CN210974699U - Electric conversion cup - Google Patents
Electric conversion cup Download PDFInfo
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- CN210974699U CN210974699U CN201921787938.7U CN201921787938U CN210974699U CN 210974699 U CN210974699 U CN 210974699U CN 201921787938 U CN201921787938 U CN 201921787938U CN 210974699 U CN210974699 U CN 210974699U
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- electric shock
- electrode
- conducting strip
- groove
- sets
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Abstract
An electrical conversion cup, comprising: conducting strip, electrode, support and electric shock groove, wherein: the conducting strip sets up in support both sides, and the electric shock groove sets up in the support center, and the electrode sets up on the conducting strip and symmetry sets up in electric shock groove both ends. The electrode includes: a cathode and an anode, wherein: one ends of the cathode and the anode are symmetrically arranged on the top of the conducting strip, and the other ends are vertically arranged in the electric shock groove. The electric shock groove center is equipped with the electric shock pond, wherein: the electric shock pool is matched with one end of the electrode. The bracket is provided with a concave structure which is used for being nested with the electric shock groove. The utility model discloses an increase the distance between two electrodes, reduce every electrode face simultaneously and treat the contact width of the cell solution of electric shock to can reduce the death of cell, increase the electric transformation efficiency of cell.
Description
Technical Field
The utility model relates to a technology in the biomedical equipment field, in particular to an electric conversion cup.
Background
Electrotransformation is a transfection means for introducing foreign substances into cells easily and efficiently, and is a highly versatile and simple protocol for introducing nucleic acids, drug components, and the like into cells. The current research suggests that the electric transformation is the transient stimulation of the cell by an external electric field, which gives enough free energy to the cell membrane, so that the rearrangement of phospholipid molecules of the cell membrane occurs, resulting in the generation of hydrophobic holes on the cell membrane. Molecules of exogenous DNA, RNA, and drugs, etc. mixed in the electrotransformation buffer then enter the cell through the pores of the cell membrane (Neumann E, 1982). The application of transient electric fields during electrotransformation allows a significant change in the pH at both electrodes, increasing cell death (Nordenstrom BE, 1994), while the electric heating produced also increases cell death (Pliquett UF, 2002).
SUMMERY OF THE UTILITY MODEL
The utility model discloses the above-mentioned not enough to prior art exists provides an electricity conversion cup, through increasing the distance between two electrodes, reduces every electrode surface simultaneously and treats the contact width of the cell solution of electric shock to can reduce the death of cell, increase the electricity conversion efficiency of cell.
The utility model discloses a realize through following technical scheme:
the utility model discloses a: conducting strip, electrode, support and electric shock groove, wherein: the conducting strip sets up in support both sides, and the electric shock groove sets up in the support center, and the electrode sets up on the conducting strip and symmetry sets up in electric shock groove both ends.
The electrode comprises: a cathode and an anode, wherein: one ends of the cathode and the anode are symmetrically arranged on the top of the conducting strip, and the other ends are vertically arranged in the electric shock groove.
The electric shock groove center be equipped with the electric shock pond, wherein: the electric shock pool is matched with one end of the electrode.
The bracket is provided with a concave structure which is used for being nested with the electric shock groove.
Technical effects
Compared with the prior art, the utility model discloses an increase the distance between two electrodes, reduce every electrode surface simultaneously and treat the contact width of the cell solution of electric shock to can reduce the death of cell, increase the electricity transformation efficiency of cell.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a transverse cross-sectional view of the present invention;
fig. 3 is an exploded view of the present invention;
in the figure: conducting strip 1, electrode 2, support 3, electric shock groove 4, electric shock pond 5, negative pole 6, positive pole 7, concave structure 8.
Detailed Description
As shown in fig. 1, the present embodiment relates to an electric conversion cup, which comprises: conducting strip 1, electrode 2, support 3 and electric shock groove 4, wherein: the conducting plate 1 is arranged at two sides of the bracket 3, the electric shock groove 4 is embedded in the center of the bracket 3, and the electrodes 2 are arranged on the conducting plate 1 and symmetrically arranged at two ends of the electric shock groove 4.
