CN210855920U

CN210855920U - High-efficient purification device of gene recombination albumin

Info

Publication number: CN210855920U
Application number: CN201921287804.9U
Authority: CN
Inventors: 齐智; 艾云鹏; 柏凯; 高重
Original assignee: Shandong Jiantong Biotechnology Co ltd
Current assignee: Shandong Jiantong Biotechnology Co ltd
Priority date: 2019-08-09
Filing date: 2019-08-09
Publication date: 2020-06-26
Anticipated expiration: 2029-08-09

Abstract

The utility model relates to a protein purification technical field specifically is a high-efficient purification device of gene recombination albumin, including the mounting bracket, top one side of mounting bracket is equipped with quick-witted case, and top one side of mounting bracket is equipped with the processing case, the bottom fixed mounting liquid feed pump of mounting bracket, and the inside of machine case is equipped with the stock solution chamber, and the bottom in stock solution chamber is equipped with out the liquid pipeline, and beneficial effect is: the utility model discloses top at the gravity post is provided with handles the case, the inside of handling the case is provided with the agitator and gives vent to anger the pipeline, wherein the agitator is used for stirring protein magma, and the pipeline of giving vent to anger bubbles in to the magma through the outlet vent, can know according to protein biology characteristic, suitable stirring and vibrations, can be when keeping protein biology activity, release protein from magma or solution state, consequently, handle the case and be equivalent to the preprocessing device of protein purification, can accelerate albumin to appear in the gravity post, the efficiency of purification is improved.

Description

High-efficient purification device of gene recombination albumin

Technical Field

The utility model relates to a protein purification technical field specifically is a high-efficient purification device of gene recombination albumin.

Background

Albumin is the most important protein in human plasma and is used to maintain the nutrition and osmotic pressure of the body, and in the field of biology, in order to obtain pure albumin and maintain the biological activity of protein, the impurities in the protein protoplasm need to be removed, i.e. purification. The structure of the existing protein purification device is more simplified, the existing protein purification device is mainly lack of a pretreatment mechanism, the time required by purification is longer, the whole purification efficiency is not high, and therefore, the production of an efficient albumin purification device is a technical problem which must be solved by technicians in the industry, therefore, the utility model provides a high-efficiency purification device for gene recombination albumin is used for solving the problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a high-efficient purification device of gene recombination albumin to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a high-efficiency purification device for gene recombinant albumin comprises a mounting rack, wherein a case is arranged on one side of the top of the mounting rack, a processing box is arranged on one side of the top end of the mounting rack, a liquid supply pump is fixedly installed at the bottom end of the mounting rack, a liquid storage cavity is arranged in the case, a liquid outlet pipeline is arranged at the bottom end of the liquid storage cavity, one end, far away from the liquid storage cavity, of the liquid outlet pipeline is connected with a liquid inlet of the liquid supply pump, a liquid supply pipe is connected onto a liquid outlet of the liquid supply pump, one end, far away from the liquid supply pump, of the liquid supply pipe is connected with the processing box, gravity columns are arranged below the bottom end of the processing box, the number of the gravity columns is not lower than two, the gravity columns are fixedly connected onto the mounting rack through pipe clamps, liquid inlet channels are arranged at the top ends of the gravity columns, liquid outlet channels are arranged, and valves are arranged on the liquid inlet channel and the liquid outlet channel.

Preferably, the inside top of handling the case is equipped with the agitator, and the outside one side of handling the case is equipped with miniature servo motor, the one end of agitator is rotated and is connected on the inner wall of handling the case, and the other end of agitator runs through and handles the case and be connected with miniature servo motor's output shaft.

Preferably, the top of handling the case is equipped with the gas outlet, is equipped with the admission line below the inside of handling the case, the admission line level sets up, its one end and the inner wall fixed connection who handles the case, and its other end runs through the lateral wall of handling the case and extends to the outside of handling the case.

Preferably, the top side of inlet duct evenly is equipped with the pipeline of giving vent to anger, the top side of the pipeline of giving vent to anger evenly runs through and has seted up the venthole, and communicates between pipeline of giving vent to anger and the inlet duct, welded fastening between the two.

