CN210796463U - Cell blows broken membrane device - Google Patents
Cell blows broken membrane device Download PDFInfo
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- CN210796463U CN210796463U CN201921395459.0U CN201921395459U CN210796463U CN 210796463 U CN210796463 U CN 210796463U CN 201921395459 U CN201921395459 U CN 201921395459U CN 210796463 U CN210796463 U CN 210796463U
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Abstract
The utility model provides a cell blowing and membrane breaking device, which belongs to the technical field of biological inspection equipment and comprises a suction pipe, wherein the top end of the suction pipe is provided with a suction pipe head, and the inner bottom end of the suction pipe is provided with a one-way air valve; the top end of the straw head is provided with a main tube, the top end of the main tube is provided with an air bag, and the air bag is connected with the button and the straw head through a gas pipe; the top of gasbag is installed and is pressed the rifle head, and the flute profile through-hole has been seted up to outer wall one side of main part pipe, and the bottom of flute profile through-hole links to each other with the one end of gas-supply pipe, and cylinder and spring assembly have been placed to the inner chamber of flute profile through-hole, form air intake mechanism. The cell blowing and breaking membrane device can prevent the whole straw pipeline from being polluted by adopting the arc-shaped straw head; through the straw that uses 1ml to and the use of cooperation button, gas-supply pipe and gasbag etc. utilize the air to blow the mode that one blows and inhale and combine appropriate dynamics to blow and beat the cell, in order to reach the purpose of destroying the cell membrane, not only more accurate, labour saving and time saving moreover more.
Description
Technical Field
The utility model belongs to the technical field of biological test equipment technique and specifically relates to a cell blows broken membrane device.
Background
In present biological cell detects, extract the chromosome and need carry out rupture of membranes to the cell and handle, blow through external force and beat promptly and make the cell membrane rupture, release the cell nucleus in it, this in-process, to blowing and beating the dynamics requirement higher, if the dynamics is too big, injure the cell nucleus easily, if the dynamics is undersize, then can't rupture the cell membrane, present cell rupture of membranes' mode is: manually holding the disposable suction tube by hand, extending the suction tube mouth into the cell fluid of the test tube, extruding the suction tube head to blow air through the suction tube, striking the blown air onto the cell membrane to break the cell membrane, then lifting out the suction tube, sucking air in the recovery process of the suction tube head, then extending the suction tube mouth with the sucked air into the cell fluid of the test tube to blow air, and repeating the steps in such a way, wherein in the process, the blowing frequency and the blowing force need to depend on manual experience, so that time and labor are wasted, the precision control is poor, and the cell membrane rupture quality is poor; meanwhile, the suction pipe is not protected when entering and exiting the test tube, so that the pollution of cell sap is easily caused, the inspection progress and the inspection result are easily influenced, and the cell blowing and membrane breaking device is designed to solve the problems.
Disclosure of Invention
The utility model aims at providing a cell blows broken membrane device to the above-mentioned problem that prior art exists. The specific technical scheme is as follows:
a cell blowing and membrane breaking device comprises a suction pipe, wherein a suction pipe head is installed at the top end of the suction pipe, a one-way air valve is installed at the bottom end inside the suction pipe, a main pipe is installed at the top end of the suction pipe head, an air bag is installed at the top end of the main pipe, the air bag is connected with a button and the suction pipe head through a gas pipe, a pressing gun head is installed at the top end of the air bag, a groove-shaped through hole is formed in one side of the outer wall of the main pipe, the bottom end of the groove-shaped through hole is connected with one end of the gas pipe, a cylinder is placed in the inner cavity of the groove-shaped through hole, the top end of the cylinder is connected with the button, the bottom end of the cylinder extends into the inner cavity of the gas pipe connected with the; a baffle is arranged at one side of the middle part of the outer wall of the cylinder, which is close to the button, and the baffle is positioned at the inner side of the groove-shaped through hole; the spring is sleeved on the outer wall of the cylinder between the baffle plate and the rubber mat, the diameter of the spring is larger than the edge diameter of the groove-shaped through hole and is adaptive to the maximum diameter inside the groove-shaped through hole, and the spring is clamped inside the groove-shaped through hole in a compression state. .
Furthermore, the shape of the gas pipe is T-shaped, and three gas ports of the gas pipe are respectively connected with the pipette head, the air bag and the groove-shaped through hole.
Furthermore, the top of the pressing gun head is arc-shaped and is connected with the top end of the air bag in a sealing manner.
Further, the pipette head is an arc head.
Further, the diameter of the arc-shaped pipette head is larger than that of the test tube opening.
