CN211014833U - Device for regulating and controlling environmental humidity of sample on fluorescence imaging system microscope - Google Patents
Device for regulating and controlling environmental humidity of sample on fluorescence imaging system microscope Download PDFInfo
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- CN211014833U CN211014833U CN201922212153.3U CN201922212153U CN211014833U CN 211014833 U CN211014833 U CN 211014833U CN 201922212153 U CN201922212153 U CN 201922212153U CN 211014833 U CN211014833 U CN 211014833U
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Abstract
The utility model discloses a device of regulation and control sample place environmental humidity on fluorescence imaging system microscope, the device main part is a closed box, wherein include slide glass plummer A, small-size electron hygrometer place recess B, the ultrapure water recess C of splendid attire, box apron D, with pipe E, the outlet duct F that box and external gas circuit are connected. During the test, place small-size electron hygrometer in recess B, pour into recess C with ultrapure water into, be connected to the nitrogen gas steel bottle with air duct E, open the nitrogen gas valve, place the sample, cover the apron and adjust nitrogen gas velocity of flow and can realize the collection of fluorescence life-span imaging test and spectrum to the sample under specific humidity environment.
Description
Technical Field
The utility model relates to an auxiliary device that microscope observed, the dynamic change's of sample under high humidity environment auxiliary observation device is observed to normal position specifically says so.
Background
The existing fluorescence imaging system can only realize the optical microscope observation, the fluorescence lifetime imaging and the spectrum acquisition of a specific sample under the conditions of room temperature and air. However, if the in-situ observation of the continuous change of the sample in the high humidity environment is to be realized, the in-situ continuous observation of the sample in the high humidity environment is more helpful to thoroughly understand the change conditions of various physicochemical characteristics of the sample in the dynamic chemical environment, and great help is generated for scientific research work.
SUMMERY OF THE UTILITY MODEL
In order to solve the problem that the prior art lacks an observation method which can realize real-time in-situ observation of a sample under a specific gas atmosphere and is used for a fluorescence imaging system microscope, the application provides a closed observation cavity which can be fixed on a fluorescence imaging microscope, ultrapure water is added into a groove around the cavity through the accessible, a small-sized electronic hygrometer is placed on one side of an objective table, and the regulation and control of the humidity in the observation cavity filled with the specific sample are realized through external nitrogen purging.
In order to achieve the purpose, the technical scheme of the application is as follows: a microscope objective table closed box capable of being added with a small electronic hygrometer comprises a slide glass bearing table A, a small electronic hygrometer placing groove B, a groove C for containing ultrapure water, a box cover plate D, an air guide pipe E for connecting the box with an external air circuit, and an air outlet pipe F; the sealed box is integrally fixed on the surface of a fluorescence imaging microscope objective table by using an adhesive tape, a light-transmitting hole of a glass slide bearing table A of the box is superposed with a light-transmitting hole of the microscope objective table, an air outlet pipe F is connected with an external nitrogen gas cylinder through an air flow rate regulating valve, and the adjustment and control of the humidity in a box cavity can be realized by combining a reading regulating air flow rate regulating valve of a small electronic hygrometer in the box.
Further, the slide glass bearing table A is used for placing a slide glass with a sample to be observed during testing.
Further, the small electronic hygrometer groove B is provided with a small electronic hygrometer during testing.
Furthermore, the ultrapure water containing groove C contains a proper amount of ultrapure water during testing (the water level is preferably not higher than the nitrogen inlet).
Further, this application still includes the apron of sealed box, seals the fixed mode of box and microscope objective table.
Further, the bottom surface of the closed box is in contact with a microscope stage.
Further, the nitrogen purge used in the experiment was ordinary nitrogen or high-purity nitrogen.
The utility model discloses owing to adopt above technical scheme, can gain following technological effect:
the device has the characteristics of simple structure, low cost, small volume, ingenious design, very simple and convenient operation and the like; the structure not only realizes the in-situ dynamic observation of the sample in the environment with controllable humidity, but also realizes the in-situ dynamic observation in different chemical atmospheres through the replacement of the volatile liquid in the liquid containing groove, skillfully realizes the requirement of realizing special functions with low cost, and provides a simple and easy method for vast front-line scientific research workers.
Drawings
FIG. 1 is a schematic diagram of an apparatus for controlling the humidity of the environment in which a sample is observed on a microscope of a fluorescence imaging system;
the sequence numbers in the figures illustrate: 1. the glass slide bearing platform A is provided with glass slides, the groove B is provided with a small electronic hygrometer, the groove C is provided with ultrapure water, the groove C is provided with an ultrapure water holding groove, the box is connected with an external gas circuit through gas guide tubes E and 5, a connecting line (a cover plate is not shown in the figure) for connecting the box and the cover plate is provided with.
