CN210665606U - Low-temperature electrophoresis film-rotating ice box with observation window - Google Patents

Low-temperature electrophoresis film-rotating ice box with observation window Download PDF

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Publication number
CN210665606U
CN210665606U CN201921532453.3U CN201921532453U CN210665606U CN 210665606 U CN210665606 U CN 210665606U CN 201921532453 U CN201921532453 U CN 201921532453U CN 210665606 U CN210665606 U CN 210665606U
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box body
observation window
square
square box
ice box
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CN201921532453.3U
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Chinese (zh)
Inventor
胡兰婷
陈功星
谢恬
方晓明
阎辉
赵起超
朱茵
朱静怡
丁钟灵
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Hangzhou Normal University
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Hangzhou Normal University
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Abstract

The utility model discloses a take low temperature electrophoresis of observation window to change membrane ice box, including square box body, be sandwich structure between the inside and outside wall of square box body, it has the refrigerant to pour into in the sandwich, and the inside of square box body is provided with berle electrophoresis tank, is provided with the observation window on the lateral wall of square box body broadside, and the bottom of square box body is provided with the support limit of downward salient. The utility model discloses the ice box, the cooling function of existing ordinary ice box avoids the high temperature, can pass through observation window again, observes electrophoresis bromophenol blue, marker's position, and the convenience changes the judgement of membrane process to the electrophoresis, saves time. Meanwhile, the transparent observation window has a certain heat insulation effect, so that the action time of the ice box is conveniently prolonged, and meanwhile, the arrangement of the supporting edge can effectively avoid unstable placement caused by the fact that the bottom of the ice box is protruded due to the freezing of the freezing agent.

Description

Low-temperature electrophoresis film-rotating ice box with observation window
Technical Field
The utility model relates to an electrophoresis changes membrane ice box, concretely relates to low temperature electrophoresis of taking observation window changes membrane ice box.
Background
Electrophoresis refers to the phenomenon in which charged particles or molecules move in an electric field toward an electrode of opposite polarity to the electric field. A technique of achieving separation by utilizing a difference in moving speed of charged particles in an electric field is called an electrophoresis technique. The electrophoresis technology is mainly used for research of proteins, nucleic acids, enzymes, even viruses and cells besides being used for separation and analysis of small molecular substances. Polyacrylamide gel electrophoresis can be used for protein purity identification, SDS polyacrylamide gel electrophoresis can be used for protein molecular weight determination, polyacrylamide gel electrophoresis can be used for protein quantification, agar or agarose gel immunoelectrophoresis can be used for checking purity analysis of protein preparations, component research of protein mixtures, whether antiserum preparations have antibodies against a certain known antigen, and whether two antigens are the same. The electrophoresis method has the characteristics of simple equipment, convenient operation, high resolution and selectivity, and becomes a commonly used technology in medical inspection technology.
Whether electrophoresis is successful or not is one of the important factors for the experiment to be smoothly performed. In the electrophoresis process, voltage can generate heat, biological samples such as protein and the like are not heat-resistant, and if the temperature is too high, the strip can deform, so that the experimental result is influenced. Meanwhile, in the process of film transfer, a large amount of heat is generated when the wet transfer current is large, the gel expands after absorbing heat, and a gap is possibly generated between the film and the gel, so that the film transfer is not uniform. At present, the most common cooling method is to use crushed ice and a foam box, and in the experimental process, the inventors find that the crushed ice has no observation window during electrophoresis, and cannot observe the positions of bromophenol blue and marker, thereby influencing the judgment of the electrophoresis process. And the foam box is used repeatedly, can lead to its bottom deformation to be outstanding because the ice-cube solidifies, and it is unstable to place, influences the use.
SUMMERY OF THE UTILITY MODEL
To the problem that exists among the prior art, the utility model aims to provide a take low temperature electrophoresis of observation window to change membrane ice box.
The utility model discloses a following technical scheme realizes:
a take low temperature electrophoresis of observation window to change membrane ice box, its characterized in that includes square box body, be sandwich structure between the inside and outside wall of square box body, the injection has the refrigerant in the sandwich, the inside of square box body is provided with berle electrophoresis tank, be provided with the observation window on the lateral wall of square box body broadside, the bottom of square box body is provided with the support limit of downward salient.
The low-temperature electrophoresis film-rotating ice box with the observation window is characterized in that the length of the square box body is 30 +/-1 cm, the width of the square box body is 26 +/-1 cm, and the height of the square box body is 27 +/-1 cm.
The low-temperature electrophoresis film-transferring ice box with the observation window is characterized in that the height of the refrigerant in the interlayer is 4/5 of the total height of the interlayer.
The low-temperature electrophoresis film-rotating ice box with the observation window is characterized in that the thickness of the inner wall and the outer wall of the square box body is 3 +/-1 cm.
The low-temperature electrophoresis film-rotating ice box with the observation window is characterized in that the observation window is a square notch integrally formed in the side wall of the square box body, recessed grooves are formed in the two side faces of the square notch, and an organic glass plate matched with the space of the square notch is inserted into the square notch.
The low-temperature electrophoresis film-rotating ice box with the observation window is characterized in that protruding edges movably connected with grooves in an inserted mode are integrally arranged on two sides of an organic glass plate, and the outer walls of the protruding edges are matched with the inner walls of the grooves.
The low-temperature electrophoresis film-rotating ice box with the observation window is characterized in that the length and the width of the square notch are 14cm, and the square notch is positioned in the middle of the side wall of the square box body.
The low-temperature electrophoresis film-rotating ice box with the observation window is characterized in that the support edge is arranged around the lower bottom of the square box body, and the height of the support edge is 2 +/-0.5 cm.
The utility model discloses the ice box, the cooling function of existing ordinary ice box avoids the high temperature, can pass through observation window again, observes electrophoresis bromophenol blue, marker's position, and the convenience changes the judgement of membrane process to the electrophoresis, saves time. Meanwhile, the transparent observation window has a certain heat insulation effect, so that the action time of the ice box is conveniently prolonged, and meanwhile, the arrangement of the supporting edge can effectively avoid unstable placement caused by the fact that the bottom of the ice box is protruded due to the freezing of the freezing agent.
Drawings
FIG. 1 is a view of the overall structure of the present invention;
FIG. 2 is a schematic structural view with the glass plate removed;
FIG. 3 is a schematic view of a plastic glazing panel structure;
in the figure, 1-square box body, 2-observation window, 3-supporting edge, 4-square gap, 5-glass plate, 6-groove and 7-rib.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings, and specific embodiments will be given.
As shown in fig. 1-3, the utility model relates to a take low temperature electrophoresis of observation window to change membrane ice box, this ice box include that low temperature electrophoresis changes membrane ice box and organic glass board two parts, and concrete structure is the square box body including the synthetic resin material, and this box body size is: the length is 30 plus or minus 1cm, the width is 26 plus or minus 1cm, the height is 27 plus or minus 1cm, the thickness of the inner wall and the outer wall of the square box body is 3 plus or minus 1cm, a sandwich structure is arranged between the inner wall and the outer wall of the square box body, a refrigerant is injected into the sandwich structure, and the height of the refrigerant in the sandwich layer is 4/5 of the total height of the sandwich layer.
The inside of square box body can place the berle electrophoresis tank, electrophoresis tank periphery is 3-4cm apart from box body inner wall interval, be provided with observation window on the lateral wall of square box body broadside, this observation window is the square breach that the integral type set up on the lateral wall of square box body, the length and width of square breach is 14cm, and square breach is located the middle part position of square box body lateral wall, this square breach both sides face is provided with the recess that caves in, the grafting sets up rather than the identical organic glass board in space, this organic glass board adopts polymethyl methacrylate, the integral type is provided with the bead identical with the recess on the both sides face of glass board, this setting makes the glass board with can directly peg graft, also can freely separate with square box body.
For the deformation that prevents in the ice chest use, improve the stability of using, be provided with the support limit of downward salient in the bottom of square box body, should support limit and square box body integrated into one piece setting, and support and set up all around along bottom under the square box body, its height is 2 0.5cm, prevents to lead to ice chest bottom deformation and place unstability because the volume expansion after the refrigerant freezes.

