CN210883161U

CN210883161U - Quantitative PCR kit with high sensitivity and good specificity

Info

Publication number: CN210883161U
Application number: CN201921933142.8U
Authority: CN
Inventors: 徐岩
Original assignee: Nanjing Bairuidi Biotechnology Co ltd
Current assignee: Nanjing Bairuidi Biotechnology Co ltd
Priority date: 2019-11-11
Filing date: 2019-11-11
Publication date: 2020-06-30
Anticipated expiration: 2029-11-11

Abstract

The utility model discloses a sensitivity is high, quantitative PCR kit that specificity is good, including the kit box body, the inboard lower part symmetry fixed mounting of kit box body has the gag lever post, the inboard middle part symmetry fixed mounting of kit box body has the connecting block, the equal fixed mounting in both sides of installation piece has the rectangular block, the jack has been seted up to the upper surface of rectangular block, the inside of every connecting block all is provided with the inserted bar that runs through the jack, the first spring of being connected with the connecting block has been cup jointed in the outside of inserted bar, the common fixedly connected with fixed block of the lower extreme of first spring and inserted bar, the outside of fixed block is rotated and is connected with the link that matches with the gag lever post. The utility model provides a fix through inserted bar and jack between connecting block and the rectangular block, through the pulling inserted bar, make first spring compression, realize inside the inserted bar breaks away from the jack that the rectangular block was seted up to utilize link and gag lever post, the convenience is fixed to the position of inserted bar, is convenient for shake evenly to reagent.

Description

Quantitative PCR kit with high sensitivity and good specificity

Technical Field

The utility model relates to a PCR kit technical field specifically is a quantitative PCR kit that sensitivity is high, the specificity is good.

Background

The real-time quantitative PCR (qPCR) is a technology for realizing quantitative and qualitative analysis of an initial template by detecting a fluorescent signal corresponding to an amplification product of each cycle of the PCR in real time in the PCR amplification process, and the difference between the qPCR technology and the common PCR technology is mainly as follows: the common PCR is more in artificial participation, the data may not be particularly accurate, and agarose electrophoresis detection is needed after the PCR is finished; and qPCR is real-time monitoring and detection, and does not need later-stage electrophoresis, so the qPCR has the advantages of short detection time, simple and convenient operation, high specificity, good repeatability, high sensitivity, high resolution, wide detection range, accurate quantification and the like.

Chinese patent application CN201720395440.0 discloses "a sensitivity is high, quantitative PCR kit that specificity is good", the one end flexible coupling of box body upside has the lid, one side symmetry that the lid is close to the box body is equipped with the inserted sheet, be equipped with the slot that corresponds with the inserted sheet on the lateral wall of box body, be fixed with the support ring on the inside wall of box body, the support ring internal symmetry is equipped with two and places the piece, two one sides of placing the piece mutually on the back of the body all are fixed with the connecting plate, the connecting plate is connected with one side of keeping away from the bottom in the box body through a plurality of locking screw and support ring, be equipped with the swing piece between two connecting plates, the swing piece through the pivot with place the piece and rotate and be connected, the downside fixedly connected with arc pipeline of swing piece, be. Although the utility model discloses a simple structure, convenient to use has prolonged the time of reagent under 2-8 ℃, can shake evenly to reagent simultaneously, has improved work efficiency, but fixes through the locking bolt between connecting plate and the support ring, and inconvenient dismantlement has caused and is difficult to shake even problem to reagent. Therefore, a quantitative PCR kit with high sensitivity and good specificity is provided.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a quantitative PCR kit that sensitivity is high, the specificity is good, solved and fixed through the locking bolt between this utility model's the connecting plate and the support ring, inconvenient dismantlement has caused and has been difficult to shake even problem to reagent.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a high, good quantitative PCR kit of specificity of sensitivity, includes the kit box body, the upper surface of kit box body articulates there is the kit lid, just the inboard lower part symmetry fixed mounting of kit box body has the gag lever post, the inboard middle part symmetry fixed mounting of kit box body has the connecting block, two rotate between the connecting block and be connected with the installation piece, the holding tank has been seted up to the upper surface symmetry of installation piece, just the equal fixed mounting in both sides of installation piece has the rectangular block, the jack has been seted up to the upper surface of rectangular block, every the inside of connecting block all is provided with the inserted bar that runs through the jack, the first spring of being connected with the connecting block has been cup jointed in the outside of inserted bar, the common fixedly connected with fixed block of lower extreme of first spring and inserted bar, the outside of fixed block is rotated and is connected.

