CN210675123U

CN210675123U - Disk chip magnetic particle moving device and detection device

Info

Publication number: CN210675123U
Application number: CN201921450609.3U
Authority: CN
Inventors: 顾志鹏; 李达; 张意如; 焦政; 陈跃东
Original assignee: Dongguan HEC Tech R&D Co Ltd
Current assignee: Dongguan HEC Tech R&D Co Ltd
Priority date: 2019-08-30
Filing date: 2019-08-30
Publication date: 2020-06-05
Anticipated expiration: 2029-08-30

Abstract

The utility model provides a pair of disc chip magnetism particle mobile device and detection device relates to medical detection equipment technical field, include: a disk chip having at least one reaction chamber; the disc chip is arranged on the centrifugal control mechanism so as to be capable of rotating along the central axis of the disc chip in a dead axle manner under the driving of the centrifugal control mechanism; the radial control mechanism comprises a driving assembly and at least one guide rail assembly; the guide track of the guide rail assembly is arranged along the radial direction of the disc chip, and the driving assembly is in driving connection with the guide rail assembly; the magnetic part is arranged on the guide rail assembly and driven by the guide rail assembly to reciprocate along the radial direction of the disc chip; the centrifugal control mechanism and the radial control mechanism are relatively fixed. In the technical scheme, the radial control mechanism is matched with the centrifugal control mechanism, and the magnetic particles can be indirectly controlled to continuously move circumferentially and radially on the disc chip through controlling the magnetic part, so that the moving precision of the magnetic particles is improved, and the precision of a detection result is guaranteed.

Description

Disk chip magnetic particle moving device and detection device

Technical Field

The utility model belongs to the technical field of medical treatment check out test set technique and specifically relates to a disc chip magnetic particle mobile device and detection device are related to.

Background

The disc chip has the advantages of good integration performance and simple control, thereby being widely applied to the field of POCT (point of care testing) including biochemistry, immunity and molecular diagnosis. In addition, the disc chip can realize high-throughput reaction and detection processes, and further promotes the application of the disc chip in the fields of diagnosis and the like. Currently, in the diagnostic field, most reaction systems involve the use and manipulation of magnetic particles, and the precise control of the magnetic particles directly affects the transfer efficiency and transfer consistency of the magnetic particles, and then the detection precision.

In the prior art, when a disc chip is used for detection, the control precision of the movement of magnetic particles is low, synchronous high-precision movement of the magnetic particles of a plurality of reaction cavities cannot be realized, particularly high-flux centripetal movement of the magnetic particles is necessary for complex biochemical reaction on the disc chip, and a detection result with high precision cannot be obtained. Therefore, it is always difficult in the art how to improve the control precision of the magnetic particle movement to improve the precision of the detection result, and how to improve the high flux magnetic particle movement of the disk chip.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a disc chip magnetism particle mobile device and detection device to solve the technical problem that the control accuracy that exists among the prior art to the magnetism particle removal is low, can't realize the synchronous high accuracy of magnetism particle removal of a plurality of reaction cavitys.

The utility model provides a pair of disc chip magnetism particle mobile device, include:

a disk chip having at least one reaction chamber;

the disc chip is arranged on the centrifugal control mechanism and can rotate along the central axis of the disc chip in a fixed axis manner under the driving of the centrifugal control mechanism;

a radial control mechanism comprising a drive assembly and at least one guide rail assembly; the guide track of the guide rail assembly is arranged along the radial direction of the disc chip, and the driving assembly is in driving connection with the guide rail assembly;

the magnetic part is arranged on the guide rail assembly and driven by the guide rail assembly to reciprocate along the radial direction of the disc chip;

the centrifugal control mechanism and the radial control mechanism are relatively fixed.

It is worth to say that, the disc chip of the present invention includes a circular chip, and also includes other chips with a central geometric symmetry shape, as long as it is convenient to perform a centrifugal operation.

Further, the centrifugal control mechanism comprises a first motor, a first rotating shaft and a chip mounting structure;

the driving end of the first motor is in driving connection with one end of the first rotating shaft, and the other end of the first rotating shaft is connected with the chip mounting structure; the disc chip is mounted on the chip mounting structure and is arranged coaxially with the first rotating shaft.

Furthermore, the chip mounting structure comprises a mounting disc and a fixing shaft arranged along the central axis of the mounting disc, the mounting disc is in transmission connection with the first rotating shaft, the central axes of the mounting disc and the first rotating shaft are overlapped, and a central shaft hole of the disc chip is in sleeve assembly with the fixing shaft;

the disk chip is positioned opposite to the mounting disk.

Furthermore, at least one positioning column is arranged on the mounting disc, and a positioning hole corresponding to the positioning column is arranged on the disc chip;

the disc chip and the mounting disc are oppositely positioned through the positioning column and the positioning hole.

Furthermore, the side wall of the fixed shaft is provided with at least one positioning bulge, and the inner hole wall of the central shaft hole is provided with a positioning groove corresponding to the positioning bulge;

the disc chip and the mounting disc are oppositely positioned through the positioning bulge and the positioning groove.

Further, the reaction chamber comprises a first reaction cavity, a second reaction cavity and a third reaction cavity which are arranged along the circumferential direction of the disk chip;

the first reaction cavity is communicated with the second reaction cavity through a first transfer channel, and the second reaction cavity is communicated with the third reaction cavity through a second transfer channel; the first reaction cavity, the second reaction cavity and the third reaction cavity are all provided with holes.

Further, the guide rail assembly comprises a guide rail and a sliding piece; the sliding part is assembled with the guide rail in a linear sliding mode, the magnetic part is installed on the sliding part, and the driving assembly is connected with the sliding part in a driving mode.

Further, the driving assembly comprises a second motor, a first gear set and at least one rack; the driving end of the second motor is in driving connection with the driving end of the first gear set, and the driven end of the first gear set is in driving connection with the rack;

the rack is connected with the sliding piece.