Detailed Description
The invention is described in further detail below with reference to the drawings of embodiments: the present application is further described by taking this as an example.
As shown in fig. 1, the present embodiment provides a device for regulating and controlling the environmental humidity of an observed sample on a fluorescence imaging microscope, which comprises a microscope stage closed box capable of being added with a small electronic hygrometer, and comprises a slide glass bearing platform a, a small electronic hygrometer placing groove B, a groove C for containing ultrapure water, a box cover plate D, an air duct E for connecting the box with an external air path, and an air outlet duct F; the sealed box is integrally fixed on the surface of a fluorescence imaging microscope objective table by using an adhesive tape, a light-transmitting hole of a glass slide bearing table A of the box is superposed with a light-transmitting hole of the microscope objective table, an air outlet pipe F is connected with an external nitrogen gas cylinder through an air flow rate regulating valve, and the adjustment and control of the humidity in a box cavity can be realized by combining a reading regulating air flow rate regulating valve of a small electronic hygrometer in the box.
According to the requirement, the nitrogen flow rate regulating valve can be regulated under the indication of the readings of the small hygrometer to realize the free regulation and control of the humidity in the closed box, so that the fluorescent life imaging and the spectrum acquisition of different samples in the specific humidity environment are realized. The experimental device which is simple, convenient and easy to operate and low in cost is provided for deeply understanding the physical and chemical characteristics of the sample in a high-humidity environment.
The above, only for the utility model discloses create the concrete implementation way of preferred, nevertheless the utility model discloses the protection scope of creation is not limited to this, and any person skilled in this technical field is in the utility model discloses create the technical scope of disclosure, according to the utility model discloses the technical scheme of creation and utility model design equivalence replacement or change all should be covered in the protection scope of creation of the utility model.
Claims (5)
1. A device for regulating and controlling the environmental humidity of a sample on a fluorescence imaging system microscope is characterized in that,
the device comprises a block body with a plane upper surface and a plane lower surface and a cover plate with a plane lower surface, wherein a groove is arranged on the upper surface of the block body, an annular groove is arranged on the upper surface of the block body around the groove, and the groove is positioned in the middle of an area surrounded by the annular groove;
a through hole is formed in the bottom of the groove, a glass slide is arranged at the bottom of the groove and is arranged above the through hole, the peripheral edge above the through hole is attached to the lower surface of the glass slide, and a hygrometer is arranged in the groove;
the cover plate is arranged above the block body, and the upper surface of the block body around the annular groove is attached to the lower surface of the cover plate;
the outer side wall surface of the annular groove is provided with an air inlet through hole and an air outlet through hole which are communicated with the outside of the block body, and the air inlet through hole is connected with a nitrogen source through a pipeline;
the annular groove is internally provided with water, and a gap is reserved between the upper surface of the block body between the annular groove and the upper cover plate and is used as a gas exchange channel.
2. The apparatus of claim 1,
the gas exchange channel is characterized in that the upper surface of the block between the annular groove and the groove is recessed downwards, and the plane of the upper surface of the block between the annular groove and the groove is located below the plane of the upper surfaces of the blocks around the annular groove.
3. The apparatus of claim 1,
and a transparent window is arranged in the middle of the cover plate corresponding to the groove or the cover plate is a transparent cover plate.
4. The apparatus of claim 1,
when the device is used, the device is fixed on the surface of a fluorescence imaging microscope objective table by using an adhesive tape, and a through hole below a glass slide in the device is coaxial with a light-transmitting hole of the microscope objective table.
5. The apparatus of claim 1,
the air outlet through hole is connected with the nitrogen gas cylinder through a gas flow speed regulating valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922212153.3U CN211014833U (en) | 2019-12-11 | 2019-12-11 | Device for regulating and controlling environmental humidity of sample on fluorescence imaging system microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922212153.3U CN211014833U (en) | 2019-12-11 | 2019-12-11 | Device for regulating and controlling environmental humidity of sample on fluorescence imaging system microscope |
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| Publication Number | Publication Date |
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| CN211014833U true CN211014833U (en) | 2020-07-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201922212153.3U Active CN211014833U (en) | 2019-12-11 | 2019-12-11 | Device for regulating and controlling environmental humidity of sample on fluorescence imaging system microscope |
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| CN (1) | CN211014833U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114199869A (en) * | 2020-12-03 | 2022-03-18 | 蝌动科技(成都)有限公司 | Body fluid detection device |
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2019
- 2019-12-11 CN CN201922212153.3U patent/CN211014833U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114199869A (en) * | 2020-12-03 | 2022-03-18 | 蝌动科技(成都)有限公司 | Body fluid detection device |
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