The electrode 2 comprises: a cathode 6 and an anode 7, wherein: one ends of the cathode 6 and the anode 7 are symmetrically arranged on the top of the conducting strip 1, and the other ends are vertically arranged in the electric shock groove 4.
The material of electric shock groove 4 be PDMS (polydimethylsiloxane), the center is equipped with electric shock pond 5, wherein: the electrodes 2 are arranged in the cuvette 5.
The bracket 3 is provided with a concave structure 8 which is used for being nested with the electric shock groove 4.
The thickness range of the conducting strip 1 is 0.5mm-1mm, the length range is 40mm-50mm, and the width range is 12mm-15 mm.
The thickness range of the electrode 2 is 0.5mm-1mm, the length range is 10mm-15mm, and the width range is 0.5mm-1 mm.
The height range of the bracket 3 is 40mm-50mm, the length range is 12mm-15mm, the thickness range is 12mm-15mm, the height range of the concave structure 8 is 10mm-15mm, the length range is 6mm-10mm, and the thickness range is 12mm-15 mm.
The electric shock groove 4 has a height range of 10mm-15mm, a length range of 6mm-10mm and a thickness range of 12mm-15 mm.
The length range of the electric shock pool 5 is 10mm-15mm, the width range is 0.5mm-1mm, and the depth range is 5mm-10 mm.
The experimental process comprises the following steps: culturing mouse primary SVF cell to 80% action density, counting after trypsinizing cell, taking 30x104The cells are resuspended by 30 mu L electrotransformation buffer solution after centrifugation, then 3ug of plasmid pmaxGFP (mammalian cell enhanced green fluorescent expression vector) is added and mixed evenly, the mixed solution is transferred into the electrotransformation cup of the utility model, the electric shock is carried out according to the program 300V, 20ms, twice and 100ms interval, after the electric shock, the cells in the electrotransformation cup are transferred into a 12-hole plate, 2m L DMEM (containing 10% serum) culture medium is added for continuous culture, after 24 hours, the transfection effect is observed under a fluorescent microscope, and the primary SVF cells of the mouse can express GFP (green fluorescent protein) with high efficiency after transfection.
The foregoing embodiments may be modified in various ways by those skilled in the art without departing from the spirit and scope of the present invention, which is not limited by the above embodiments but is to be accorded the full scope defined by the appended claims, and all such modifications and variations are within the scope of the invention.
Claims (4)
1. An electrical conversion cup, comprising: conducting strip, electrode, support and electric shock groove, wherein: the conducting strip sets up in support both sides, and the electric shock groove sets up in the support center, and the electrode sets up on the conducting strip and symmetry sets up in electric shock groove both ends.
2. The electrical conversion cup as set forth in claim 1, comprising: the electrode comprises: a cathode and an anode, wherein: one ends of the cathode and the anode are symmetrically arranged on the top of the conducting strip, and the other ends are vertically arranged in the electric shock groove.
3. The electrical conversion cup as claimed in claim 1, wherein a shock tank is provided at the center of the shock tank, wherein: the electric shock pool is matched with one end of the electrode.
4. The electrical conversion cup as defined in claim 1, wherein said frame has a concave structure for nesting with the shock slot.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921787938.7U CN210974699U (en) | 2019-10-23 | 2019-10-23 | Electric conversion cup |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921787938.7U CN210974699U (en) | 2019-10-23 | 2019-10-23 | Electric conversion cup |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210974699U true CN210974699U (en) | 2020-07-10 |
Family
ID=71456648
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921787938.7U Expired - Fee Related CN210974699U (en) | 2019-10-23 | 2019-10-23 | Electric conversion cup |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210974699U (en) |
-
2019
- 2019-10-23 CN CN201921787938.7U patent/CN210974699U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200710 Termination date: 20211023 |