CN210683812U - Nucleic acid extraction and purification device - Google Patents
Nucleic acid extraction and purification device Download PDFInfo
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- CN210683812U CN210683812U CN201921272798.XU CN201921272798U CN210683812U CN 210683812 U CN210683812 U CN 210683812U CN 201921272798 U CN201921272798 U CN 201921272798U CN 210683812 U CN210683812 U CN 210683812U
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Abstract
The utility model discloses a nucleic acid extraction and purification device, the power distribution box comprises a box body, the top outer wall of box is provided with the protective housing, and the top inner wall of protective housing is provided with the motor, motor output shaft's one end outer wall is provided with the rotation post, and the both sides outer wall of rotation post all is provided with the puddler, the top outer wall of box is opened there is the fixed orifices, and pegs graft in the fixed orifices and have into the liquid fill, one side outer wall of box is provided with temperature sensor, one side outer wall of box is provided with control panel, and the inside treater that is provided with of control panel, one side outer wall of box is provided with the sampler barrel, and pegs graft and have the pull pole at the. The utility model discloses a motor drives and rotates post and puddler, can accelerate the precipitation speed of protein, has satisfied staff's the demand of drawing, can also carry out the control by temperature change work to the box in, avoids forming the protein production of sediment and decomposes, influences the purity of drawing nucleic acid.
Description
Technical Field
The utility model relates to a nucleic acid extraction and purification technical field especially relates to a nucleic acid extraction and purification device.
Background
Nucleic acid extraction and purification are the basis of molecular biological tests, and the nucleic acid purification method is the most important factor influencing the quality of extracted nucleic acid and is also the key of success or failure of downstream molecular biological tests. The common methods for purifying nucleic acid at present comprise a PC extraction method, a high-salt precipitation method, a centrifugal column method and a biological magnetic bead method, and the methods have advantages and disadvantages respectively.
Generally, a high-pressure precipitation nucleic acid extraction and purification device does not have a temperature control function, so that protein cannot reach the optimal precipitation temperature, residues are easily formed, the purity of nucleic acid extracted by people is affected, and the use requirements of people cannot be met. Meanwhile, the traditional nucleic acid extraction and purification device is often slow in purification rate, and the work efficiency of people is reduced. Therefore, a nucleic acid extraction and purification device is needed to solve the above problems.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the defects existing in the prior art and providing a nucleic acid extraction and purification device.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model provides a nucleic acid extraction purification device, includes the box, the top outer wall of box is provided with the protective housing, and the top inner wall of protective housing is provided with the motor, the one end outer wall of motor output shaft is provided with rotates the post, and rotates the both sides outer wall of post and all be provided with the puddler, the top outer wall of box is opened has the fixed orifices, and pegs graft in the fixed orifices and have into liquid fill, one side outer wall of box is provided with temperature sensor.
As the utility model discloses scheme further again, one side outer wall of box is provided with control panel, and the inside treater that is provided with of control panel.
As the utility model discloses scheme further again, one side outer wall of box is provided with the sampler barrel, and pegs graft and have the pull pole the top outer wall of sampler barrel, and the one end outer wall of pull pole is provided with the piston, and peg graft and have the sampling tube the bottom outer wall of sampler barrel, and the one end of sampling tube extends to inside the box.
As the utility model discloses scheme further again, the bottom outer wall of box is pegged graft and is had the fluid-discharge tube, and one side outer wall of fluid-discharge tube is provided with the control valve, and one side outer wall of fluid-discharge tube is pegged graft and is had first filter screen.
As the utility model discloses scheme further again, one side outer wall of box is provided with the refrigerator body, and the top outer wall of refrigerator body is pegged graft and is had the air duct, and one side outer wall of air duct is provided with the solenoid valve, and the one end of air duct extends to inside the box.
As the utility model discloses scheme further again, the both sides inner wall of box all is provided with the filter mantle, and one side outer wall of filter mantle opens and has logical groove, leads to the inner wall all around of groove and all is provided with the second filter screen.
The utility model has the advantages that:
1. through the arranged motor, the rotating column and the stirring rod, the motor drives the rotating column and the stirring rod to rotate, so that the precipitation rate of protein can be increased, the working efficiency of people is improved, and the extraction requirements of workers are met;
2. through the arranged refrigerator body, the temperature sensor and the control panel, when the temperature sensor detects that the temperature in the box body is not within the rated range, an electric signal is transmitted to the control panel through a wire, the control panel controls the refrigerator body to work, the temperature control work is carried out in the box body, and the situation that the decomposition of protein forming precipitates influences the purity of extracted nucleic acid is avoided;
3. through the sampling tube, filter mantle and the pull pole that set up, people twitch the pull pole and pass through the piston and make the interior nucleic acid solution suction sampling tube with the box of negative pressure that produces of sampling tube in, made things convenient for staff's sample detection, the second filter screen of filter mantle one side outer wall setting can avoid protein to deposit and cause the jam to the connecting tube simultaneously.
