CN210990917U - Pretreatment device before experimental mouse perfusion - Google Patents
Pretreatment device before experimental mouse perfusion Download PDFInfo
- Publication number
- CN210990917U CN210990917U CN201921004187.7U CN201921004187U CN210990917U CN 210990917 U CN210990917 U CN 210990917U CN 201921004187 U CN201921004187 U CN 201921004187U CN 210990917 U CN210990917 U CN 210990917U
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- CN
- China
- Prior art keywords
- pawl
- box body
- pretreatment device
- perfusion
- mouse
- Prior art date
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- Expired - Fee Related
Links
- 230000010412 perfusion Effects 0.000 title claims description 12
- 241000699670 Mus sp. Species 0.000 claims abstract description 17
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical group [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims abstract description 9
- 241000699666 Mus <mouse, genus> Species 0.000 claims description 40
- 238000013016 damping Methods 0.000 claims description 16
- 230000007306 turnover Effects 0.000 claims description 13
- 239000003983 inhalation anesthetic agent Substances 0.000 claims description 3
- 238000002203 pretreatment Methods 0.000 claims 1
- 230000003444 anaesthetic effect Effects 0.000 abstract description 5
- 238000007781 pre-processing Methods 0.000 abstract description 3
- 206010002091 Anaesthesia Diseases 0.000 description 5
- 230000037005 anaesthesia Effects 0.000 description 5
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 108700025906 fos Genes Proteins 0.000 description 3
- 101150078861 fos gene Proteins 0.000 description 3
- 230000014509 gene expression Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- PIWKPBJCKXDKJR-UHFFFAOYSA-N Isoflurane Chemical compound FC(F)OC(Cl)C(F)(F)F PIWKPBJCKXDKJR-UHFFFAOYSA-N 0.000 description 2
- 235000011941 Tilia x europaea Nutrition 0.000 description 2
- 210000000683 abdominal cavity Anatomy 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 239000000292 calcium oxide Substances 0.000 description 2
- 235000012255 calcium oxide Nutrition 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229960002725 isoflurane Drugs 0.000 description 2
- 239000004571 lime Substances 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- RNFNDJAIBTYOQL-UHFFFAOYSA-N chloral hydrate Chemical compound OC(O)C(Cl)(Cl)Cl RNFNDJAIBTYOQL-UHFFFAOYSA-N 0.000 description 1
- 229960002327 chloral hydrate Drugs 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 229960003132 halothane Drugs 0.000 description 1
- BCQZXOMGPXTTIC-UHFFFAOYSA-N halothane Chemical compound FC(F)(F)C(Cl)Br BCQZXOMGPXTTIC-UHFFFAOYSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000004081 narcotic agent Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a preprocessing device before experimental mice fills, it includes the box, set up in the box lower part and be used for placing experimental mice's net pad, set up underframe, the lime hydrate bearing box of setting in the box lateral wall outside, set up bleeder vent between box and lime hydrate bearing box, set up the pipe of dosing at the last inclined opening at box top, set up on the box lateral wall and be used for sending into suction-type anesthetic. The utility model relates to a rationally, compact structure and convenient to use.
Description
Technical Field
The utility model relates to a pretreatment device before experimental mice perfuse.
Background
At present, before the perfusion of the mice, a method (such as urethane or chloral hydrate) of excessively injecting anesthetic into the abdominal cavity after directly holding the mice is generally adopted. The traditional treatment method is easy to cause high expression of fos gene in the brain of a mouse, and the expression condition of the fos gene is an important index reflecting the activation condition of the pre-perfusion treatment on the brain region. There is therefore a need for a treatment that reduces irritation to animals.
Many operators will be bitten by mice because the animal holding method is not standard, and the invention can also greatly reduce the accident rate.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that a preprocessing device before experimental mice perfuse is provided overall.
In order to solve the above problems, the utility model adopts the following technical proposal:
the utility model provides a preprocessing device before experimental mouse fills, includes the box, set up in the box lower part and be used for placing experimental mouse's net pad, set up the underframe of putting up the below at the net, set up the lime hydrate bearing box in the box lateral wall outside, set up the bleeder vent between box and lime hydrate bearing box, set up the opening that inclines upward at the box top, set up on the box lateral wall and be used for sending into the pipe of dosing of suction-type anesthetic.
As a further improvement of the above technical solution:
the mouse inlet is formed in the box body, the root of the turnover plate is hinged to the lower end of the mouse inlet, positioning enclosing plates are vertically arranged on three sides of the upper surface of the turnover plate, and one side of an opening of each positioning enclosing plate corresponds to the mouse inlet.
A pulley yoke is arranged above the mouse inlet, a pair of damping rollers is arranged on the pulley yoke in parallel, one end of a pulling rope is connected to the back of the cantilever end of the turnover plate, and the other end of the pulling rope is exposed out through a gap between the pair of damping rollers.
The end part of the damping roller is coaxially provided with a ratchet wheel, a pawl corresponding to the ratchet wheel is arranged on the pulley frame, one end of the pawl is provided with a limit block, and a reset spring is connected between the pawl and the limit block.
