CN210596083U - Instrument-free on-site rapid detection card - Google Patents
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- CN210596083U CN210596083U CN201920758469.XU CN201920758469U CN210596083U CN 210596083 U CN210596083 U CN 210596083U CN 201920758469 U CN201920758469 U CN 201920758469U CN 210596083 U CN210596083 U CN 210596083U
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Abstract
The utility model discloses an instrument site short-term test card exempts from. The device comprises a sample inlet, a sample processing module, a reaction liquid flow path, a main reaction area and a result area which are sequentially arranged according to the flow direction of a sample, and is also provided with a puncture tube which is linked with the sample processing module and is internally provided with a cavity, and a reaction liquid cavity which is adjacent to or contacted with the puncture tube; the sample processing module moves and causes the puncture tube to displace, and further causes liquid in the reaction liquid cavity to flow out through the inner cavity of the puncture tube and flow through the sample processing module to enter the sample flow path. The detection card can realize the whole process from the original sample to the field detection of the result, is simple and convenient to operate, is quick, is not easy to make mistakes, only needs two-step operation of non-professionals, has low requirements on sample treatment, detection environment and personnel quality, has small pollution risk, extremely low false positive rate, does not depend on large or expensive instruments, has low cost, can be directly applied to field detection of complex and various samples, and has wide application range and good application prospect.
Description
Technical Field
The utility model belongs to the technical field of detect. And more particularly, to an instrument-free field rapid test card.
Background
The rapid detection card has the advantages of convenient and simple use, concise visualization mode, lower cost and the like, and has wide application prospect in various detection fields, such as nucleic acid detection cards. After decades of development and rapid maturation in recent years, nucleic acid detection technology is widely used in various fields such as detection of infectious disease pathogens (including bacteria, mycoplasma, viruses, etc.), detection of tumor mutations, detection of genetic diseases, and detection of individual genotypes. In various application fields, such as infectious disease diagnosis, the sensitivity and specificity of the nucleic acid detection technology are greatly superior to those of a colloidal gold/immunofluorescence method, and the method is a gold standard for various diagnoses.
Because the traditional nucleic acid detection technology almost completely depends on instruments, and has high requirements on sample treatment, detection environment and personnel quality; one conventional nucleic acid detection usually takes several hours to a longer time from the acquisition of a sample to the purification of nucleic acid, the configuration of a detection system, reaction to the interpretation of a result. These factors limit the realization of rapid detection in situ. Some reports show that a complete process from an original sample to a result is realized through technologies such as microfluidics and the like, and the method does not depend on specific environment and detection technicians, but the detection needs to be based on complex and expensive instruments, so that the cost is increased, and the rapid detection on site cannot be widely realized. For the small number of reported researches on the instrument-free nucleic acid field detection methods, which are really applied to field detection, there are some problems to be solved: one type of report is based on detection of color development by methods such as test paper amplification-microsphere/colloidal gold/membrane hybridization and the like. Although the technical means can realize the instrument-free nucleic acid amplification and nucleic acid detection result display, a purified nucleic acid sample is often required to be extracted in advance, and a field rapid solution for nucleic acid purification is not provided; the reports cannot be directly applied to the detection of various types of sample nucleic acids in a complicated and diversified way in the field. Another report provides a simple nucleic acid extraction device, and such a technique can be based on handheld filter membrane method extraction or magnetic bead method extraction, and then is docked with subsequent steps of lysis, reaction, detection and the like (paper-based reaction, and freeze-dried reagent redissolution reaction are many). The technology can realize extraction-amplification-detection processes without instruments, but has the disadvantages of complex operation, multiple steps, high error probability, high pollution risk and difficult wide application.
However, the existing nucleic acid detection cards, such as patent 201721028233.8, are still developed based on the traditional rapid nucleic acid detection technology (such as LAMP, RCA, RPA, etc.), and need to extract nucleic acids separately, and the detection process is complex to operate, and has many steps, is easy to make mistakes, has a large pollution risk, and is difficult to be widely applied.
