CN210641825U - Cultivation device for collecting submerged plant root secretion - Google Patents

Cultivation device for collecting submerged plant root secretion Download PDF

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Publication number
CN210641825U
CN210641825U CN201920591909.7U CN201920591909U CN210641825U CN 210641825 U CN210641825 U CN 210641825U CN 201920591909 U CN201920591909 U CN 201920591909U CN 210641825 U CN210641825 U CN 210641825U
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root system
container
nutrient solution
splendid attire
root
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Expired - Fee Related
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CN201920591909.7U
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Chinese (zh)
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钱昱
乔诗楠
方瑜鸿
杨新鹏
安昕
刘玉昊
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Yunnan University YNU
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Yunnan University YNU
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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Abstract

The utility model discloses a collect cultivation ware of submerged plant root system secretion, including advancing the flowing back pipe, exhaust duct, the container sealing lid, the rhizome fixture, the shading lagging, root system nutrient solution splendid attire container, the container sealing lid sets up on root system nutrient solution splendid attire container, the last root system through-hole that is equipped with of container sealing lid, submerged plant root system passes the root system through-hole, the rhizome fixture is filled and is set up with fixed submerged plant between submerged plant rhizome junction and the container sealing lid, advance the flowing back pipe and pass the sealed bottom that covers of container and extend to root system nutrient solution splendid attire container, exhaust duct passes the sealed inside that covers of container and extend to root system nutrient solution splendid attire container, root system nutrient solution is installed in root system nutrient solution splendid attire container, root system nutrient solution splendid attire container. The cultivation device can overcome the errors of the experimental growth environment and the real environment of the plants, and simultaneously, the qualitative and quantitative determination of the growth environment of the root system is carried out by separating the growth environment of the roots and the leaves.

