CN210960122U - Embryonic stem cell transplanting device - Google Patents
Embryonic stem cell transplanting device Download PDFInfo
- Publication number
- CN210960122U CN210960122U CN201921298655.6U CN201921298655U CN210960122U CN 210960122 U CN210960122 U CN 210960122U CN 201921298655 U CN201921298655 U CN 201921298655U CN 210960122 U CN210960122 U CN 210960122U
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- Prior art keywords
- fixed
- liquid nitrogen
- fixed pipe
- air inlet
- inlet port
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- 210000001671 embryonic stem cell Anatomy 0.000 title claims abstract description 23
- 238000011476 stem cell transplantation Methods 0.000 claims abstract description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 100
- 239000007788 liquid Substances 0.000 abstract description 50
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 50
- 238000000034 method Methods 0.000 abstract description 13
- 210000000130 stem cell Anatomy 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 4
- 210000001161 mammalian embryo Anatomy 0.000 abstract description 3
- 239000007789 gas Substances 0.000 abstract 1
- 238000005457 optimization Methods 0.000 description 4
- 238000002054 transplantation Methods 0.000 description 4
- 230000000903 blocking effect Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000007045 gastrulation Effects 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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Abstract
The utility model discloses an embryonic stem cell transplantation device, including holding vessel, cover, gas filled structure, fixed pipe, fixing base, spring, inlet port and stopper, fixed stopper and buffer structure. The utility model has the advantages that: through communicating liquid nitrogen conveying pipe and inlet port, press down the liquid nitrogen conveying pipe downwards, make the fixing base lapse, promote the stopper to slide to the bottom direction of holding vessel, make the spring elongate and produce elasticity, stopper lapse in-process drives the inlet port lapse, it is sealed between stopper and the fixed pipe, make the bottom lapse that is the inlet port of Y shape structure break away from the inside wall of fixed pipe, make the inside bottom of inlet port and the inside intercommunication of holding vessel, open the valve of liquid nitrogen storage tank, make liquid nitrogen conveying pipe input liquid nitrogen, make the liquid nitrogen enter into the inside back of inlet port, send into the inside of holding vessel with the liquid nitrogen slope through the inlet port, prevent that the in-process that the liquid nitrogen fills from making the inside embryo stem cell of holding vessel damage.
Description
Technical Field
The utility model relates to a transplantation device specifically is an embryonic stem cell transplantation device, belongs to stem cell technical field.
Background
Embryonic stem cells are a class of cells isolated from early embryos (pre-gastrulation) or primitive gonads that have the property of immortalization, self-renewal, and multidirectional differentiation in vitro cultures, and can be induced to differentiate into almost all cell types in the body, both in vitro and in vivo.
Embryonic stem cell need put into the holding vessel that fills into liquid nitrogen and preserve at the transplantation in-process, prevents that embryonic stem cell from dying at the transplantation in-process, and the holding vessel that traditional embryonic stem cell used is when adding liquid nitrogen, and the lid mouth department that the holding vessel was followed easily to the liquid nitrogen reveals, causes the loss of liquid nitrogen, and the increase cost is unfavorable for embryonic stem cell's storage transplantation.
SUMMERY OF THE UTILITY MODEL
The present invention aims to provide an embryonic stem cell transplantation device for solving the above problems, which is beneficial to the storage of stem cells and avoids the loss of liquid nitrogen.
The utility model realizes the above purpose by the following technical proposal, the embryonic stem cell transplantation device comprises a storage tank, the top end of the storage tank is provided with a tank cover, the tank cover is in threaded connection with the storage tank, the tank cover is connected with an inflation structure, the inflation structure comprises a fixed pipe, a fixed seat, a spring, an air inlet hole and a limiting block, the inner side wall of the tank cover is fixed with the fixed pipe, the fixed pipe extends to the inside of the storage tank, the inner side wall of the fixed pipe is in sliding connection with the limiting block which is in a T-shaped structure, the bottom end of the limiting block is positioned in the storage tank, the spring is fixed between the limiting block and the side wall of the fixed pipe, the fixed pipe penetrates through the spring, the inside of the limiting block is provided with the air inlet hole, the top end of the limiting block is communicated with the inside of the fixed, the top of stopper is fixed with the fixing base, sliding connection between fixing base and the fixed pipe, the fixing base with the inlet port intercommunication.