In summary, the existing nucleic acid detection technology and detection card correspondingly have the problems of requirement of multiple steps of sample crude extraction, amplification, cutting, color development and the like, facing to the problems of uneven operator level, pollution risk and the like, or the problems of requirement of complex and expensive instruments, high cost, complex operation, multiple steps, easy error, high pollution risk and the like, can not realize field rapid detection widely, are difficult to popularize and apply, and further research and exploration are needed in the aspect of simply and conveniently realizing field rapid detection.
Disclosure of Invention
The to-be-solved technical problem of the utility model is to overcome above-mentioned prior art's defect and not enough, the purpose provides an instrument site short-term test card of exempting from that easy and simple to handle, do not rely on professional operating personnel, be difficult for polluting, the cost is lower. The detection card integrates all processes of sample extraction, amplification, result display and the like into one detection card, provides a field rapid solution for sample purification, does not need to extract a purified sample in advance, can be directly applied to field detection of various complicated and various samples, and realizes simple and convenient field rapid detection from an original sample to a result.
The utility model discloses above-mentioned purpose is realized through following technical scheme:
an instrument-free on-site rapid detection card comprises a sample inlet 1, a sample processing module 2, a reaction liquid flow path 5, a main reaction area 6 and a result area 10 which are sequentially arranged according to the flow direction of a sample, and is also provided with a puncture tube 3 which is linked with the sample processing module 2 and is internally provided with a cavity, and a reaction liquid cavity 4 which is adjacent to or contacted with the puncture tube 3; the sample processing module 2 moves and causes the puncture tube 3 to displace, and further causes the liquid in the reaction liquid cavity 4 to flow out through the inner cavity of the puncture tube 3 and flow through the sample processing module 2 into the sample flow path.
The utility model discloses a detection card can realize that original sample is simple and convenient to be detected to the result, during the use, add the sample by the application of sample mouth, after sample processing module 2's processing, control sample processing module 2 removes to make puncture pipe 3 take place the displacement, puncture pipe 3's displacement can cause 4 inside liquid in reaction liquid chamber to flow by puncture pipe 3 inside cavities, and pass through sample processing module 2, reaction liquid flow path 5 in proper order, enter into main reaction zone 6 and react, the result is presented in result district 10.
Furthermore, the sample processing module 2 is provided with a sample processing hole 22, a first guide surface 23 and a half-open drainage groove 21 penetrating through the sample processing hole 22; after the sample processing module 2 is moved, the cavity of the puncture tube 3 communicates with the sample processing well 22 and the reaction solution channel 5.
Meanwhile, the end of the puncture tube 2 is provided with a second guide surface that is engaged with the first guide surface 23. The half-open drainage groove 21 is used as a track for the sample processing module (2) to slide towards the puncture tube (3), and simultaneously provides a drainage function in the process that the sample processing module 2 slides towards the puncture tube 3, so that in the process of pushing the sample processing module 2, liquid in the sample processing module 4 flows back to the cavity 22 in the sample processing module 3, flows through the cavity 5 in the sample processing module 22 and then directly flows into the sample processing module 6, and meanwhile, reaction liquid in the sample processing module 4 cannot leak to other places of the detection card.
Preferably, the detection card is provided with a track for the movement of the sample processing module 2, which can be used to control the movement direction and distance of the sample processing module 2, and prevent the movement from deviating or moving too much.
Preferably, half-open drain groove 21 has n-way, where n is an integer of 1 or more. Meanwhile, the reaction liquid cavity 4, the sample processing hole 22 and the reaction regions can be arranged in parallel, correspondingly, the reaction liquid flow path 5 can be a single-tube channel or a multi-channel, and provides shunting, so that liquid enters the plurality of reaction regions in parallel, and multiple detection is realized. Therefore, the utility model discloses a detection card correspondence can be used to singly organize the detection, also can be used to the multiunit as multiple detection.
It is also preferred that the test card is further provided with a waste zone 13 in communication with the sample processing module 2. In the original state, the sample processing block 2 (sample processing hole 22) communicates with the waste liquid region 13, and after the sample processing block 2 is moved, the sample processing block 2 (sample processing hole 22) communicates with the cavity of the puncture tube 3 and the reaction liquid channel 5, respectively.