Claims (8)

1. The utility model provides a take low temperature electrophoresis of observation window to change membrane ice box, its characterized in that includes square box body (1), be sandwich structure between the interior outer wall of square box body (1), it has the refrigerant to pour into in the sandwich, berle electrophoresis tank is placed to the inside of square box body (01), be provided with observation window (2) on the lateral wall of square box body (1) broadside, the bottom of square box body (1) is provided with support limit (3) of downward salient.
2. The cryoelectrophoresis film-rotating ice box with an observation window as claimed in claim 1, wherein the square box body (1) has a length of 30 ± 1cm, a width of 26 ± 1cm and a height of 27 ± 1 cm.
3. The cryoelectrophoresis film-transferring ice box with a viewing window of claim 1, wherein the height of said coolant in said compartment is 4/5 of the total height of said compartment.
4. The cryoelectrophoresis film-rotating ice box with an observation window as claimed in claim 1, wherein the thickness of the inner and outer walls of the square box body (1) is 3 ± 1 cm.
5. The low-temperature electrophoresis film-rotating ice box with the observation window according to claim 1, wherein the observation window (2) is a square notch (4) integrally formed on the side wall of the square box body (1), two side faces of the square notch (4) are provided with recessed grooves (6), and an organic glass plate (5) matched with the square notch in space is inserted and arranged on the square notch.
6. The low-temperature electrophoresis film-rotating ice box with the observation window according to claim 5, wherein the two sides of the organic glass plate (5) are integrally provided with a rib (7) movably inserted into the groove (6), and the outer wall of the rib (7) is matched with the inner wall of the groove (6).
7. The low-temperature electrophoresis film-rotating ice box with the observation window as claimed in claim 5, wherein the length and width of the square notch (4) are both 14cm, and the square notch (4) is located at the middle position of the side wall of the square box body (1).
8. The low-temperature electrophoresis film-rotating ice box with the observation window as claimed in claim 1, wherein the supporting edge (3) is arranged along the periphery of the lower bottom of the square box body (1), and the height of the supporting edge is 2 +/-0.5 cm.
CN201921532453.3U 2019-09-16 2019-09-16 Low-temperature electrophoresis film-rotating ice box with observation window Active CN210665606U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201921532453.3U CN210665606U (en) 2019-09-16 2019-09-16 Low-temperature electrophoresis film-rotating ice box with observation window

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201921532453.3U CN210665606U (en) 2019-09-16 2019-09-16 Low-temperature electrophoresis film-rotating ice box with observation window

Publications (1)

Publication Number Publication Date
CN210665606U true CN210665606U (en) 2020-06-02

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201921532453.3U Active CN210665606U (en) 2019-09-16 2019-09-16 Low-temperature electrophoresis film-rotating ice box with observation window

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CN (1) CN210665606U (en)

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