CN210929354U - Ultra-low temperature cryopreservation container for fish sperm - Google Patents

Abstract

The utility model discloses a fish sperm ultra-low temperature freezes deposits container, including keeping the board and preserving the lid, the center of keeping the board is equipped with the hollow layer, and the upper end and the lower extreme of keeping the board are equipped with upper shed and under shed respectively, and upper shed and under shed all are linked together with the hollow layer, and the upper shed covers the phase-match with preserving, and the under shed packing has the cotton fibre that thoughtlessly seals the powder, and the thickness of preserving the board is 2 ~ 3mm, and the thickness ratio of hollow layer preserves the board thickness and is 0.3 ~ 0.32mm less. The utility model discloses a to freeze the container design and be ultra-thin platelike for the sperm of preserving in the plate body can be heated evenly, and acquire the temperature rapidly in the short time, avoided defects such as the heated is inhomogeneous that cylindrical structural design brought, when putting simultaneously on the frame stifling, can reduce area occupied. In addition, the detachable preservation cover is simple to operate and can be reused.

Description

Ultra-low temperature cryopreservation container for fish sperm

Technical Field

The utility model relates to a sperm low temperature freezes deposits field, concretely relates to roe ultra-low temperature freezes deposits container.

Background

In the process of ultra-low temperature freezing of the sperms, the sperms and preservation solution which are prepared with the antifreeze agent and the diluent agent are required to be filled into a container to be put into an instrument for cooling or to be put into liquid nitrogen for ultra-low temperature preservation, and the containers of the device which are commonly used in the prior production comprise an ampoule bottle, an EP tube and a wheat tubule. Most laboratories and scholars commonly use the wheat tubules at present, and the wheat tubules have the advantages that the wheat tubules are slender, so that the temperature is relatively uniformly and rapidly heated in the cooling process, the preservation effect is relatively good, and the survival rate of thawed sperms is relatively high; however, the straw tube is a cylindrical tube body, so that the straw tube is inconvenient to take and place when being put in a liquid nitrogen tank for fumigation or storage, and is difficult to store in a large capacity in production. The ampoule bottle is relatively troublesome to unfreeze and is not easy to operate, so the ampoule bottle is rarely used in production. The EP tube is thick and heavy, and is difficult to be heated uniformly in a short time, and the preservation effect is obviously reduced.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects of the prior art and providing a fish sperm ultra-low temperature cryopreservation container.

In order to achieve the above object, the utility model adopts the following technical scheme:

a roe ultra-low temperature cryopreservation container comprises a preservation plate and a preservation cover, wherein a hollow layer is arranged at the center of the preservation plate, an upper opening and a lower opening are respectively arranged at the upper end and the lower end of the preservation plate, the upper opening and the lower opening are communicated with the hollow layer, the upper opening is matched with the preservation cover, cotton wool mixed with sealing powder is filled in the lower opening, the thickness of the preservation plate is 2-3 mm, the thickness of the hollow layer is 0.3-0.32 mm smaller than that of the preservation plate, half of the difference between the thickness of the hollow layer and the thickness of the preservation plate is the distance between a sample preserved by the preservation plate and external liquid nitrogen, when the difference is too small, the preserved sample is better heated, but the preservation plate is easy to deform, and the production cost and difficulty can be increased; when the difference is too large, the temperature condition of the sample is affected, so that the difference of the thicknesses is 0.3-0.32 mm, namely the distance between the sample stored in the storage plate and external liquid nitrogen is 0.15-0.16 mm, which is reasonable.

Preferably, the outer wall of the storage plate is further provided with a capacity mark, so that the volume of the sample can be visually and conveniently observed.

Preferably, the outer wall of the storage plate is further provided with a lifting lug.

Preferably, the preservation plate and the preservation cover are both made of polyethylene material.

Preferably, the thickness of the preservation plate is 2.5 mm.

Preferably, the hollow layer has a capacity of 10 mL.