In order to adapt to the detection of multiple reaction steps, n second reaction zones 8 and corresponding n second reaction zone driving components 9 can be arranged between the main reaction zone 6 and the result zone 10, wherein n is not less than 0 and is an integer. For example: the detection of two-step reaction is needed, a main reaction area and a second reaction area are needed to be arranged, and a driving component of the second reaction area is correspondingly arranged; after the reaction in the main reaction zone is finished, the driving component of the second reaction zone drives the second reaction zone to contact with the main reaction zone, and the sample is transferred from the main reaction zone to the second reaction zone to continue the second-step reaction. If the detection of multi-step reaction is required, and so on, a plurality of second reaction zones and driving components thereof are designed in a serial mode.
In a specific alternative, the reaction zones are composed of strips, and the driving member is used for driving the strips in the second reaction zone to move to contact with the strips in the main reaction zone, so that the liquid can pass through the chromatographic column after the strips contact with each other.
In addition, the detection card is preferably further provided with a housing 12, and the housing 12 is provided with a control button for controlling the movement of the sample processing module 2. Preferably, the housing 12 is further provided with a control knob for controlling the second reaction zone driving assembly 9.
Preferably, the detection card is further provided with a result driving assembly 11 for controlling the result zone 10 to contact the main reaction zone 6. Preferably, the housing 12 is further provided with a control knob for controlling the result driving assembly 11.
When the detection card is a nucleic acid detection card, the result region 10 may be displayed by various methods, such as a colloidal gold method, a fluorescence method, a color change reaction, a hybridization method, or a microsphere method.
Preferably, the housing 12 is further provided with a control button for controlling the result driving assembly 11 and a result display area corresponding to the result area 10.
Preferably, the detection card is also provided with a temperature control zone 7 connected to the main reaction zone 6. The temperature control region 7 can be a heating material (such as iron powder, quicklime, a mixture of a plurality of heating materials and the like) or various types of electric heating devices.
Preferably, the reaction chamber 4 is made of a material that can be punctured, and the movement 2 can move the 3 upward to puncture 4. Or a valve for controlling the liquid to flow out is arranged on the reaction liquid cavity 4, and the movement 2 can push the movement 3 to move upwards to open the valve.
More preferably, an auxiliary pressing component 24 linked with the sample processing module 2 is further provided adjacent to the reaction liquid chamber 4 for assisting the liquid in the reaction liquid chamber 4 to flow out. The design of the auxiliary squeezing assembly 24 in mechanical linkage with the sample processing module 2 allows the auxiliary squeezing assembly 24 to squeeze the liquid 4 to assist in the squeezing out of the liquid in the sample processing module 4 while the movement 2 allows the abutting liquids 3 and 4 to flow out.
As an alternative case: the structural design of the auxiliary extrusion assembly 24 is as follows:
the auxiliary extrusion component 24 is located near the reaction liquid cavity 4 and connected with the control button of the sample processing module 2 (such as an integrated fixed connection), and when the control button of the sample processing module 2 is pushed, the auxiliary extrusion component 24 is driven to shift towards the direction close to the reaction liquid cavity 4, so that the auxiliary extrusion reaction liquid cavity 4 is assisted to extrude liquid therein.
As an alternative case, when the detection card is a nucleic acid detection card, a filter membrane is disposed in the sample processing hole 22, and the filter membrane is a nucleic acid extraction filter membrane, a silica gel membrane, a nucleic acid adsorption membrane, a nitrocellulose membrane, filter paper, glass fiber paper, or the like.
The shape of the injection port 1 is not limited, and may be any shape. The shape of the housing 12 is not limited and may be any shape.
The utility model discloses a principle and the application method flow of detection card are as follows:
the first step is as follows: when a liquid sample or a pretreated liquid sample is added into the detection card from the sample inlet 1, the liquid in the sample flows into the waste liquid region 13 through the sample processing module 2, and the substance to be extracted (such as nucleic acid) in the sample is retained in the sample processing hole 22 of the sample processing module 2.
The second step is that: the push 2 moves forwards and 3 moves relatively, 3 moves upwards through the first guide surface matched with the first guide surface and the second guide surface, 3 can puncture the reaction liquid cavity 4 encapsulated by the pierceable material or open a valve of the reaction liquid cavity 4 after moving upwards, the reaction liquid in 4 flows out from the middle cavity of 3, finally flows back to 22 through the half-open drainage groove 21 along with the process of pushing 2, then flows back to 5 through 22 and then directly flows into 6.
