CN210560363U - Cell membrane piece transfer device - Google Patents
Cell membrane piece transfer device Download PDFInfo
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- CN210560363U CN210560363U CN201920694130.8U CN201920694130U CN210560363U CN 210560363 U CN210560363 U CN 210560363U CN 201920694130 U CN201920694130 U CN 201920694130U CN 210560363 U CN210560363 U CN 210560363U
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- cell
- transfer device
- gel
- cell membrane
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Abstract
The utility model discloses a cell membrane transfer device, which comprises a bottom plate, a detachable transfer device and a cover body of the transfer device; the bottom plate is provided with a circular groove body, and gel is filled in the groove body; the gel is 7.5 wt% of pigskin gel; the transfer device comprises a cylindrical handle and a circular truncated cone in threaded connection with the handle; the cover body can cover the round platform and cover the round platform inside the cover body. The utility model provides a conveniently train cell membrane piece transfer device that cell transported, with the form transportation of cell with the diaphragm, avoided traditional cell digestion to use the damage that protease brought to cell and intercellular connection, kept cell autocrine's extracellular matrix and relevant protein and factor among the culture process, collected the cell with the complete membranous structure of one deck, improved the utilization ratio of cell, provide suitable microenvironment for the growth of internal cell.
Description
Technical Field
The utility model relates to the technical field of biological medical treatment, especially relate to a cell membrane piece transfer device.
Background
The basic principle of tissue engineering is to obtain a small amount of tissue from a body, culture and expand the obtained cells in vitro, mix the expanded cells with a biodegradable and absorbable biomaterial (scaffold) with good biocompatibility, adhere the cells to the biomaterial to form a cell-material complex, implant the complex into a tissue or organ lesion site in the body, continuously expand and secrete extracellular matrix in the body by the implanted cells, and finally form corresponding tissue or organ, thereby achieving the purposes of wound repair and reconstruction.
The traditional tissue engineering technical means mainly comprises cell scaffold material compounding and single cell suspension autologous or heterologous injection, and researches show that the traditional cell scaffold material strategy has some defects, such as large loss of inoculated cells and limited inoculation efficiency; cells are mainly adhered to the surface of the material and are unevenly distributed like a fishing net; conventional protease digestion for collecting cells is easy to change the phenotype and biochemical components of the cells, and often destroys extracellular matrix and intercellular junction protein. The recent emergence of cell patch technology has provided a new method for tissue regeneration. The cell patch technology is a technology for separating the amplified and fused cells from the bottom wall of a culture dish by a physical method to obtain a membrane-shaped cell structure. Compared with the traditional pancreatin digestion method, the cells collected by the technology retain the extracellular matrix secreted in the in vitro culture process, the established cell-matrix connection, the intercellular connection and other structures. It can collect the cell-extracellular matrix aggregate in the form of intact membrane, transport or adhere to the surface of the material, and become a good construction unit. The technology is used for successfully constructing tissues such as bones, cardiac muscles, corneas, esophageal epithelia and the like.
Although the cell membrane technology has the advantages of keeping transplanted cells moving and needing no scaffold material, the cell membrane technology has some defects at present, firstly, the single-layer cell membrane has poor mechanical performance, is relatively weak and fragile, is difficult to transfer and transport and is difficult to directly transplant, and therefore, a certain delivery vehicle is necessary for assisting transportation.
In view of the above, there is a need to design a cell membrane sheet transporting device that overcomes the above-mentioned technical problems.
SUMMERY OF THE UTILITY MODEL
The main object of the utility model is to provide a conveniently train cell membrane piece transfer device that cell transported can transport the cell with the form of diaphragm to guarantee the integrality of cell, improved its utilization ratio.
The purpose of the utility model and the technical problem thereof are realized by adopting the following technical scheme. According to the cell membrane sheet transfer device provided by the utility model,
comprises a bottom plate, a detachable transporter and a cover body of the transporter;
the bottom plate is provided with a circular groove body, and gel is filled in the groove body; the gel is 7.5 wt% of pigskin gel;
the transfer device comprises a cylindrical handle and a circular truncated cone in threaded connection with the handle;
the cover body can cover the round platform and cover the round platform inside the cover body.
The cell membrane sheet transfer device is characterized in that the transfer device, the bottom plate and the cover body are made of silicone materials;
the bottom plate is a square plate with the length, width and height of 8 × 1 cm.
The cell membrane sheet transfer device is characterized in that the height of the groove body is 0.2cm, the diameter of the groove body is 3.6cm, and 200ul of gel can be contained in the groove body.
The cell membrane sheet transfer device, wherein the handle has a height of 5 cm; the diameter of the circular truncated cone is 3.5 cm; the height of the circular truncated cone is 0.5 cm.
