CN210953803U - Ultraviolet visible fluorescence spectrometer - Google Patents
Ultraviolet visible fluorescence spectrometer Download PDFInfo
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- CN210953803U CN210953803U CN201920931645.5U CN201920931645U CN210953803U CN 210953803 U CN210953803 U CN 210953803U CN 201920931645 U CN201920931645 U CN 201920931645U CN 210953803 U CN210953803 U CN 210953803U
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Abstract
The embodiment of the utility model discloses ultraviolet visible fluorescence spectrum appearance. It comprises a light source for emitting incident light covering the full band; the monochromator is used for converting the incident light into monochromatic light with target frequency and emitting the monochromatic light; the focusing device is used for focusing the monochromatic light; the fluorescence detection cell and the transmission detection cell are arranged on the focused monochromatic light path and are respectively used for containing a sample to be subjected to fluorescence detection and a sample to be subjected to transmission detection; a fluorescence detector for detecting the excited fluorescence signal; the transmission detector is used for detecting an ultraviolet light signal; the signal processor is respectively connected with the fluorescence detector and the transmission detector and is used for processing the fluorescence signal and the ultraviolet light signal; the controller is respectively connected with the light source and the monochromator and is used for controlling the light source to emit light and controlling the target frequency of the monochromatic light emitted by the monochromator.
Description
Technical Field
The utility model relates to a check out test set technical field especially relates to a visible fluorescence spectra appearance of ultraviolet.
Background
The existing optical analysis or spectroscopic analysis methods are widely used in quantitative or semi-quantitative determination of substances in various fields. For example, the analysis and determination of pollutants such as petroleum, animal and vegetable oil, and catering oil fume in various water bodies.
In realizing the utility model discloses the in-process, utility model people discovers that relevant technique has following problem: existing detection instruments focus primarily on single visible or single ultraviolet detection. Part of the ultraviolet visible fluorescence spectrometer can realize the single fluorescence detection function. There are also some uv-vis fluorescence spectrometers that can combine more wavelengths. However, the functions of these ultraviolet-visible fluorescence spectrometers are still single, and cannot well meet the increasing use demands of users, and it is difficult to consider different use scenarios.
SUMMERY OF THE UTILITY MODEL
To the technical problem, the embodiment of the utility model provides a possess the ultraviolet visible fluorescence spectrum appearance of ultraviolet visible light detection and fluorescence detection to solve current detecting instrument function singleness, be difficult to satisfy user demand's problem.
A first aspect of the embodiments of the present invention provides an ultraviolet visible fluorescence spectrometer. The ultraviolet visible fluorescence spectrometer comprises: a light source; the light source is used for emitting incident light covering the full wave band; a monochromator; the monochromator is used for converting the incident light into monochromatic light with target frequency and emitting the monochromatic light; a focusing device for focusing the monochromatic light; the detection cell comprises a fluorescence detection cell and a transmission detection cell which are arranged on the focused monochromatic light path and are respectively used for containing a sample to be subjected to fluorescence detection and a sample to be subjected to transmission detection; a fluorescence detector disposed opposite the fluorescence detection cell for detecting an excited fluorescence signal; the transmission detector is arranged at the position opposite to the transmission detection cell and used for detecting an ultraviolet light signal; the signal processor is respectively connected with the fluorescence detector and the transmission detector and is used for processing the fluorescence signal and the ultraviolet light signal; and the controller is respectively connected with the light source and the monochromator and is used for controlling the light source to emit light and controlling the target frequency of the monochromatic light emitted by the monochromator.
Optionally, the uv-vis fluorescence spectrometer further comprises: a modulator and a first focusing lens; the modulator is connected with the controller, and light emitted by the light source sequentially passes through the modulator and the first focusing lens to form incident light which enters the monochromator.
Optionally, the monochromator comprises an incident slit, an exit slit, a grating, a first collimating mirror and a second collimating mirror; the incident light enters from the incident crack, sequentially passes through the first collimating mirror, the grating and the second collimating mirror, and is converted into monochromatic light to be emitted from the emergent crack.
Optionally, the monochromator further comprises a motor; the grating is arranged on the rotating mechanism and is driven by the motor to change the angle; the controller is connected with the motor and controls the rotation angle of the grating through the motor.
Optionally, the grating is a diffraction grating with a density of 1200 lines/mm; the monochromator is a C-T achromatic monochromator.