The third step: the sample and the reaction liquid react in the reaction area: reaction in 6, zone 7 may provide temperature control (e.g., heating). (if the reaction is two-step reaction, the first-step reaction is firstly completed in the first reaction zone, then the second reaction zone is driven to be contacted with the first reaction zone by moving the 9, and the transfer of the sample from the first reaction zone to the second reaction zone is completed.) if the reaction is three-step reaction zone, the reasoning is carried out in sequence.
The fourth step: after the reaction is completed, the result driving member 11 is moved to bring the result area 10 into contact with the reaction area, so that the sample in the completed reaction area enters the result area 10 to obtain a result.
The utility model discloses following beneficial effect has:
the utility model discloses an instrument site of exempting from short-term test card need not to extract the purification sample in advance, can directly apply to the complicated various types of sample detection of scene, has realized the simple and convenient on-the-spot short-term test from original sample to the result, and does not rely on large-scale or expensive instrument, cost lower.
The utility model discloses a detection card is easy and simple to handle, quick, be difficult for makeing mistakes, only needs non-professional person two steps of operations, has reduced the mistake and the error that different operators brought by a wide margin, has also avoided the risk of aerosol pollution, and is all not high to sample treatment, detection ring border and personnel's quality requirement, and the pollution risk is little, false positive rate is extremely low.
The utility model discloses a detection card not only is applicable to the on-the-spot short-term test of nucleic acid, also is applicable to the complicated detection reaction of multiple similar traditional step, and application scope is wide, and application prospect is good, has fine value to on-the-spot short-term test such as nucleic acid.
Drawings
Fig. 1 is the schematic diagram of the internal structure of the detection card of the present invention.
Fig. 2 is a partially enlarged view of the sample processing block 2.
FIG. 3 is an enlarged view of a portion of the test card in the area of the sample processing module 2, the piercing tube 3 and the reaction solution chamber 4.
Fig. 4 is a partial structural view of the auxiliary pressing member 24 in the test card.
The reference numbers in the figures are: 1 introduction port, 2 sample processing modules, 3 puncture tubes, 4 reaction liquid cavities, 5 reaction liquid flow paths, 6 main reaction areas, 7 temperature control areas, 8 second reaction areas, 9 second reaction area driving components, 10 result areas, 11 result driving components, 12 shells, 13 waste liquid areas, 21 half-open drainage grooves, 22 sample processing holes, 23 first guide surfaces and 24 auxiliary extrusion components.
Detailed Description
The invention is further described with reference to the drawings and the following examples, which are not intended to limit the invention in any way. Unless otherwise indicated, the reagents, methods and apparatus employed in the present invention are conventional in the art.
Unless otherwise indicated, reagents and materials used in the present invention are commercially available.
EXAMPLE 1 Instrument-free on-site quick test card
1. An instrument-free on-site rapid detection card is shown in figures 1-3 and comprises a sample inlet 1, a sample processing module 2, a reaction liquid flow path 5, a main reaction area 6, a second reaction area 8, a corresponding second reaction area driving component 9 and a result area 10 which are sequentially arranged according to the flow direction of a sample; a puncture tube 3 with a cavity inside and a reaction liquid cavity 4 adjacent to or in contact with the puncture tube 3 are also arranged; the device also comprises a result driving assembly 11, a shell 12 and a waste liquid area 13.
Wherein, the sample inlet 1 is communicated with the sample processing module 2; the sample processing module 2 can move, the sample processing module 2 is in contact with the puncture tube 3 and can slide between the sample processing module 2 and the puncture tube 3, and the sample processing module 2 is pushed to enable the puncture tube 3 to be displaced; puncture pipe 3 is adjacent with reaction liquid chamber 4, and puncture pipe 3 contact with reaction liquid chamber 4 after the displacement and arouse that reaction liquid chamber 4 inside liquid flows out.
Specifically, the sample processing module 2 is provided with a sample processing hole 22, a first guide surface 23 and a half-open drainage groove 21. The reaction solution channel 5 connects the sample processing well 22 and the main reaction region 6.
The detection card is internally provided with a track for the movement of the sample processing module 2, and the track can be used for controlling the movement direction and distance of the sample processing module 2 and preventing the movement from deviating or moving too much.