Description

Cultivation device for collecting submerged plant root secretion
Technical Field
The utility model relates to a collect cultivation ware of submerged plant root system secretion belongs to aquatic plant cultivation ware technical field.
Background
Plants that can grow in water are collectively referred to as aquatic plants. Aquatic plants are excellent swimmers or divers. The leaves are soft and transparent, and some leaves are formed into filaments, such as Aureobasidium. The filamentous leaves can greatly increase the contact area with water, so that the leaves can obtain light which is rarely obtained in the water to the maximum extent, and absorb carbon dioxide which is rarely dissolved in the water, thereby ensuring the photosynthesis. The research on aquatic plants and their growers has never been stopped by those skilled in the art.
The submerged plant cultivation method can be used for adjusting the submerged height of the submerged plant and providing certain illumination for the submerged plant at proper time. The method is mainly used for submerged plant cultivation, and does not relate to an auxiliary cultivation method for submerged plant root secretion experiments.
Aquatic plant root system secretes oxygen experimental apparatus through establishing the anaerobic operation case, establishes the detector, simple structure, and the preparation cost is low, and convenient to carry is applicable to the root system and secretes oxygen in real time and detects. But is not suitable for long-term exposure experiments and researches on other substances such as plant root secretion and the like, and is only limited to monitoring of plant root secretion oxygen experiments.
Collect the device of plant root system secretion, can collect the root system secretion that all roots produced in the plant growth development process to through twice filtration, the collection that can be quantitative and the root system secretion content of survey plant, with the total amount and the change law of survey plant growth in-process root system secretion, with the research of perfect plant allelopathy. However, the experiment is novel and mainly aims at researching the allelopathy effect caused by the root exudates of the soil-cultured plants, and the experimental auxiliary cultivation mode of the root exudates of the submerged plants is not involved.
The wetland plant root secretion continuous collection device and the wetland plant root secretion continuous collection method for the experiment have the advantages that the wetland plant root secretion continuous collection device and the wetland plant root secretion continuous collection method are used for adjusting devices of different environmental factors of plants, different cultivation conditions can be designed in a split-box splicing mode for carrying out the experiment, the whole experiment adjustable range is large, and the measured experiment result is more valuable. However, the experiment model mainly simulates root secretions of aquatic plants in soil, and partial substances in the soil can also influence the secretions. And the novel simulation root system interstitial water of this experiment can separate the root system secretion that is not influenced by the environment.
The root system of the submerged plant is rooted in the bottom mud, and the root, the stem and the leaf all grow in the water body, so that the submerged plant has the function of absorbing water body pollutants. The ability of the root system to modify the sediment environment is most significant because the aquatic plant root system has the ability to absorb nutrients and contaminants in the interstitial water of the sediment on the one hand, and can also change the nature of the sediment through the way of transporting oxygen to the root system and secreting root exudates on the other hand. However, the composition of the substances in the sediment is complex, which is not beneficial to the research on the nature and concentration of the components of the root exudates. In order to thoroughly research the root system oxygen secretion and root system secretion of submerged plant in different growth environments, it is necessary to eliminate the interference of sediments and design a simulation system based on the submerged plant root system growth environment, so as to further research the material secretion characteristics of the plant root system in different root system growth environments.
Disclosure of Invention
An object of the utility model is to provide a collect cultivation ware of submerged plant root system secretion, through the root system environment of simulation submerged plant and the composition and the volume of solution in the accurate control root system growing environment, reach the purpose of studying root system secretion composition and concentration in the different growing environments of plant.
The utility model discloses an aquatic plant cultivation device is the cultivation ware of the supplementary measurement submerged plant root system oxygen secretion experiment, and the aquatic plant root system is in the cultivation ware of different cultivation environments promptly.
The utility model provides a collect cultivation ware of submerged plant root secretion, including advancing flowing back pipe 1, exhaust duct 2, the sealed lid of container 3, rhizome fixture 4, shading lagging 5, root system nutrient solution splendid attire container 6, the sealed lid of container 3 sets up on root system nutrient solution splendid attire container 6, be equipped with the root system through-hole on the sealed lid of container 3, the submerged plant root system passes the root system through-hole, rhizome fixture 4 is filled and is set up with fixed submerged plant between submerged plant rhizome junction and the sealed lid of container 3, advance flowing back pipe 1 and pass sealed lid of container 3 and extend to the bottom of root system nutrient solution splendid attire container 6, exhaust duct 2 passes sealed lid of container 3 and extends to the inside of root system nutrient solution splendid attire container 6, root system nutrient solution 7 is installed in root system nutrient solution splendid attire container 6, 6 outer wall cover of root system nutrient solution.
The distance between the bottom end of the exhaust conduit 2 and the bottom surface of the container sealing cover 3 is not more than 2 mm.
The rhizome fixing 4 is medical photosensitive resin or light-cured resin for dental profession.
The liquid inlet and outlet guide pipe 1, the gas outlet guide pipe 2, the container sealing cover 3 and the root system nutrient solution containing container 6 are all made of transparent glass materials or transparent plastic materials.
Still including splendid attire container, root system nutrient solution splendid attire container 6 evenly sets up in the splendid attire container.
The root system nutrient solution containing container 6 can adopt a centrifuge tube with a proper size or a plastic container, and the culture container can be adjusted according to the experiment requirement; the container sealing cover 3 can be adjusted by punching holes with an electric iron;
when the rhizome fixing object 4 is medical photosensitive resin, fixing the photosensitive resin connecting the plant and the container sealing cover by adopting a medical photocuring machine, so that the plant is fixed on the container sealing cover, and the purpose of separating root system and stem leaf environment is achieved;
the medical photosensitive resin can be replaced by a biological filling and fixing material which is nontoxic and harmless to organisms, and is preferably a photocuring resin for the department specialty;
the root system nutrient solution can be adjusted according to the experiment requirement;
the liquid inlet and outlet guide pipe 1 extends to the bottom of the root system nutrient solution container 6, and the distance from the bottom end of the gas outlet guide pipe 2 to the bottom surface of the container sealing cover 3 is not more than 2mm, so that the root system nutrient solution can be conveniently replaced and the pressure balance of the inner environment and the outer environment can be maintained; pour root system nutrient solution into root system nutrient solution splendid attire container, cover 3 with the sealed lid of container, get rid of unnecessary gas in the root system nutrient solution splendid attire container through exhaust duct 3 when necessary.
The root system of the submerged plant penetrates through the root system through hole, and the root and stem fixture 4 is filled between the root and stem connection part of the submerged plant and the container sealing cover 3 to fix the submerged plant; the rhizome connection is in a cover surface level, the circulation of different upper and lower nutrition environments through a gap space at the rhizome connection part is prevented, and the rhizome environment of the plant is separated;
the utility model has the advantages that:
(1) the utility model discloses the cultivation ware of collecting submerged plant root system secretion can create the true environment of root system clearance water under the experimental condition to modify the clearance water composition based on the demand of experiment, reach the purpose of survey submerged plant root system secretion composition and concentration under the different clearance water environment; the submerged plant root system is directly exposed in an artificially simulated water environment, and the components of the water environment are adjusted according to the experimental purpose, so that the purpose of simulating the interstitial water in different sediments is achieved, and the research on the components and the concentration of plant root secretion growing in different sediments is facilitated;
(2) the device can be used for researching nutrient supply of submerged plant root systems in different growth media, plant root system secretion and the like;
(3) the utility model utilizes a root system environment simulation method to fix the junction of the plant root system and the stem part on the cover, separates the plant root and the stem, and overcomes the culture environment deviation caused by different submerged root and stem environments in the experimental culture process and the same root and stem environments in the actual culture process; stripping the root growth environment and determining the components and the total volume of the root growth environment, which is favorable for researching the absorption condition of plant root nutrients on one hand and detecting the type and the content of root exudates on the other hand;
(4) the utility model discloses a cultivation ware of collection submerged plant root system secretion has overcome the experimental growing environment of plant and has differed great shortcoming that probably causes the error with real environment, through the growing environment of separation root, leaf simultaneously, carries out accurate nature and ration to root system growing environment, and it is simple to have a design, and the investment is less, repeatedly usable, advantages such as application scope is wide.
Drawings
FIG. 1 is a schematic structural view of a cultivation device for collecting root exudates of submerged plants;
FIG. 2 is a front view of a planter for collecting submerged plant root exudates;
FIG. 3 is a top view of the assembly of the liquid inlet and outlet conduits and the sealing cover of the container;
FIG. 4 is a schematic view of the container placed in a holding container;
in the figure, 1-liquid inlet and outlet guide pipe, 2-gas outlet guide pipe, 3-container sealing cover, 4-rhizome fixing part, 5-shading sleeve plate, 6-container for containing root system nutrient solution and 7-root system nutrient solution.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and specific embodiments. However, the present invention is not limited to the above-described embodiments, and various changes can be made without departing from the gist of the present invention within the scope of knowledge possessed by a person skilled in the art.
Example 1
An aquatic plant rhizome environment separation cultivation device, as shown in figures 1-3, comprises a liquid inlet and outlet conduit 1, an air outlet conduit 2, a container sealing cover 3, rhizome fixture 4, shading lagging 5, root system nutrient solution splendid attire container 6, the sealed lid of container 3 sets up on root system nutrient solution splendid attire container 6, be equipped with the root system through-hole on the sealed lid of container 3, the submerged plant root system passes the root system through-hole, rhizome fixture 4 is filled and is set up with fixed submerged plant between submerged plant rhizome junction and the sealed lid of container 3, advance flowing back pipe 1 and pass sealed lid of container 3 and extend to the bottom of root system nutrient solution splendid attire container 6, exhaust duct 2 passes sealed lid of container 3 and extends to the inside of root system nutrient solution splendid attire container 6, root system nutrient solution 7 is installed in root system nutrient solution splendid attire container 6, 6 outer wall covers of root system nutrient solution splendid attire container.
Example 2
The utility model provides a collect cultivation ware of submerged plant root secretion, including into flowing back pipe 1, exhaust duct 2, container sealing lid 3, rhizome fixture 4, shading lagging 5, root system nutrient solution splendid attire container 6, container sealing lid 3 sets up on root system nutrient solution splendid attire container 6, be equipped with the root system through-hole on the container sealing lid 3, the submerged plant root system passes the root system through-hole, rhizome fixture 4 is filled and is set up between submerged plant rhizome junction and container sealing lid 3 with fixed submerged plant, it passes container sealing lid 3 and extends to the bottom of root system nutrient solution splendid attire container 6 to advance flowing back pipe 1, exhaust duct 2 passes container sealing lid 3 and extends to the inside of root system nutrient solution splendid attire container 6, root system nutrient solution 7 is installed in root system nutrient solution splendid attire container 6, root system nutrient solution splendid attire container 6 outer wall cover is equipped with shading;
the distance between the bottom end of the exhaust guide pipe 2 and the bottom surface of the container sealing cover 3 is not more than 2 mm;
the rhizome fixing object 4 is medical photosensitive resin or light-cured resin for dental profession; the liquid inlet and outlet guide pipe 1, the gas outlet guide pipe 2, the container sealing cover 3 and the root system nutrient solution containing container 6 are all made of transparent glass materials or transparent plastic materials.
Example 3
The utility model provides a collect cultivation ware of submerged plant root secretion, including into flowing back pipe 1, exhaust duct 2, container sealing lid 3, rhizome fixture 4, shading lagging 5, root system nutrient solution splendid attire container 6, container sealing lid 3 sets up on root system nutrient solution splendid attire container 6, be equipped with the root system through-hole on the container sealing lid 3, the submerged plant root system passes the root system through-hole, rhizome fixture 4 is filled and is set up between submerged plant rhizome junction and container sealing lid 3 with fixed submerged plant, it passes container sealing lid 3 and extends to the bottom of root system nutrient solution splendid attire container 6 to advance flowing back pipe 1, exhaust duct 2 passes container sealing lid 3 and extends to the inside of root system nutrient solution splendid attire container 6, root system nutrient solution 7 is installed in root system nutrient solution splendid attire container 6, root system nutrient solution splendid attire container 6 outer wall cover is equipped with shading;
the distance between the bottom end of the exhaust guide pipe 2 and the bottom surface of the container sealing cover 3 is not more than 2 mm;
the rhizome fixing object 4 is medical photosensitive resin or light-cured resin for dental profession;
the liquid inlet and outlet guide pipe 1, the gas outlet guide pipe 2, the container sealing cover 3 and the root system nutrient solution container 6 are all made of transparent glass materials or transparent plastic materials;
the root system nutrient solution container is characterized by also comprising a container, wherein the root system nutrient solution container 6 is uniformly arranged in the container;
firstly, the root system of the submerged plant penetrates through a root system through hole of a container sealing cover, and a root and stem connecting part of the submerged plant is fixed on the container sealing cover by utilizing a root and stem fixture 4, so that the root and stem of the submerged plant are separated, and the root system and stem leaves are in different environments; then the root system culture solution is input through the liquid inlet and outlet guide pipe 1, so that the root system of the submerged plant survives in the simulated interstitial water; then the whole device is evenly placed in a container, such as a water jar, and submerged plant leaves similar living environment are injected into the container to form a whole culture system. Due to the absorption effect of plants, the culture solution in the root system environment needs to be replaced, the old culture solution is sucked out through the drainage guide pipe 1, the root secretion of the submerged plant is qualitatively and quantitatively analyzed, and then the new culture solution is pumped into the root system nutrient solution container 6 through the drainage guide pipe 1;
the root-stem connection part of the submerged plant is separated from the root-stem fixture 4 (photosensitive resin) through the container sealing cover to achieve the effect of separating the root-stem environment, and the liquid inlet and outlet guide pipe 1 on the container sealing cover can control the replacement of the root system nutrient solution in the root system nutrient solution container 6; root exudates generated by the single plant in different root growth environments can also be isolated.