Preferably, the upper surface of the kit cover is symmetrically and fixedly provided with a convex block, and a handle is fixedly connected between the two convex blocks.

Preferably, a sealing groove is formed in the upper portion of the inner side of the kit body, and a sealing ring matched with the sealing groove is fixedly mounted on the lower surface of the kit cover.

Preferably, the upper surface of the mounting block is symmetrically provided with placing grooves, and the placing grooves are located on two sides of the containing groove.

Preferably, the movable groove has been seted up to the inboard of holding tank, the mounting groove has been seted up to the inboard symmetry in movable groove, every the equal fixedly connected with second spring in inboard of mounting groove, the one end fixedly connected with of second spring and the splint that the movable groove matches.

Preferably, the splint are arc structures, and two the opposite face of splint is fixed mounting has the slipmat.

Compared with the prior art, the beneficial effects of the utility model are as follows:

1. the utility model provides a fix through inserted bar and jack between connecting block and the rectangular block, through the pulling inserted bar, make first spring compression, realize inside the inserted bar breaks away from the jack that the rectangular block was seted up to utilize link and gag lever post, the convenience is fixed to the position of inserted bar, is convenient for shake evenly to reagent.

2. The utility model discloses there is splint through second spring coupling in the inboard of holding tank, utilizes the deformability of second spring, and the convenience is fixed the reagent pipe of different model specifications, and the practicality is stronger.

Drawings

Fig. 1 is a schematic structural view of the present invention;

fig. 2 is a top view of the mounting block of the present invention;

fig. 3 is a partial enlarged view of part a of the present invention.

In the figure: 1. a kit body; 2. mounting blocks; 3. a limiting rod; 4. a fixed block; 5. connecting blocks; 6. a sealing groove; 7. a kit cover; 8. a seal ring; 9. a bump; 10. a grip; 11. a placement groove; 12. inserting a rod; 13. a first spring; 14. a rectangular block; 15. a jack; 16. a splint; 17. a movable groove; 18. accommodating grooves; 19. mounting grooves; 20. a second spring; 21. and (5) hanging a ring.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Example 1:

referring to fig. 1-3, a high sensitivity and good specificity quantitative PCR kit comprises a kit body 1, a kit cover 7 hinged to the upper surface of the kit body 1, and the lower part of the inner side of the kit body 1 is symmetrically and fixedly provided with a limiting rod 3, the middle part of the inner side of the kit body 1 is symmetrically and fixedly provided with connecting blocks 5, an installation block 2 is rotatably connected between the two connecting blocks 5, the upper surface of the installation block 2 is symmetrically provided with accommodating grooves 18, and the equal fixed mounting in both sides of installation piece 2 has rectangular block 14, and jack 15 has been seted up to rectangular block 14's upper surface, and the inside of every connecting block 5 all is provided with inserted bar 12 that runs through jack 15, and the first spring 13 of being connected with connecting block 5 has been cup jointed in the outside of inserted bar 12, and the common fixedly connected with fixed block 4 of the lower extreme of first spring 13 and inserted bar 12, and the outside of fixed block 4 is rotated and is connected with the link 21 that matches with gag lever post 3.

Referring to fig. 1, the upper surface of the reagent box cover 7 is symmetrically and fixedly provided with bumps 9, and a handle 10 is fixedly connected between the two bumps 9, so that the reagent box can be conveniently lifted.

Referring to fig. 1, a sealing groove 6 is formed in the upper portion of the inner side of the reagent kit case body 1, and a sealing ring 8 matched with the sealing groove 6 is fixedly installed on the lower surface of the reagent kit case cover 7, so that the sealing performance of the connection between the reagent kit case body 1 and the reagent kit cover 7 is improved.