Further, the first gear set comprises a first driving gear, a first intermediate gear, a second intermediate gear and at least one first driven gear; the first intermediate gear and the second intermediate gear are coaxially and synchronously assembled in a rotating mode, and the highest point of the first intermediate gear is lower than the lowest point of the first driven gear;

the driving end of the second motor is in driving connection with the first intermediate gear through the first driving gear, and the second intermediate gear is in driving connection with the rack through the first driven gear.

Further, the driving assembly comprises a third motor, a second gear set and at least one connecting rod; the driving end of the third motor is in driving connection with the driving end of the second gear set, and the driven end of the second gear set is hinged with the sliding piece through the connecting rod for driving.

Further, the second gear set comprises a second driving gear, a third intermediate gear and a fourth intermediate gear; the third intermediate gear and the fourth intermediate gear are coaxially and synchronously assembled in a rotating mode, and the highest point of the third intermediate gear is lower than the lowest point of the connecting rod;

the driving end of the third motor is in driving connection with the third intermediate gear through the second driving gear, and the fourth intermediate gear is in hinged driving with the sliding piece through the connecting rod.

Further, the driving assembly comprises a fourth motor, a third driving gear, a second driven gear with at least one guide hole and a guide piece matched with the guide hole;

the driving end of the fourth motor is in driving connection with the third driving gear, and the third driving gear is meshed with the second driven gear; the second driven gear and the disc chip are coaxially arranged, the guide hole is arc-shaped, and the distance between the guide hole and the circle center of the second driven gear is gradually reduced in the direction from one end of the guide hole to the other end of the guide hole;

the guide piece is connected with the sliding piece and is in sliding fit with the track of the guide hole, and the sliding piece is driven by the guide piece to reciprocate along the guide rail; the sliding piece is provided with a supporting piece, and the magnetic piece is installed on the supporting piece and located above the second driven gear.

Further, the guide member comprises a roller shaft and a roller assembled on the roller shaft; the roller shaft is mounted on the sliding piece along the axial direction of the second driven gear, and the roller is in rolling fit with the guide hole.

Further, the support member passes through the guide hole.

The utility model also provides a detection device, include disc chip magnetic particle mobile device.

In the technical scheme, the radial control of the magnetic particles is realized by adopting a radial control mechanism consisting of a guide rail assembly and a driving assembly. The driving assembly is used for driving the guide rail assembly to move linearly and indirectly controls the magnetic part to move along the radial direction of the disc chip through the linear control effect of the guide rail assembly, and the moving precision of the magnetic part can be improved in the process of indirect control of the driving assembly in such a way, so that the moving precision of magnetic particles is improved. With it complex, the circumference control to the magnetic particle has adopted centrifugal control mechanism to realize, centrifugal control mechanism can drive the disc chip dead axle and rotate, cooperate the back with radial control mechanism, can be through the continuous circumference and the radial removal of control magnetic particle on the disc chip to the control of magnetic part, the difficult point to magnetic particle circumference and radial movement in succession among the prior art has been overcome, and the precision that the magnetic particle removed has been improved simultaneously, guarantee to the testing result, and can realize the synchronous high accuracy removal of the magnetic particle in a plurality of reacting chambers.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is an assembly view of a mobile device according to an embodiment of the present invention;

FIG. 2 is a diagram of a drive configuration of the mobile device shown in FIG. 1;

fig. 3 is an assembly view of a mobile device according to another embodiment of the present invention;

FIG. 4 is a diagram of a drive structure of the mobile device shown in FIG. 3;

fig. 5 is an assembly view of a mobile device according to another embodiment of the present invention;

FIG. 6 is a diagram of a drive structure of the mobile device shown in FIG. 5;

FIG. 7 is an assembly view of the guide shown in FIG. 5;

fig. 8 is a structural diagram of a centrifugal control mechanism according to an embodiment of the present invention;

fig. 9 is a perspective view of a disk chip according to an embodiment of the present invention;

fig. 10 is a plan view of a disk chip according to an embodiment of the present invention.

Reference numerals:

1. a disk chip; 2. a centrifugal control mechanism; 3. a radial control mechanism;

11. a magnetic member; 12. a reaction chamber;

121. a first reaction chamber; 122. a second reaction chamber;

123. a third reaction chamber; 124. a first transfer channel;

125. a second transfer channel; 126. an aperture; 127. a central shaft hole;

21. a first motor; 22. a first rotating shaft; 23. a chip mounting structure;

231. mounting a disc; 232. a fixed shaft; 233. a positioning column;

234. positioning holes; 235. positioning the projection; 236. positioning a groove;

31. a guide rail assembly; 32. a drive assembly;

311. a guide rail; 312. a slider;

321. a second motor; 322. a first gear set; 323. a rack;

3221. a first drive gear; 3222. a first intermediate gear;

3223. a second intermediate gear; 3224. a first driven gear;

324. a third motor; 325. a second gear set; 326. a connecting rod;

3251. a second driving gear; 3252. a third intermediate gear; 3253. a fourth intermediate gear;

327. a fourth motor; 328. a third driving gear; 329. a second driven gear;

3291. a guide hole; 3292. a guide member; 3293. a support member; 3294. and a roller.

Detailed Description

The technical solution of the present invention will be described clearly and completely with reference to the accompanying drawings, and obviously, the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

As shown in fig. 1 to 10, the present invention provides a moving device for high flux magnetic particles of a disc chip, including: a disc chip 1 having at least one reaction chamber 12;

the disc chip 1 is arranged on the centrifugal control mechanism 2 and can rotate along the central axis of the disc chip 2 in a fixed axis manner under the driving of the centrifugal control mechanism 2;

a radial control mechanism 3, said radial control mechanism 3 comprising a drive assembly 32 and at least one guide rail assembly 31; the guide track of the guide rail assembly 31 is arranged along the radial direction of the disc chip 1, and the driving assembly 32 is in driving connection with the guide rail assembly 31;

a magnetic member 11, wherein the magnetic member 11 is mounted on the guide rail assembly 31 to reciprocate along the radial direction of the disc chip 1 under the driving of the guide rail assembly 31;

the centrifugal control mechanism 2 and the radial control mechanism 3 are relatively fixed.