For example, in the case of nucleic acid detection, a nucleic acid extraction filter is disposed in the sample processing well 22 for extracting nucleic acid samples.
The puncture tube 3 is internally provided with a cavity, one end face which is contacted with the sample processing module 2 is a second guide surface which is matched with the first guide surface, and the two surfaces can slide.
The semi-open drainage groove 21 is provided with a sliding rail for the sample processing module (2) to slide towards the puncture tube (3); the other end of the puncture tube 3 is adjacent to the reaction liquid cavity 4. The reaction liquid cavity 4 is made of a pierceable material, and the movement 2 can push the puncture 3 to move upwards to pierce the puncture 4 so as to make the reaction liquid in the reaction liquid cavity flow out.
The semi-open leakage groove 21 not only provides a sliding rail for the sample processing module (2) to slide towards the puncture tube (3), but also provides a leakage function during the sliding process of the sample processing module 2 towards the puncture tube 3. During the pushing 2, the liquid in the 3 triggering 4 flows back to the 22 through the inner cavity of the 3, flows through the 5 from the 22 and then flows into the 6 directly. And meanwhile, the reaction liquid in the step 4 is ensured not to leak to other places of the detection card.
In addition, the result zone 10 is adjacent to the second reaction zone 8, and the result driving assembly 11 controls the contact of the result zone 10 and the second reaction zone 8; the waste zone 13 is in communication with the sample processing module 2.
The main reaction zone 6 and the second reaction zone 8 are both composed of test strips, the driving component is used for driving the test strips in the second reaction zone to move and contact with the test strips in the main reaction zone, and after the test strips contact with each other, liquid can pass through the chromatography.
The display method of the result area 10 is a colloidal gold method.
The housing 12 is provided with a control knob for controlling the movement of the sample processing module 2.
The housing 12 is also provided with a control knob for controlling the second reaction zone driving assembly 9.
The housing 12 is also provided with a control knob for controlling the result drive assembly 11.
The housing 12 is also provided with a display area corresponding to the result area.
2. The principle and the using method of the detection card have the following flows:
the first step is as follows: a liquid sample or a pretreated liquid sample is added to the detection card from 1, the liquid in the sample flows into 13 through 2, and substances (such as nucleic acid) to be extracted in the sample are left on the filter membrane of 2.
The second step is that: the push 2 moves forwards and 3 moves relatively, the 3 moves into the half-open drainage groove 21 through the first guide surface 23 to promote the 3 to move upwards, the 3 moves upwards to puncture the reaction liquid in the reaction liquid cavity 4, the reaction liquid flows out from the middle cavity of the 3 and finally flows back to the 22 through the half-open drainage groove 21 along with the process of pushing 2, then flows through the 5 through the 22 and then directly flows into the 6.
The third step: the sample and the reaction liquid are reacted in 6. After the first-step reaction is finished in the reaction area 6, the movement 9 drives the second reaction area 8 to contact with the main reaction area 6, and the second-step reaction of the sample from the main reaction area 6 to the second reaction area 8 is finished.
The fourth step: after the second reaction step in the second reaction region 8 is completed, the module 11 is moved to bring the reaction region 10 into contact with the reaction region 8, thereby completing the sample introduction 10 into the reaction region 8, obtaining a result, and presenting a display region on the housing 12.
EXAMPLE 2 Instrument-free on-site quick detection card
An instrument-free on-site rapid detection card is shown in figures 1-4, the structure of the instrument-free on-site rapid detection card is the same as that of embodiment 1, an auxiliary extrusion assembly 24 and a temperature control area 7 can be further arranged at the position adjacent to a reaction liquid cavity 4, and the temperature control area 7 is made of heating materials such as iron powder, quicklime and a mixture of multiple heating materials and can provide temperature control requirements for the reaction area.
The auxiliary squeezing assembly 24 is mechanically linked with the sample processing module 2 and is used for assisting the liquid in the reaction liquid cavity 4 to flow out. It is achieved that the pressing 24 of the 4 assists the liquid in the 4 to be squeezed out, at the same time as the movement 2 causes the 3 to move up to pierce the 4 so that the liquid therein flows out.
