CN210935014U

CN210935014U - Chip reaction box

Info

Publication number: CN210935014U
Application number: CN201921161202.9U
Authority: CN
Inventors: 李全伟; 郑晖
Original assignee: Hangzhou Yuanhe Biotechnology Co ltd
Current assignee: Hangzhou Yuanhe Biotechnology Co ltd
Priority date: 2019-07-23
Filing date: 2019-07-23
Publication date: 2020-07-07
Anticipated expiration: 2029-07-23

Abstract

The utility model provides a chip reaction box, carry box body and baffle including the chip, wherein, the chip carries the box body to be fixed on the baffle and with baffle direct contact, the upper surface that the box body was carried to the chip is provided with the intercommunicating pore, the chip carry the box body with baffle direct contact's lower surface is provided with the recess, the first part of recess is inlayed and is had the chip, and the intercommunicating pore with the recess is linked together. The chip reaction box has good sealing performance, corrosion resistance and high temperature resistance, can realize multi-step circulating reaction, can perform various biochemical reactions, and has the advantages of low price, simple structure, and concise, convenient and safe use.

Description

Chip reaction box

Technical Field

The utility model relates to the field of biotechnology, more specifically relates to a chip reaction box.

Background

The biochip integrates biochemical analysis process on the surface of the biochip according to the principle of specific interaction between biomolecules, thereby realizing high-flux rapid detection of DNA, RNA, polypeptide, protein and other biological components. Biochips can be classified into gene chips, protein chips, cell chips, and tissue chips according to the biomaterial solidified on the chip. A gene chip, also called DNA chip or DNA microarray, is prepared by immobilizing cDNA or oligonucleotides on a microcarrier in the form of a microarray. Protein chips are obtained by immobilizing some non-nucleic acid living matter such as proteins or antigens on a micro-carrier in a microarray system, and probes on the chip are collectively called protein chips in which the target of the protein or chip is a protein. The cell chip is used for fixing cells on a carrier according to a specific mode and detecting the interaction between the cells. The tissue chip is used for the purpose of investigating differences in tissue components such as immunohistochemistry by fixing tissue sections or the like on a carrier in a specific manner.

In recent years, the demands for connection, synthesis and detection of biomacromolecules on biochip surfaces (such as gene chips and protein chips) have become more and more obvious by performing chemical active functional modification on biochip surfaces, and thus the demands for high-quality biochip reaction cassettes have become more and more obvious. However, the existing biochip reaction cartridge is basically a water-phase hybridization reaction cartridge, and mainly has the following problems: 1. the volume is large, the required liquid adding amount is large, and certain cost waste is caused; 2. the sealing performance of the chip carrying box is poor, and the reaction is influenced by external factors such as oxygen, humidity and the like, so that the reaction effect is reduced; 3. the replacement of the reaction reagent can not be realized, so as to meet the requirement 4 of multi-step cyclic reaction, and the corrosion resistance and organic solvent tolerance are not realized.

With the development of society, the development of biological, chemical and medical technologies is accelerated, and the number of biochip experiments performed every year is increased, and various corrosive acid-base reagents and organic reagents are often used, and test conditions of high temperature and closed environment are required, so that the quality requirement of the biochip reaction box is increased, and thus, the research of a high-quality biochip reaction box is urgently needed.

SUMMERY OF THE UTILITY MODEL

To the above-mentioned problem among the prior art, the utility model provides a chip reaction box.

The utility model provides a chip reaction box, carry box body and baffle including the chip, wherein, the chip carries the box body to be fixed on the baffle and with baffle direct contact, the upper surface that the box body was carried to the chip is provided with the intercommunicating pore, the chip carry the box body with baffle direct contact's lower surface is provided with the recess, the first part of recess is inlayed and is had the chip, and the intercommunicating pore with the recess is linked together.

In one embodiment, the first portion of the recess has the same shape as the chip.

In one embodiment, the second portion of the recess is hexagonal in shape.