Referring to fig. 1, the mounting block 2 has symmetrically disposed placing grooves 11 on the upper surface thereof, the placing grooves 11 are disposed on two sides of the receiving groove 18, and ice bags are placed on the placing grooves 11, so as to maintain the temperature inside the reagent kit.

Referring to fig. 2-3, the inner side of the accommodating groove 18 is provided with a movable groove 17, the inner side of the movable groove 17 is symmetrically provided with mounting grooves 19, the inner side of each mounting groove 19 is fixedly connected with a second spring 20, and one end of each second spring 20 is fixedly connected with a clamping plate 16 matched with the movable groove 17, so that reagent tubes of different models and specifications can be conveniently fixed.

Referring to fig. 2, the clamping plates 16 are arc-shaped, and anti-slip pads are fixedly mounted on opposite surfaces of the two clamping plates 16, so as to increase the friction force between the clamping plates 16 and the reagent tubes.

The working principle is as follows: during the use, at first open kit lid 7, put into the ice bag to the inside of standing groove 11, a temperature for maintaining 1 inside of kit box body, then put into the inside of holding tank 18 with the reagent pipe, the reagent pipe causes the extrusion to splint 16 this moment, second spring 20 is compressed and shortens, pull fixed block 4 downwards at last, 21 covers the link in the fixed block 4 outside in the outside of gag lever post 3, the inserted bar 12 of being connected with fixed block 4 breaks away from inside the jack 15 that rectangular block 14 seted up, rock installation piece 2, make reagent shaken evenly.

The control mode of the present application is automatically controlled by a controller, the control circuit of the controller can be realized by simple programming by those skilled in the art, and the present application is common knowledge in the field, and the present application mainly protects the mechanical device, so the control mode and the circuit connection are not explained in detail in the present application.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a high, good quantitative PCR kit of specificity of sensitivity, includes kit box body (1), its characterized in that: the upper surface of the kit box body (1) is hinged with a kit box cover (7), the lower part of the inner side of the kit box body (1) is symmetrically and fixedly provided with a limiting rod (3), the middle part of the inner side of the kit box body (1) is symmetrically and fixedly provided with connecting blocks (5), an installing block (2) is rotatably connected between the two connecting blocks (5), the upper surface of the installing block (2) is symmetrically provided with an accommodating groove (18), the two sides of the installing block (2) are both fixedly provided with rectangular blocks (14), the upper surface of each rectangular block (14) is provided with an inserting rod (12) which penetrates through the inserting hole (15), the outer side of each inserting rod (12) is sleeved with a first spring (13) connected with the connecting block (5), the lower ends of the first spring (13) and the inserting rod (12) are fixedly connected with a fixing block (4) together, the outer side of the fixed block (4) is rotatably connected with a hanging ring (21) matched with the limiting rod (3).

2. The quantitative PCR kit with high sensitivity and good specificity according to claim 1, wherein: the upper surface of the kit cover (7) is symmetrically and fixedly provided with convex blocks (9), and a handle (10) is fixedly connected between the two convex blocks (9).

3. The quantitative PCR kit with high sensitivity and good specificity according to claim 1, wherein: the upper portion of the inner side of the kit box body (1) is provided with a sealing groove (6), and the lower surface of the kit box cover (7) is fixedly provided with a sealing ring (8) matched with the sealing groove (6).

4. The quantitative PCR kit with high sensitivity and good specificity according to claim 1, wherein: the mounting block is characterized in that placing grooves (11) are symmetrically formed in the upper surface of the mounting block (2), and the placing grooves (11) are located on two sides of the containing groove (18).

5. The quantitative PCR kit with high sensitivity and good specificity according to claim 1, wherein: activity groove (17) have been seted up to the inboard of holding tank (18), mounting groove (19), every have been seted up to the inboard symmetry in activity groove (17) the inboard equal fixedly connected with second spring (20) of mounting groove (19), splint (16) that the one end fixedly connected with of second spring (20) matches with activity groove (17).

6. The quantitative PCR kit with high sensitivity and good specificity according to claim 5, wherein: the clamping plates (16) are of arc-shaped structures, and anti-skid pads are fixedly mounted on opposite surfaces of the two clamping plates (16).