CN210560495U - Fish environment DNA moves water and returns to trend characteristic test device - Google Patents
Fish environment DNA moves water and returns to trend characteristic test device Download PDFInfo
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- CN210560495U CN210560495U CN201921138548.7U CN201921138548U CN210560495U CN 210560495 U CN210560495 U CN 210560495U CN 201921138548 U CN201921138548 U CN 201921138548U CN 210560495 U CN210560495 U CN 210560495U
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Abstract
The utility model provides a fish environment DNA moves water and returns to trend characteristic test device, including the annular runner that supplies fish to perch, be located the frequency conversion disc of annular runner one side and make a stream system, set up in the frequency conversion disc and make upstream fish grating and the low reaches fish grating of stream system upper and lower reaches, be located the water environment factor monitoring system, ADV current meter and the sampling system of annular runner opposite side, the frequency conversion disc is made a stream system and is included inverter motor, drive belt, axis of rotation and disc piece, inverter motor passes through the drive of drive belt axis of rotation and then drives the disc piece rotation. The utility model discloses can simulate the unlimited length river course of different velocity of flow, satisfy environment DNA and move water and return to characteristic test needs, simple and easy, easy operation has great using value.
Description
Technical Field
The utility model relates to a water environment and water ecological protection field specifically indicate a fish environment DNA moves water and returns to trend characteristic testing arrangement.
Background
The DNA monitoring of fish environment in water body applies qualitative analysis of fish community composition characteristics and the like, but quantitative analysis of application and resource quantity prediction analysis and the like needs to master the tendency characteristics of environmental DNA shedding, degradation, adsorption and the like. The environmental DNA is influenced by various environmental factors such as water power, water temperature and dissolved oxygen, and the quantitative relation between the environmental factors and the environmental DNA homing characteristics is difficult to establish.
SUMMERY OF THE UTILITY MODEL
The utility model aims at overcoming the defects of the background art and providing a device for testing the water-moving tendency and tendency characteristics of the DNA of a fish environment.
In order to achieve the above object, the utility model provides a fish environment DNA moves water and returns to trend characteristic test method which is characterized in that, including the following steps:
the utility model provides a fish environment DNA moves water and returns to trend characteristic test device, includes that the annular flow way that supplies fish to perch, the frequency conversion disc that is located annular flow way one side make the stream system, set up in upstream barricade and the low reaches barricade of frequency conversion disc system upper and lower reaches, be located the water environmental factor monitoring system of annular flow way opposite side, ADV velocity of flow appearance and sampling system, the frequency conversion disc makes the stream system and includes inverter motor, drive belt, axis of rotation and disc piece, inverter motor passes through the drive axis of rotation of drive belt and then drives the disc piece rotatory.
Furthermore, the arrangement width of the disc sheets is close to the width of the annular flow channel, and the distance between the disc sheets is set to be 0.5-2 cm.
Furthermore, the annular flow channel is made of organic glass.
Further, the annular flow passage is circular or elliptical.
Furthermore, the upstream fish blocking fence is made of a round pipe with the diameter of 0.5-2 cm and is made into a honeycomb structure, the downstream fish blocking fence is made of chemical fiber net sheets, and the mesh size can prevent the tested fishes from passing through.
Furthermore, a positioning support is arranged at the upper end of the annular flow channel, the water environment factor monitoring system and the ADV flow velocity meter are erected on the positioning support, and the ADV flow velocity meter is used for measuring the flow velocity and the turbulence characteristics of different spatial positions of the water body.
Furthermore, the water environment factor monitoring system comprises a water temperature sensor, a dissolved oxygen sensor, a pH value sensor, a conductivity sensor and a turbidity sensor, and is used for monitoring the change process of the water environment factor.
Furthermore, the sampling system sets up a plurality of sample connection along the depth of water, and the sample connection sets up the sample valve for take the water sample at different time interval, test environment DNA concentration.
The utility model discloses following beneficial effect has:
1. the utility model adopts the annular flow passage to simulate the water flow of the infinite long river channel in a limited space range, and meets the space-time requirement of the environmental DNA dynamic water regression characteristic test;
2. the utility model adopts the frequency conversion disc flow making system to form different hydrodynamic conditions, and a gap is left between the disc sheets, thus not generating mechanical damage to the environmental DNA fragments;
3. the utility model takes the difference of the width and the depth of the annular flow passage into consideration, and a full-section sampling system is arranged, so that the sampling uniformity can be ensured;
4. the utility model discloses deployed water environment factor monitoring system and ADV velocity of flow appearance, can quantitative test water environment factor and hydrodynamic force influence down environmental DNA's the characteristic of becoming trend.