Optionally, the motor drives rotation of the grating via a ball screw.
Optionally, the uv-vis fluorescence spectrometer further comprises a reference circuit detector, and the reference circuit detector is disposed at a position opposite to the exit slit and is configured to detect and determine the offset and attenuation of the light source.
Optionally, the fluorescence detector is a photomultiplier tube; the transmission detector is a silicon photodiode or a photomultiplier tube.
Optionally, the light source is a xenon lamp, covering wavelengths of 200nm to 800 nm.
Optionally, a first lens group and a second lens group are respectively arranged between the fluorescence detection cell and the fluorescence detector and between the transmission detection cell and the transmission detector; signal energy reaching the fluorescence detector and the transmission detector is boosted by the first lens group and the second lens group.
The embodiment of the utility model provides a multi-functional ultraviolet visible fluorescence spectrum appearance can be with the light path from the visible light to the ultraviolet ray full coverage. Therefore, the device can be used for transmission detection and fluorescence scattering detection. The optical paths of the two optical circuits are compatible in design structure, and the coverage range is wide. In addition, aiming at different testing methods, the multifunctional ultraviolet visible fluorescence spectrometer can complete corresponding detection under a specific testing wavelength.
Drawings
Fig. 1 is a schematic diagram of an embodiment of an ultraviolet-visible fluorescence spectrometer according to an embodiment of the present invention;
fig. 2 is a schematic diagram of an embodiment of a software architecture of an upper computer according to an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those skilled in the art without creative efforts belong to the protection scope of the present invention.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may be present. As used herein, the terms "vertical," "horizontal," "left," "right," "upper," "lower," "inner," "outer," "bottom," and the like are used in an orientation or positional relationship indicated based on the orientation or positional relationship shown in the drawings for convenience in describing the present invention and to simplify the description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items. Furthermore, the technical features mentioned in the different embodiments of the invention described below can be combined with each other as long as they do not conflict with each other.
Fig. 1 is a schematic structural diagram of an ultraviolet-visible fluorescence spectrometer provided by an embodiment of the present invention. As shown in fig. 1, the uv-vis fluorescence spectrometer includes: a light source 11, a monochromator 12, a focusing device 13, a fluorescence detection cell 14, a transmission detection cell 15, a fluorescence detector 16, a transmission detector 17, a signal processor 18, and a controller 19.
Wherein the light source 11 is used for emitting incident light covering the full wave band. Specifically, the light source is a 150W xenon lamp, the wavelength of the xenon lamp is 200nm to 800nm, the light source is modulated into a pulse light source through chopping, and a background signal generated by dark current is eliminated by the photomultiplier.
Preferably, a reflector plated with a reinforced aluminum film can be used to ensure sufficient reflectivity of the ultraviolet section and eliminate chromatic aberration as much as possible.
The monochromator 12 is a light separation device for converting the incident light into monochromatic light of a target frequency and emitting the monochromatic light. The focusing means 13 is for focusing the monochromatic light.
The fluorescence detection cell 14 and the transmission detection cell 15 are both arranged on the focused light path of the monochromatic light and are respectively used for containing a sample to be subjected to fluorescence detection and a sample to be subjected to transmission detection to finish ultraviolet visible light and fluorescence detection.
Specifically, the fluorescence detection cell is a 1cm, four-way colorimetric cell. And the transmission detection cell 15 is a 2cm cuvette that allows parallel light to pass through.
As shown in FIG. 1, the fluorescence detector 16 is disposed opposite the fluorescence detection cell 14 for detecting the excited fluorescence signal. The transmission detector 17 is disposed at a position opposite to the transmission detection cell 15, and is configured to detect an ultraviolet light signal.
Specifically, the fluorescence detector is a photomultiplier tube with high sensitivity. The transmission detector can select a silicon photodiode or a photomultiplier according to the requirement or the cost requirement.
In some embodiments, a first lens group 20 and a second lens group 21 are disposed between the fluorescence detection cell and the fluorescence detector and between the transmission detection cell and the transmission detector, respectively.
By the first lens group 20 and the second lens group 21, signal energy reaching the fluorescence detector and the transmission detector can be boosted as much as possible.
The signal processor 18 is connected to the fluorescence detector and the transmission detector, respectively, for processing the fluorescence signal and the ultraviolet light signal.