The utility model discloses a disc chip 1 includes circular chip, also includes other chips that have the centrogeometric symmetrical shape, as long as be convenient for carry on centrifugal operation can. In some embodiments, the disk chip 1 is preferably a circular chip.

In the moving device, the centrifugal control mechanism 2 is used for controlling magnetic particles to move in the radial direction in the reaction chamber 12 of the disc chip 1, the radial control mechanism 3 is used for controlling the magnetic particles to move in the radial direction in the reaction chamber 12 of the disc chip 1, continuous displacement of the magnetic part 11 in the radial direction and the circumferential direction of the disc chip 1 can be realized through the cooperation of the centrifugal control mechanism 2 and the radial control mechanism 3, and the magnetic particles can move in the radial direction and the circumferential direction in the reaction chamber 12 under the driving of the magnetic force of the magnetic part 11. Therefore, in this control method, the accuracy of movement of the magnetic fine particles depends on the accuracy of movement of the magnetic member 11.

When the magnetic part 11 moves in the radial direction, the driving assembly 32 is matched with the guide rail assembly 31, the driving assembly 32 can drive the guide rail assembly 31 to move linearly, the guide rail assembly 31 guides the magnetic part, the magnetic part 11 can move in the reciprocating manner along the radial direction of the disc chip 1, and therefore the magnetic force drives the magnetic particles to move in the reciprocating manner along the radial direction of the disc chip 1.

In this control method, the accuracy of the linear movement of the guide rail assembly 31 is indirectly controlled by adjusting the driving accuracy of the driving assembly 32, thereby controlling the accuracy of the radial movement of the magnetic material 11 and the magnetic fine particles. Therefore, the accuracy of the linear movement of the guide rail assembly 31 can be controlled to improve the accuracy of the radial movement of the magnetic particles.

When the magnetic member 11 moves circumferentially, the rotation of the fixed axis of the disc chip 1 by the centrifugal control mechanism 2 is mainly used for realizing. After the disc chip 1 is installed on the centrifugal control mechanism 2, the centrifugal control mechanism 2 can drive the disc chip 1 to rotate along the central axis of the disc chip 1. When the disk chip 1 rotates in a fixed axis manner, the disk chip 1 and the magnetic member 11 on the guide rail assembly 31 generate relative displacement, that is, the magnetic member 11 is not moved, and the disk chip 1 rotates, so that the magnetic member 11 can move along the circumferential direction of the disk chip 1 relative to the disk chip 1, and the magnetic member 11 drives the magnetic particles in the reaction chamber 12 to rotate along the circumferential direction of the disk chip 1 through magnetic force. In this control method, the accuracy of circumferential movement of the magnetic member 11 and the magnetic fine particles is indirectly controlled by adjusting the rotational accuracy of the disk chip 1. Therefore, the accuracy of the circumferential movement of the magnetic particles can be improved by controlling the accuracy of the circumferential rotation of the disk chip 1.

Therefore, the moving device adopts a mode of actively controlling the movement of the magnetic part 11, indirectly controlling the movement of the magnetic particles by using the magnetic part 11, and transfers the control of the movement precision of the magnetic particles to the linear movement precision of the guide rail component 31 or the circumferential rotation precision of the disc chip 1 by matching the centrifugal control mechanism 2 and the radial control mechanism 3, thereby ensuring that the linear movement precision of the guide rail component 31 or the circumferential rotation precision of the disc chip 1 greatly reduces the technical difficulty. When the magnetic particles are controlled to move, the magnetic particles can move along the radial direction and the circumferential direction of the disc chip 1 under the coordination of the centrifugal control mechanism 2 and the radial control mechanism 3, the radial movement and the circumferential movement of the magnetic particles can be continuously and crossly carried out, and the movement precision can be effectively controlled.

The material of the disc chip 1 can be glass, silicon wafer or common polymer material. The polymer material includes Polydimethylsiloxane (PDMS), polyurethane, epoxy resin, Polymethylmethacrylate (PMMA), Polycarbonate (PC), Cyclic Olefin Copolymer (COC), Polystyrene (PS), Polyethylene (PE), fluoroplastic, and the like. The material of the disc chip 1 made of polymer material can be one or a combination of several of the above materials. The processing method of the disc chip 1 can be determined according to the material and structure, for example, one or more of different methods such as photoetching, numerical control, pouring, injection molding, laser engraving, plasma etching, wet etching and the like are selected to manufacture the disc chip.

The magnetic member 11 may be made of, for example, a strong magnet of rubidium, iron, boron, or the like, and the strength and size of the magnet may be determined according to the amount of magnetic particles and the moving speed. The number of the reaction chambers 12 may be one or more, and when the number is plural, the plural reaction chambers 12 are arranged along the circumferential direction of the disk chip 1, so that the magnetic particles in the plural reaction chambers 12 can be synchronously controlled under the cooperative control of the centrifugal control mechanism 2 and the radial control mechanism 3. Multiple reaction chambers 12 can be used to test the same target of different samples, or different targets of the same sample, thereby improving the detection efficiency, and those skilled in the art can set and use the same according to the needs.

The centrifugal control mechanism 2 and the radial control mechanism 3 are required to be relatively fixed and maintained in relative positions when being matched with each other, so as to ensure the accuracy of the movement of the magnetic member 11 and the relative displacement between the magnetic member 11 and the disk chip 1. The relative fixation of the centrifugal control mechanism 2 and the radial control mechanism 3 can adopt a base, a bracket and other modes, for example, the centrifugal control mechanism 2 and the radial control mechanism 3 are relatively fixed through a common or different bracket so as to keep relative positions in the working process, and the specific structure can be set according to the requirements of volume, portability and the like, and is not limited herein. Meanwhile, in order to ensure the working stability and other detection requirements of the centrifugal control mechanism 2 and the radial control mechanism 3, a damping component, a sample adding component, an incubation component and other detection components can be matched, and the comprehensive function of the mobile device is increased.