Drawings
FIG. 1 is a schematic plane structure diagram of one embodiment of the device for testing the behavior of DNA kinetic water in fish environment according to the present invention;
3 FIG. 3 2 3 is 3 a 3 cross 3- 3 sectional 3 view 3 taken 3 along 3 line 3 A 3- 3 A 3 of 3 FIG. 3 1 3; 3
FIG. 3 is a schematic view of the cross-sectional structure B-B in FIG. 1.
In the figure: 1-an annular flow channel, 2-a variable frequency motor, 3-a transmission belt, 4-a rotating shaft, 5-a disc, 6-an upstream fish barrage, 7-a downstream fish barrage, 8-a positioning bracket, 9-an environmental factor monitoring system, 10-an ADV flow meter, 11-a sampling system, 12-a sampling port and 13-a sampling valve.
Detailed Description
The following detailed description of the preferred embodiments of the present invention will be given with reference to the accompanying drawings, which are not intended to limit the present invention, but are merely exemplary, so as to enable those skilled in the art to more clearly understand the contents and advantages of the present invention.
Referring to fig. 1-3, an embodiment of the device for testing the homing and homing characteristics of DNA flowing water in fish environment of the present invention, based on the principle that the water flow conditions in an annular flow channel are consistent in the air dimension, realizes the homing and homing characteristics of DNA flowing water in fish environment with high space-time characteristics in a limited space range, specifically comprises an annular flow channel 1 for fish to inhabit, a frequency conversion disc flow generating system located at one side of the annular flow channel 1, an upstream fish barrier 6 and a downstream fish barrier 7 arranged at the upstream and downstream of the frequency conversion disc flow generating system, a water environment factor monitoring system 9 located at the other side of the annular flow channel 1, an ADV flow velocity meter 10 and a sampling system 11, wherein the frequency conversion disc flow generating system comprises a frequency conversion motor 2, a transmission belt 3, a rotation shaft 4 and a disc 5, the frequency conversion motor 2 drives the rotation shaft 4 through the transmission belt 3 to drive the disc 5 to, the disc 5 is formed by overlapping a plurality of layers of discs up and down, the arrangement width of the disc 5 is close to that of the annular flow channel 1, the distance between the discs can be set to be 0.5-2 cm according to the flow making strength requirement, and the mechanical damage to the environmental DNA fragments can be avoided. The water environment factor monitoring system 9 comprises a water temperature (T, DEG C) sensor, a dissolved oxygen (DO, mg/L) sensor, a turbidity (Tur, NTU) sensor and the like, the ADV flow meter 10 is used for measuring the flow rate (u, m/s) and the turbulence characteristics of the water body, and the sampling system 11 is used for sampling water samples at different time intervals.
The annular flow channel 1 can be made of organic glass, so that the activity state of fishes can be conveniently observed, and environmental factors can be conveniently regulated and controlled; the shape of the test piece can be round or oval according to the test requirement.
The upstream fish barrier 6 can be made into a honeycomb structure by adopting a circular pipe with the diameter of 0.5-2 cm and has the function of adjusting the flow state of water flow; the downstream fish barrier 7 is made of chemical fiber net sheets, and the mesh size can prevent the tested fishes from passing through.
As shown in fig. 2, a positioning support 8 is arranged at the upper end of the annular flow channel 1, and the water environment factor monitoring system 9 is erected on the positioning support 8 and comprises water environment factor sensors for monitoring the change process of water environment factors, such as water temperature, dissolved oxygen, pH value, conductivity, turbidity and the like.
The ADV flow velocity meter 10 is also erected on the positioning bracket 8, so that the flow velocity and the turbulence characteristics of different spatial positions of the water body can be conveniently measured.
The utility model discloses the theory of operation: the biological sample of the living body of the target test fish collected from the field or a breeding field is placed into the annular flow channel 1, wherein the frequency disc flow generating system is positioned between the inner sides of the upstream fish barricade 6 and the downstream fish barricade 7, the biological sample of the living body of the target test fish is positioned between the outer sides of the upstream fish barricade 6 and the downstream fish barricade 7, then the variable frequency motor 2 is started, the rotating shaft of the variable frequency motor 2 is connected with the rotating shaft 4 through the driving belt 3, and then the rotating shaft 4 is driven to rotate, and as the disc 5 is arranged on the rotating shaft 4 in a penetrating mode, the disc. The running speed of the variable frequency motor 2 can be adjusted according to the test requirement, so that water flows with different flow rates are formed, a living biological sample of a target test fish moves between the outer sides of an upstream fish railing 6 and a downstream fish railing 7 in the annular flow channel 1, and the water environment factor monitoring system 9 and the ADV flow velocity meter 10 collect various water environment factor parameters such as water temperature, dissolved oxygen, pH value, conductivity and the like in real timeThe sampling port 12 in the sampling system 11 can take a quantitative water sample (V)imL), then performing vacuum filtration through a filter membrane with the aperture of 0.22 mu m, and storing the filtered filter membrane at-20 ℃ for later use, so as to prepare data for analyzing the time-varying rule of the water environment DNA concentration in the fate process of shedding, degradation, adsorption and the like, and further determine the shedding rate (S) and the degradation rate (k) of the fish environment DNA1) And adsorption ratio (k)2) And the like.