Preferably, the bottom of handling the case is equipped with and supplies liquid branch pipe, the quantity that supplies liquid branch pipe equals with the quantity of gravity post, and supplies the bottom of liquid branch pipe all to be connected with inlet channel's top.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model discloses top at the gravity post is provided with handles the case, the inside of handling the case is provided with the agitator and gives vent to anger the pipeline, wherein the agitator is used for stirring protein magma, and the pipeline of giving vent to anger bubbles in to the magma through the outlet vent, can know according to protein biology characteristic, suitable stirring and vibrations, can be when keeping protein biology activity, release protein from magma or solution state, consequently, handle the case and be equivalent to the preprocessing device of protein purification, can accelerate albumin to appear in the gravity post, the efficiency of purification is improved.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a sectional view of the structure of the treatment tank of the present invention;

fig. 3 is a schematic view of the structure of the air inlet pipe of the present invention.

In the figure: the device comprises a mounting frame 1, a case 2, a processing box 3, a gravity column 4, a liquid supply pump 5, a liquid supply pipe 6, a liquid outlet pipe 7, a valve 8, a stirrer 9, a miniature servo motor 10, a gas outlet 11, a gas inlet pipeline 12, a liquid supply branch pipe 13, a gas outlet pipeline 14 and a gas outlet hole 15.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-3, the present invention provides a technical solution: the utility model provides a high-efficient purification device of gene recombination albumin, including mounting bracket 1, top one side of mounting bracket 1 is equipped with quick-witted case 2, top one side of mounting bracket 1 is equipped with handles case 3, bottom fixed mounting liquid feed pump 5 of mounting bracket 1, the inside of machine case 2 is equipped with the stock solution chamber, the bottom in stock solution chamber is equipped with the drain pipe, the one end that the stock solution chamber was kept away from to the drain pipe is connected with the inlet of feed pump 5, it is equipped with feed pipe 6 to connect on the liquid outlet of feed pump 5, feed pipe 6 keeps away from the one end of feed pump 5 and handles case 3 and connect, the stock solution chamber is used for saving protein magma, feed pump 5 can be with the protein magma pump in the stock solution chamber of quick-witted case 2 go into handle case 3 in.

Handle the bottom below of case 3 and be equipped with gravity post 4, the quantity of gravity post 4 is not less than two, and gravity post 4 all is through pipe strap fixed connection on mounting bracket 1, the top of gravity post 4 all is equipped with inlet channel, the bottom of gravity post 4 all is equipped with liquid outlet channel, liquid inlet channel's top is all connected on handling case 3, liquid outlet pipe 7 is connected jointly to liquid outlet channel's bottom, and all be provided with the valve on inlet channel and the liquid outlet channel, a liquid outlet pipe 7 is connected with a plurality of liquid outlet channel simultaneously promptly, as shown in fig. 1, gravity post 4 is used for albumin to appear the purification, and the upper and lower both ends of gravity post 4 all are provided with the valve.

The inside top of handling case 3 is equipped with agitator 9, and the outside one side of handling case 3 is equipped with miniature servo motor 10, and the one end of agitator 9 is rotated and is connected on the inner wall of handling case 3, and the other end of agitator 9 runs through and handles case 3 and with miniature servo motor 10's output shaft, and miniature servo motor 10 can drive agitator 9 rotatory, and the rate of rotation is invariable, as shown in fig. 2.

The top of handling case 3 is equipped with gas outlet 11, and the inside below of handling case 3 is equipped with inlet duct 12, and inlet duct 12 level sets up, its one end and the inner wall fixed connection who handles case 3, and its other end runs through the lateral wall of handling case 3 and extends to the outside of handling case 3, and inlet duct 12 runs through the lateral wall of handling case 3, and the department of just running through is through sealed processing.

The top side of the air inlet pipeline 12 is uniformly provided with air outlet pipelines 14, the top side of the air outlet pipeline 14 is uniformly penetrated and provided with air outlet holes 15, the air outlet pipeline 14 is communicated with the air inlet pipeline 12 and is welded and fixed with the air inlet pipeline 12, the position relation between the air inlet pipeline 12 and the air outlet pipeline 14 is shown in figure 3, and air in the air inlet pipeline 12 enters the air outlet pipeline 14 and is finally discharged from the air outlet holes 15.