Referring to fig. 8, the centrifugal control mechanism 2 includes a first motor 21, a first rotating shaft 22, and a chip mounting structure 23; the driving end of the first motor 21 is in driving connection with one end of the first rotating shaft 22, and the other end of the first rotating shaft 22 is connected with the chip mounting structure 23; the disc chip 1 is mounted on the chip mounting structure 23 coaxially with the first shaft 22.

When the centrifugal control mechanism 2 works, the first motor 21 is controlled to rotate, and after the first motor 21 rotates, the first rotating shaft 22 is driven to rotate in a fixed axis mode through the driving end of the first motor. Since the disk chip 1 is mounted on the chip mounting structure 23 and is coaxial with the first rotating shaft 22, the disk chip 1 is driven by the first rotating shaft 22 to rotate (rotate in the circumferential direction) in a fixed axis. In cooperation with the magnetic force of the magnetic member 11, the magnetic particles can move in the reaction chamber 12 in the circumferential direction under the relative displacement between the two.

The first motor 21 may be a stepping motor or a servo motor, and the stepping motor or the servo motor can ensure a rotation angle during the rotation process. Therefore, according to the requirement of the rotation precision of the magnetic particles, a stepping motor or a servo motor corresponding to the step angle can be selected to realize the control of the movement precision of the magnetic particles. When the driving end of the first motor 21 is connected to the first rotating shaft 22, a shaft coupling, a fixed welding or other connection methods may be adopted for coaxial connection, which is not described herein.

With continued reference to fig. 8, the chip mounting structure 23 includes a mounting plate 231 and a fixing shaft 232 disposed along a central axis of the mounting plate 231, the mounting plate 231 is in transmission connection with the first rotating shaft 22, and the central axes of the mounting plate 231 and the first rotating shaft 22 are coincident, and the central shaft hole 127 of the disk chip 1 is in sleeve fit with the fixing shaft 232; the disk chip 1 is positioned opposite to the mounting plate 231. The magnetic member 11 may be disposed above the disk chip 1 or below the disk chip 1, and in some embodiments, when the magnetic member 11 is disposed below the disk chip 1, the mounting plate 231 and the reaction chamber 12 do not overlap in the axial direction of the disk chip 1.

When the disk chip 1 and the first rotating shaft 22 are installed relatively, the installation disk 231 with the disk structure is adopted, after the disk chip 1 is installed on the installation disk 231 through the fixing shaft 232, only the circumferential part close to the circle center on the disk chip 1 is covered in the axial direction, so that the reaction chamber 12 is conveniently arranged on the portion, far away from the circle center, of the disk chip 1 in the circumferential direction, the reaction chamber 12 is ensured not to be overlapped with the installation disk 231 in the axial direction, and the relative interference with the installation disk 231 is avoided when the magnetic part 11 is controlled to move. Simultaneously, can also be close to the partial circumference of the centre of a circle, even bearing disc chip 1 on disc chip 1, improve bearing area, stability when guaranteeing disc chip 1 to rotate.

When the disk chip 1 and the mounting plate 231 are relatively positioned, the interference fit between the central shaft hole 127 of the disk chip 1 and the fixing shaft 232 can be realized, or other positioning structures can be adopted to realize the positioning.

Preferably, as shown in fig. 8, at least one positioning column 233 is disposed on the mounting plate 231, and a positioning hole 234 corresponding to the positioning column 233 is disposed on the disk chip 1; the disk chip 1 and the mounting disk 231 are positioned opposite to each other through the positioning posts 233 and the positioning holes 234.

After the positioning hole 234 of the disk chip 1 is relatively inserted into the positioning column 233 of the mounting disk 231, the positioning hole 234 and the positioning column 233 can prevent circumferential dislocation between the disk chip 1 and the mounting disk 231, so that the disk chip 1 is synchronously driven to rotate when the mounting disk 231 rotates. One pair or multiple pairs of positioning holes 234 and positioning pillars 233 may be provided, the number of positioning holes 234 and positioning pillars 233 may be optionally selected according to the strength requirement and the arrangement requirement, and the structure may be adapted to the relative positioning between the disk chip 1 and the mounting disk 231 in different embodiments, which is not described herein.

As shown in the embodiment of fig. 3, the sidewall of the fixed shaft 232 is provided with at least one positioning protrusion 235, and the inner hole wall of the central shaft hole 127 is provided with a positioning groove 236 corresponding to the positioning protrusion 235; the disk chip 1 is positioned opposite to the mounting plate 231 by the positioning projection 235 and the positioning groove 236.

After the positioning protrusions 235 on the side wall of the fixing shaft 232 are relatively inserted into the positioning grooves 236 on the inner hole wall of the central shaft hole 127, the circumferential dislocation between the disc chip 1 and the mounting disc 231 can be prevented through the limiting effect between the positioning protrusions 235 and the positioning grooves 236, and the disc chip 1 is synchronously driven to rotate when the mounting disc 231 rotates. One pair or multiple pairs of positioning protrusions 235 and positioning grooves 236 may be provided, the number of positioning protrusions 235 and positioning grooves 236 may be optionally selected according to the strength requirement and the arrangement requirement, and the structure may also be adapted to the relative positioning between the disk chip 1 and the mounting disk 231 in different embodiments, which is not described herein.

Referring to fig. 9 and 10, the reaction chamber 12 includes a first reaction chamber 121, a second reaction chamber 122, and a third reaction chamber 123 disposed along a circumferential direction of the disk chip 1; the first reaction cavity 121 is communicated with the second reaction cavity 122 through a first transfer passage 124, and the second reaction cavity 122 is communicated with the third reaction cavity 123 through a second transfer passage 125; the first reaction cavity 121, the second reaction cavity 122 and the third reaction cavity 123 are all provided with holes 126. The aperture 126 may also serve as a vent.