The above description is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are all covered by the protection scope of the present invention.
Claims (8)
1. The utility model provides a fish environment DNA moves water and returns to trend characteristic test device which characterized in that: the device comprises an annular flow channel for fish to inhabit, a variable frequency disc flow making system positioned on one side of the annular flow channel, an upstream fish barricade and a downstream fish barricade which are arranged on the upstream and downstream of the variable frequency disc flow making system, a water environment factor monitoring system positioned on the other side of the annular flow channel, an ADV (adaptive differential pressure) flow velocity meter and a sampling system, wherein the variable frequency disc flow making system comprises a variable frequency motor, a transmission belt, a rotating shaft and a disc, and the variable frequency motor drives the rotating shaft through the transmission belt so as to drive the disc to.
2. The device for testing the DNA kinetic water homing characteristics of the fish environment of claim 1, wherein: the arrangement width of the disc sheets is close to the width of the annular flow channel, and the distance between the disc sheets is set to be 0.5-2 cm.
3. The device for testing the DNA kinetic water homing characteristics of the fish environment of claim 1, wherein: the annular flow channel is made of organic glass.
4. The fish environment DNA kinetic water homing property test apparatus of claim 1 or 3, wherein: the annular flow passage is circular or elliptical.
5. The device for testing the DNA kinetic water homing characteristics of the fish environment of claim 1, wherein: the upstream fish blocking fence is of a honeycomb structure made of round pipes with the diameter of 0.5-2 cm, the downstream fish blocking fence is made of chemical fiber net sheets, and the size of the net holes can prevent tested fishes from passing through the net holes.
6. The device for testing the DNA kinetic water homing characteristics of the fish environment of claim 1, wherein: the upper end of the annular flow passage is provided with a positioning support, the water environment factor monitoring system and the ADV flow velocity meter are erected on the positioning support, and the ADV flow velocity meter is used for measuring the flow velocity and the turbulence characteristics of different spatial positions of the water body.
7. The device for testing the DNA kinetic water homing characteristics of fish environment according to claim 1 or 6, wherein: the water environment factor monitoring system comprises a water temperature sensor, a dissolved oxygen sensor, a pH value sensor, a conductivity sensor and a turbidity sensor and is used for monitoring the change process of the water environment factor.
8. The device for testing the DNA kinetic water homing characteristics of the fish environment of claim 1, wherein: the sampling system is provided with a plurality of sampling ports along the water depth, and the sampling ports are provided with sampling valves for sampling water samples at different time intervals and testing the DNA concentration of the environment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921138548.7U CN210560495U (en) | 2019-07-19 | 2019-07-19 | Fish environment DNA moves water and returns to trend characteristic test device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921138548.7U CN210560495U (en) | 2019-07-19 | 2019-07-19 | Fish environment DNA moves water and returns to trend characteristic test device |
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| Publication Number | Publication Date |
|---|---|
| CN210560495U true CN210560495U (en) | 2020-05-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921138548.7U Withdrawn - After Issue CN210560495U (en) | 2019-07-19 | 2019-07-19 | Fish environment DNA moves water and returns to trend characteristic test device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110305942A (en) * | 2019-07-19 | 2019-10-08 | 长江水利委员会长江科学院 | A kind of Fish Environment DNA moves water and returns become characteristic test method and device |
-
2019
- 2019-07-19 CN CN201921138548.7U patent/CN210560495U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110305942A (en) * | 2019-07-19 | 2019-10-08 | 长江水利委员会长江科学院 | A kind of Fish Environment DNA moves water and returns become characteristic test method and device |
| CN110305942B (en) * | 2019-07-19 | 2023-04-07 | 长江水利委员会长江科学院 | Method and device for testing DNA (deoxyribonucleic acid) dynamic water return-drive characteristic of fish environment |
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| GR01 | Patent grant | ||
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| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20200519 Effective date of abandoning: 20230407 |
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| AV01 | Patent right actively abandoned |
Granted publication date: 20200519 Effective date of abandoning: 20230407 |