The cell membrane sheet transfer device is characterized in that the gel is in a gel shape at the temperature of less than or equal to 4 ℃;
the gel is in a liquid state at a temperature of more than or equal to 37 ℃.
The cell membrane sheet transfer device as described above, wherein the cover is circular and has an inner diameter of 4 cm.
Borrow by above-mentioned technical scheme, the utility model discloses a cell membrane piece transfer device has following advantage at least:
this cell membrane piece transfer device simple structure, convenient operation transports the cell with the form of diaphragm, has avoided traditional cell digestion to use the damage that the protease brought to cell and intercellular connection, has kept the extracellular matrix of cell autocrine and relevant protein and factor among the culture process, collects the cell with the complete membranous structure of one deck, has improved the utilization ratio of cell, provides suitable microenvironment for the growth of internal cell.
The basal layer of the cell patch is provided with the adhesion factors, so that the cell patch can be rapidly adhered to the surface of a bracket or a tissue without any sewing or connection during cell patch transplantation, which is very important for the construction of the cell patch in tissue engineering organs and fills the blank of the products in the market.
Meanwhile, the device is made of silicone materials, is light and small, and the cylindrical handle and the round table in threaded connection with the handle are connected in a detachable mode, so that the pollution to cells in the operation process is effectively avoided, and the safety of cell products is improved.
Drawings
Fig. 1 is a schematic structural view of a cell membrane sheet transfer device according to the present invention;
fig. 2 is another schematic structural diagram of a cell membrane sheet transport device according to the present invention;
description of reference numerals:
1. bottom plate 2, transfer device 3, lid
4. Trough body 5, gel 6 and handle
7. Round platform 8, anti-skid particles 9 and screw thread
Detailed Description
To further illustrate the technical means and effects of the present invention for achieving the objects of the present invention, the following detailed description of the cell membrane sheet transfer device according to the present invention, its specific embodiments, structures, features and effects will be made with reference to the accompanying drawings and preferred embodiments. In the following description, different "one embodiment" or "an embodiment" refers to not necessarily the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
As shown in fig. 1 and fig. 2, the cell membrane transferring device of the present invention comprises a bottom plate 1, a detachable transferring device 2, and a cover 3 of the transferring device 2; a circular groove body 4 is formed in the bottom plate 1, and gel 5 is filled in the groove body 4; the gel 5 is 7.5 wt% of pigskin gel 5; the transporter 2 comprises a cylindrical handle 6 and a circular truncated cone 7 connected with the handle 6 through threads 9; the cover body 3 can cover the circular truncated cone 7 and cover the circular truncated cone 7 inside.
The utility model discloses a cell membrane piece transfer device, it is at the in-process that uses:
culturing the cells on a temperature-sensitive culture dish when the culture dish is full of cells; putting 7.5 wt% of pig skin gel 5 into the tank 4, placing the round platform 7 of the transfer device and the handle 6 into the tank 4, keeping the tank at 4 ℃ for 30min, so that the gel 5 can be attached to the round platform 7 of the transfer device, placing the round platform 7 of the transfer device adhered with the gel on a temperature-sensitive culture dish, and placing the culture dish in an aseptic operation table at the temperature of (20-25 ℃) for 5min, so that cells can be detached from the culture dish in the form of a membrane and adsorbed on the gel 5 of the transfer device 2; then the transporter 2 and the cell membrane are placed in a new culture dish or a culture dish containing cells to be attached, the handle 6 is removed, the cover 3 is covered, the culture is cultured in an incubator at 37 ℃ for 30min, then the round table 7 is taken out, the culture solution is dripped, 7.5 wt% of the pig skin gel 5 is in a liquid state at the temperature of more than or equal to 37 ℃, the pig skin gel is melted and separated from the transporter together with the cell membrane, the cells are transported in the form of the membrane and are adhered to the new culture dish, the damage to the cells is avoided by adopting a physical method, and the utilization rate of the cells is improved.
7.5 wt% of the pigskin gel 5 is liquid at normal temperature, is jelly-shaped at 4 ℃, is matched with a temperature sensitive culture dish for use when the cell membrane sheet transfer device is used, cells are cultured in the temperature sensitive culture dish, the device is placed in the temperature sensitive culture dish when the membrane sheet needs to be prepared, is kept still for 5 minutes at room temperature (20-25 ℃), the cell membrane sheet is separated from the temperature sensitive culture dish and is adhered to the device, an operator places the device in a new culture dish to be cultured in a cell culture incubator for 30 minutes, the jelly-shaped gel 5 is dissolved in a culture solution and is separated from the device, and the cell membrane sheet can be adhered to the new culture dish or a tissue engineering bracket material.