The bottom end of the treatment box 3 is provided with liquid supply branch pipes 13, the number of the liquid supply branch pipes 13 is equal to that of the gravity columns 4, and the bottom ends of the liquid supply branch pipes 13 are connected with the top end of the liquid inlet channel.

The working principle is as follows: the utility model discloses during the use, through opening liquid feed pump 5, go into the processing case 3 with the protein magma pump in the stock solution chamber, in processing case 3 rethread confession liquid branch pipe 13 discharged into gravity column 4 with the magma, albumin was extracted by the purification in 4 inside precipitations of gravity column, discharges in the drain pipe 7 at last. Wherein the processing box 3 is equivalent to a protein pretreatment device, the inside of the processing box 3 is provided with a stirrer 9 and an air inlet pipeline 12, wherein the stirrer 9 is connected with an external micro servo motor 10, and the protein raw pulp in the processing box 3 can be stirred after rotating. An air outlet pipeline 14 is arranged above the air inlet pipeline 12, the number of the air outlet pipelines 14 is a plurality, and an air outlet hole 15 is formed in the top side of the air outlet pipeline 14 in a penetrating mode. The outer end of the air inlet pipe 12 is connected with an air supply device, so that air enters the air outlet pipe 14 through the air inlet pipe 12 and is finally sprayed out of the air outlet hole 15, and the vibration effect can be achieved. According to the biological characteristics of the protein, the proper stirring and vibration can release the protein from the primary pulp or the dissolved state while maintaining the biological activity of the protein, thereby being beneficial to the precipitation of the protein in the gravity column and accelerating the purification efficiency.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a high-efficient purification device of gene recombination albumin, includes mounting bracket (1), its characterized in that: the device is characterized in that a case (2) is arranged on one side of the top of the mounting frame (1), a treatment box (3) is arranged on one side of the top of the mounting frame (1), a liquid supply pump (5) is fixedly mounted at the bottom of the mounting frame (1), a liquid storage cavity is arranged in the case (2), a liquid outlet pipeline is arranged at the bottom of the liquid storage cavity, one end of the liquid outlet pipeline, far away from the liquid storage cavity, is connected with a liquid inlet of the liquid supply pump (5), a liquid supply pipe (6) is connected onto a liquid outlet of the liquid supply pump (5), one end of the liquid supply pipe (6), far away from the liquid supply pump (5), is connected with the treatment box (3), gravity columns (4) are arranged below the bottom of the treatment box (3), the number of the gravity columns (4) is not lower than two, the gravity columns (4) are fixedly connected onto the mounting frame (1) through pipe clamps, liquid inlet channels are arranged at the top ends of the gravity, the top of feed liquor channel all connects on handling case (3), drain pipe (7) are connected jointly to the bottom of liquid outlet channel, and all are provided with the valve on feed liquor channel and the liquid outlet channel.

2. The device for efficiently purifying gene recombinant albumin as claimed in claim 1, wherein: handle the inside top of case (3) and be equipped with agitator (9), the outside one side of handling case (3) is equipped with miniature servo motor (10), the one end of agitator (9) is rotated and is connected on the inner wall of handling case (3), and the other end of agitator (9) runs through and handles case (3) and with the output shaft of miniature servo motor (10).

3. The device for efficiently purifying gene recombinant albumin as claimed in claim 1, wherein: the top of handling case (3) is equipped with gas outlet (11), and the inside below of handling case (3) is equipped with air intake pipe (12), air intake pipe (12) level sets up, its one end and the inner wall fixed connection who handles case (3), and its other end runs through the lateral wall of handling case (3) and extends to the outside of handling case (3).

4. The device for efficiently purifying gene recombinant albumin as claimed in claim 3, wherein: the top side of inlet line (12) evenly is equipped with outlet pipe way (14), outlet hole (15) have evenly been seted up in the top side of outlet pipe way (14) even running through, and communicate between outlet pipe way (14) and inlet pipe way (12), welded fastening between the two.

5. The device for efficiently purifying gene recombinant albumin as claimed in claim 1, wherein: the bottom of handling case (3) is equipped with and supplies liquid branch pipe (13), the quantity that supplies liquid branch pipe (13) and the quantity of gravity post (4) equals, and the bottom that supplies liquid branch pipe (13) all is connected with inlet channel's top.