The reaction chamber 12 can be formed by three reaction chambers, namely a first reaction chamber 121, a second reaction chamber 122 and a third reaction chamber 123, and the structure of the reaction chamber 12 can be particularly suitable for magnetic particle double-antibody sandwich chemiluminescence immunoassay and similar detection work. Here, the operation of the mobile device will be described in detail by taking magnetic particle double antibody sandwich chemiluminescence immunoassay as an example.

In the process of performing the magnetic particle double antibody sandwich chemiluminescence immunoassay by using the mobile device, referring to fig. 10, the magnetic member 11 may be first separated from the disk chip 1, and the sample to be detected, the magnetic particles and the solution may be added into the first reaction chamber 121 through the hole 126 of the first reaction chamber 121. The centrifugal control mechanism 2 is started to enter an oscillation mode, the oscillation mode drives the disc chip 1 to reciprocate along the circumferential direction through the reciprocating rotation of the centrifugal control mechanism 2 in the circumferential direction, the magnetic particles in the first reaction cavity 121 oscillate in the first reaction cavity 121 by using the inertia effect, and the added solution is combined to form a double-antibody sandwich structure which is attached to the magnetic particles. According to the requirement of oscillation, the rotation angle, speed and reciprocating frequency of the centrifugal control mechanism 2 can be set, which is not limited herein.

The oscillation mode is stopped after the double-reactance sandwich structure is formed, the magnetic part 11 is driven by the radial control mechanism 3 to move close to the disc chip 1 in the radial direction of the disc chip 1, and magnetic particles are adsorbed and gathered to the position A at one end, far away from the circle center of the disc chip 1, in the first reaction cavity 121 under the magnetic acting force of the magnetic part 11. And continuously controlling the magnetic part 11 to move, so that the magnetic part 11 drives the magnetic particles to move to the position B along the radial direction of the disk chip 1. Then, the disk chip 1 is rotated by the centrifugal control mechanism 2, and the magnetic particles are driven by the magnetic member 11 to move along the circumferential direction of the disk chip 1 into the first transfer passage 124 through the relative displacement of the magnetic member 11 and the disk chip 1 in the circumferential direction, and enter the second reaction chamber 122 through the first transfer passage 124 to reach the position C. The moving speed of the magnetic member 11 can be adjusted according to the amount of the magnetic particles and the strength of the magnetic force of the magnetic member 11.

After entering the second reaction chamber 122, the radial control mechanism 3 drives the magnetic member 11 to move away from the center of the circle along the radial direction of the disk chip 1, so as to drive the magnetic particles to move from the position C to the position D. At this time, the radial control mechanism 3 is continuously utilized to drive the magnetic member 11 to move radially away from the magnetic particles, so as to ensure that the magnetic particles are no longer controlled by the magnetic force of the magnetic member 11. The cleaning solution is added into the second reaction chamber 122 through the hole 126 of the second reaction chamber 122, the centrifugal control mechanism 2 is started to enter an oscillation mode, the oscillation mode is that the centrifugal control mechanism 2 rotates in a reciprocating manner in the circumferential direction to drive the disk chip 1 to rotate in a reciprocating manner in the circumferential direction, and the magnetic particles in the second reaction chamber 122 oscillate in the second reaction chamber 122 by using the inertia effect, so as to sufficiently clean the magnetic particles.

After the cleaning is completed, the oscillation mode is stopped, the centrifugal control mechanism 2 and the radial control mechanism 3 are started, the magnetic part 11 is driven to move in the radial direction and the circumferential direction of the disc chip 1, so that the magnetic particles are driven to repeat the moving mode in the first reaction cavity 121 and the second reaction cavity 122, the magnetic particles are made to move from the position D to the position E and the position F in the radial direction, enter the third reaction cavity 123 through the second transfer channel 125, and sequentially reach the position G and the position H. At this time, the magnetic member 11 is driven away from the magnetic particles by the radial control mechanism 3, ensuring that the magnetic particles are no longer controlled by the magnetic force of the magnetic member 11. Substrate liquid is added through the hole 126 of the third reaction cavity 123, the oscillation mode is started through the centrifugal control mechanism 2 again, the reaction is completed, and the luminescence detection is carried out, so that the detection process of the magnetic particle double-antibody sandwich chemiluminescence immunoassay is completed.

Besides, the moving device can also be applied to the fields of routine in-vitro diagnosis and biochemical detection and any field requiring disc type micro-fluidic chips and high-flux magnetic particle moving requirements. Those skilled in the art can set the structure of the reaction chamber 12 according to the detection requirements in different fields, for example, the reaction chamber 12 includes reaction cavities with different structures, different numbers, and different arrangements, which is not limited herein.

Preferably, the guide rail assembly 31 includes a guide rail 311 and a slider 312; the sliding member 312 is linearly slidably assembled with the guide rail 311, the magnetic member 11 is mounted on the sliding member 312, and the driving assembly 32 is drivingly connected to the sliding member 312. The guide rail assembly 31 is in linear sliding fit with the guide rail 311 through the sliding part 312, so as to realize linear driving of the magnetic part 11, the guide rail 311 serves as a guiding basis, and when the driving part 32 is used for driving the sliding part 312 to linearly move along the track of the guide rail 311, the magnetic part 11 can be driven to linearly move (i.e. to move along the radial direction of the disc chip 1).

The driving component 32 may adopt a gear structure, a connecting rod 326 structure, etc., and the driving precision of the guide rail component 31 may be improved by indirect driving, so as to improve the precision of the movement of the magnetic particles and ensure that the detection result has higher precision.

As shown in fig. 1 and 2, in one embodiment, the driving assembly 32 includes a second motor 321, a first gear set 322, and at least one rack 323; the driving end of the second motor 321 is in driving connection with the driving end of the first gear set 322, and the driven end of the first gear set 322 is in driving connection with the rack 323; the rack 323 is connected to the slider 312.