In one embodiment, the second portion of the groove has a further recessed recess around the interior thereof, and a sealing ring is embedded in the recess.

In one embodiment, the second portion of the recess has an unrecessed portion inside that is not further recessed as a reaction chamber.

In one embodiment, the communication holes are respectively provided at two opposite corners of the hexagon, communicating with the reaction chamber.

In one embodiment, the upper diameter of the communication hole is larger than the lower diameter of the communication hole.

In one embodiment, the number of the communication holes is two, and the communication holes are respectively connected with the liquid inlet and outlet connector and the liquid blowing connector.

In one embodiment, the chip carrier body is made of polytetrafluoroethylene.

In one embodiment, the baffle is a transparent material.

The utility model discloses there is following advantage:

1. the chip is placed in the chip carrying box body comprising the sealing ring, the other end of the chip carrying box body is provided with the baffle, and the chip carrying box body is fixed on the baffle through four screws, so that a sealed environment is realized, the influence of external factors such as oxygen, humidity and the like on the reaction is reduced or even eliminated, and the reaction effect and the correctness are improved;

2. the upper surface of the chip carrying box body is provided with the communicating hole to be connected with the liquid inlet and outlet joint and the liquid blowing joint, liquid inlet and outlet operation in a sealed environment is carried out through the liquid inlet and outlet joint, and residual liquid in the reaction chamber is removed through the liquid blowing joint, so that the replacement of a reaction reagent is realized, the requirement of multi-step cyclic reaction can be met, and the box body does not need to be disassembled or assembled;

3. the reaction cavity is designed to be hexagonal, and the included angle between two sides of the hexagon parallel to the long side of the chip is designed to be as small as possible, so that the dead volume of the reaction solution can be reduced, the reaction solution can be fully reacted and replaced, and the problem of reaction failure caused by pollution of a reaction reagent due to unclean liquid cleaning is avoided;

4. the chip carrying box body is made of polytetrafluoroethylene, can resist corrosion of various acids and alkalis, and can resist organic solvents;

5. the baffle is made of transparent material, such as toughened glass, so that the position of the chip and the liquid addition amount can be observed clearly;

6. the chip reaction box has the advantages of low price, simple structure, and concise, convenient and safe use.

The utility model provides a chip reaction box can be applied to each field that needs utilize the chip to carry out the reaction.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a perspective view showing the structure of a chip reaction cassette according to the present invention.

FIG. 2 is a side sectional view of the chip reaction cassette of the present invention.

FIG. 3 is a top elevational view of the cartridge of the present invention.

Reference numerals: 1-chip carrying box body, 2-baffle, 3-intercommunicating pore, 4-liquid inlet and outlet joint, 5-liquid blowing joint, 6-fixing pore, 7-screw, 8-nut, 9-groove, 10-chip, 11-sealing ring, 12-reaction chamber.

Detailed Description

The technical solution of the present invention will be described clearly and completely with reference to the embodiments of the present invention and the accompanying drawings, and obviously, the described embodiments are only a part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art all belong to the protection scope of the present invention.

Referring to fig. 1-3, the utility model provides a chip reaction box, carry box body 1 and baffle 2 including the chip, wherein, the chip carries box body 1 to fix on the baffle 2 and with 2 direct contact of baffle, the upper surface that the box body 1 was carried to the chip is provided with intercommunicating pore 3, the chip carry box body 1 with the lower surface of 2 direct contact of baffle is provided with recess 9, the first part of recess 9 is inlayed and is had chip 10, and intercommunicating pore 3 with recess 9 is linked together.

In one embodiment, the chip carrying box body 1 is fixed on the baffle 2 and is in direct contact with the baffle 2, the lower surface of the chip carrying box body 1 in direct contact with the baffle 2 is provided with a groove 9, a chip 10 is embedded in a first part of the groove 9, the chip 10 can be fixed through the groove 9, further fixation of the chip 10 can be realized through the baffle 2, and the chip 10 is prevented from falling off.