Drawings
FIG. 1 is a schematic view of the nucleic acid isolation and purification apparatus according to example 1;
FIG. 2 is a schematic perspective view of the nucleic acid purification apparatus according to example 1;
FIG. 3 is a schematic sectional view of the nucleic acid isolation and purification apparatus according to example 2.
In the figure: 1 box, 2 control panel, 3 refrigerator bodies, 4 air ducts, 5 protective casings, 6 liquid inlet hoppers, 7 temperature sensors, 8 pull rods, 9 sampling cylinders, 10 sampling tubes, 11 first filter screens, 12 liquid discharge pipes, 13 rotating columns, 14 stirring rods and 15 filter covers.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
Reference will now be made in detail to embodiments of the present patent, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present patent and are not to be construed as limiting the present patent.
In the description of this patent, it is to be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the patent and for the simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the patent.
In the description of this patent, it is noted that unless otherwise specifically stated or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and can include, for example, fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meaning of the above terms in this patent may be understood by those of ordinary skill in the art as appropriate.
Example 1
Referring to fig. 1-2, a nucleic acid extraction and purification device, including box 1, there is protective housing 5 box 1's top outer wall through bolted connection, and there is the motor protective housing 5's top inner wall through bolted connection, the welding of the one end outer wall of motor output shaft has rotation post 13, and the both sides outer wall of rotation post 13 all has puddler 14 through bolted connection, the top outer wall of box 1 is opened has the fixed orifices, and it has into liquid fill 6 to peg graft in the fixed orifices, there is temperature sensor 7 box 1's one side outer wall through bolted connection.
Wherein, the outer wall of one side of the box body 1 is connected with a control panel 2 through a bolt, a processor is arranged in the control panel 2, the type of the processor is ARM9TDMI, the outer wall of one side of the box body 1 is connected with a sampling tube 9 through a bolt, and the outer wall of the top of the sampling tube 9 is inserted with a pull rod 8, the outer wall of one end of the pull rod 8 is provided with a piston, the outer wall of the bottom of the sampling tube 9 is inserted with a sampling tube 10, one end of the sampling tube 10 extends to the interior of the box body 1, the outer wall of the bottom of the box body 1 is inserted with a liquid discharge tube 12, and the outer wall of one side of the liquid discharge pipe 12 is connected with a control valve through a bolt, the outer wall of one side of the liquid discharge pipe 12 is inserted with a first filter screen 11, the outer wall of one side of the box body 1 is connected with a refrigerator body 3 through a bolt, and the top outer wall of refrigerator body 3 is pegged graft and is had air duct 4, and one side outer wall of air duct 4 is provided with the solenoid valve, and the one end of air duct 4 extends to inside the box 1.
The working principle is as follows: during the use, the staff is through advancing liquid bucket 6 with the high salt reagent, starter motor, the motor drives rotation post 13 and puddler 14 and rotates, can accelerate protein's precipitation rate, people's work efficiency has been improved, staff's the demand of drawing has been satisfied, temperature sensor 7 detects when the temperature in box 1 is not in rated range, give control panel 2 with electric signal transmission through the wire, control panel 2 control refrigerator body 3 work, to carrying out temperature control work in the box 1, avoid forming the protein production of sediment and decompose, the purity of nucleic acid is drawed in the influence, the in-process of work is drawed to nucleic acid to the device, people twitch pull pole 8 and produce the negative pressure through the piston in making the sampler barrel 9 and draw in with the nucleic acid solution in the box 1 sampling barrel 9, staff's sample detection has been made things convenient for.
Example 2
Referring to fig. 3, a nucleic acid extraction and purification device, this embodiment compares with embodiment 1, and the both sides inner wall of box 1 all has filter mantle 15 through bolted connection, and the outer wall of one side of filter mantle 15 is opened has logical groove, and the inner wall all has the second filter screen through bolted connection all around of logical groove.
The working principle is as follows: when in use, a worker passes the high-salt reagent through the liquid inlet hopper 6, the motor is started, the motor drives the rotating column 13 and the stirring rod 14 to rotate, can accelerate the precipitation rate of protein, improve the working efficiency of people, meet the extraction requirement of workers, when the temperature sensor 7 detects that the temperature in the box body 1 is not in the rated range, the electric signal is transmitted to the control panel 2 through a lead, the control panel 2 controls the refrigerator body 3 to work, the temperature control work is carried out in the box body 1, the protein forming the precipitate is prevented from being decomposed and influencing the purity of the extracted nucleic acid, in the process of extracting nucleic acid by the device, people can draw the drawing rod 8 to generate negative pressure in the sampling cylinder 9 through the piston so as to draw the nucleic acid solution in the box body 1 into the sampling cylinder 9, thereby facilitating the sampling detection of workers, meanwhile, the second filter screen arranged on the outer wall of one side of the filter cover 15 can prevent protein precipitation from blocking the connecting pipeline.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.