The first gear set 322 has the advantage of high driving precision through the meshing transmission of a plurality of gears, and can ensure high-precision linear movement of the sliding member 312 on the guide rail 311. When the driving assembly 32 works, the second motor 321 is started, and the driving end of the first gear set 322 is driven to rotate by the driving end of the second motor 321 (the driving end is also the first gear in the first gear set 322, which is in driving connection with the second motor 321), so that the driven end of the first gear set 322 is engaged with the rack 323 for transmission (i.e. the last gear in the first gear set 322 and the gear engaged with the rack 323) by the transmission cooperation of the plurality of gears in the first gear set 322, and the sliding member 312 is driven to move linearly on the guide rail 311.

The second motor 321 may be a stepping motor, a servo motor, or the like. The first gear set 322 located between the second motor 321 and the rack 323 can adjust the transmission ratio between the gears according to the requirement, so as to cooperate with the rotation speed, the step angle, etc. of the second motor 321, and realize the precise control of the linear movement distance of the sliding member 312.

Preferably, the first gear set 322 includes a first driving gear 3221, a first intermediate gear 3222, a second intermediate gear 3223 and at least one first driven gear 3224; the first intermediate gear 3222 and the second intermediate gear 3223 are coaxially and synchronously assembled in a rotating manner, and the highest point of the first intermediate gear 3222 is lower than the lowest point of the first driven gear 3224; the driving end of the second motor 321 is drivingly connected to the first intermediate gear 3222 through the first driving gear 3221, and the second intermediate gear 3223 is drivingly connected to the rack 323 through the first driven gear 3224.

In the first gear set 322 formed by the first driving gear 3221, the first intermediate gear 3222, the second intermediate gear 3223 and the first driven gear 3224, the first driving gear 3221 belongs to a driving end of the first gear set 322, the second motor 321 is drivingly connected to the first driving gear 3221 to drive the first driving gear 3221 to rotate, and the first driving gear 3221 is engaged with the first intermediate gear 3222 to drive the first intermediate gear 3222 to rotate. The second intermediate gear 3223 and the first intermediate gear 3222 rotate coaxially and synchronously, so when the first intermediate gear 3222 rotates, the second intermediate gear 3223 is driven to rotate, and the second intermediate gear 3223 is meshed with the first driven gear 3224 to drive the first driven gear 3224 to rotate. A first driven gear 3224 belongs to the driven end of the first gear set 322, and the first driven gear 3224 is meshed with the rack 323 to drive the rack 323 to move, and indirectly drives the sliding member 312 to make a linear movement on the guide rail 311.

The first intermediate gear 3222 and the second intermediate gear 3223 may be coaxially connected by a common shaft, or the first intermediate gear 3222 and the second intermediate gear 3223 may be coaxially and integrally formed (i.e., "i" shaped in cross section), so long as the first intermediate gear 3222 and the second intermediate gear 3223 are coaxially and synchronously rotated. The first gear set 322 may be formed by other number of gears besides four gears, i.e., the first driving gear 3221, the first intermediate gear 3222, the second intermediate gear 3223 and the first driven gear 3224, and is not limited herein.

It should be noted that, since the second intermediate gear 3223 indirectly drives the relative movement between the slider 312 and the guide track 311 through the first driven gear 3224 and the rack 323, the second intermediate gear 3223, the first driven gear 3224 and the rack 323 are approximately in the same plane, in order to prevent the second motor 321 from interfering with the guide track assembly 31 in the circumferential direction of the second intermediate gear 3223 when the guide track assembly 31 is driven by the first motor 321 indirectly through the rack 323 through the first gear set 322, at least the first intermediate gear 3222 is ensured not to interfere with the first driven gear 3224, so that the highest point of the first intermediate gear 3222 is lower than the lowest point of the first driven gear 3224.

Especially, when the reaction chambers 12 are provided in plural numbers, the plural reaction chambers 12 are distributed in the circumferential direction of the disk chip 1, and the corresponding guide rail assemblies 31 are also required to be correspondingly provided in plural numbers along the circumferential direction of the disk chip 1. In order to synchronously drive the plurality of track assemblies 31, the plurality of first driven gears 3224 are in mesh transmission with the second intermediate gears 3223 at different positions along the circumference of the second intermediate gears 3223. If it is required to ensure that the second motor 321 does not interfere with the plurality of first driven gears 3224 circumferentially distributed on the second intermediate gear 3223 at the same time when driving the first intermediate gear 3222, at least the highest point of the first intermediate gear 3222 is required to be lower than the lowest point of the first driven gear 3224. The relative distance between the two can be set according to practical situations, and is not limited herein. The advantage of such an arrangement is that an effective transmission between the drive assembly 32 and the guide rail assembly 31 can be ensured, and the arrangement of a plurality of reaction chambers 12 is also suitable, so that the assembly of the whole structure is reasonable and effective.

As shown in fig. 3 and 4, in another embodiment, the drive assembly 32 includes a third motor 324, a second gear set 325, and at least one link 326; the driving end of the third motor 324 is in driving connection with the driving end of the second gear set 325, and the driven end of the second gear set 325 is in hinged driving with the sliding member 312 through the connecting rod 326.

The second gear set 325 has the advantage of high driving precision through the meshing transmission of a plurality of gears, and the slider 312 is driven by the third motor 324 to linearly move on the guide rail 311 through the connecting rod 326, so that the slider 312 can be ensured to linearly move on the guide rail 311 with high precision. When the driving assembly 32 works, the third motor 324 is started, and the driving end of the second gear set 325 is driven to rotate (the driving end is also the first gear in the second gear set 325, which is in driving connection with the third motor 324) by the driving end of the third motor 324, so that the driven end of the second gear set 325 is driven to be connected with the connecting rod 326 (i.e. the last gear in the second gear set 325 and the gear connected with the connecting rod 326) by the transmission cooperation of the plurality of gears in the second gear set 325, and the connecting rod 326 drives the sliding member 312 to move linearly on the guide rail 311.