Specifically, the signal processor 18 performs a current-to-voltage conversion circuit through the transconductance amplifier, performs a complete digital-to-analog conversion task by using a high-speed AD, and synchronizes the design idea of the acquisition scheme, wherein the chopping pulses drive the acquisition command, trigger the acquisition and buffer the acquisition command into the MCU, and send the acquisition command to the upper computer in real time to acquire data.
The controller 19 is a control core. The monochromator is connected with the light source and the monochromator respectively and used for controlling the light source to emit light and controlling the target frequency of the monochromatic light emitted by the monochromator.
In some embodiments, the uv-vis fluorescence spectrometer further comprises: a modulator 22 and a first focusing lens 13. The modulator 22 is connected to the controller 19 to complete the modulation of the light source. The first focusing lens 13 focuses the light emitted by the light source.
After light emitted by the light source 11 passes through the modulator 22 and the first focusing lens 13 in sequence, incident light is formed and enters the monochromator.
In other embodiments, the uv-vis fluorescence spectrometer further includes a reference circuit detector 23, which is disposed at a position opposite to the exit slit, and can be used to detect and determine the offset and attenuation of the light source, and prompt a user to perform zero point correction. Of course, the automatic zero calibration function can also be performed through a software algorithm of the upper computer.
In daily use, the ultraviolet visible fluorescence spectrometer is matched with an upper computer to complete a detection task. For example, the signal received by the detector is processed by the signal processor and then sent to the upper computer, and the upper computer displays the final detection result after operation.
Specifically, the upper computer can be any type of computer capable of meeting the use requirement. Fig. 2 is a schematic diagram of a software architecture of the upper computer provided by the embodiment of the present invention.
As shown in fig. 2, the upper computer includes a blank management module 210, a standard curve management module 220, a detection data management module 230, a software setting module 240, and a sample detection module 250.
The blank management module 210 may be configured to manage a plurality of stored blank data. The standard curve management module 220 may be configured to manage and store a plurality of standard curves, and perform operations of adding, changing, and deleting.
The test data management module 230 may be used for functions of querying, modifying, deleting, exporting, printing, and previewing existing test data. The software setting module 240 is a functional module for setting, saving, reading, modifying and the like of the instrument parameters and the sample detection parameters.
The sample detection module 250 is a core module for operation, and provides a plurality of detection modes, such as blank detection, standard curve making, sample detection, detection result displaying, and the like.
Referring to fig. 1, the monochromator 12 may specifically include: an entrance slit 121, an exit slit 122, a grating 123, a first collimating mirror 124, and a second collimating mirror 125.
Specifically, the grating 123 is a diffraction grating with a density of 1200 lines/mm, and the monochromator is a C-T achromatic monochromator.
In some embodiments, the monochromator 12 further comprises a motor 126 and a rotation mechanism 127. The grating 123 is mounted on a rotation mechanism 127 and is driven by the motor 126 to change the angle. Specifically, the motor drives the grating to rotate through a ball screw, so that scanning can be completed quickly, and the repeatability is high.
In the using process, the incident light enters from the incident slit 121, and after sequentially passing through the first collimating mirror 124, the grating 123 and the second collimating mirror 125, the incident light is converted into monochromatic light and emitted from the exit slit 122. The controller comprises a signal transmitter, a signal receiver, a signal converter and a plurality of control valves, wherein the signal transmitter is connected with the control valves through the signal converter, and the signal receiver is also connected with the control valves through the signal converter. The controller signal is a type commonly used by those skilled in the art, and the controller signal is not described herein again as long as it can realize basic functions such as signal receiving, transmitting, converting, and the like.
The controller 19 is connected to the motor 126. When the wavelength is located, the upper computer sends a wavelength locating command, the controller 19 controls the stepping motor 126 to operate through command transmission starting and the wavelength command, and moves to a fixed-point wavelength position (the stepping motor 126 can realize accurate control through the subdivision function of the driver) to control the rotation angle of the grating through the motor 126, so that the frequency of the emitted monochromatic light is changed, and the target wavelength is located.
In a preferred embodiment, the optical path of the whole optical system can be in the form of a dual beam to reduce the influence of unstable light source as much as possible. In addition, the optical system is arranged on the integrally designed base platform, so that the interference of external vibration is effectively reduced.
The ultraviolet visible fluorescence spectrometer can meet the detection of the lowest concentration value of oil in water (concentration value in national water environment quality standard) in the standard of domestic sanitary drinking water, and supports two detection modes of measuring an absorbance value at 225nm by adopting an ultraviolet spectrophotometry and measuring a fluorescence intensity value under the conditions that the excitation wavelength is 310nm and the emission wavelength is 360nm by adopting a fluorescence spectrophotometry.