Preferably, in order to prevent the leakage caused by adding liquid nitrogen into the storage tank, the air inlet hole is of a Y-shaped structure, the top end of the air inlet hole is communicated with the inside of the fixed pipe, and the two through holes at the bottom end of the air inlet hole are abutted to the inner side wall of the fixed pipe.
Preferably, for the convenience of liquid nitrogen pipeline inserts inside the fixing base and aerifys, the fixing base is the spill structure, the bottom of fixing base with the top of inlet port is contradicted, the inside wall threaded connection of fixed pipe has the fixed stopper, the fixed stopper is located the top of fixing base.
Preferably, in order to facilitate the communication between the air inlet hole and the storage tank, the minimum distance between the bottom end of the fixed pipe and the bottom end of the air inlet hole is smaller than the length of the spring, and the diameter of the bottom end of the limiting block is larger than that of the fixed pipe.
Preferably, for the convenience of reducing the impact of liquid nitrogen to stem cells, the internally connected with buffer structure of holding vessel, buffer structure includes fixed block and blocking ball, the inside wall symmetry of holding vessel is fixed with two and is the arc structure the fixed block, two the lateral wall of fixed block all is fixed with a plurality ofly block the ball, deviating from of fixed block one side of the inside wall of holding vessel with the bottom lateral wall of inlet port is tangent.
The utility model has the advantages that: the utility model discloses a communicating liquid nitrogen delivery pipe with the inlet port, press the liquid nitrogen delivery pipe downwards, make the fixing base lapse, promote the stopper to slide to the bottom direction of holding vessel, make the spring elongate and produce elasticity, stopper lapse in-process drives the inlet port lapse, seal between stopper and the fixed tube, make the bottom lapse of the inlet port that is Y-shaped structure break away from the inside wall of fixed tube, make the inside bottom of inlet port and the inside of holding vessel communicate, open the valve of liquid nitrogen storage tank, make liquid nitrogen delivery pipe input liquid nitrogen, make liquid nitrogen enter into the inlet port after inside, send liquid nitrogen into the inside of holding vessel through the inlet port slope, prevent that the liquid nitrogen from filling in-process makes the embryo stem cell inside the holding vessel damage, protect the embryo stem cell in the culture dish inside the holding vessel, after the inside of holding vessel is filled with liquid nitrogen, through slowly upwards lifting liquid nitrogen conveying pipe, make one kind of bottom of liquid nitrogen conveying pipe contradict with the lateral wall of fixing base, the elasticity that the stopper passes through the spring restores the normal position, after the bottom that drives the inlet port slides in the inside wall of fixed pipe again, blocks up fixed pipe, and the inside liquid nitrogen of holding vessel can not flow the outside of holding vessel at the in-process that fills at this moment to prevent that the inside liquid nitrogen of holding vessel from revealing at the in-process that fills, thereby do benefit to embryonic stem cell's storage.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the connection of the inflatable structure and the tank lid shown in FIG. 1;
fig. 3 is an enlarged schematic view of a portion a shown in fig. 2.
In the figure: 1. storage tank, 2, cover, 3, inflatable structure, 31, fixed pipe, 32, fixing base, 33, spring, 34, inlet port, 35, stopper, 4, fixed stopper, 5, buffer structure, 51, fixed block, 52, blocking ball.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-3, an embryonic stem cell transplantation device includes a storage tank 1, a tank cover 2 is disposed at a top end of the storage tank 1, the tank cover 2 is in threaded connection with the storage tank 1, the tank cover 2 is connected with an inflation structure 3, the inflation structure 3 includes a fixed tube 31, a fixed seat 32, a spring 33, an air inlet 34 and a limit block 35, the fixed tube 31 is fixed to an inner side wall of the tank cover 2, the fixed tube 31 extends into the storage tank 1, the limit block 35 having a T-shaped structure is slidably connected to an inner side wall of the fixed tube 31, a bottom end of the limit block 35 is located inside the storage tank 1, the spring 33 is fixed between the limit block 35 and the side wall of the fixed tube 31, the fixed tube 31 penetrates through the spring 33, the air inlet 34 is disposed inside the limit block 35, the top of the air inlet 34 is communicated with the inside of the fixed tube 31, the top of the limiting block 35 is fixed with the fixed seat 32, the fixed seat 32 is connected with the fixed tube 31 in a sliding manner, and the fixed seat 32 is communicated with the air inlet 34.
