CN210596067U - Cell disruption and separation device - Google Patents
Cell disruption and separation device Download PDFInfo
- Publication number
- CN210596067U CN210596067U CN201921280939.2U CN201921280939U CN210596067U CN 210596067 U CN210596067 U CN 210596067U CN 201921280939 U CN201921280939 U CN 201921280939U CN 210596067 U CN210596067 U CN 210596067U
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- cup
- shell
- cover
- containing cup
- separation device
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Abstract
The utility model discloses a cell crushing and separating device, which comprises a shell, a top cover, a stirring machine head and a stirring rod; the top cover is movably covered on the shell; the stirring machine head is arranged on the top cover; the stirring rod is inserted into the shell and is driven to rotate by the stirring machine head; a containing cup is movably arranged at the bottom of the shell; an opening for taking out the containing cup is formed in the side wall of the shell; the cup cover is movably arranged on the containing cup; the lower surface of the cup cover is connected with a connecting rod; the end part of the connecting rod is fixedly connected with a filter plate; the outer diameter of the filter plate is matched with the inner diameter of the containing cup; the cup cover is also provided with a liquid taking hole; a sealing cover is hinged on the liquid taking hole; the utility model discloses the technical scheme who takes has solved current broken separator splendid attire cup and has dismantled and wash inconvenient problem, and filters, takes a sample problem of longer influence experiment effect consuming time.
Description
Technical Field
The utility model relates to a molecular biology experiment operating equipment technical field, concretely relates to cell disruption separation device.
Background
The cell disruption technique is a technique for releasing cell contents by destroying cell membranes and cell walls by an external force. The cell disruption and separation extract a target content, firstly, the cell tissue is disrupted by a disruption mechanism, and then the separation and filtration are rapidly carried out to avoid the inactivation of the target content. Because the most fixed existence of splendid attire cup is dismantled among the broken separator of current cell and wash inconvenient problem, and need shift earlier the material in the splendid attire cup when filtering, taking a sample and lead to the problem of long influence experiment effect consuming time.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a cell crushing and separating device solves the inconvenient problem of current cell crushing and separating device splendid attire cup dismantlement and washing, and filters, takes a sample problem of longer influence experiment effect consuming time.
In order to solve the technical problem, the utility model adopts the following technical scheme: a cell crushing and separating device comprises a shell, a top cover, a stirring machine head and a stirring rod; the top cover is movably covered on the shell; the stirring machine head is arranged on the top cover; the stirring rod is inserted into the shell and is driven to rotate by the stirring machine head; a containing cup is movably arranged at the bottom of the shell; an opening for taking out the containing cup is formed in the side wall of the shell; the cup cover is movably arranged on the containing cup; the lower surface of the cup cover is connected with a connecting rod; the end part of the connecting rod is fixedly connected with a filter plate; the outer diameter of the filter plate is matched with the inner diameter of the containing cup; the cup cover is also provided with a liquid taking hole; a sealing cover is hinged on the liquid taking hole;
further, when the containing cup is placed in the shell, the containing cup is opened, and the stirring rod extends into the containing cup;
further, a base is arranged at the bottom of the containing cup; the base comprises a shock absorption seat and a mounting seat; a clamping ring is arranged on the side wall of the mounting seat;
furthermore, a groove is formed in the bottom of the shell corresponding to the base of the containing cup; a clamping groove matched with the clamping ring is arranged on the side wall of the groove; the clamping ring rotates and is locked in the clamping groove;
furthermore, a circle of flange part is arranged at the edge of the filter plate; the flange part extends upwards to form a folded edge;
further, the flange part and the folded edge are both made of flexible materials;
furthermore, a sealing retaining ring extends downwards from the edge of the cup cover;
a side door is hinged on the opening; and an observation window is also arranged on the side door.