It should be understood that equivalent alterations and modifications can be made by those skilled in the art according to the technical solution of the present invention and the inventive concept, and all such alterations and modifications shall fall within the scope of the appended claims.
Claims (10)
1. An ultraviolet-visible fluorescence spectrometer, comprising: the device comprises a light source, a monochromator, a focusing device, a detection cell, a fluorescence detector, a transmission detector, a signal processor and a controller;
the light source is used for emitting incident light covering the full wave band; the monochromator is used for converting the incident light into monochromatic light with target frequency and emitting the monochromatic light; the focusing device is used for focusing the monochromatic light;
the detection cell comprises a fluorescence detection cell and a transmission detection cell which are arranged on the focused monochromatic light path and are respectively used for containing a sample to be subjected to fluorescence detection and a sample to be subjected to transmission detection;
the fluorescence detector is arranged at a position opposite to the fluorescence detection pool and is used for detecting an excited fluorescence signal; the transmission detector is arranged at the opposite position of the transmission detection pool and is used for detecting an ultraviolet light signal;
the signal processor is respectively connected with the fluorescence detector and the transmission detector and is used for processing the fluorescence signal and the ultraviolet light signal;
the controller is respectively connected with the light source and the monochromator and is used for controlling the light source to emit light and controlling the target frequency of the monochromatic light emitted by the monochromator;
the controller comprises a signal transmitter, a signal receiver, a signal converter and a plurality of control valves, wherein the signal transmitter is connected with the control valves through the signal converter, and the signal receiver is also connected with the control valves through the signal converter.
2. The uv-vis fluorescence spectrometer of claim 1, further comprising: a modulator and a first focusing lens;
the modulator is connected with the controller, and light emitted by the light source sequentially passes through the modulator and the first focusing lens to form incident light which enters the monochromator.
3. The uv-vis fluorescence spectrometer of claim 1, wherein the monochromator comprises an entrance slit, an exit slit, a grating, a first collimating mirror, and a second collimating mirror;
the incident light enters from the incident crack, sequentially passes through the first collimating mirror, the grating and the second collimating mirror, and is converted into monochromatic light to be emitted from the emergent crack.
4. The uv-vis fluorescence spectrometer of claim 3, wherein the monochromator further comprises a motor; the grating is arranged on the rotating mechanism and is driven by the motor to change the angle; the controller is connected with the motor and controls the rotation angle of the grating through the motor.
5. The uv-vis fluorescence spectrometer of claim 3, wherein the grating is a diffraction grating with a density of 1200 lines/mm; the monochromator is a C-T achromatic monochromator.
6. The UV-Vis fluorescence spectrometer of claim 4, wherein the motor drives the grating to rotate through a ball screw.
7. The uv-vis fluorescence spectrometer of claim 3, further comprising a reference circuit detector disposed opposite the exit slit for detecting the deviation and attenuation of the diagnostic light source.
8. The uv-vis fluorescence spectrometer of claim 1, wherein the fluorescence detector is a photomultiplier tube; the transmission detector is a silicon photodiode or a photomultiplier tube.
9. The uv-vis fluorescence spectrometer of claim 1, wherein the light source is a xenon lamp covering wavelengths from 200nm to 800 nm.
10. The uv-vis fluorescence spectrometer according to claim 1, wherein a first lens group and a second lens group are disposed between the fluorescence detection cell and the fluorescence detector and between the transmission detection cell and the transmission detector, respectively;
signal energy reaching the fluorescence detector and the transmission detector is boosted by the first lens group and the second lens group.
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| CN201920931645.5U CN210953803U (en) | 2019-06-19 | 2019-06-19 | Ultraviolet visible fluorescence spectrometer |
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| CN201920931645.5U CN210953803U (en) | 2019-06-19 | 2019-06-19 | Ultraviolet visible fluorescence spectrometer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110346317A (en) * | 2019-06-19 | 2019-10-18 | 浙江欧文光谱科技有限公司 | UV, visible light Fluorescence Spectrometer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110346317A (en) * | 2019-06-19 | 2019-10-18 | 浙江欧文光谱科技有限公司 | UV, visible light Fluorescence Spectrometer |
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Granted publication date: 20200707 Termination date: 20210619 |