Further, the first portion of the recess 9 and the chip 10 have the same shape, so that the chip 10 is completely embedded in the first portion of the recess 9. Preferably, the first portion of the recess 9 and the chip 10 are both rectangular in shape.

Further, the depth of the first portion of the recess 9 is the same as the thickness of the chip 10, so that not only can the chip 10 be completely inserted into the recess 9, but also the outflow of the reaction reagent can be prevented.

Further, as shown in FIG. 3, the second portion of the groove 9 has a hexagonal shape, so that the dead volume of the reaction solution can be reduced to facilitate the discharge of the solution. In one embodiment, the opposite parallel sides of the hexagon are parallel to the opposite sides of the chip, and the angle between the parallel sides of the hexagon is designed to be as small as possible to reduce the dead volume of the reaction solution.

Further, when the first portion of the groove 9 is rectangular, the opposite parallel two sides of the hexagon have substantially the same length as the long sides of the rectangle, so that the reaction chamber 12 in the second portion of the groove 9 has a larger contact area with the chip 10 embedded in the first portion of the groove 9, thereby improving the reaction efficiency.

Further, the inner periphery of the second part of the groove 9 is provided with a further recessed concave part, and a sealing ring 11 is embedded in the recessed part. The inner periphery of the second part of the groove 9 is recessed along the periphery to obtain a recessed part, so that the recessed part has the same shape as the second part of the groove 9. The sealing ring 11 is then fitted into the recess to achieve a good seal of the reaction chamber 12 and the chip 10 to prevent the reaction solution from flowing out. The material of the sealing ring 11 is any material known to those skilled in the art that can realize a sealing function, and the present invention is not limited thereto.

Further, the part inside the second part of the groove 9 that is not recessed further serves as a reaction chamber 12, and therefore the reaction chamber 12 also has the same shape as the recessed part and the seal ring 11.

Further, the wire diameter of the sealing ring 11 is larger than the sum of the depth of the recessed portion and the depth of the non-recessed portion inside the second portion of the groove 9 to achieve a good seal.

Further, the upper surface of the cartridge body 1 is provided with communication holes 3, and the communication holes 3 are provided at the corners between the opposite parallel sides of the hexagon, correspond to the chips 10, and communicate with the reaction chamber 12 to introduce or discharge the reaction liquid into or from the reaction chamber 12. Preferably, as shown in fig. 3, the communication holes 3 are provided at the corners between the opposite parallel sides of the hexagon to sufficiently introduce and discharge the reaction solution into and from the reaction chamber, reducing the dead volume of the reaction solution.

Further, the quantity of intercommunicating pore 3 is two to connect with advancing flowing back joint 4 and blowing liquid joint 5 respectively and be connected, set up intercommunicating pore 3 through the upper surface that carries box body 1 at the chip, connect into flowing back and connect and change reaction solution, connect through blowing liquid and connect and clear away the interior raffinate of reaction chamber, thereby carry out the feed liquor and the flowing back operation in the airtight environment, realize the change of reactant, and then make the requirement that can reach the multistep circulation reaction, and need not the dismouting box body.

Further, the liquid inlet and outlet connector 4 and the liquid blowing connector 5 are polyether ether ketone (PEEK) connectors.

Furthermore, the diameter of the upper part of the communication hole 3 is larger than that of the lower part of the communication hole 3, wherein the upper part of the communication hole 3 is mainly used for being connected with the liquid inlet and outlet joint 4 and the liquid blowing and outlet joint 5, the lower part of the communication hole 3 is mainly used for leading the reaction reagent into or out of the reaction chamber 12, and the narrower diameter of the lower part can further reduce the contact of the reaction reagent with the external environment, thereby further reducing the influence of external factors such as oxygen, humidity and the like on the reaction and improving the reaction effect and the correctness.

