is matched and clamped with the second boss (307), and pressing plates (308) are hinged to the front end and the back end of the second box body (301), the pressure plate (308) is located above the second cover plate (305).

2. A PCR loading cassette according to claim 1, wherein: an ice bag (6) is placed in the refrigeration box (5).

3. A PCR loading cassette according to claim 1, wherein: the upper end of first box body (201) is equipped with buckle (7), the front end of second box body (301) is equipped with kayser (8), kayser (8) with buckle (7) cooperate.

4. A PCR loading cassette according to claim 1, wherein: the rear side wall of the first box body (201) is provided with a support (9), and the lower side wall of the second box body (301) is hinged with a support leg (10).

5. The PCR cartridge according to claim 4, wherein: the upper end of the first box body (201) is provided with a handle (11).

6. A PCR loading cassette according to claim 1, wherein: the reagent racks (302) have different specifications.