The pulley frame is provided with a reverse elastic push rod, the end part of the reverse elastic push rod is provided with a reverse pushing frame, and the pawl is driven to swing and separate from the ratchet wheel after the reverse pushing frame extends out. The advantageous effects of the present invention are described in more detail in the detailed description of the invention.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Wherein: 1. a box body; 3. a grid pad; 4. a bottom frame; 5. air holes are formed; 6. a lime hydrate bearing box; 7. an upper inclined opening; 8. an administration tube; 10. a mouse inlet; 12. a turnover plate; 13. positioning a coaming; 15. a pulley yoke; 16. a pulling rope; 17. a ratchet wheel; 18. a pawl; 19. a limiting block; 20. a reset spring; 21. a reverse pushing frame; 22. the elastic push rod is reversed.
Detailed Description
As shown in fig. 1, the pretreatment device before the perfusion of the experimental mouse of the embodiment comprises a box body 1, a grid pad 3 arranged at the lower part of the box body 1 and used for placing the experimental mouse, a bottom frame 4 arranged below the grid pad 3, a lime hydrate bearing box 6 arranged at the outer side of the side wall of the box body 1, air holes 5 arranged between the box body 1 and the lime hydrate bearing box 6, an upper inclined opening 7 arranged at the top of the box body 1, and an administration tube 8 arranged on the side wall of the box body 1 and used for feeding inhalation anesthetic.
A mouse inlet 10 is arranged on the box body 1, the lower end of the mouse inlet 10 is hinged with the root of a turnover plate 12, three sides of the upper surface of the turnover plate 12 are vertically provided with positioning surrounding plates 13, and one side of an opening of each positioning surrounding plate 13 corresponds to the mouse inlet 10.
A pulley frame 15 is arranged above the mouse inlet 10, a pair of damping rollers is arranged on the pulley frame 15 in parallel, one end of a pulling rope 16 is connected to the back of the cantilever end of the turnover plate 12, and the other end of the pulling rope 16 is exposed through a gap between the pair of damping rollers.
A ratchet wheel 17 is coaxially arranged at the end part of the damping roller, a pawl 18 corresponding to the ratchet wheel 17 is arranged on the pulley frame 15, a limit block 19 is arranged at one end of the pawl 18, and a return spring 20 is connected between the pawl 18 and the limit block 19.
The pulley frame 15 is provided with a reverse elastic push rod 22, the end part of the reverse elastic push rod 22 is provided with a reverse push frame 21, and the pawl 18 is driven to swing and separate from the ratchet wheel 17 after the reverse push frame 21 extends.
Use the utility model discloses the time, through adopting box 1 (box body) structure, bear the weight of experimental mouse through net pad 3 for quiet health in underframe 4 falls down with the urine clearing hole is avoided polluting in excrement and urine, and the carbon dioxide of mouse exhalation passes through the bleeder vent and gets into lime bearing box 6, and is absorbed by quick lime, improves test effect. Incline down the opening 7 (the opening plate that corresponds does not draw) and conveniently open the mouse of taking, administer 8 convenient narcotics of giving of pencil, in order to solve the problem that the mouse bites, place the mouse simultaneously and avoid startling, place returning face plate 12 with the mouse, location bounding wall 13 prevents that the mouse from escaping, when the mouse oneself does not know to get into mouse mouth 10, make returning face plate 12 slowly become vertical state by the level through tractive rope 16, thereby gentle mouse drives and gets into the box, avoid stinging, avoid startling the mouse simultaneously.
In order to place traction, the overturning plate is enabled to be horizontal through self weight by releasing hands, a damping roller is designed, in order to realize the fixation of any angle of the overturning plate and prevent the overturning plate from being horizontal, a ratchet wheel and pawl structure is designed, and friction force is utilized to be in pressure contact with a rope. When the mouse is sent into once more when needs reset, only need to push reverse elasticity push rod 22 with the hand for it overcomes the spring force, promotes pawl 18 through reverse push frame 21 and overcomes the spring force, and with the ratchet separation, thereby utilizes the gravity of returning face plate, realizes the upset of damping roller, thereby makes the returning face plate become the level, the utility model discloses the noise is little, the flexible operation.
The utility model discloses through the preliminary treatment of suction-type anesthesia earlier before abdominal cavity anesthesia, reduce the stimulation to the animal. Thereby reducing fos gene expression in the central nervous system. For the selection of the type of inhalation anesthetic as a halothane anesthetic (hereinafter isoflurane is used as an example), the safety and anesthetic effect are superior to those of the conventionally selected ether. The bottom box is provided with lime (quicklime) which can be used for absorbing carbon dioxide exhaled by the mouse and increasing the reliability of anesthesia results.
When the mouse is used, the mouse is placed in the device, and the upper cover is closed. When the mouse is stable and is adaptive to the environment, the isoflurane is added into the medicine box through the administration port. And closing the medicine feeding port after the medicine feeding is finished. After the gas anesthesia was completed, the mice were removed. Abdominal anesthesia is performed.