Compared with the prior art, the beneficial effects of the utility model are one of following at least:
1. the top cover is movably arranged on the shell, the stirring machine head is arranged on the top cover and is connected with the stirring rod, and the stirring rod can be taken out or extended into the shell by opening or closing the top cover on the shell to crush and stir the cells in the containing cup;
2. after the containing cup is movably arranged at the bottom of the shell, the containing cup can be quickly taken out through the opening on the side wall of the shell;
3. after the containing cup is taken out, the cup cover is vertically covered downwards, the cup cover pushes the connecting rod and the filter plate to move towards the bottom of the containing cup, the inside of the containing cup is easy to filter and separate, so that cell sap containing small molecules enters the upper part of the containing cup through micropores on the filter plate, cell outer wall tissues are isolated below the filter plate and gradually deposited at the bottom of the containing cup due to the downward pressure of the filter plate, and separation and filtration are completed;
4. during sampling, the sealing cover arranged on the cup cover is opened, and cell supernatant is taken out through the liquid taking hole and the dropper for subsequent experimental analysis and operation; after sampling, the sample is covered with a sealing cover and sent into the constant temperature box, so that the sample is convenient to store.
5. The utility model discloses dismouting separation splendid attire cup is convenient, does benefit to cleanly, can carry out the filtering separation to broken result fast simultaneously, shifts the loss of material in the middle of reducing, improves the efficiency of operation and the degree of accuracy of experiment.
The utility model discloses a working process does: filling a proper amount of operation cell tissues and extractant into the containing cup, and putting the containing cup into the shell through the opening after the top cover is opened; after aligning the base of the containing cup with the groove at the bottom of the shell, vertically and downwards inserting the containing cup into the groove, and then rotating the containing cup to lock the clamping ring in the clamping groove; then closing the side door of the shell; after the top cover is vertically covered, the stirring rod extends into the containing cup, the stirring machine head is started, and the materials in the containing cup are crushed and stirred; after the crushing, open the side door, take out the splendid attire cup, cover the bowl cover downwards from vertical direction fast for the filter plate is vertical downwards along the splendid attire cup axial, to the separation and the filtration of material in the splendid attire cup. After the cup cover is buckled, standing for a proper time, and opening a sealing cover on the liquid taking hole for sampling and analyzing.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
FIG. 2 is a schematic view of the structure of the container.
Fig. 3 is a top view of the cup lid.
Fig. 4 is a bottom plan view of the housing.
In the drawings: 1. a housing; 2. a top cover; 3. a stirring machine head; 4. a stirring rod; 5. a containing cup; 6. an opening; 7. a cup cover; 8. a connecting rod; 9. filtering the plate; 10. a liquid extraction hole; 11. a sealing cover; 12. a shock absorbing seat; 13. a mounting seat; 14. a collar; 15. a groove; 16. a flange portion; 17. folding edges; 18. a sealing snap ring; 19. a side door; 20. and (4) an observation window.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1: as shown in FIGS. 1 to 4, a cell disruption and separation device comprises a housing 1, a top cover 2, a stirrer head 3 and a stirring rod 4; the top cover 2 is movably covered on the shell 1; the stirrer head 3 is arranged on the top cover 2; the stirring rod 4 is inserted into the shell 1 and is driven to rotate by the stirring head 3; a containing cup 5 is movably arranged at the bottom of the shell 1; an opening 6 for taking out the containing cup 5 is formed in the side wall of the shell 1; the cup cover 7 is movably arranged on the containing cup 5; the lower surface of the cup cover 7 is connected with a connecting rod 8; the end part of the connecting rod 8 is fixedly connected with a filter plate 9; the outer diameter of the filter plate 9 is matched with the inner diameter of the containing cup 5; the cup cover 7 is also provided with a liquid taking hole 10; a sealing cover 11 is hinged on the liquid taking hole 10; in this embodiment, the stirring head 3 is a conventional electric stirring head 3 and is electrically connected to an external power source. The electric mixer head 3 with any structure and reasonable connection can be selected. In this embodiment, the output shaft of puddler 4 and electric mixer head 3 is connected the back, passes and sets up top cap 2, vertically stretches into in the casing 1. The top cover 2 can be provided with a through hole for the stirring rod 4 to pass through, and a bearing piece with a proper structure is arranged to ensure the stable rotation; the stirring rod 4 can be provided with a conventional crushing blade or a glass ball.