The third motor 324 may be a stepping motor, a servo motor, or the like. The second gear set 325 between the third motor 324 and the connecting rod 326 can adjust the transmission ratio between the gears according to the requirement, so as to cooperate with the rotating speed, the step angle, etc. of the third motor 324 to realize the precise control of the linear moving distance of the sliding member 312.

Preferably, the second gear set 325 includes a second driving gear 3251, a third intermediate gear 3252 and a fourth intermediate gear 3253; the third intermediate gear 3252 and the fourth intermediate gear 3253 are coaxially and synchronously assembled in a rotating manner, and the highest point of the third intermediate gear 3252 is lower than the lowest point of the connecting rod 326; the driving end of the third motor 324 is drivingly connected to the third intermediate gear 3252 through the second driving gear 3251, and the fourth intermediate gear 3253 is hingedly driven to the slide 312 through the connecting rod 326.

In the second gear set 325 formed by the second driving gear 3251, the third intermediate gear 3252 and the fourth intermediate gear 3253, the second driving gear 3251 belongs to the driving end of the second gear set 325, the third motor 324 is drivingly connected to the second driving gear 3251 to drive the second driving gear 3251 to rotate, and the second driving gear 3251 is meshed with the third intermediate gear 3252 to drive the third intermediate gear 3252 to rotate. Fourth intermediate gear 3253 and third intermediate gear 3252 rotate coaxially and synchronously, so that when third intermediate gear 3252 rotates, fourth intermediate gear 3253 is driven to rotate, and fourth intermediate gear 3253 belongs to the driven end of first gear set 322. The fourth intermediate gear 3253 is in hinged connection with the slider 312 via the connecting rod 326, and indirectly drives the slider 312 to move linearly on the guide rail 311.

The third intermediate gear 3252 and the fourth intermediate gear 3253 may be coaxially connected by a common shaft, or the third intermediate gear 3252 and the fourth intermediate gear 3253 may be provided as a structure formed by coaxially and integrally molding (the cross section of the structure is in an "i" shape), as long as the third intermediate gear 3252 and the fourth intermediate gear 3253 are coaxially and synchronously rotated. The second gear set 325 may be formed by three gears, i.e., a second driving gear 3251, a third intermediate gear 3252, and a fourth intermediate gear 3253, and may be formed by another number of gears, which is not limited herein.

It should be noted that, since the fourth intermediate gear 3253 indirectly drives the relative movement between the sliding member 312 and the guide rail 311 through the hinged connecting rod 326, the fourth intermediate gear 3253 and the connecting rod 326 may be substantially in the same plane, for example, the connecting rod 326 is hinged on the upper surface or the lower surface of the fourth intermediate gear 3253. In order to prevent the third motor 324 from interfering with the track assembly 31 in the circumferential direction of the fourth intermediate gear 3253 when the track assembly 31 is driven by the second gear set 325 indirectly through the connecting rod 326, at least the third intermediate gear 3252 is ensured not to interfere with the connecting rod 326, so that the highest point of the third intermediate gear 3252 is lower than the lowest point of the connecting rod 326.

Especially, when the reaction chambers 12 are provided in plural numbers, the plural reaction chambers 12 are distributed in the circumferential direction of the disk chip 1, and the corresponding guide rail assemblies 31 are also required to be correspondingly provided in plural numbers along the circumferential direction of the disk chip 1. To drive the plurality of track assemblies 31 synchronously, a plurality of connecting rods 326 are hinged to fourth intermediate gear 3253 at different positions along the circumference of fourth intermediate gear 3253. If it is necessary to ensure that the third motor 324 does not interfere with the plurality of connecting rods 326 distributed around the circumference of the fourth intermediate gear 3253 when driving the third intermediate gear 3252, at least the highest point of the third intermediate gear 3252 needs to be lower than the lowest point of the connecting rods 326. The relative distance between the two can be set according to practical situations, and is not limited herein. The advantage of such an arrangement is that an effective transmission between the drive assembly 32 and the guide rail assembly 31 can be ensured, and the arrangement of a plurality of reaction chambers 12 is also suitable, so that the assembly of the whole structure is reasonable and effective.

As shown in fig. 5 and 6, in another embodiment, the driving assembly 32 includes a fourth motor 327, a third driving gear 328, a second driven gear 329 having at least one guide hole 3291, and a guide 3292 engaged with the guide hole 3291; the driving end of the fourth motor 327 is in driving connection with the third driving gear 328, and the third driving gear 328 is engaged with the second driven gear 329; the second driven gear 329 is coaxially arranged with the disk chip 1, the guide hole 3291 is arc-shaped, and the distance from one end of the guide hole 3291 to the other end of the guide hole 3291 to the circle center of the second driven gear 329 is gradually reduced; the guide 3292 is connected with the slider 312 and slidably engaged along the track of the guide hole 3291, and the slider 312 reciprocates along the guide rail 311 under the driving of the guide 3292; the slider 312 is provided with a support 3293, and the magnetic member 11 is mounted on the support 3293 and located above the second driven gear 329.

The first gear set 322 has the advantage of high driving precision through multiple gear mesh transmission, and meanwhile, when the sliding piece 312 is driven to linearly move on the guide rail 311 through the second driven gear 329, the sliding piece 312 can be driven to linearly move on the guide rail 311 through small displacement when the second driven gear 329 is rotated at a large angle by virtue of the matching between the arc-shaped guide hole 3291 arranged on the second driven gear 329 and the guide piece 3292 connected with the sliding piece 312, so that the sliding piece 312 can be ensured to linearly move on the guide rail 311 at high precision.