Specifically, the structure of the auxiliary squeezing assembly 24 is shown in fig. 4:
the auxiliary extrusion component 24 is located near the reaction liquid cavity 4 and connected with the control button of the sample processing module 2 (such as an integrated fixed connection), and when the control button of the sample processing module 2 is pushed, the auxiliary extrusion component 24 is driven to shift towards the direction close to the reaction liquid cavity 4, so that the auxiliary extrusion reaction liquid cavity 4 is assisted to extrude liquid therein.
The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be equivalent replacement modes, and all are included in the scope of the present invention.
Claims (15)
1. An instrument-free on-site rapid detection card is characterized by comprising a sample inlet (1), a sample processing module (2), a reaction liquid flow path (5), a main reaction area (6) and a result area (10) which are sequentially arranged according to the flow direction of a sample, and further comprising a puncture tube (3) which is linked with the sample processing module (2) and is internally provided with a cavity, and a reaction liquid cavity (4) which is adjacent to or contacted with the puncture tube (3); the sample processing module (2) moves and causes the puncture tube (3) to displace, and further causes liquid in the reaction liquid cavity (4) to flow out through the inner cavity of the puncture tube (3) and flow through the sample processing module (2) to enter the sample flow path.
2. The test card of claim 1, wherein the sample processing module (2) is provided with a sample processing hole (22), a first guide surface (23), a semi-open drainage groove (21) extending through the sample processing hole (22); the semi-open drainage groove (21) is simultaneously used as a track for the sample processing module (2) to slide towards the puncture tube (3); after the sample processing module (2) is moved, the cavity of the puncture tube (3) is communicated with the sample processing hole (22) and the reaction solution flow path (5).
3. The test card according to claim 2, characterized in that the end of the puncture tube (3) is provided with a second guide surface cooperating with the first guide surface (23).
4. The test card according to claim 1 or 2, characterized in that the test card is internally provided with a track for the movement of the sample processing modules (2).
5. The test card according to claim 1 or 2, further provided with a waste zone (13) communicating with the sample processing module (2).
6. The test card of claim 1, further comprising a housing (12), wherein the housing (12) is provided with a control knob for controlling the movement of the sample processing module (2).
7. The test card according to claim 1, further comprising a result driver assembly (11) for controlling the contact of the result zone (10) with the main reaction zone (6).
8. The test card of claim 7, further comprising a housing (12), wherein the housing (12) is provided with a control button for controlling the result driving unit (11) and a result display area corresponding to the result area (10).
9. The test card of claim 1, wherein n second reaction zones (8) and corresponding n second reaction zone driving assemblies (9) are further disposed between the main reaction zone (6) and the result zone (10), wherein n is an integer greater than or equal to 0.
10. The test card of claim 9, further comprising a housing (12), wherein the housing (12) further comprises a control knob for controlling the second reaction region driving unit (9).
11. The test card according to claim 1, further comprising a temperature control zone (7) connected to the main reaction zone (6).
12. The test card of claim 2, wherein when the test card is a nucleic acid test card, a filter membrane is disposed in the sample processing well (22), and the filter membrane is a nucleic acid extraction filter membrane, a silica gel membrane, a nucleic acid adsorption membrane, a nitrocellulose membrane, filter paper, or glass fiber paper.
13. The test card of claim 2, wherein the half-open vent groove (21) has n channels, n being an integer of 1 or more.
14. The test card of claim 1, wherein the reaction liquid chamber (4) is made of a pierceable material, or the reaction liquid chamber (4) is provided with a valve for controlling the outflow of liquid.
15. The test card of claim 14, wherein an auxiliary squeezing assembly (24) is provided adjacent to the reaction chamber (4) and is operatively associated with the sample processing module (2).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110241021A (en) * | 2019-05-23 | 2019-09-17 | 广州普世利华科技有限公司 | One kind exempting from instrument nucleic acid on-site quick detection kit |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110241021A (en) * | 2019-05-23 | 2019-09-17 | 广州普世利华科技有限公司 | One kind exempting from instrument nucleic acid on-site quick detection kit |
| CN110241021B (en) * | 2019-05-23 | 2022-04-12 | 广州普世利华科技有限公司 | Instrument-free nucleic acid on-site rapid detection kit |
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