Further, the size of the straw is 1 ml.
Furthermore, the one-way air valve is a miniature one-way air valve, and the outer side wall of the one-way air valve is fixedly connected with the inner side wall of the suction pipe in a sealing manner.
Further, the one-way gas valve can only blow gas outwards.
Compared with the prior art, the beneficial effects of the utility model are that: the cell blowing and breaking membrane device can prevent the whole straw pipeline from being polluted by adopting the arc-shaped straw head; through the straw that uses 1ml to and the cooperation button, the use of gas-supply pipe and gasbag etc. utilizes the air one to blow the dynamics of beating that the mode of inhaling combines 1ml gas to blow the cell membrane, in order to reach the purpose of destroying the cell membrane, not only the dynamics is more even, accurate more, guarantees the intact of cell nucleus when can effectively blow the cell membrane, and labour saving and time saving all has apparent improvement from rupture of membranes quality and rupture of membranes efficiency moreover.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic structural diagram of the button of the present invention.
In the figure: 1. the suction tube comprises a suction tube body 2, a suction tube head 3, a one-way air valve 4, a main tube 5, an air delivery pipe 6, a button 7, an air bag 8, a pressing gun head 9, a cylinder 10, a spring 11, a groove-shaped through hole 12, a rubber cushion 13 and a baffle plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
As shown in figure 1-2, a cell blows and breaks membrane device, including straw 1, the specification of straw 1 is 1ml, straw head 2 is installed on the top of straw 1, straw head 2 is the arc head, just the diameter of arc straw head 2 is greater than the diameter of the test tube mouth, has avoided the contaminated condition of whole body to take place, and the inside bottom of straw 1 installs one-way air valve 3, one-way air valve 3 is miniature one-way air valve, and its lateral wall is sealed fixed connection with the straw 1 inside wall, and one-way air valve 3 can only blow off gas outwards, can not suck back liquid, has increased the precision, and main body pipe 4 is installed on the top of straw head 2, and gasbag 7 is installed on the top of main body pipe 4, and gasbag 7 is connected through gas-supply pipe 5 with button 6, straw head 2 through gas-supply pipe 5, and the shape of gas-supply pipe 5 is "T" font, and three gas ports of gas-, The air bag 7 and the groove-shaped through hole 11, the pressing gun head 8 is installed at the top end of the air bag 7, the top of the pressing gun head 8 is arc-shaped and is hermetically connected with the top end of the air bag 7, the groove-shaped through hole 11 is formed in one side of the outer wall of the main body pipe 4,
the bottom end of the groove-shaped through hole 11 is connected with one end of the gas pipe 5, a cylinder 9 is placed in the inner cavity of the groove-shaped through hole 11, the top end of the cylinder 9 is connected with the button 6, the bottom end of the cylinder 9 extends into the inner cavity of the gas pipe 5 connected with the groove-shaped through hole 11, a rubber cushion 12 is installed at the bottom end of the cylinder 9, and the rubber cushion 12 is located outside the groove-shaped through hole 11 and has a diameter larger than that of the groove-shaped through hole 11;
a baffle 13 is arranged at one side of the middle part of the outer wall of the cylinder 9 close to the button 6, and the baffle 13 is positioned at the inner side of the groove-shaped through hole 11; the outer wall of the cylinder 9 between the baffle 13 and the rubber cushion 12 is sleeved with a spring 10, the spring 10 is a compression spring, the elastic coefficient range is 0.1-3/cm, and the spring with the corresponding coefficient can be selected according to specific requirements; the diameter of the spring 10 is larger than the edge diameter of the groove-shaped through hole 11 and is adapted to the maximum diameter inside the groove-shaped through hole 11, the spring 10 is clamped inside the groove-shaped through hole 11 in a compressed state, and the compression elasticity of the spring enables the rubber gasket 12 to be sealed with the main pipe 4 in a normal state. The cell blowing and breaking device has the advantages that all structures are connected perfectly and in a sealed state, and the conditions of air leakage, liquid leakage and the like cannot be generated.
The detailed connection means is a known technology in the field, and the following main introduction working principle and process specifically work as follows: when the cell liquid detector is used, a suction tube is firstly inserted into cell liquid to be detected, a pressing gun head 8 is pressed downwards, the pressing gun head 8 presses an air bag 7 to extrude air to enter an inner cavity of a suction tube head 2 through an air delivery pipe 5, then the air enters a suction tube 1 through an inner cavity of the suction tube head 2, then the air is discharged out of the suction tube 1 through a one-way air valve 3 to blow cell membranes, then a button 6 is pressed towards the inner cavity of a main tube 4 to push a cylinder 9, the cylinder 9 moves inwards to extrude a spring 10, the spring 10 contracts to push a rubber pad 12 to be separated from the inner wall of the main tube 4, a gap is formed between the rubber pad and a groove-shaped through hole 11, the air enters the inner cavity of the air bag 7 under the action of suction force of the air bag 7, the air bag 7 is recovered, then the button 6 is released, the rubber pad 12 is driven by the spring 10 to be attached to the, so circulate, can accomplish under the condition of not pulling out the straw 1 and accomplish the continuous pressing and blow and beat the cell, and the dynamics is even, accurate, labour saving and time saving.
Other technical features than those described in the specification are known to those skilled in the art.
In the above description of the present invention, it should be understood that the terms "upper", "lower", "high", "low", "top", "bottom", "inner", "outer", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship that the product of the present invention is conventionally placed when in use, or the orientation or positional relationship that one skilled in the art would conventionally understand, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the device or element that is referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as limiting the present invention.
The foregoing is considered as illustrative only of certain exemplary embodiments of this invention, and is therefore to be understood that the drawings and descriptions will be regarded as illustrative in nature and not as restrictive. It should also be noted that, for those skilled in the art, a plurality of improvements and additions can be made in various ways without departing from the principles of the present invention, and these improvements and additions should also be considered as the protection scope of the present invention.
Claims (8)
1. A cell blows broken membrane device which characterized in that: comprises a straw (1), a straw head (2) is installed on the top end of the straw (1), a one-way air valve (3) is installed at the inner bottom end of the straw (1), a main body pipe (4) is installed on the top end of the straw head (2), an air bag (7) is installed on the top end of the main body pipe (4), the air bag (7) is connected with a button (6) and the straw head (2) through an air pipe (5), a pressing gun head (8) is installed on the top end of the air bag (7), a groove-shaped through hole (11) is formed in one side of the outer wall of the main body pipe (4), the bottom end of the groove-shaped through hole (11) is connected with one end of the air pipe (5), a cylinder (9) is placed in the inner cavity of the groove-shaped through hole (11), the top end of the cylinder (9) is connected with the button (6), and the bottom end of the cylinder (, the bottom end of the cylinder (9) is provided with a rubber pad (12), and the rubber pad (12) is positioned outside the groove-shaped through hole (11) and has a diameter larger than that of the groove-shaped through hole (11); a baffle (13) is arranged on one side of the middle part of the outer wall of the cylinder (9) close to the button (6), and the baffle (13) is positioned on the inner side of the groove-shaped through hole (11); the outer wall of the cylinder (9) between the baffle (13) and the rubber cushion (12) is sleeved with a spring (10), the diameter of the spring (10) is larger than the edge diameter of the groove-shaped through hole (11) and is adapted to the maximum diameter inside the groove-shaped through hole (11), and the spring (10) is clamped inside the groove-shaped through hole (11) in a compressed state.
2. The cell rupture membrane device of claim 1, wherein: the air delivery pipe (5) is T-shaped, and three air ports of the air delivery pipe (5) are respectively connected with the pipette head (2), the air bag (7) and the groove-shaped through hole (11).
3. The cell rupture membrane device of claim 1, wherein: the top of the pressing gun head (8) is arc-shaped and is connected with the top end of the air bag (7) in a sealing way.
4. The cell rupture membrane device of claim 1, wherein: the pipette head (2) is an arc-shaped head.
5. The cell rupture membrane device of claim 4, wherein: the diameter of the arc-shaped pipette head (2) is larger than that of the test tube opening.
6. The cell rupture membrane device of claim 1, wherein: the specification of the straw (1) is 1 ml.
7. The cell rupture membrane device of claim 1, wherein: the one-way air valve (3) is a miniature one-way air valve, and the outer side wall of the one-way air valve is fixedly connected with the inner side wall of the suction pipe (1) in a sealing way.
8. The cell rupture membrane device of claim 1, wherein: the one-way air valve (3) can only blow air outwards.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201921395459.0U CN210796463U (en) | 2019-08-26 | 2019-08-26 | Cell blows broken membrane device |
Applications Claiming Priority (1)
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CN201921395459.0U CN210796463U (en) | 2019-08-26 | 2019-08-26 | Cell blows broken membrane device |
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CN210796463U true CN210796463U (en) | 2020-06-19 |
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CN201921395459.0U Active CN210796463U (en) | 2019-08-26 | 2019-08-26 | Cell blows broken membrane device |
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2019
- 2019-08-26 CN CN201921395459.0U patent/CN210796463U/en active Active
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