The utility model relates to a rationally, low cost, durable, safe and reliable, easy operation, labour saving and time saving, saving fund, compact structure and convenient to use.
The present invention has been fully described for a clear disclosure, and is not to be construed as an exemplification of the prior art.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; it is obvious to those skilled in the art that a plurality of embodiments of the present invention may be combined. Such modifications and substitutions do not depart from the spirit and scope of the embodiments of the present invention.
Claims (9)
1. The utility model provides a pretreatment device before experimental mice perfuse which characterized in that: the device comprises a box body (1), a grid pad (3) arranged on the lower portion of the box body (1) and used for placing an experimental mouse, a bottom frame (4) arranged below the grid pad (3), a lime hydrate bearing box (6) arranged on the outer side of the side wall of the box body (1), air holes (5) arranged between the box body (1) and the lime hydrate bearing box (6), an upper inclined opening (7) arranged at the top of the box body (1), and an administration tube (8) arranged on the side wall of the box body (1) and used for feeding inhalation anesthetic.
2. The pre-perfusion pretreatment device for laboratory mice according to claim 1, wherein: a mouse inlet (10) is formed in the box body (1), the lower end of the mouse inlet (10) is hinged with the root of a turnover plate (12), positioning enclosing plates (13) are vertically arranged on three sides of the upper surface of the turnover plate (12), and one side of an opening of each positioning enclosing plate (13) corresponds to the mouse inlet (10).
3. The pre-perfusion pretreatment device for laboratory mice according to claim 2, wherein: a pulley frame (15) is arranged above the mouse inlet (10), a pair of damping rollers is arranged on the pulley frame (15) in parallel, one end of a pulling rope (16) is connected to the back of the cantilever end of the turnover plate (12), and the other end of the pulling rope (16) is exposed through a gap between the pair of damping rollers.
4. The pre-perfusion pretreatment device for laboratory mice according to claim 3, wherein: the damping roller is characterized in that a ratchet wheel (17) is coaxially arranged at the end part of the damping roller, a pawl (18) corresponding to the ratchet wheel (17) is arranged on the pulley frame (15), a limiting block (19) is arranged at one end of the pawl (18), and a return spring (20) is connected between the pawl (18) and the limiting block (19).
5. The pre-perfusion pretreatment device for laboratory mice according to claim 4, wherein: a reverse elastic push rod (22) is arranged on the pulley frame (15), a reverse pushing frame (21) is arranged at the end part of the reverse elastic push rod (22), and the pawl (18) is driven to swing and is separated from the ratchet wheel (17) after the reverse pushing frame (21) extends out.
6. The utility model provides a pretreatment device before experimental mice perfuse which characterized in that: a mouse inlet (10) is formed in the box body (1), the lower end of the mouse inlet (10) is hinged with the root of a turnover plate (12), positioning enclosing plates (13) are vertically arranged on three sides of the upper surface of the turnover plate (12), and one side of an opening of each positioning enclosing plate (13) corresponds to the mouse inlet (10).
7. The pre-perfusion pretreatment device for laboratory mice according to claim 6, wherein: a pulley frame (15) is arranged above the mouse inlet (10), a pair of damping rollers is arranged on the pulley frame (15) in parallel, one end of a pulling rope (16) is connected to the back of the cantilever end of the turnover plate (12), and the other end of the pulling rope (16) is exposed through a gap between the pair of damping rollers.
8. The pre-perfusion pretreatment device for laboratory mice according to claim 7, wherein: the damping roller is characterized in that a ratchet wheel (17) is coaxially arranged at the end part of the damping roller, a pawl (18) corresponding to the ratchet wheel (17) is arranged on the pulley frame (15), a limiting block (19) is arranged at one end of the pawl (18), and a return spring (20) is connected between the pawl (18) and the limiting block (19).
9. The pre-treatment device for laboratory mice before perfusion according to claim 8, characterized in that: a reverse elastic push rod (22) is arranged on the pulley frame (15), a reverse pushing frame (21) is arranged at the end part of the reverse elastic push rod (22), and the pawl (18) is driven to swing and is separated from the ratchet wheel (17) after the reverse pushing frame (21) extends out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921004187.7U CN210990917U (en) | 2019-07-01 | 2019-07-01 | Pretreatment device before experimental mouse perfusion |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921004187.7U CN210990917U (en) | 2019-07-01 | 2019-07-01 | Pretreatment device before experimental mouse perfusion |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210990917U true CN210990917U (en) | 2020-07-14 |
Family
ID=71502781
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921004187.7U Expired - Fee Related CN210990917U (en) | 2019-07-01 | 2019-07-01 | Pretreatment device before experimental mouse perfusion |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210990917U (en) |
-
2019
- 2019-07-01 CN CN201921004187.7U patent/CN210990917U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200714 |
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| CF01 | Termination of patent right due to non-payment of annual fee |