As a technical optimization scheme of the utility model, inlet port 34 is the Y-shaped structure, inlet port 34 the top with the inside intercommunication of fixed pipe 31, two through-holes of inlet port 34's bottom all with fixed pipe 31's inside wall is contradicted.
As a technical optimization scheme of the utility model, fixing base 32 is the spill structure, the bottom of fixing base 32 with the top of inlet port 34 is contradicted, the inside wall threaded connection of fixed pipe 31 has solid fixed plug 4, gu fixed plug 4 is located the top of fixing base 32.
As a technical optimization scheme of the utility model, the bottom of fixed pipe 31 with minimum distance between the bottom of inlet port 34 is less than the length of spring 33, the diameter of stopper 35 bottom is greater than the diameter of fixed pipe 31.
As a technical optimization scheme of the utility model, the internal connection of holding vessel 1 has buffer structure 5, buffer structure 5 includes fixed block 51 and blocks ball 52, the inside wall symmetry of holding vessel 1 is fixed with two and is the arc structure fixed block 51, two the lateral wall of fixed block 51 all is fixed with a plurality ofly block ball 52, fixed block 51 deviate from one side of 1 inside wall of holding vessel with the bottom lateral wall of inlet port 34 is tangent.
When the utility model is used, firstly, the cover 2 is rotated to separate the cover 2 from the storage tank 1, the culture dish with embryonic stem cells is placed in the storage tank 1, the cover 2 is closed, the liquid nitrogen delivery pipe of the liquid nitrogen storage tank is inserted into the fixing base 32 to communicate the liquid nitrogen delivery pipe with the air inlet 34, the liquid nitrogen delivery pipe is pressed downwards to slide the fixing base 32 downwards, the limiting block 35 is pushed to slide towards the bottom end of the storage tank 1 to make the spring 33 elongate to generate elasticity, the limiting block 35 drives the air inlet 34 to slide downwards in the downward sliding process, the space between the limiting block 35 and the fixing pipe 31 is sealed, the bottom end of the air inlet 34 in a Y-shaped structure slides downwards to separate from the inner side wall of the fixing pipe 31, the inner bottom end of the air inlet 34 is communicated with the interior of the storage tank 1, at this time, the fixing plug 4 is rotated to make the fixing plug 4 extrude the liquid nitrogen delivery pipe, opening a valve of a liquid nitrogen storage tank, inputting liquid nitrogen into a liquid nitrogen delivery pipe, enabling the liquid nitrogen to enter the air inlet 34, then sending the liquid nitrogen into the storage tank 1 obliquely through the air inlet 34, preventing the embryonic stem cells in the storage tank 1 from being damaged in the process of filling the liquid nitrogen, protecting the embryonic stem cells in a culture dish in the storage tank 1, after the storage tank 1 is filled with the liquid nitrogen, rotating a fixing plug 4 to enable the fixing plug 4 to be far away from the liquid nitrogen delivery pipe, enabling the bottom end of the liquid nitrogen delivery pipe to be abutted against the side wall of a fixing seat 32 by slowly lifting the liquid nitrogen delivery pipe upwards until a limiting block 35 is restored to the original position through the elastic force of a spring 33, driving the bottom end of the air inlet 34 to slide into the inner side wall of a fixing pipe 31 again, blocking the fixing pipe 31, and then preventing the liquid nitrogen in the storage tank 1 from flowing out of the storage tank 1 in, thereby prevent that the inside liquid nitrogen of holding vessel 1 from revealing at the in-process that fills to do benefit to embryonic stem cell's storage, in the in-process of inlet port 34 ventilating, liquid nitrogen fills the in-process and blows to a plurality of jam balls 52 on fixed block 51, reduces the speed that liquid nitrogen fills, makes liquid nitrogen slowly get into holding vessel 1, avoids embryonic stem cell to receive the impact of liquid nitrogen and the damage appears.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (5)