Claims (5)

1. The utility model provides a collect cultivation ware of submerged plant root system secretion which characterized in that: comprises a liquid inlet and outlet conduit (1), an air outlet conduit (2), a container sealing cover (3), a rhizome fixing object (4), a shading sleeve plate (5) and a root system nutrient solution containing container (6), the sealed lid of container (3) sets up on root system nutrient solution splendid attire container (6), be equipped with the root system through-hole on the sealed lid of container (3), the submerged plant root system passes the root system through-hole, rhizome fixture (4) are filled and are set up with fixed submerged plant between the sealed lid of submerged plant rhizome junction and the sealed lid of container (3), advance flowing back pipe (1) and pass sealed lid of container (3) and extend to the bottom of root system nutrient solution splendid attire container (6), exhaust duct (2) pass sealed lid of container (3) and extend to the inside of root system nutrient solution splendid attire container (6), install in root system nutrient solution splendid attire container (6) root system nutrient solution (7), root system nutrient solution splendid attire container (6) outer wall cover is equipped with shading lagging plate (5.
2. The cultivator for collecting root exudates of submerged plants according to claim 1, wherein: the distance between the bottom end of the exhaust conduit (2) and the bottom surface of the container sealing cover (3) is not more than 2 mm.
3. The cultivator for collecting root exudates of submerged plants according to claim 1, wherein: the rhizome fixing object (4) is medical photosensitive resin.
4. The cultivator for collecting root exudates of submerged plants according to claim 1, wherein: the liquid inlet and outlet guide pipe (1), the air exhaust guide pipe (2), the container sealing cover (3) and the root system nutrient solution containing container (6) are all made of transparent glass or transparent plastic.
5. The cultivator for collecting root exudates of submerged plants according to claim 1, wherein: still include splendid attire container, root system nutrient solution splendid attire container (6) evenly sets up in the splendid attire container.
CN201920591909.7U 2019-04-28 2019-04-28 Cultivation device for collecting submerged plant root secretion Expired - Fee Related CN210641825U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201920591909.7U CN210641825U (en) 2019-04-28 2019-04-28 Cultivation device for collecting submerged plant root secretion

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201920591909.7U CN210641825U (en) 2019-04-28 2019-04-28 Cultivation device for collecting submerged plant root secretion

Publications (1)

Publication Number Publication Date
CN210641825U true CN210641825U (en) 2020-06-02

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Application Number Title Priority Date Filing Date
CN201920591909.7U Expired - Fee Related CN210641825U (en) 2019-04-28 2019-04-28 Cultivation device for collecting submerged plant root secretion

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