The utility model discloses a cell membrane piece transfer device has guaranteed that low temperature transports, has reduced because the adverse effect that the temperature fluctuation caused the cell.
The utility model discloses a cell membrane piece transfer device, transfer device can transport together with cell matrix, and the cell of collecting has kept the extracellular matrix of external culture in-process secretion, the cell-matrix of establishing is connected and intercellular connection isotructure, has avoided protease digestion to collect the change of cell to the phenotype and the biochemical composition of cell, has also avoided destroying extracellular matrix and intercellular connexin simultaneously, has improved the utilization ratio of cell.
As shown in fig. 1 and fig. 2, in the cell membrane sheet transfer device of the present invention, the transfer device 2, the bottom plate 1 and the cover body 3 are all made of silicone material; the bottom plate 1 is a square plate with the length, width and height of 8 × 1 cm.
The utility model discloses a cell membrane piece transfer device, this transfer device adopt the preparation of silicone material, and is light small and exquisite, cylindric handle 6 and the round platform 7 of being connected with 6 screw threads of handle 9 adopt the detachable mode to be connected, and the effectual pollution to the cell in the operation process of having stopped has improved the security of cell class product.
As shown in fig. 1 and 2, the cell membrane sheet transfer device of the present invention has a height of the tank 4
0.2cm, 3.6cm in diameter, and 200ul of gel can be accommodated therein.
As shown in fig. 1 and 2, in the cell membrane sheet transfer device of the present invention, the height of the handle 6 is 5 cm; the diameter of the circular truncated cone 7 is 3.5 cm; the height of the circular truncated cone 7 is 0.5 cm.
As shown in fig. 1 and 2, in the cell membrane sheet transfer device of the present invention, the gel 5 is in the shape of gel 5 at a temperature of not more than 4 ℃; the gel 5 is in a liquid state at the temperature of more than or equal to 37 ℃.
7.5 wt% of the pigskin gel 5 is liquid at normal temperature, namely the temperature is more than or equal to 37 ℃, and is jelly-shaped at the temperature of 4 ℃;
the gel 5 used in the utility model is the gel 5 with the product number of 48722-100G-F produced by Sigma company, and the gel 5 circulates on the market and can be directly purchased and used by the technicians in the field.
As shown in fig. 1 and 2, the cover 3 of the cell membrane sheet transport device of the present invention is circular and has an inner diameter of 4 cm.
As shown in fig. 1 and 2, the cell membrane sheet transferring device of the present invention, a holding portion is disposed on the sidewall of the handle 6, and anti-slip particles 8 are disposed thereon, so that the user can hold the cell membrane sheet transferring device for operation.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and any simple modification, equivalent change and modification made by the technical spirit of the present invention to the above embodiments are all within the scope of the technical solution of the present invention.
Claims (6)
1. A cell membrane sheet transfer device, comprising:
comprises a bottom plate, a detachable transporter and a cover body of the transporter;
the bottom plate is provided with a circular groove body, and gel is filled in the groove body; the gel is 7.5 wt% of pigskin gel;
the transfer device comprises a cylindrical handle and a circular truncated cone in threaded connection with the handle;
the cover body can cover the round platform and cover the round platform inside the cover body.
2. The cell membrane sheet transport device according to claim 1, wherein:
the transporter, the bottom plate and the cover body are all made of silicone materials;
the bottom plate is a square plate with the length, width and height of 8 × 1 cm.
3. The cell membrane sheet transport device according to claim 2, wherein:
the height of the groove body is 0.2cm, the diameter is 3.6cm, and 200ul of gel can be accommodated in the groove body.
4. The cell membrane sheet transport device according to claim 3, wherein:
the height of the handle is 5 cm; the diameter of the circular truncated cone is 3.5 cm; the height of the circular truncated cone is 0.5 cm.
5. The cell membrane sheet transport device according to claim 4, wherein:
the gel is in a gel state at the temperature of less than or equal to 4 ℃;
the gel is in a liquid state at a temperature of more than or equal to 37 ℃.
6. The cell membrane sheet transport device according to claim 5, wherein:
the lid is circular, and its internal diameter is 3.8 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920694130.8U CN210560363U (en) | 2019-05-16 | 2019-05-16 | Cell membrane piece transfer device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920694130.8U CN210560363U (en) | 2019-05-16 | 2019-05-16 | Cell membrane piece transfer device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210560363U true CN210560363U (en) | 2020-05-19 |
Family
ID=70625652
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920694130.8U Expired - Fee Related CN210560363U (en) | 2019-05-16 | 2019-05-16 | Cell membrane piece transfer device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210560363U (en) |
-
2019
- 2019-05-16 CN CN201920694130.8U patent/CN210560363U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200519 Termination date: 20210516 |
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| CF01 | Termination of patent right due to non-payment of annual fee |