1. An embryonic stem cell transplantation device comprising a storage tank (1), characterized in that: the storage tank is characterized in that a tank cover (2) is arranged at the top end of the storage tank (1), the tank cover (2) is in threaded connection with the storage tank (1), the tank cover (2) is connected with an inflation structure (3), the inflation structure (3) comprises a fixed pipe (31), a fixed seat (32), a spring (33), an air inlet hole (34) and a limiting block (35), the inner side wall of the tank cover (2) is fixed with the fixed pipe (31), the fixed pipe (31) extends to the inside of the storage tank (1), the inner side wall of the fixed pipe (31) is in sliding connection with the limiting block (35) in a T-shaped structure, the bottom end of the limiting block (35) is located inside the storage tank (1), the spring (33) is fixed between the limiting block (35) and the side wall of the fixed pipe (31), and the fixed pipe (31) penetrates through the inside of the spring (33), an air inlet hole (34) is formed in the limiting block (35), the top end of the air inlet hole (34) is communicated with the inside of the fixed pipe (31), the top end of the limiting block (35) is fixed with the fixed seat (32), the fixed seat (32) is connected with the fixed pipe (31) in a sliding mode, and the fixed seat (32) is communicated with the air inlet hole (34).
2. An embryonic stem cell transplantation device according to claim 1, wherein: the air inlet holes (34) are Y-shaped structures, the top ends of the air inlet holes (34) are communicated with the inside of the fixed pipe (31), and two through holes in the bottom ends of the air inlet holes (34) are abutted to the inner side wall of the fixed pipe (31).
3. An embryonic stem cell transplantation device according to claim 1, wherein: the fixing seat (32) is of a concave structure, the bottom end of the fixing seat (32) is abutted to the top end of the air inlet hole (34), the inner side wall of the fixing pipe (31) is in threaded connection with a fixing plug (4), and the fixing plug (4) is located at the top end of the fixing seat (32).
4. An embryonic stem cell transplantation device according to claim 1, wherein: the minimum distance between the bottom end of the fixed pipe (31) and the bottom end of the air inlet hole (34) is smaller than the length of the spring (33), and the diameter of the bottom end of the limiting block (35) is larger than that of the fixed pipe (31).
5. An embryonic stem cell transplantation device according to claim 1, wherein: the internally connected with buffer structure (5) of holding vessel (1), buffer structure (5) are including fixed block (51) and block ball (52), the inside wall symmetry of holding vessel (1) is fixed with two and is the arc structure fixed block (51), two the lateral wall of fixed block (51) all is fixed with a plurality ofly block ball (52), deviating from of fixed block (51) one side of holding vessel (1) inside wall with the bottom lateral wall of inlet port (34) is tangent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921298655.6U CN210960122U (en) | 2019-08-12 | 2019-08-12 | Embryonic stem cell transplanting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921298655.6U CN210960122U (en) | 2019-08-12 | 2019-08-12 | Embryonic stem cell transplanting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210960122U true CN210960122U (en) | 2020-07-10 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921298655.6U Expired - Fee Related CN210960122U (en) | 2019-08-12 | 2019-08-12 | Embryonic stem cell transplanting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210960122U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113978130A (en) * | 2021-10-22 | 2022-01-28 | 肥城新华印刷有限公司 | Structure and method for realizing rapid color conversion of spray head |
-
2019
- 2019-08-12 CN CN201921298655.6U patent/CN210960122U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113978130A (en) * | 2021-10-22 | 2022-01-28 | 肥城新华印刷有限公司 | Structure and method for realizing rapid color conversion of spray head |
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| GR01 | Patent grant | ||
| PP01 | Preservation of patent right | ||
| PP01 | Preservation of patent right |
Effective date of registration: 20201130 Granted publication date: 20200710 |
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| PD01 | Discharge of preservation of patent | ||
| PD01 | Discharge of preservation of patent |
Date of cancellation: 20231130 Granted publication date: 20200710 |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200710 |