The top cover 2 is movably arranged on the shell 1, the stirring machine head 3 is arranged on the top cover 2 and is connected with the stirring rod 4, the stirring rod 4 can be taken out or stretched into the shell 1 by opening or closing the top cover 2 on the shell 1, and cells in the containing cup 5 are crushed and stirred; after the containing cup 5 is movably arranged at the bottom of the shell 1, the containing cup 5 can be quickly taken out through the opening 6 on the side wall of the shell 1; after the containing cup 5 is taken out, the cup cover 7 is vertically covered downwards, the cup cover 7 pushes the connecting rod 8 and the filter plate 9 to move towards the bottom of the containing cup 5, the inside of the containing cup 5 is easy to filter and separate, so that cell sap containing small molecules enters the upper part of the containing cup 5 through micropores on the filter plate 9, the outer wall tissue of the cell is isolated below the filter plate 9, and is gradually deposited at the bottom of the containing cup 5 due to the downward pressure of the filter plate 9, and the separation and the filtration are completed; during sampling, the sealing cover arranged on the cup cover 7 is opened, and cell supernatant is taken out through the liquid taking hole 10 and the dropper for subsequent experimental analysis and operation; after sampling, covering a sealing cover 11 and sending the sample into a constant temperature box for convenient storage; the utility model discloses dismouting separation splendid attire cup 5 is convenient, does benefit to cleanly, can carry out the filtration separation to broken result fast simultaneously, reduces the loss of middle transfer material, improves the efficiency of operation and the degree of accuracy of experiment
When the containing cup 5 is placed in the shell 1, the containing cup 5 is opened, and the stirring rod 4 extends into the containing cup 5; when splendid attire cup 5 was located casing 1, taken off bowl cover 7 and made 5 upper ends of splendid attire cup uncovered, the puddler 4 of being convenient for stretched into in the splendid attire cup 5 and carries out broken stirring to the material in the splendid attire cup 5. When the containing cup 5 is taken out of the shell 1, the cup cover 7 is covered again, and filtering separation, sealing and sampling of the content of the cup cover 7 are carried out.
The bottom of the containing cup 5 is provided with a base; the base comprises a shock absorption seat 12 and a mounting seat 13; a clamping ring 14 is arranged on the side wall of the mounting seat 13; in this embodiment, cushion socket 12 and splendid attire cup 5 lug connection, cushion socket 12 adopt conventional rubber material, play the effect of buffering, and mount pad 13 holds cushion socket 12 for fixed and installation splendid attire cup 5. A collar 14 at the bottom of the mounting 13 is used to snap the container cup 5 onto the bottom of the housing 1.
The bottom of the shell 1 is provided with a groove 15 corresponding to the base of the containing cup 5; a clamping groove matched with the clamping ring 14 is formed in the side wall of the groove 15; the clamping ring 14 is rotationally locked in the clamping groove; the groove 15 is used for clamping the base, the base is aligned to the groove 15, then the base is axially inserted into the groove 15, the containing cup 5 is rotated, the clamping ring 14 on the mounting seat 13 can be locked in the clamping groove, and the containing cup 5 is stably fixed.
A circle of flange parts 16 are arranged at the edge of the filter plate 9; the flange part 16 extends upwards to form a folded edge 17; the flange part 16 at the edge of the filter plate 9 is contacted with the cup wall of the containing cup 5, the sealing effect is enhanced, and the folding edge 17 enhances the sealing effect.
The flange portion 16 and the raised portion are both of flexible material; in this embodiment, flange portion 16 and bellying all adopt flexible metal filter screen material, can strengthen the sealed effect at filter plate 9 edge, avoid organizing the thing come-up.
A sealing snap ring 18 extends downwards from the edge of the cup cover 7; the retaining ring enhances the sealing effect of the cup cover 7 on the interior of the containing cup 5, and can be connected with the cup body of the containing cup 5 by adopting any reasonable clamping or other modes.
A side door 19 is hinged on the opening 6; the side door 19 is also provided with an observation window 20; the side door 19 can seal the inside of the casing 1 during crushing, so that the cells are prevented from being polluted, and the observation window 20 is convenient for observing the working condition inside the casing 1. The side door 19 and the edge of the opening 6 can adopt any reasonable hinging mode and connecting structure; the edge of the side door 19 and the edge of the opening 6 can be movably connected through a conventional lock head and a lock tongue.