Claims (6)
1. The utility model provides a nucleic acid extraction purification device, includes box (1), its characterized in that, the top outer wall of box (1) is provided with protective housing (5), and the top inner wall of protective housing (5) is provided with the motor, the one end outer wall of motor output shaft is provided with rotates post (13), and rotates the both sides outer wall of post (13) and all is provided with puddler (14), the top outer wall of box (1) is opened has the fixed orifices, and pegs graft in the fixed orifices and have into liquid fill (6), one side outer wall of box (1) is provided with temperature sensor (7).
2. The nucleic acid extraction and purification device according to claim 1, wherein a control panel (2) is disposed on an outer wall of one side of the box body (1), and a processor is disposed inside the control panel (2).
3. The nucleic acid extraction and purification device according to claim 1, wherein a sampling cylinder (9) is arranged on the outer wall of one side of the box body (1), a pull rod (8) is inserted into the outer wall of the top of the sampling cylinder (9), a piston is arranged on the outer wall of one end of the pull rod (8), a sampling tube (10) is inserted into the outer wall of the bottom of the sampling cylinder (9), and one end of the sampling tube (10) extends into the box body (1).
4. The nucleic acid extraction and purification device according to claim 2, wherein a drain pipe (12) is inserted into the outer wall of the bottom of the box body (1), a control valve is arranged on the outer wall of one side of the drain pipe (12), and a first filter screen (11) is inserted into the outer wall of one side of the drain pipe (12).
5. The nucleic acid extracting and purifying device according to claim 2, wherein a refrigerator body (3) is arranged on the outer wall of one side of the box body (1), the air duct (4) is inserted into the outer wall of the top of the refrigerator body (3), the electromagnetic valve is arranged on the outer wall of one side of the air duct (4), and one end of the air duct (4) extends into the box body (1).
6. The nucleic acid extracting and purifying device according to claim 4 or 5, wherein the inner walls of the two sides of the box body (1) are provided with the filter covers (15), the outer wall of one side of each filter cover (15) is provided with a through groove, and the inner walls of the periphery of the through groove are provided with the second filter screens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921272798.XU CN210683812U (en) | 2019-08-07 | 2019-08-07 | Nucleic acid extraction and purification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921272798.XU CN210683812U (en) | 2019-08-07 | 2019-08-07 | Nucleic acid extraction and purification device |
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| CN210683812U true CN210683812U (en) | 2020-06-05 |
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| CN201921272798.XU Active CN210683812U (en) | 2019-08-07 | 2019-08-07 | Nucleic acid extraction and purification device |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112226338A (en) * | 2020-10-20 | 2021-01-15 | 广州吉源生物科技有限公司 | Nucleic acid extraction and purification device |
| CN112480251A (en) * | 2020-11-30 | 2021-03-12 | 江西中洪博元生物技术有限公司 | Intelligent high-specificity combined VEGFA multi-antibody screening and purifying device |
| CN113648832A (en) * | 2021-09-15 | 2021-11-16 | 氘源(嘉兴)科技有限公司 | Continuous purification device of high-purity deuteration reagent |
| CN114480086A (en) * | 2022-02-18 | 2022-05-13 | 西安文理学院 | Nucleic acid extraction and purification device and working method thereof |
| WO2023151185A1 (en) * | 2022-02-08 | 2023-08-17 | 深圳潘道医学检验实验室 | Automatic nucleic acid extractor |
-
2019
- 2019-08-07 CN CN201921272798.XU patent/CN210683812U/en active Active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112226338A (en) * | 2020-10-20 | 2021-01-15 | 广州吉源生物科技有限公司 | Nucleic acid extraction and purification device |
| CN112480251A (en) * | 2020-11-30 | 2021-03-12 | 江西中洪博元生物技术有限公司 | Intelligent high-specificity combined VEGFA multi-antibody screening and purifying device |
| CN112480251B (en) * | 2020-11-30 | 2023-07-11 | 江西中洪博元生物技术有限公司 | Intelligent high-specificity combined VEGFA multi-antibody screening and purifying device |
| CN113648832A (en) * | 2021-09-15 | 2021-11-16 | 氘源(嘉兴)科技有限公司 | Continuous purification device of high-purity deuteration reagent |
| WO2023151185A1 (en) * | 2022-02-08 | 2023-08-17 | 深圳潘道医学检验实验室 | Automatic nucleic acid extractor |
| CN114480086A (en) * | 2022-02-18 | 2022-05-13 | 西安文理学院 | Nucleic acid extraction and purification device and working method thereof |
| CN114480086B (en) * | 2022-02-18 | 2023-07-07 | 西安文理学院 | Nucleic acid extraction and purification device and working method thereof |
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