Further, fixing holes 6 penetrating through the chip carrying box body 1 and the baffle 2 for fixing the chip carrying box body 1 and the baffle 2 are arranged on the chip carrying box body 1 and the baffle 2.

Further, the quantity of fixed orifices 6 is four, and these four fixed orifices 6 set up respectively and carry four angles of box body 1 at the chip to and the relevant position department on the baffle 2, can run through chip and carry box body 1 and baffle 2, thereby realize that the chip carries box body 1 and baffle 2 reliably fixed.

Further, the fixing hole 6 is a threaded hole, and the chip carrying box body 1 and the baffle 2 are reliably fixed through a screw 7 and a nut 8.

Further, the chip carrier body 1 may be made of various materials, such as teflon, which can resist corrosion of various acids and alkalis and can resist organic solvents.

Further, the barrier 2 is made of a transparent material, for example, tempered glass, so that the position of the chip 10 and the amount of liquid added can be clearly observed.

The utility model provides a defect that chip reaction box exists among the prior art, the utility model provides a sealing performance is good, corrosion-resistant, high temperature resistant, and can realize multistep cyclic reaction and carry out various biochemical reactions's chip reaction box. The utility model realizes a sealed environment by placing the chip in the chip carrying box body with the sealing ring, arranging the baffle at the other end and fixing the chip carrying box body on the baffle through four screws, thereby reducing or even eliminating the influence of external factors such as oxygen, humidity and the like on the reaction and improving the reaction effect and the correctness; the upper surface of the chip carrying box body is provided with the communicating hole to be connected with the liquid inlet and outlet joint and the liquid blowing joint, liquid inlet and outlet operation in a sealed environment is carried out through the liquid inlet and outlet joint, and residual liquid in the reaction chamber is removed through the liquid blowing joint, so that the replacement of a reaction reagent is realized, the requirement of multi-step cyclic reaction can be met, and the box body does not need to be disassembled or assembled; the reaction cavity is designed into a hexagon, so that the dead volume of the reaction solution can be reduced, the full reaction and replacement of the reaction solution are facilitated, and the problem of reaction failure caused by pollution of a reaction reagent due to unclean liquid cleaning is avoided; the chip carrying box body is made of polytetrafluoroethylene, can resist corrosion of various acids and alkalis, and can resist organic solvents; the baffle is made of toughened glass, so that the position of the chip and the liquid addition amount can be observed clearly; and the chip reaction box has the advantages of low price, simple structure, and concise, convenient and safe use.

The above embodiments are merely preferred examples of the present invention, which should not be construed as limiting the scope of the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The chip reaction box is characterized by comprising a chip loading box body and a baffle, wherein the chip loading box body is fixed on the baffle and is in direct contact with the baffle, a communicating hole is formed in the upper surface of the chip loading box body, a groove is formed in the lower surface of the chip loading box body, which is in direct contact with the baffle, a chip is embedded in the first part of the groove, and the communicating hole is communicated with the groove.

2. The cartridge of claim 1, wherein the first portion of the recess has the same shape as the chip.

3. The cartridge of any one of claims 1-2, wherein the second portion of the recess has a hexagonal shape.

4. The cartridge of claim 3, wherein a recess is further formed around the inside of the second portion of the groove, and a sealing ring is inserted into the recess.

5. The cartridge of claim 3, wherein the second portion of the recess has an unrecessed portion inside which is not further recessed as a reaction chamber.

6. The cartridge of claim 5, wherein the communication holes are respectively provided at two opposite corners of the hexagon to communicate with the reaction chamber.

7. The cartridge of claim 1, wherein an upper diameter of the communication hole is larger than a lower diameter of the communication hole.

8. The chip reaction cassette according to claim 1, wherein the number of the communication holes is two, and the two communication holes are connected to the liquid inlet and outlet connector and the liquid blowing connector, respectively.

9. The cartridge of claim 1, wherein the cartridge body is made of teflon.

10. The cartridge of claim 1, wherein the baffle is a transparent material.