Preferably, the width of the preservation plate is 40-45 mm, and the length of the preservation plate is 150-200 mm. As the storage plate is generally required to be placed in the liquid nitrogen tank, the caliber of the existing liquid nitrogen tank is generally 50mm, and the height is 420mm at least, the width and the length of the storage plate are designed to be 40-45 mm and 150-200 mm more reasonably.

Preferably, the width of the preservation plate is 42mm, and the length is 161 mm.

The utility model has the advantages that: the utility model provides a roe ultra-low temperature freezes deposits container will freeze and deposit the container design for ultra-thin platelike for the sperm of preserving in the plate body receives the temperature more even, and acquires the temperature rapidly in the short time, has avoided receiving defects such as temperature unevenness that cylindrical structure design brought, puts on the frame when stifling simultaneously, can reduce area occupied. In addition, the detachable preservation cover is simple to operate and can be reused.

Drawings

FIG. 1 is a schematic structural view of a roe cryopreservation container of the present invention;

FIG. 2 is a front view of the plate for storing roe in ultra-low temperature cryopreservation container of the present invention;

fig. 3 is a schematic top view of the storage plate of the ultra-low temperature freezing wheat tubule clamping plate device of the present invention.

Detailed Description

The conception and the technical effects of the present invention will be clearly and completely described below with reference to the embodiments, so as to fully understand the objects, aspects and effects of the present invention. It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict.

Example 1:

the utility model provides a protamine ultra-low temperature cryopreserving container, as shown in fig. 1-3, including preserving board 100 and preserving lid 200, preserve board 100 and preserving lid 200 and make by the polyethylene material, the length of preserving board 100 is 161mm, the width is 42mm, the center of preserving board 100 is equipped with hollow layer 300, the thickness of preserving board 100 is 2.5mm, the thickness of hollow layer 300 is 2.2mm, the upper end and the lower extreme of preserving board 100 are equipped with upper shed 400 and under shed 500 respectively, upper shed 400 and under shed 500 all are linked together with hollow layer 300, upper shed 400 and preserving lid 200 phase-match, under shed 500 is filled with the cotton fibre that mixes the sealing powder, still be equipped with capacity sign 600 and lug 700 on the outer wall of preserving board 100.

The use process comprises the following steps: after taking off and preserving lid 200, add the sperm liquid that needs to preserve through upper shed 400, when the filling is packed into liquid, the air that has existed in the inboard can be discharged away through the cotton fibre, after the water of liquid the inside met the sealing powder of cotton fibre the inside, automatic caking has played the confined effect, has prevented that the liquid nitrogen from getting into the inboard and preventing the inboard liquid outflow, has covered after adding and has preserved lid 200, can put into liquid nitrogen tank stifling or save after the rope is tied to the lug 700.

Example 2:

a sperm cryopreservation vessel, compared with example 1, comprises a storage plate 100 with a length of 180mm and a width of 45mm, and is used in the same way as in example 1.

Claims (8)

1. The utility model provides a fish sperm ultra-low temperature freezes deposits container, its characterized in that, is including keeping the board and preserving the lid, the center of keeping the board is equipped with the hollow layer, the upper end and the lower extreme of keeping the board are equipped with upper shed and under shed respectively, the upper shed with the under shed all is linked together with the hollow layer, the upper shed with keep the lid phase-match, the under shed packing has the cotton fibre that thoughtlessly seals the powder, the thickness of keeping the board is 2 ~ 3mm, the thickness of hollow layer compares the little 0.3 ~ 0.32mm of thickness of keeping the board.

2. The container of claim 1, wherein the outer wall of the retention panel is further provided with a volume indicator.

3. The container according to claim 1, wherein the outer wall of the storage plate is further provided with a lifting lug.

4. The container of claim 1, wherein the retention panel and the retention cover are both made of a polyethylene material.

5. The container of claim 1, wherein the holding plate has a thickness of 2.5 mm.

6. The container of claim 1, wherein the hollow layer has a capacity of 10 mL.

7. The container according to claim 1, wherein the width of the retaining plate is 40 to 45mm and the length thereof is 150 to 200 mm.

8. The container of claim 7, wherein the retention panel has a width of 42mm and a length of 161 mm.

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