When the driving assembly 32 works, the fourth motor 327 is started, the driving end of the fourth motor 327 drives the third driving gear 328, the third driving gear 328 drives the second driven gear 329 to rotate, and indirectly drives the sliding member 312 to move linearly on the guide rail 311 through the guiding cooperation of the guiding hole 3291 and the guiding member 3292. Since the guide hole 3291 is arc-shaped, and the distance from one end of the guide hole 3291 to the center of the second driven gear 329 is gradually reduced, when the second driven gear 329 is driven to rotate by the third driving gear 328, the guide hole 3291 and the guide piece 3292 are in guide fit, so that the guide piece 3292 can move along the track of the guide hole 3291, and during the movement of the guide piece 3292, the sliding piece 312 is driven to linearly move along the radial direction of the second driven gear 329 according to the change in the distance between the guide hole 3291 and the center of the second driven gear 329. Meanwhile, the second driven gear 329 is coaxially disposed with the disk chip 1, so that the moving accuracy of the slider 312 can be greatly improved by converting a large distance movement of the guide 3292 in the guide hole 3291 into a small distance movement of the slider 312 on the guide rail 311, thereby improving the moving accuracy of the magnetic member 11 and the magnetic particles.

The fourth motor 327 may be a stepping motor or a servo motor. The arc degree of guiding hole 3291 can be set as required to cooperate with the rotational speed, step angle, etc. of fourth motor 327, so as to realize the accurate control of the linear movement distance of sliding member 312.

When the reaction chambers 12 are provided in plural numbers, the plural reaction chambers 12 are distributed in the circumferential direction of the disk chip 1, and the corresponding guide rail assemblies 31 are also required to be correspondingly provided in plural numbers along the circumferential direction of the disk chip 1. In order to synchronously drive the plurality of rail assemblies 31, a plurality of guide holes 3291 of the second driven gear 329 are correspondingly provided.

As shown in fig. 7, preferably, the guide 3292 includes a roller shaft and a roller 3294 fitted on the roller shaft; the roller shaft is mounted on the slider 312 in the axial direction of the second driven gear 329, and the roller 3294 is in rolling engagement with the guide hole 3291. By adopting the structure that the roller 3294 is matched with the roller shaft, the guide hole 3291 and the roller 3294 can move in a rolling friction mode when moving in a relative matching way, so that the smoothness of the movement between the two is ensured, and the part abrasion caused by friction is reduced.

Preferably, the support 3293 passes through the guide hole 3291. When the support 3293 passes through the guide hole 3291, the support 3293 and the guide 3292 move synchronously in the guide hole 3291 after the second driven gear 329 rotates, and the support 3293 ensures the radial movement of the magnetic element 11, which is simple and reliable.

The utility model also provides a detection device, include disc chip magnetic particle mobile device. Since the detailed structure, functional principle and technical effect of the mobile device are described in detail in the foregoing, the related contents are not repeated herein. For any technical content related to the mobile device, reference is made to the above description.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention.

Claims

1. A disk chip magnetic particle moving apparatus, comprising:

a disk chip having at least one reaction chamber;

2. A disk chip magnetic particle shifting apparatus according to claim 1, wherein the centrifugal control mechanism includes a first motor, a first rotating shaft, and a chip mounting structure;

3. The disc chip magnetic particle moving device according to claim 2, wherein the chip mounting structure comprises a mounting disc and a fixed shaft disposed along a central axis of the mounting disc, the mounting disc is in transmission connection with the first rotating shaft and the central axes of the mounting disc and the first rotating shaft are coincident, and a central shaft hole of the disc chip is in sleeve fit with the fixed shaft;

the disk chip is positioned opposite to the mounting disk.

4. The apparatus as claimed in claim 3, wherein the mounting plate is provided with at least one positioning post, and the disc chip is provided with a positioning hole corresponding to the positioning post;

5. A disk chip magnetic particle moving apparatus according to claim 3, wherein the side wall of the fixed shaft is provided with at least one positioning protrusion, and the inner hole wall of the central shaft hole is provided with a positioning groove corresponding to the positioning protrusion;

6. The disc chip magnetic particle moving device according to claim 1, wherein the reaction chamber includes a first reaction chamber, a second reaction chamber and a third reaction chamber arranged along a circumferential direction of the disc chip;

7. A disk chip magnetic particle transporting device according to any one of claims 1 to 6, wherein said rail assembly includes a rail and a slider; the sliding part is assembled with the guide rail in a linear sliding mode, the magnetic part is installed on the sliding part, and the driving assembly is connected with the sliding part in a driving mode.

8. The disc chip magnetic particle moving apparatus according to claim 7, wherein the driving assembly includes a second motor, a first gear set, and at least one rack; the driving end of the second motor is in driving connection with the driving end of the first gear set, and the driven end of the first gear set is in driving connection with the rack;

the rack is connected with the sliding piece.

9. A disc chip magnetic particle shifting apparatus according to claim 8, wherein the first gear set includes a first driving gear, a first intermediate gear, a second intermediate gear and at least one first driven gear; the first intermediate gear and the second intermediate gear are coaxially and synchronously assembled in a rotating mode, and the highest point of the first intermediate gear is lower than the lowest point of the first driven gear;

10. A disc chip magnetic particle moving apparatus according to claim 7, wherein said driving assembly includes a third motor, a second gear set and at least one link; the driving end of the third motor is in driving connection with the driving end of the second gear set, and the driven end of the second gear set is hinged with the sliding piece through the connecting rod for driving.

11. A disc chip magnetic particle shifting apparatus according to claim 10, wherein the second gear set includes a second driving gear, a third intermediate gear and a fourth intermediate gear; the third intermediate gear and the fourth intermediate gear are coaxially and synchronously assembled in a rotating mode, and the highest point of the third intermediate gear is lower than the lowest point of the connecting rod;

12. A disc chip magnetic particle moving apparatus according to claim 7, wherein said driving assembly includes a fourth motor, a third driving gear, a second driven gear having at least one guide hole, and a guide member engaged with said guide hole;

13. A disk chip magnetic particle transporting apparatus according to claim 12, wherein said guide member includes a roller shaft and a roller fitted on said roller shaft; the roller shaft is mounted on the sliding piece along the axial direction of the second driven gear, and the roller is in rolling fit with the guide hole.

14. A disk chip magnetic particle moving apparatus according to claim 12, wherein said support member passes through said guide hole.

15. A detecting device comprising the disk chip magnetic particle moving device according to any one of claims 1 to 14.