The utility model discloses a working process does: the holding cup 5 is filled with a proper amount of operation cell tissues and extractant, and after the top cover 2 is opened, the holding cup 5 is placed into the shell 1 through the opening 6; after aligning the base of the containing cup 5 with the groove 15 at the bottom of the shell 1, vertically inserting the containing cup 5 into the groove 15 downwards, and then rotating the containing cup 5 to lock the clamping ring 14 in the clamping groove; then closing the side door 19 of the case 1; after the top cover 2 is vertically covered, the stirring rod 4 extends into the containing cup 5, the stirring machine head 3 is started, and the materials in the containing cup 5 are crushed and stirred; after the crushing, open side door 19, take out splendid attire cup 5, cover bowl cover 7 from vertical direction downwards fast for filter plate 9 is vertical downwards along splendid attire cup 5 axial, to the separation and the filtration of the interior material of splendid attire cup 5. After the cup cover 7 is buckled, standing for a proper time, and opening the sealing cover 11 on the liquid taking hole 10 for sampling analysis.
Although the invention has been described herein with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the spirit and scope of the principles of this invention. More specifically, various variations and modifications are possible in the component parts and/or arrangements of the subject combination arrangement within the scope of the disclosure, the drawings and the appended claims. In addition to variations and modifications in the component parts and/or arrangements, other uses will also be apparent to those skilled in the art.
Claims (8)
1. A cell crushing and separating device comprises a shell (1), a top cover (2), a stirrer head (3) and a stirring rod (4); the top cover (2) is movably covered on the shell (1); the stirring machine head (3) is arranged on the top cover (2); the stirring rod (4) is inserted into the shell (1) and is driven to rotate by the stirring head (3); the method is characterized in that: a containing cup (5) is movably arranged at the bottom of the shell (1); an opening (6) for taking out the containing cup (5) is formed in the side wall of the shell (1); a cup cover (7) is movably arranged on the containing cup (5); the lower surface of the cup cover (7) is connected with a connecting rod (8); the end part of the connecting rod (8) is fixedly connected with a filter plate (9); the outer diameter of the filter plate (9) is matched with the inner diameter of the containing cup (5); the cup cover (7) is also provided with a liquid taking hole (10); the liquid taking hole (10) is hinged with a sealing cover (11).
2. The cell disruption and separation device according to claim 1, wherein: when the containing cup (5) is placed in the shell (1), the containing cup (5) is opened, and the stirring rod (4) extends into the containing cup (5).
3. The cell disruption and separation device according to claim 1, wherein: the bottom of the containing cup (5) is provided with a base; the base comprises a shock absorption seat (12) and a mounting seat (13); a clamping ring (14) is arranged on the side wall of the mounting seat (13).
4. The cell disruption and separation device according to claim 3, wherein: a groove (15) is formed in the bottom of the shell (1) corresponding to the base of the containing cup (5); a clamping groove matched with the clamping ring (14) is formed in the side wall of the groove (15); the clamping ring (14) is rotationally locked in the clamping groove.
5. The cell disruption and separation device according to claim 1, wherein: a circle of flange parts (16) are arranged at the edge of the filter plate (9); a folded edge (17) extends upwards from the flange part (16).
6. The cell disruption and separation device according to claim 5, wherein: the flange part (16) and the folded edge (17) are both made of flexible materials.
7. The cell disruption and separation device according to claim 1, wherein: the edge of the cup cover (7) extends downwards to form a sealing snap ring (18).
8. The cell disruption and separation device according to claim 1, wherein: a side door (19) is hinged to the opening (6); the side door (19) is also provided with an observation window (20).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921280939.2U CN210596067U (en) | 2019-08-08 | 2019-08-08 | Cell disruption and separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921280939.2U CN210596067U (en) | 2019-08-08 | 2019-08-08 | Cell disruption and separation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210596067U true CN210596067U (en) | 2020-05-22 |
Family
ID=70686185
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921280939.2U Expired - Fee Related CN210596067U (en) | 2019-08-08 | 2019-08-08 | Cell disruption and separation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210596067U (en) |
-
2019
- 2019-08-08 CN CN201921280939.2U patent/CN210596067U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200522 Termination date: 20210808 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |